In Central Amazonia, large mammals create water-filled puddles when consuming soil. These mineral licks are visited by pregnant and lactating frugivorous bats; possibly for two reasons. Frugivorous bats could supplement their mineral-depleted fruit diet by drinking salty water, or they could buffer dietary plant secondary compounds by consuming soil. We analysed bat fruits from Ecuador and showed that they are depleted in elemental concentrations (Na, K, P) compared with similar fruits collected from Costa Rica, where no mineral licks occur (n = 32). Analyses of water from Ecuador revealed that water samples from six mineral licks contained more physiologically relevant elements (Na, K, Mg, Ca) than four samples from river and stream water control sites (Mann–Whitney U-test). In support of the nutrient supplement hypothesis, we observed bats drinking mineral-enriched water at these licks (video observation). Furthermore, blood collected from 68 bats differed in composition with respect to physiologically relevant minerals (Na, K, Mg, Fe) from that of frugivorous bats captured at control sites. To test whether frugivorous bats also consumed clay for detoxification, we checked for soil tracer elements in 31 faecal samples. Soil tracers are insoluble in water and, thus, are not included in a strict fruit diet. Bats from mineral licks showed higher aluminium soil tracer concentrations in their faeces than bat species that never visit licks, suggesting that frugivorous bats take up clay material at mineral licks. Our results provide evidence that frugivorous bats ingest soluble mineral nutrients and insoluble soil by consuming soil-enriched water at mineral licks, thus supporting the hypothesis that frugivorous bats of western Amazonia may derive a dual benefit from drinking water from mineral licks.

Vertical stratification of vascular epiphytes is frequently attributed to niche partitioning along microclimatic gradients but experimental confirmations of this notion are rare. This study investigates the role of the regeneration phase for the stratification of five bromeliad (Catopsis sessiliflora, Guzmania subcorymbosa, Tillandsia anceps, T. bulbosa and Werauhia gladioliflora) and seven aroid species (Anthurium acutangulum, A. brownii, A. clavigerum, A. durandii, A. friedrichsthalii, A. hacumense and A. scandens) in a Panamanian rain forest. We documented gradients of temperature, vapour pressure deficit and light (n = 10 d) as well as species height distributions (n = 11–120). Microclimatic gradients were substantial (maximal T and RH differences between strata: 5 °C and 18%, respectively) and mean attachment heights of the study species (range = 4–21 m) differed significantly. We tested sensitivity to recurrent drought (four treatments) during germination (all species, cumulative germination of 20 seeds, n = 3) and seedling growth (four aroid species, n = 25). Seedling survival of six aroid species transplanted to three heights (n = 27) was monitored in situ. Some species did not germinate under severe recurrent drought while others germinated at the same rate in all treatments. Seedlings of the most exposed species grew fastest under intermediate recurrent drought while those of the other three species grew fastest when kept constantly wet. Survival of transplanted seedlings did not depend on species attachment height, but this may be attributable to insufficient statistical power. Taken together, the results suggest that the stratification can be explained to a large degree by differential sensitivity to the vertical moisture gradient during the regeneration phase.

We documented one of the most species-rich assemblages of tropical rain-forest Auchenorrhyncha, comprising 402 phloem- and xylem-feeding species, by sampling adults from forest vegetation. Further, we reared 106 species from larvae sampled on 14 plant species. Both xylem- and phloem-feeding guilds exhibited wide host-plant ranges, as 74% of species fed on more than one plant family. In comparison, using data extracted from the temperate-zone literature, phloem-feeders exhibited lower host specificity in Papua New Guinea than in Germany, because in Papua New Guinea they were dominated by generalist Fulgoroidea while in Germany by specialist Membracoidea. The similarity of Auchenorrhyncha assemblages from different plant species was unrelated to the phylogenetic distance between their hosts. Host specificity, abundance and species composition of Auchenorrhyncha assemblages were unrelated to the optimum of their host plant species on succession gradient from secondary to primary forest. Higher host specificity did not lead to greater species richness in Auchenorrhyncha assemblages feeding on different plant species, but the number of species feeding on a particular plant species was a strong predictor of the Auchenorrhyncha abundance on that plant. These patterns suggest that Auchenorrhyncha assemblages on these plant species are not saturated with species and determined by division of limited resources among competitors, but instead are dependent on the number of colonizers from the regional species pool.

Of all feeding guilds, understorey insectivores are thought to be most sensitive to disturbance and forest conversion. We compared the composition of bird feeding guilds in tropical forest fragments with adjacent agro-ecosystems in a montane region of south-west Ethiopia. We used a series of point counts to survey birds in 19 agriculture and 19 forest sites and recorded tree species within each farm across an area of 40 × 35 km. Insectivores (~17 spp. per plot), frugivores (~3 spp. per plot) and omnivores (~5 spp. per plot) maintained species density across habitats, while granivores and nectarivores increased in the agricultural sites by factors of 7 and 3 respectively. Species accumulation curves of each guild were equal or steeper in agriculture, suggesting that agricultural and forest landscapes were equally heterogeneous for all bird guilds. Counter to most published studies, we found no decline in insectivore species richness with forest conversion. However, species composition differed between the two habitats, with certain forest specialists replaced by other species within each feeding guild. We suggest that the lack of difference in insectivorous species numbers between forest and agriculture in this region is due to the benign nature of the agricultural habitat, but also due to a regional species pool which contains many bird species which are adapted to open habitats.

Survival of Afrotropical primary forests depends not only on habitat protection but also on the protection of animal species such as frugivorous primates, recognized as the most important seed dispersers for many plants. Here we investigate seed-dispersal services by the bonobo (Pan paniscus) in an evergreen lowland tropical rain forest of the Congo Basin. In the long-term research site of LuiKotale, we investigated food habits and seed processing based on 22 mo of behavioural observation, seed trial experiment and long-term daily GPS tracking of a habituated ape community. Bonobos were mainly frugivores (66% of all feeding sessions), spending about 3.5 h d−1 swallowing seeds that were transported for an average of 24 h in the gut. On average, an individual bonobo dispersed 172 kg y−1 of seeds (or 220000 seeds y−1) of more than 91 plant species by endozoochory over an average distance of 1.2 km from the parent tree. Passed seeds germinated more rapidly, more successfully and had greater post-dispersal survival than unpassed seeds. Bonobo-dispersed plants accounted for 40% of tree species and 65% of individual trees in the study site (12 1-ha plots census). Since bonobos show little functional overlap with other frugivores, the loss of their seed-dispersal services is likely to affect forest structure and dynamics. Our results justify description of the threatened bonobo as a gardener of the Congo forest.

Convergence in the use of resources may occur between distantly related organisms. A major ecological resource in which members of various taxa may be interested is a cavity for nesting. A variety of social hymenopterans and vertebrates may nest within tree cavities in tropical ecosystems. We used 241 nest-boxes placed in seven Kenyan localities to investigate the use of nesting cavities by members of distant taxa and discuss whether interaction between them is a potential factor shaping cavity-nester communities in tropical regions. The nest-boxes were occupied by social insects (ants, bees and wasps) (30.1% of nest-boxes in April–May and 33.1% in September–October) and vertebrates (birds and mammals) (20% and 7.7%, respectively). Hymenopterans were more abundant in forest boxes (36.2% of nest-boxes occupied in April–May and 37% in September–October), whereas savannas had lower figures (21.7% and 31.3%, respectively). Among vertebrates, most occupants of nest-boxes in savanna were birds (17.8% of nest-boxes occupied vs. 8% in mammals), while mammals predominated in forests (4.9% of the nest-boxes occupied vs. 0.3% in birds). Spatial and temporal patterns of occupation highlight the potential that interaction between distant taxa may have on the access to nesting cavities. More nest-boxes remained unoccupied in forested areas than in savanna areas suggesting that a shorter supply of nesting sites in the savanna may be a source of competition. The simultaneous occupation of a nest-box by two different taxa was exceptional, also supporting the hypothesized inter-taxon competition.

Major tropical rivers have been suggested to be important dispersal barriers that increase the beta diversity of animal communities in lowland rain forests. We tested this hypothesis using assemblages of frogs in the floodplains of the Sepik River, a major river system in Papua New Guinea. We surveyed frogs at five sites within a continuous 150 × 500-km area of lowland rain forest bisected by the Sepik, using standardized visual and auditory survey techniques. We documented 769 frogs from 44 species. The similarity in species composition decreased with logarithm of geographical distance between the sites, which ranged from 82 to 465 km. The similarity decay did not depend on whether or not the compared sites were separated by the Sepik River or whether the species were aquatic or terrestrial breeders. Likewise, a DCA ordination of frog assemblages did not show separation of sites by the river as a significant factor explaining their composition. Our results suggest that even major rivers, such as the Sepik, may not act as dispersal barriers. Rivers may not limit the distribution of frogs and therefore have a limited effect on determining frog species abundance and assemblage structure in rain forests.

Neotropical streams are losing dominant consumer groups as a result of disease-driven amphibian declines. The herbivorous tadpoles of Lithobates warszewitschii were once abundant in the Rio Maria in the Eastern Cordillera Central of Panama, where they consumed algae and organic matter. The decline of this once abundant grazer has the potential to affect the resources consumed by insect grazers in this system. Stable isotopes were used to characterize changes in the resource use before and after amphibian declines of four abundant insect grazer taxa: Stenonema spp., Thraulodes spp., Psephenus spp. and Petrophila spp. We collected 11 isotope samples of L. warszewitschii and 27 isotope samples of these insect taxa in 2006, and then 24 more isotope samples of the same insect taxa in 2008, 20 mo. after a disease-driven amphibian extirpation. We also tested for potential functional redundancy of insects with tadpoles by comparing the post-decline isotopic niche of each insect taxon to the isotopic niche of L. warszewitschii. The isotopic niche of Psephenus spp., Petrophila spp. and Stenonema spp. shifted from 2006 to 2008, but none of the insect taxa in 2008 occupied the same isotopic niche as tadpoles. Our study builds on previous evidence that the ecological roles of tadpoles were not replaced through functional redundancy after amphibian declines.

Species of Miconia are considered keystone plant resources for frugivorous birds in Neotropical forests, but for other ecosystems little of their ecological role is known. The fruiting phenology and the composition of frugivores of four Miconia species in savanna and palm swamp from the Brazilian Neotropical savanna were studied in three sites from November 2005 to May 2011. The hypothesis tested was that plants from different habitats share their frugivores and consequently promote links between habitats. Through focal plant observations (30–50 h per species in each site), 668 visits by 47 species of birds were recorded and plants from different habitats shared most of the frugivores (49–97%). The fruiting of Miconia chamissois in the palm swamp during the period of fruit scarcity (dry season) was accompanied by an enhancement in the frugivore bird richness and abundance in this habitat, providing indirect evidence of resource tracking. Bird species which primarily dwell in savanna recorded consuming fruits in palm swamps during the resource-scarce season is taken as evidence of landscape supplementation. Miconia assemblage studied here seems to promote a link between two adjacent habitats in the Neotropical savanna from Central Brazil, a link which is likely to be common in this naturally patchy ecosystem.

Invasive weeds like Lantana camara have a range of effects on animals such as elephant. These plants are not edible by the Asian elephant (Elephas maximus). They also compete for space with elephant food plants and take over large areas of elephant habitat. We tested whether the addition of L. camara to a model consisting of measured environmental variables improved predictions of habitat use by elephant in Mudumalai Tiger Reserve, India. Elephant dung density was used to assess elephant habitat use from 62 line transects 1-km in length. Results indicated that habitat and impact of human settlements significantly influenced elephant habitat use at a landscape scale. However, we found no evidence for the hypothesis that the addition of L. camara significantly predicted elephant habitat use at the landscape level. We then tested the association of L. camara on elephant habitat use in the dry deciduous forest (DDF) where there was a significant interaction between DDF and L. camara. In the DDF, L. camara significantly predicted elephant habitat use. We conclude that while no significant effects of L. camara were seen at the level of an entire reserve, at a finer scale and in specific habitats negative effects of this invasive plant on elephant habitat use were observed.

The movement patterns of males, females and juveniles of lekking species often differ due to differences in the commitment to lek activities, which may lead to differences in the spatial distribution and dispersal distances of seeds they eat. By sampling seeds in three lek and non-lek areas of the white-bearded manakin (Manacus manacus), we tested whether this lekking species increased the abundance and species richness of seeds in lek areas and, at a finer scale, in 21 displaying courts within lek areas. Combining data on seed defecation or regurgitation rates by free-ranging individuals, the number of seeds in droppings or regurgitations of mist-netted birds, and the distances travelled by birds equipped with radio-transmitters, we estimated the potential spatial distribution of seeds generated by six resident males and six females or juveniles during the morning peak of lek activity and when lek activity decreased in the afternoon. There was no difference in the species richness (46 and 44 morphospecies, respectively) and abundance of seeds (15.4 ± 7.3 seeds and 14.0 ± 1.1 seeds, respectively) between lek and non-lek areas. Within leks both parameters increased in courts (45 spp., 17.6 ± 14 seeds) compared with non-court sites (22 spp., 1.9 ± 1.8 seeds), likely as a consequence of the longer time spent by resident males in perches in or near display courts. Distances moved by juveniles and females per 60-min period (183 ± 272 m) were greater than resident males (42.6 ± 22.0 m) in the mornings, while the opposite happened in the afternoons (55.2 ± 40.7 m and 157 ± 105 m, respectively). We conclude that the spatial aggregation of seeds in lek areas of M. manacus occurs at the court level, and the spatial distribution of deposited seeds varies with manakin lekking status and the daily period of foraging.

Termites are renowned ecosystem engineers. Their mounds have been described as an important element of savanna vegetation dynamics, but little is known about their large-scale impact on vegetation composition. To investigate the influence of termite-induced heterogeneity in savannas along a climatic gradient in West Africa termite mound vegetation was compared with adjacent savanna vegetation using 256 paired plots (size of the termite mound and a corresponding savanna area) in five protected areas from northern Burkina Faso to northern Benin. On each plot vegetation and soil sampling was performed. Additionally bioclimatic variables from the WORLDCLIM database were used. The vegetation on the mounds and the surrounding savanna differed within all study sites (DCA length of gradient 3.85 SD) and showed complete turnover along the climatic gradient (DCA length of gradient 5.99 SD). Differences between mounds and savanna were significantly related to termite-induced changes in soil parameters, specifically clay enrichment and increased cation concentrations (base saturation). On a local scale, termite-induced differences in soil conditions were found to be the most important factor affecting mound vegetation, while on a regional scale, annual precipitation showed the strongest significant correlations. However, with increasing precipitation, differences between mounds and the surrounding matrix became more pronounced, and the contribution of mounds to local phytodiversity increased. Eleven plant species were identified as characteristic termite mound species. In the more humid parts of the gradient, more characteristic plant species were found that may benefit from favourable soil conditions, good water availability, and a low fire impact in the mound microhabitat.

Plant survival and growth in tropical rain forest are affected by different biotic and abiotic forces. As time elapses and plants grow the relative importance of such forces as regeneration inhibitors and/or facilitators may change according to habitat and species. To detect within- and among-species divergences in performance over time in different habitats we followed, for nearly a decade, the survival, growth and herbivory of seedlings of the native tree species: Chrysophyllum pomiferum, Micropholis venulosa and Pouteria caimito. In Central Amazonia, young seedlings were planted into old-growth and secondary forests dominated by Vismia spp. One year after planting, C. pomiferum ranked first (i.e. fast growth, fewer dead and less herbivory) for both habitats, followed by M. venulosa and P. caimito. Initial trends changed over time. In the long term, M. venulosa ranked first for both habitats, followed by C. pomiferum and P. caimito ranked consistently lowest. Within-species divergences in growth and herbivory were greater in secondary forest. Initial seedling responses cannot always be used to predict species persistence in the long term. Contrary to previous estimations, old-growth-forest species can persist under Vismia spp. stands, at least when planted.

Forest fragmentation can alter herbivory on tree recruits with possible consequences for regeneration. We assessed effects of forest-fragment quality (tree diversity, vegetation complexity, relative abundance of pioneer trees) and matrix habitat on arthropods and herbivory in KwaZulu-Natal, South Africa. We compared arthropod abundances and herbivory on woody seedlings and saplings among four forest-fragment types differing in size and matrix (large fragments and small fragments surrounded by natural grassland, eucalypt and sugarcane plantations; nplots = 24) using analyses of covariance. We recorded 3385 arthropods and inspected 897 seedlings (71 species) and 876 saplings (91 species). Relative abundance of predators increased with fragment quality; that of herbivores decreased. Herbivory responses to fragment quality varied: seedling herbivory decreased with relative abundance of pioneers and sapling herbivory increased with vegetation complexity. Matrix effects were low with little variation in relative abundance of predators (0.39–0.53) and herbivores (0.22–0.32), proportion of seedling (8.3–11.0%) and sapling herbivory (12.4–14.3%) among the forest-fragment types. These findings indicate that herbivory on tree recruits is mediated by forest-fragment quality rather than matrix habitat. Future studies should evaluate whether contrasting effects of fragment quality on arthropods and herbivory are caused by weak trophic interactions and variable herbivore compositions.

Isolated pioneer trees have been shown to increase the deposition of animal-dispersed seeds. The effect of gender on seed rain has not yet been investigated, and this study aimed to evaluate whether female and male plants of a pioneer dioecious zoochoric tree differ with respect to the seed rain under their canopies. Seed rain was evaluated for 13 mo, from October 2009 to October 2010, in secondary vegetation of the Atlantic forest in southern Brazil. We used 60 seed traps (0.5 m2): 40 traps under the crowns of 40 Myrsine coriacea (Primulaceae) trees (20 male and 20 female individuals) and 20 at sites without trees. We found 365071 diaspores belonging to 115 morphospecies from 37 families, and 38.3% of the morphospecies were trees, most of them zoochoric. The female trees accumulated a greater number of diaspores and species richness than male trees. The male trees accumulated a higher number of seeds and species than areas without trees. This study shows that sites containing female and male trees of M. coriacea and sites without trees differed significantly in terms of seed rain, and there is a gender effect in this dioecious, pioneer tree species because female plants promote an increase in the richness and abundance of diaspores.

In New Caledonia, rain forests with an upper canopy dominated by single species of Nothofagus occur next to mixed-canopy forests, without discernible environmental cause. A potential explanation is that they are different successional stages. To test this hypothesis and predict long-term change in canopy dominance, population size structures of 61 canopy species were analysed in six Nothofagus-dominated forests and three adjacent mixed rain forests. Weibull analysis suggests that these Nothofagus forests are secondary forests, with recruitment insufficient to maintain monodominance, except at a high-altitude site. At low- to mid-altitudes the Nothofagus canopy is predicted to develop into a mixed canopy, unless moderate to severe disturbance occurs within its reproductive lifespan. However, adjacent mixed rain forests are also secondary, with 85% of analysed species showing no evidence of continuous regeneration. Fifteen species from both forest types showed reverse-J curves suggesting continuous regeneration, but only Calophyllum caledonicum did so consistently. Since few canopy species showed evidence of high shade tolerance and persistence, a small number of shade-tolerant species is predicted to dominate both forests in the long term, in the hypothetical absence of disturbance. Hence, temporal factors associated with disturbances play a key role in determining dominance in these forests.

Hurricanes are major disturbance events in Neotropical mangrove communities, potentially affecting the reproductive success of mangrove species. This was the first investigation of changes in mangrove pollinator assemblages following hurricanes, and the effect of these changes on the mating system of Laguncularia racemosa. Insect pollinator assemblages were investigated in three Florida mangrove communities in 2001–2003, 2005 and 2009; two hurricanes affected the area in 2004. Visitation rates were estimated from 1445 insects observed during 272 10-min intervals; the number of flowers visited by each insect was also recorded. Pollinator diversity was estimated with the Shannon Index. Following the hurricanes, species richness was reduced by 43–65% and diversity declined by 36–70%. Significant declines in insect visitation to L. racemosa resulted in reduced outcrossing frequencies in 2005. Laguncularia racemosa flowers autogamously self-pollinate without insect visitors, so fruit set still occurred. Visitation rates returned to pre-hurricane levels by 2009, but foraging behaviours differed from pre-hurricane patterns; outcrossing was further favoured by reduced frequencies of long foraging bouts and increased frequencies of short foraging bouts. The mixed mating system of L. racemosa provides reproductive assurance following hurricane disturbances, when pollinator abundance is low.

Savannas intermingled with gallery forests are dynamic habitats typical in Africa. This study aims to determine if differences in species traits lead to non-overlapping distribution of gallery-forest and savanna species and abrupt transition between gallery forest and savanna. Tree species densities were measured in 375 plots of 1500 m2 covering a total sample area of 56.25 ha along forty 3-km transects located at right angles to a riverbed with gallery forest into surrounding savanna. Location, vegetation type, soil physical properties, erosion and fire occurrence were recorded as site factors. Data analysis included the quantification of co-occurrence patterns, threshold indicator taxa analysis and fuzzy set ordination. The gallery forest–savanna gradient predicted floristic composition of plots with a correlation of 0.595 but its accuracy was locally modified by the occurrence of fire and the physical properties of soil that covered more than 30% of the range of residuals. The distribution of gallery-forest and savanna tree species did not overlap. Along the gallery forest–savanna gradient, savanna species gradually increased in density while gallery-forest species showed a community threshold at 120 m from the river beyond the width of gallery forest. The forest species driving this trend should play an important role in the dynamics of gallery forest–savanna boundaries.

To investigate the influence of forests and agroforestry systems on fruit consumption by birds, we studied two landscapes, one covered predominantly with forests and the other dominated by traditional shade cocoa plantations. In each landscape, we sampled three forest fragments and three shade cocoa plantations. We placed 15 artificial fruits in 25, 1–2-m-tall shrubs spaced every 50 m and evaluated the detection and consumption of fruits after 72 h. We used hemispherical photographs positioned above each fruit station to evaluate canopy openness. We found a statistically significant difference in fruit consumption between landscapes, which means that more fruits were detected and consumed in the forest-dominated landscape. However, forests and shade cocoa plantations within each landscape exhibited similar fruit consumption. Canopy openness was similar between the landscapes, however, the cocoa plantations exhibited greater canopy openness than forests. The results of this study reinforce the importance of the presence of forests in the agricultural landscape. Thus, to evaluate the capacity of agroforest to protect species and maintain ecological interactions it is also necessary to consider the landscape context.

Fig trees (Ficus spp) produce fruit year-round and figs are consumed by a large proportion of frugivores throughout the tropics. Figs are potential keystone resources that sustain frugivore communities during periods of scarcity, but studies have produced contradictory results. Over 1 y we monitored the phenology of 206 trees of five Ficus species in a Colombian cloud forest, to test whether figs produced fruit during periods of low overall fruit availability. We also measured fig tree densities in 18 0.5-ha plots and made 190 h of observations at 24 trees of three species to determine whether figs were abundant and consumed by a large proportion of the local frugivores. The five species produced fruit year-round but fig availability varied monthly by orders of magnitude. Fig trees reached comparatively high densities of 1–5 trees ha−1 and were consumed by 36 bird species (60% of the local frugivore assemblage) and three mammal species. However, there was no season of fruit scarcity and figs represented on average 1.5% of the monthly fruit biomass. Figs in this Andean forest are part of a broad array of fruiting species and at least during our study did not seem to constitute a keystone resource.