The objective of the present study was to test the reliability of the analysis method of the reflectance spectrum of fat in the zone of light absorption by carotenoid pigments developed in lambs to trace grass feeding in calves. Six feeding treatments were compared in suckling calves: ad libitum cocksfoot hay plus ad libitum concentrate or half level of ad libitum concentrate (HH and HL), ad libitum cut grass plus ad libitum concentrate or half level of ad libitum concentrate (GH and GL), ad libitum highland grazed grass plus ad libitum concentrate (PH); ad libitum medium quality cocksfoot hay plus dam's milk (C0). Groups HH, HL, GH, GL and PH were slaughtered at approximately 10 months of age, after 150 days of experimental feeding diet. C0 calves were slaughtered at the beginning of the experiment at approximately 6 months of age. The concentration of carotenoids was analysed by HPLC on diets and calves' plasma at slaughter. The reflectance spectrum of perirenal and subcutaneous fat were measured at slaughter and expressed as AVI units. Plasma carotenoid content was higher (P<0·05) in PH and GL calves than in HH, HL and GH calves. There was an effect (P<0·05) of feeding treatment and measurement site on AVI. Values obtained for perirenal fat were higher (P<0·05) than those obtained for subcutaneous fat. For plasma carotenoid content and for AVI values in both fat tissues there was no overlapping between hay-fed calves on the one hand and GL or grazing calves on the other hand. However, there was some overlapping between hay-fed calves and GH calves. For plasma carotenoid content, values for C0 calves overlapped with values of GH and GL calves. For both fat tissues AVI values of C0 calves overlapped with values of HH, HL, GH and GL calves. AVI of cut grass fed calves (GH and GL groups) increased linearly (P<0·05) with average daily grass intake for both tissues considered. AVI in subcutaneous fat tissue decreased linearly (P<0·05) with average daily concentrate intake but this relation was not significant for AVI in perirenal fat (P>0·05). Plasma carotenoid concentration and the reflectance spectrum of the fat in the zone of light absorption by carotenoid pigments could be used to trace grass feeding in calves. Results obtained in this study suggest that different fatty tissues could differ in their reliability and interest for traceability of grass-feeding, depending on the animal age and production system.

Prenatal development is known to be extremely sensitive to maternal and environmental challenges. In this study, we hypothesize that body growth and lactation during gestation in cattle reduce nutrient availability for the pregnant uterus, with consequences for placental development. Fetal membranes of 16 growing heifers and 27 fully grown cows of the Belgian Blue (BB) breed were compared to determine the effect of body growth on placental development. Furthermore, the fetal membranes of 49 lactating Holstein Friesian (HF) cows and 27 HF heifers were compared to study the impact of dam lactation compared to dam body growth. After parturition, calf birth weight and body measurements of dam and calf were recorded, as well as weight of total fetal membranes, cotyledons and intercotyledonary membranes. All cotyledons were individually measured to calculate both the surface of each individual cotyledon and the total cotyledonary surface per placenta. Total cotyledonary surface was unaffected by breed or the breed×parity interaction. Besides a 0.3 kg lower cotyledonary weight (P=0.007), heifer placentas had a smaller total cotyledonary surface compared with placentas of cows (0.48±0.017 v. 0.54±0.014 m2, respectively, P<0.001). Within the BB breed, fetal membranes of heifers had a 1.5 kg lower total weight and 1.0 kg lower intercotyledonary membrane weight (P<0.005) compared with cows. A cotyledon number of only 91±5.4 was found in multiparous BB dams, while growing BB heifers had a higher cotyledon number (126±6.7, P<0.001), but a greater proportion of smaller cotyledons (<40 cm2). Within the HF breed, no parity effect on intercotyledonary membrane weight, cotyledon number and individual cotyledonary surface was found. Placental efficiency (calf weight/total cotyledonary surface) was similar in HF and BB heifers but significantly higher in multiparous BB compared with multiparous HF dams (106.0±20.45 v. 74.3±12.27 kg/m2, respectively, P<0.001). Furthermore, a seasonal effect on placental development was found, with winter and spring placentas having smaller cotyledons than summer and fall placentas (P<0.001). Main findings of the present study are that lactation and maternal growth during gestation entail a comparable nutrient diverting constraint, which might alter placental development. However, results suggest that the placenta is able to manage this situation through two potential compensation mechanisms. In early pregnancy the placenta might cope by establishing a higher number of cotyledons, while in late gestation a compensatory expansion of the cotyledonary surface is suggested to meet the nutrient demand of the fetus.

Milk fatty acid (FA) profile has been previously used as a predictor of enteric CH4 output in dairy cows fed diets supplemented with plant oils, which can potentially impact ruminal fermentation. The objective of this study was to investigate the relationships between milk FA and enteric CH4 emissions in lactating dairy cows fed different types of forages in the context of commonly fed diets. A total of 81 observations from three separate 3×3 Latin square design (32-day periods) experiments including a total of 27 lactating cows (96±27 days in milk; mean±SD) were used. Dietary forages were included at 60% of ration dry matter and were as follows: (1) 100% corn silage, (2) 100% alfalfa silage, (3) 100% barley silage, (4) 100% timothy silage, (5) 50 : 50 mix of corn and alfalfa silages, (6) 50 : 50 mix of barley and corn silages and (7) 50 : 50 mix of timothy and alfalfa silages. Enteric CH4 output was measured using respiration chambers during 3 consecutive days. Milk was sampled during the last 7 days of each period and analyzed for components and FA profile. Test variables included dry matter intake (DMI; kg/day), NDF (%), ether extract (%), milk yield (kg/day), milk components (%) and individual milk FA (% of total FA). Candidate multivariate models were obtained using the Least Absolute Shrinkage and Selection Operator and Least-Angle Regression methods based on the Schwarz Bayesian Criterion. Data were then fitted into a random regression using the MIXED procedure including the random effects of cow, period and study. A positive correlation was observed between CH4 and DMI (r=0.59, P<0.001), whereas negative associations were observed between CH4 and cis9-17:1 (r=−0.58, P<0.001), and trans8, cis13-18:2 (r=−0.51, P<0.001). Three different candidate models were selected and the best fit candidate model predicted CH4 with a coefficient of determination of 0.84 after correction for cow, period and study effects and was: CH4 (g/day)=319.7−57.4×15:0−13.8×cis9-17:1−39.5×trans10-18:1−59.9×cis11-18:1−253.1×trans8, cis12-18:2−642.7×trans8, cis13-18:2−195.7×trans11, cis15-18:2+16.5×DMI. Overall and linear prediction biases of all models were not significant (P>0.19). Milk FA profile and DMI can be used to predict CH4 emissions in dairy cows across a wide range of dietary forage sources.

Animal temperament is a trait of economic relevance and its use as a selection criterion requires the identification of environmental factors that influence this trait, as well as the estimation of its genetic variability and interrelationship with other traits. The objectives of this study were to evaluate the effect of the covariates dam age at calving (ADC), long yearling age (YA) and long yearling weight (YW) on temperament score (T) and to estimate genetic parameters for T, scrotal circumference (SC) at long YA and age at first calving (AFC) in Nellore cattle participating in a selection program. The traits were analyzed by the restricted maximum likelihood method under a multiple-trait animal model. For all traits, contemporary group was included as a fixed effect and additive genetic and residual as random effects. In addition to these effects, YA, YW and ADC were considered for analyzing T. In the case of SC and AFC, the effect of long YW was included as a covariate. Genetic parameters were estimated for and between traits. The three covariates significantly influenced T. The heritability estimates for T, SC and AFC were 0.18 ± 0.02, 0.53 ± 0.04 and 0.23 ± 0.08, respectively. The genetic correlations between T and SC, and T and AFC were −0.07 ± 0.17 and −0.06 ± 0.19, respectively. The genetic correlation estimated between SC and AFC was −0.57 ± 0.16. In conclusion, a response to selection for T, SC and AFC is expected and selection for T does not imply correlated responses with the other traits.

Methane (CH4) production is a ubiquitous, apparently unavoidable side effect of fermentative fibre digestion by symbiotic microbiota in mammalian herbivores. Here, a data compilation is presented of in vivo CH4 measurements in individuals of 37 mammalian herbivore species fed forage-only diets, from the literature and from hitherto unpublished measurements. In contrast to previous claims, absolute CH4 emissions scaled linearly to DM intake, and CH4 yields (per DM or gross energy intake) did not vary significantly with body mass. CH4 physiology hence cannot be construed to represent an intrinsic ruminant or herbivore body size limitation. The dataset does not support traditional dichotomies of CH4 emission intensity between ruminants and nonruminants, or between foregut and hindgut fermenters. Several rodent hindgut fermenters and nonruminant foregut fermenters emit CH4 of a magnitude as high as ruminants of similar size, intake level, digesta retention or gut capacity. By contrast, equids, macropods (kangaroos) and rabbits produce few CH4 and have low CH4 : CO2 ratios for their size, intake level, digesta retention or gut capacity, ruling out these factors as explanation for interspecific variation. These findings lead to the conclusion that still unidentified host-specific factors other than digesta retention characteristics, or the presence of rumination or a foregut, influence CH4 production. Measurements of CH4 yield per digested fibre indicate that the amount of CH4 produced during fibre digestion varies not only across but also within species, possibly pointing towards variation in microbiota functionality. Recent findings on the genetic control of microbiome composition, including methanogens, raise the question about the benefits methanogens provide for many (but apparently not to the same extent for all) species, which possibly prevented the evolution of the hosting of low-methanogenic microbiota across mammals.

Estimates of co(variance) components were obtained for weights at birth, weaning and 6, 9 and 12 months of age in Muzaffarnagari sheep maintained at the Central Institute for Research on Goats, Makhdoom, Mathura, India, over a period of 28 years (1976 to 2003). Records of 5530 lambs out of 169 rams and 1631 ewes were used for the study. Analyses were carried out by restricted maximum likelihood (REML), fitting six animal models, including various combinations of maternal effects. Heritability estimates for weight at birth, weaning and 6, 9 and 12 months of age were 0·09, 0·21, 0·06, 0·10 and 0·14, respectively. Maternal heritability of body weight declined from 0·07 at birth to 0·02 at weaning. The maternal permanent environmental component contributed 10 to 11% to the total variance for all traits except weight at 12 months. A significant large negative genetic correlation was observed between direct and maternal genetic effects on weaning weight, suggesting the presence of antagonistic environmental, as well as perhaps genetic, effects in daughter and dam. Results suggest that maternal additive effects were only important in early stages of growth, whereas a permanent environmental maternal effect existed at all ages up to 9 months, probably as a carry-over effect of maternal influences present at weaning. Modest rates of genetic progress appear possible for all weights, but the presence of an antagonism between direct and maternal effects on weaning weight would complicate attempts to improve both by selection.

One hundred 1-day-old Ven Cobb chicks were used to study the effect of supplementation with 0-2 mg chromium (Cr) per kg diet from potassium chromate (T1), chromium chloride (T2) and chromium-yeast complex (T3). The control group (C) received no supplemental chromium. Each experimental group consisted of five replicates of five birds each and the supplementation was continued for 35 days. The weekly live-weight gain, food conversion ratio and the live-weight gain: food intake ratio were unaffected by the treatments. Metabolizability of the organic nutrients increased in the Cr supplemented groups compared with the C group and significantly higher values were observed in the T3 group indicating beneficial effects of Cr-yeast complex. Intake of the trace elements (copper, zinc, iron and manganese) fulfilled the requirements specified for broiler chickens. Retention of all these trace elements was higher (P < 0-001 for Cu, Zn and Fe, P <0-01 for Mn) in all the Cr supplemented groups compared with that in the C group. Furthermore, in the T3 group the retention of copper, zinc, iron and manganese was higher (P < 0001) than that in the T1 and T2 groups. Two birds were slaughtered from each replicate at the end of 21 and 35 days of feeding to observe Cr in the liver; this was higher (P < 0-01) after 35 days feeding only. Despite supplementation, liver Cr was lower (P < 0-01) in T1 T2 and T3 groups. Cr concentration in the plasma was higher (P < 0-05) in the T3 and T3 groups than that in the C group although its concentration did not change with the age of the birds. Higher (P < 0-01) plasma concentrations of copper were observed in the T1 T2 and T3 groups titan those in the C group. The differences between the Cr supplemented groups in this respect were not significant, however. Liver copper was higher (P < 0-001) in C while liver iron was the lowest (P < 0-05) in that group. The concentrations of copper, iron (P < 0-01) and zinc in the liver (P < 0-05) were higher after 35 than after 21 days while those of manganese were not affected by slaughter age. Protein accretion in the meat tended to be increased in the Cr supplemented groups while deposition of fat in the meat was lower but these differences were not significant (P > 0-05). Other meat quality parameters including sensory evaluation scores improved due to Cr supplementation and Cr-yeast complex was found to have exerted significantly greater effects on these parameters. It was concluded that the supplementation of 0-2mg Cr per kg food dry matter would improve the performance of broiler chickens in terms of metabolizability of organic nutrients, retention of trace elements and meat quality.

There is increasing interest in developing abattoir-based measures to assist in determining the welfare status of pigs. The primary aim of this study was to determine the most appropriate place on the slaughter line to conduct assessments of welfare-related lesions, namely apparent aggression-related skin lesions (hereafter referred to as ‘skin lesions’), loin bruising and apparent tail biting damage. The study also lent itself to an assessment of the prevalence of these lesions, and the extent to which they were linked with production variables. Finishing pigs processed at two abattoirs on the Island of Ireland (n=1950 in abattoir A, and n=1939 in abattoir B) were used. Data were collected over 6 days in each abattoir in July 2014. Lesion scoring took place at two points on the slaughter line: (1) at exsanguination (slaughter stage 1 (SS1)), and (2) following scalding and dehairing of carcasses (slaughter stage 2 (SS2)). At both points, each carcass was assigned a skin and tail lesion score ranging from 0 (lesion absent) to 3 or 4 (severe lesions), respectively. Loin bruising was recorded as present or absent. Differences in the percentage of pigs with observable lesions of each type were compared between SS1 and SS2 using McNemar/McNemar-Bowker tests. The associations between each lesion type, and both cold carcass weight and condemnations, were examined at batch level using Pearson’s correlations. Batch was defined as the group of animals with a particular farm identification code on a given day. The overall percentage of pigs with a visible skin lesion (i.e. score>0) decreased between SS1 and SS2 (P<0.001). However, the percentage of pigs with a severe skin lesion increased numerically from SS1 to SS2. The percentage of pigs with a visible tail lesion and with loin bruising also increased between SS1 and SS2 (P<0.001). There was a positive correlation between the percentage of carcasses that were partially condemned, and the percentage of pigs with skin lesions, tail lesions and loin bruising (P<0.05). In addition, as the batch-level frequency of each lesion type increased, average cold carcass weight decreased (P<0.001). These findings suggest that severe skin lesions, tail lesions and loin bruising are more visible on pig carcasses after they have been scalded and dehaired, and that this is when abattoir-based lesion scoring should take place. The high prevalence of all three lesion types, and the links with economically important production parameters, suggests that more research into identifying key risk factors is warranted.

The importance of the different adipose deposits with respect to the total dissectible fatty tissue in growing rabbits and lambs was studied. Development of the subcutaneous fat in the lamb is late and occurs after birth. In contrast, the internal types of fat, such as the perirenal and pelvic fat, represent at birth an important percentage of the total fat. They tend to lose part of their relative importance between 0 and 250 days after birth. In the rabbit the situation concerning the development of subcutaneous and perirenal fat is inverted. For both species the intermuscular fat remains almost constant in relative importance during the whole growth period.

After the period required for the establishment of the different fat deposits, we could observe in lambs, and to a smaller degree in rabbits, a relative growth which tends to become isometric with regard to the total fat deposits.

A total of 384 piglets from a three-way cross Yorkshire ♂ × (Finnish Landrace ♂ × Dutch Landrace♀) ♀ in the live-weight range of 11 to 25 kg were used in a feeding trial. The effect of supplementary microbial phytase from Aspergillus niger var. Van Tieghem on performance (average daily gain (ADG), average daily food intake (ADFI) and food conversion ratio (FCR)) and apparent digestibility of total phosphorus (P), in comparison with a non-supplemented basal diet or a diet supplemented with one or two levels of monocalcium phosphate (MCP), were studied.

The piglets were allotted to 12 blocks. Within each block, four experimental diets were offered ad libitum for a period of 4 weeks i.e. (1) basal diet; (2) basal diet + 1·0 g P, from MCP per kg diet; (3) basal diet + 1·9 g P, from MCP per kg diet; and (4) basal diet + 1450 phytase units (PTU) per kg diet. The diets, with added chromium III oxide as a marker, were cold pelleted without steam at a temperature not above 60°C to avoid a possible inactivation of the phytase. In all diets the ratio calcium: digestible P was intended to be constant.

Adding 1450 PTU of Aspergillus niger phytase enhanced the apparent digestibility of P by proportionately 0·216 when compared with diet 1 and by proportionately 0·100 compared with diet 3. In addition, significantly higher ADG and ADFI and better FCR were noted in pigs receiving supplementary microbial phytase than in pigs on any other treatment. The content of digestible P of the diet with added phytase, however, zvas between the two levels of the diets with added MCP.

A multivariate restricted maximum-likelihood procedure was used to estimate variance and covariance components between and within sires. This method, which considered all lactations simultaneously, accounted for the bias in later lactation records due to selection on dairy performance. Analysis was carried out for a mixed model with herd-year-seasons as fixed and sires as random effects, and fitting lactation length, calving age and month within season of calving as covariables. The data included 26 176 first, 19 978 second and 14 868 third lactation records for 679 test sires, and were analysed in 13 subsets. Additional records for proven sires, treated as fixed effects, were included to improve the data structure.

Estimates for all components were higher in later than in earlier data sets, probably, to a large extent, because of a scale effect. Pooled estimates of heritabilities for lactations 1 to 3 were 0·28, 0·19 and 0·24 for milk yield, and 0·27, 0·21 and 0·25 for fat yield. Genetic correlations of 0·91, 0·91 and 0·95 for milk yield, and 0·91, 0·91 and 0·99 for fat yield, were found between lactations 1 and 2, 1 and 3, and 2 and 3, respectively. Results suggest that dairy performance in all lactations is almost identical genetically.

Genetic lines for high and low liquid milk production were established within the British Friesian and British Jersey breeds by random matings of experimental dams to the ‘best’ and ‘worst’ nationally available progeny-tested sires. Some Friesian dams could also be classified as either high or low for genetic potential for milk yield on the basis of previous, but less rigorously controlled, matings to high-or low-production sires. The dams and their high and low potential-production female progeny were reared indoors, and given a single complete pelleted diet ad libitum from weaning until leaving the experiment after their third calving. Height at withers and width at hooks were recorded monthly, cumulate food intake and body weight fortnightly and milk yield, fat and protein concentration weekly, throughout life in the experiment. Measures of lactation production, food intake and efficiency of conversion of food to milk product during the whole calving-to-calving interval were obtained. Yields were about 0-8 times national average yields. Differences between high and low genetic lines appeared similar for the two breeds. High potential-production progeny produced more liquid milk, fat and protein but at a lower fat and protein concentration than did low-potential progeny. High-potential progeny consumed more food from calving to calving and had higher food conversion efficiencies to liquid milk, fat and protein. The response in efficiency accompanying a given change in production was close to that predicted by phenotypic regression of efficiency on yield with a 0-75% increase in efficiency for every 1% increase in yield. The likelihood of smaller returns in efficiency at higher yields is discussed. High potential-production progeny lost more body weight than did low during lactation, suggesting a greater withdrawal of energy from body reserves. High-potential progeny were neither heavier nor taller but were marginally narrower at the hooks than were low-potential progeny, suggesting the possibility of increased calving difficulties as selection for increased yield continues.

Friesians produced 50% more liquid milk, 13% more milk fat and 29% more milk protein and consumed 22% more food from calving to calving than did Jerseys. As a consequence Friesians were markedly more efficient at producing liquid milk (+23%) but essentially no more efficient at producing milk energy (+2%) than were Jerseys. In terms of both biological and commercial application of the results, any biases in the experiment probably operated against the Jersey relative to the Friesian.

Predictions of food intake using accepted feeding standards underestimated observed total food intake during lactation by a factor of 0-8. Linear regression indicated underestimation of maintenance requirement as a likely explanation.

In many cases health and welfare problems are observed in organic egg production systems, as are high environmental risks related to nutrient leaching. These disadvantages might be reduced if the layers are allowed to utilise their ability to forage to a higher degree thereby reducing the import of nutrients into the system and stimulating the hens to perform a natural behaviour. However, very little is known about the ability of modern high-producing layers to take advantage of foraging to cover their nutritional needs, and the aim of the present work was to clarify this subject. Six flocks, each of 26 hens and one cock, were moved regularly in a rotation between different forage crops for a period of 130 days. Half of the flocks were fed typical layer feed for organic layers and half were fed whole wheat. The forage crops consisted of grass/clover, pea/vetch/oats, lupin and quinoa. At the beginning of the experiment, wheat-fed hens had a lower intake of supplementary feed (wheat) and a lower laying rate, egg weight and body weight. However, after a period of 6 to 7 weeks, the intake of wheat increased to approximately 100 g per hen per day and the laying rate increased to the same level as for the hens fed layer feed. For both groups of hens egg weight and body weight increased during the remaining part of the experiment. Crop analysis revealed different food preferences for hens fed layer feed and wheat-fed hens. Wheat-fed hens ate less of the cultivated seeds, whereas the amounts of plant material, oyster shells, insoluble grit stone and soil were larger in the crops from wheat-fed hens. Floor eggs were significantly more frequent in the hens fed layer feed, whereas wheat-fed hens only rarely laid floor eggs. Irrespective of treatment, hens were found to have excellent health and welfare. We conclude that nutrient-restricted, high-producing organic layers are capable of finding and utilising considerable amounts of different feed items from a cultivated foraging area without negative effects on their health and welfare.

The aim of this study was to investigate the effect of dietary whole dried citrus pulp (DCP) on the antioxidant status of lamb tissues. In total, 17 lambs were divided into two groups and fed for 56 days: a barley-based concentrate diet (CON – eight animals), or a concentrate-based diet including 35% DCP to partially replace barley (CIT – nine animals). The CIT diet contained a double concentration of phenolic compounds than the CON diet (7.9 v. 4.0 g/kg dry matter (DM), respectively), but had no effect (P>0.05) on the overall antioxidant capacity of the hydrophilic fraction of blood plasma, liver and muscle. The CIT diet contained clearly more α-tocopherol than the CON diet (45.7 v. 10.3 mg/kg DM), which could explain the higher concentration of α-tocopherol in liver, plasma and muscle (P<0.05). The dietary treatment had no effect on the extent of lipid peroxidation, measured as thiobarbituric acid and reactive substances assay (TBARS values) in the faeces, small intestine, liver, plasma and muscle. Nevertheless, when muscle homogenates were incubated in the presence of Fe3+/ascorbate to induce lipid peroxidation, the muscle from lambs fed DCP displayed lower TBARS values (P<0.01), which negatively correlated with the concentration of α-tocopherol in muscle. These results showed that feeding whole DCP to ruminants increases the antioxidant status of muscle through an increase in the deposition of α-tocopherol.

Flaxseed is a rich source of α-linolenic acid and phytoestrogens, mainly lignans, whose metabolites (enterodiol and enterolactone) can affect estrogen functions. The present study evaluated the influence of dietary flaxseed supplementation on reproductive performance and egg characteristics (fatty acids, cholesterol, lignans and isoflavones) of 40 Hy-Line hens (20/group) fed for 23 weeks a control diet or the same diet supplemented with 10% of extruded flaxseed. The flaxseed diet had approximately three times the content of lignans (2608.54 ng/g) as the control diet, mainly secoisolariciresinol diglucoside (1534.24 v. 494.72 ng/g). When compared with the control group, hens fed flaxseed showed a similar deposition rate (72.0% v. 73.9%) and egg yield. Furthermore, there was no effect of flaxseed on the main chemical composition of the egg and on its cholesterol content. Estradiol was higher in the plasma of the control group (1419.00 v. 1077.01 pg/ml) probably due to the effect of flaxseed on phytoestrogen metabolites. The plasma lignans were higher in hens fed flaxseed, whereas isoflavones were lower, mainly due to the lower equol value (50.52 v. 71.01 ng/ml). A similar trend was shown in eggs: the flaxseed group had higher level of enterodiol and enterolactone, whereas the equol was lower (198.31 v. 142.02 ng/g yolk). Secoisolariciresinol was the main lignan in eggs of the flaxseed group and its concentration was three times higher then control eggs. Flaxseed also improved the n-3 long-chain polyunsaturated fatty acids of eggs (3.25 v. 0.92 mg/g egg), mainly DHA, however, its oxidative status (thiobarbituric reactive substances) was negatively affected. In conclusion, 10% dietary flaxseed did not affect the productive performance of hens or the yolk cholesterol concentration, whereas the lignans and n-3 polyunsaturated fatty acid content of eggs improved. Further details on the competition between the different dietary phytoestrogens and their metabolites (estrogen, equol, enterodiol and enterolactone) should be investigated.

The greenhouse gas (GHG) emissions, expressed in carbon dioxide equivalents (CO2-eq), of different Austrian biogas systems were analyzed and evaluated using life-cycle assessment (LCA) as part of a national project. Six commercial biogas plants were investigated and the analysis included the complete process chain: viz., the production and collection of substrates, the fermentation of the substrates in the biogas plant, the upgrading of biogas to biomethane (if applicable) and the use of the biogas or biomethane for heat and electricity or as transportation fuel. Furthermore, the LCA included the GHG emissions of construction, operation and dismantling of the major components involved in the process chain, as well as the use of by-products (e.g. fermentation residues used as fertilizers). All of the biogas systems reduced GHG emissions (in CO2-eq) compared with fossil reference systems. The potential for GHG reduction of the individual biogas systems varied between 60% and 100%. Type of feedstock and its reference use, agricultural practices, coverage of storage tanks for fermentation residues, methane leakage at the combined heat and power plant unit and the proportion of energy used as heat were identified as key factors influencing the GHG emissions of anaerobic digestion processes.

Estimates of genetic parameters for beef production traits were obtained for Piemontese cattle. Data were from 988 young bulls station-tested from 1989 till 1998. Bulls entered the station at 6 to 8 weeks of age and, after an adaptation period of 3 months, were tested for growth, live fleshiness and bone thinness. Length of test was 196 days. Growth traits considered were gain at farm, gain during the adaptation period, gain on test and total gain at the station. Six different fleshiness traits and bone thinness were scored on live animals at the end of the test using a linear system. Live evaluations of fleshiness were adjusted for the weight at scoring in order to provide an assessment of conformation independent of body size. Genetic parameters were estimated using animal models. Heritability of live-weight gain ranged from 0·20 in the adaptation period to 0·60 for total gain at the station. Genetic correlations between gains at station in different periods were high (from 0·63 to 0·97). Residual correlation between gain during the adaptation period and gain during test was negative, probably due to the occurrence of compensatory growth of the animals.

Live fleshiness traits and bone thinness were of moderate to high heritability (from 0·34 to 0·55) and highly correlated indicating that heavy muscled bulls also have thin bones. Accuracy of breeding values and therefore response to selection were improved by multiple trait analysis of the live fleshiness traits and bone thinness. Overall weight gain at the station had a moderate negative genetic correlation with all live fleshiness traits and bone thinness (from –0·11 to –0·39).

A 2×2×2 factorial experiment investigated the effect on lactational oestrus in sows of housing system (group or singly housed; G v. S), boar presence (boar present or absent; B v. N) and feeding level (ad libitum or restricted; A v. R). All treatments were imposed from day 20 post partum until weaning at day 42. A total of 183 Camborough hybrid female pigs were used, providing between 21 and 24 animals within each cell in the experimental design.

The occurrence of oestrus during lactation was observed as: 0·40 for G compared with 0·10 for S (P<0·01); 0·36 for B compared with 0·14 for N (P<0·05); and 0·28 for A compared with 0·21 for R (NS). The only treatment in which a high proportion of sows (0·78) showed oestrus was the combination G-B-A. Oestrus occurred during lactation on average 11 -4 days after imposing treatments, on average at day 20 post partum and 31·6 days after farrowing, and no significant differences were apparent between treatments. Individual piglet and overall litter weight gains were significantly reduced on G compared with S treatments (P < 0·01).

Sow food intake was greater for the S compared with G, for N compared with B, and A compared with R treatments (P<0·001). Sows on the R treatment lost significantly more live weight than the A sows between days 20 and 42 (P<0·001). Singly-housed sows also lost more weight than group-housed sows between farrowing and day 42 (P<0·001), mainly because of losses occurring before day 20.

Oxalic acid is found in high concentrations in some plants consumed by ruminants and may cause renal toxicity. To determine whether exposure to oxalic acid affects the capacity of the rumen of sheep and goats to degrade the compound, 20 animals (10 sheep and 10 goats) were dosed with free oxalic acid by gelatin capsule twice daily for 3 weeks at one of five levels (0·0, 0·3, 0·6, 0·9 and 1·2 mmol/kg live weight (M) per day). Rumen samples were collected by stomach tube in the week prior to the start of dosing and in each week of the 3-week experiment. Oxalic acid degradation capacity was measured by adding uC-labelled oxalic acid to rumen fluid in vitro and capturing evolved 14CO2. Rates of degradation increased with increasing level of administration (2·30, 4·71, 6·74, 9·83 and 13·90 mmol of oxalic acid degraded per I rumen fluid per day for doses 0·0, 0·3, 0·6, 0·9 and 1·2 mmol/kg M per day, respectively; P < 0·001). Rates of degradation increased during the dosing period (P < 0·001) with the largest increases occurring in the 1st week of dosing. Goats showed a greater response than sheep, with a higher mean oxalic acid degradation capacity (9·04 v. 5·95 mmol of oxalic acid degraded per I rumen fluid, P < 0·05). Oxalic acid administration did not influence plasma calcium concentration or cause renal function impairment as measured by plasma creatinine concentrations. The experiment demonstrated adaptation in the rumen to potential toxins in the host diet and suggests that the rumen micro-organisms of goats may have been more adapted to degrading oxalic acid than sheep.

A 3 × 2 factorial design experiment using 84 British Friesian cows was conducted to assess the effect of offering three types of grass silage and two levels of concentrate supplementation during early lactation on the total lactation performance of cows which calve during January and February. The three types of silage were early-cut wilted (ECW), early-cut unwilted (ECU) and late-cut unwilted (LCU), and the two levels of concentrate supplementation were 538 kg (LC) and 738 kg (HC) during a mean post-calving period of 71 days. The animals had a mean calving date of 29 January and were blocked according to calving date, and allocated at random to the treatments within each block on day 7 of lactation. The treatments were applied from day 7 of lactation until the animals were turned out to pasture on 18 April. Mean milk yields for the indoor period were: HC treatments 1963 (ECW), 1979 (ECU) and 1830 (LCU); LC treatments 1938 (ECW), 1942 (ECU) and 1731 (LCU) ± 41·3 kg; and for the total lactation were: HC treatments 5375 (ECW), 5438 (ECU) and 5284 (LCU); LC treatments 5509 (ECW), 5529 (ECU) and 5316 (LCU) ± 184·1 kg. There was no significant interaction between silage type and level of concentrate supplementation.

Milk composition, animal live weight and body condition were not significantly affected by either silage type or level of concentrate supplementation.