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Vitamin E is the major contributor to the antioxidant capacity in lambs fed whole dried citrus pulp

Published online by Cambridge University Press:  11 August 2016

G. Luciano ,
V. Roscini ,
S. Mattioli ,
S. Ruggeri ,
R. S. Gravador ,
A. Natalello ,
M. Lanza ,
A. De Angelis  and
A. Priolo
G. Luciano*
Affiliation:
Dipartimento di Scienze Agrarie Alimentari e Ambientali, Università degli Studi di Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy
V. Roscini
Affiliation:
Dipartimento di Scienze Agrarie Alimentari e Ambientali, Università degli Studi di Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy
S. Mattioli
Affiliation:
Dipartimento di Scienze Agrarie Alimentari e Ambientali, Università degli Studi di Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy
S. Ruggeri
Affiliation:
Dipartimento di Scienze Agrarie Alimentari e Ambientali, Università degli Studi di Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy
R. S. Gravador
Affiliation:
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Dublin, Ireland
A. Natalello
Affiliation:
Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, Via Valdisavoia 5, 95123, Catania, Italy
M. Lanza
Affiliation:
Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, Via Valdisavoia 5, 95123, Catania, Italy
A. De Angelis
Affiliation:
Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, Via Valdisavoia 5, 95123, Catania, Italy
A. Priolo
Affiliation:
Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, Via Valdisavoia 5, 95123, Catania, Italy
*
†E-mail: [email protected]
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Abstract

The aim of this study was to investigate the effect of dietary whole dried citrus pulp (DCP) on the antioxidant status of lamb tissues. In total, 17 lambs were divided into two groups and fed for 56 days: a barley-based concentrate diet (CON – eight animals), or a concentrate-based diet including 35% DCP to partially replace barley (CIT – nine animals). The CIT diet contained a double concentration of phenolic compounds than the CON diet (7.9 v. 4.0 g/kg dry matter (DM), respectively), but had no effect (P>0.05) on the overall antioxidant capacity of the hydrophilic fraction of blood plasma, liver and muscle. The CIT diet contained clearly more α-tocopherol than the CON diet (45.7 v. 10.3 mg/kg DM), which could explain the higher concentration of α-tocopherol in liver, plasma and muscle (P<0.05). The dietary treatment had no effect on the extent of lipid peroxidation, measured as thiobarbituric acid and reactive substances assay (TBARS values) in the faeces, small intestine, liver, plasma and muscle. Nevertheless, when muscle homogenates were incubated in the presence of Fe3+/ascorbate to induce lipid peroxidation, the muscle from lambs fed DCP displayed lower TBARS values (P<0.01), which negatively correlated with the concentration of α-tocopherol in muscle. These results showed that feeding whole DCP to ruminants increases the antioxidant status of muscle through an increase in the deposition of α-tocopherol.

Keywords

citrus pulpphenolic compoundsα-tocopherolruminantsantioxidant
Type
Research Article
Information
animal , Volume 11 , Issue 3 , March 2017 , pp. 411 - 417
Copyright
© The Animal Consortium 2016 

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References

Association of Official Analytical Chemists 1990. Official method of analysis, vol. 2, 15th edition. AOAC, Arlington, VA, USA.Google Scholar
Balasundram, N, Sundram, K and Samman, S 2006. Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence and potential uses. Food Chemistry 99, 191–203.Google Scholar
Bampidis, VA and Robinson, PH 2006. Citrus by-products as ruminant feeds: a review. Animal Feed Science and Technology 128, 175–217.Google Scholar
Bodas, R, Prieto, N, Jordán, MJ, López-Campos, O, Giráldez, FJ, Morán, L and Andrés, S 2012. The liver antioxidant status of fattening lambs is improved by naringin dietary supplementation at 0·15% rates but not meat quality. Animal 6, 863–870.Google Scholar
Burton, GW and Traber, MG 1990. Vitamin E: antioxidant activity, biokinetics and bioavailability. Annual Review of Nutrition 10, 357–382.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M and Stanley, GHS 1957. A simple method for the isolation and purification of lipids from animal tissue. Journal of Biological Chemistry 226, 497–509.Google Scholar
Fontanel, D 2013. Unsaponifiable matter in plant seed oils, 1st edition. Springer-Verlag, Berlin and Heidelberg, Germany.Google Scholar
García-Salas, P, Gómez-Caravaca, AM, Arráez-Román, D, Segura-Carretero, A, Guerra-Hernández, E, García-Villanova, B and Fernández-Gutiérrez, A 2013. Influence of technological processes on phenolic compounds, organic acids, furanic derivatives, and antioxidant activity of whole-lemon powder. Food Chemistry 141, 869–878.Google Scholar
Gladine, C, Morand, C, Rock, E, Bauchart, D and Durand, D 2007a. Plant extracts rich in polyphenols (PREP) are efficient antioxidants to prevent lipoperoxidation in plasma lipids from animals fed n-3 PUFA supplemented diets. Animal Feed Science and Technology 136, 281–296.Google Scholar
Gladine, C, Morand, C, Rock, E, Guffrat, D, Bauchart, D and Durand, D 2007b. The antioxidative effect of plant extracts rich in polyphenols differs between liver and muscle tissues in rats fed n-3 PUFA rich diets. Animal Feed Science and Technology 139, 257–272.Google Scholar
Gladine, C, Rock, E, Morand, C, Bauchart, D and Durand, D 2007c. Bioavailability and antioxidant capacity of plant extracts rich in polyphenols, given as a single acute dose, in sheep made highly susceptible to lipoperoxidation. British Journal of Nutrition 98, 691–701.Google Scholar
Gravador, RS, Jongberg, S, Andersen, ML, Luciano, G, Priolo, A and Lund, MN 2014. Dietary citrus pulp improves protein stability in lamb meat stored under aerobic conditions. Meat Science 97, 231–236.Google Scholar
Halliwell, B, Rafter, J and Jenner, A 2005. Health promotion by flavonoids, tocopherols, tocotrienols and other phenols: direct or indirect effects? Antioxidants or not? American Journal of Clinical Nutrition 81, 268–276.Google Scholar
Inserra, L, Priolo, A, Biondi, L, Lanza, M, Bognanno, M, Gravador, R and Luciano, G 2014. Dietary citrus pulp reduces lipid oxidation in lamb meat. Meat Science 96, 1489–1493.Google Scholar
Kerem, Z, Chertrit, D, Shoseyov, O and Regev-Shoshani, G 2006. Protection of lipids from oxidation by epicatechin, trans-resveratrol, and gallic and caffeic acids in intestinal model systems. Journal of Agricultural and Food Chemistry 54, 10288–10293.Google Scholar
Khan, MK, Huma, ZE and Dangles, O 2014. A comprehensive review on flavanones, the major citrus polyphenols. Journal of Food Composition and Analysis 33, 85–104.Google Scholar
Lanza, M, Scerra, M, Bognanno, M, Bognanno, M, Buccioni, A, Cilione, C, Biondi, L, Priolo, A and Luciano, G 2015. Fatty acid metabolism in lambs fed citrus pulp. Journal of Animal Science 93, 3179–3188.Google Scholar
Lindley, KJ, Goss-Sampson, MA, Muller, DPR and Milla, PJ 1994. Lipid peroxidation and electrogenic ion transport in the jejunum of the vitamin E deficient rat. Gut 35, 34–39.Google Scholar
López-Andrés, P, Luciano, G, Vasta, V, Gibson, TM, Biondi, L, Priolo, A and Mueller-Harvey, I 2013. Dietary quebracho tannins are not absorbed, but increase the antioxidant capacity of liver and plasma in sheep. British Journal of Nutrition 110, 632–639.CrossRefGoogle Scholar
López-Andrés, P, Luciano, G, Vasta, V, Gibson, TM, Scerra, M, Biondi, L, Priolo, A and Mueller-Harvey, I 2014. Antioxidant effect of ryegrass phenolics in lamb liver and plasma. Animal 8, 51–57.Google Scholar
Luciano, G, Vasta, V, Monahan, FJ, López-Andrés, P, Biondi, L, Lanza, M and Priolo, A 2011. Antioxidant status, colour stability and myoglobin resistance to oxidation of longissimus dorsi muscle from lambs fed a tannin-containing diet. Food Chemistry 124, 1036–1042.Google Scholar
Makkar, HPS, Blümmel, M, Borowy, NK and Becker, K 1993. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. Journal of the Science of Food and Agriculture 61, 161–165.Google Scholar
Monahan, FJ, Skibsted, LH and Andersen, ML 2005. Mechanism of oxymyoglobin oxidation in the presence of oxidizing lipids in bovine muscle. Journal of Agricultural and Food Chemistry 53, 5734–5738.CrossRefGoogle ScholarPubMed
Moñino, I, Martínez, C, Sotomayor, JA, Lafuente, A and Jordán, MJ 2008. Polyphenolic transmission to Segureño lamb meat from ewes’ diet supplemented with the distillate from rosemary (Rosmarinus officinalis) leaves. Journal of Agricultural and Food Chemistry 56, 3363–3367.Google Scholar
Mourão, JL, Pinheiro, VM, Prates, JAM, Bessa, RJB, Ferreira, LMA, Fontes, CMGA and Ponte, PIP 2008. Effect of dietary dehydrated pasture and citrus pulp on the performance and meat quality of broiler chickens. Poultry Science 87, 733–743.Google Scholar
Niki, E 2010. Assessment of antioxidant capacity in vitro and in vivo . Free Radical Biology and Medicine 49, 503–515.Google Scholar
Nogata, Y, Sakamoto, K, Shiratsuchi, H, Ishii, T, Iano, M and Ohto, H 2006. Flavonoid composition of fruit tissues of citrus species. Bioscience Biotechnology and Biochemistry 70, 178–192.Google Scholar
Pierre, F, Taché, S, Petit, CR, Van der Meer, R and Corpet, DE 2003. Meat and cancer: haemoglobin and haemin in a low-calcium diet promote colorectal carcinogenesis at the aberrant crypt stage in rats. Carcinogenesis 24, 1683–1690.Google Scholar
Sales, J and Koukolová, V 2011. Dietary vitamin E and lipid and colour stability of beef and pork: modeling of relationships. Journal of Animal Science 89, 2836–2848.CrossRefGoogle ScholarPubMed
Schüep, W and Rettenmaier, R 1994. Analysis of vitamin E homologous in plasma and tissue: high-performance liquid chromatography. Methods in Enzymology 234, 294–302.Google Scholar
Siu, GM and Draper, HH 1978. A survey of the malonaldehyde content of retail meats and fish. Journal of Food Science 43, 1147–1149.Google Scholar
Van Soest, PJ, Robertson, JB and Lewis, BA 1991. Methods for dietary fibre, neutral detergent fibre, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.Google Scholar
Zaspel, JB and Csallany, S 1983. Determination of alpha-tocopherol in tissue and plasma by high-performance liquid chromatography. Analytical Biochemistry 30, 146–150.Google Scholar