The biological activity against Boophilus microplus of extracts of the berries, bark, leaves and twigs of Pimenta dioica was examined and compared with that of eugenol, methyl eugenol and four commercial acaricides, namely dimethoate, farnesyl methyl ether, carbaryl and diazinon. The berry essential oil was more effective at inhibiting oviposition and causing mortality of the ticks than all the extracts, the commercial acaricides and methyl eugenol. At a dose of 1.0 mg/g it was 33, 12, 6, and 3 times as effective in inhibiting oviposition as the non-polar extracts of the leaves, bark, twigs and berries, respectively. The potency of the berry essential oil is attributable to eugenol, a phenyl propanoid derivative, which accounts for over 65% of the oil content.

Natural habitat degradation often involves the reduction or disappearance of bee species. In Africa, stingless bees are hunted for honey, which is used as food, for medicinal purposes, and for traditional rituals. Severe habitat degradation due to human settlement is hypothesized to have a negative impact on the species diversity of the African stingless bee species. In this paper, we assess the impact of habitat degradation on the diversity of five stingless bee species across different habitats in the tropical rainforest of Kenya (indigenous forest, mixed indigenous forest) and its neighbouring landscape (grassland, village) in western Kenya. The species fauna, nest occurrence, and species diversity of the stingless bee species varied across the different habitats. The number of nesting habitats of the meliponine species varied between habitats in the tropical rainforest. Meliponula ferruginea (reddish brown) nested in five habitats, while Meliponula bocandei and Meliponula ferruginea (black) nested only in two habitat types. The species richness decreased within the different types of habitats and the indigenous and mixed indigenous forest contained more species than other habitats. The fauna composition in both homesteads was exclusively similar, while the indigenous and mixed indigenous forests were mostly similar. Similarity in habitat preferences for nesting was revealed between M. bocandei vs Plebeina hildebrandti and M. ferruginea (reddish brown) vs Hypotrigona gribodoi. The natural native indigenous forest had the most diverse community compared to the degraded habitats. There are taxon-specific responses to habitat change; and in our study, there is clear value in conserving the native indigenous forest.

The striped mealybug, Ferrista virgata (Ckll.) has become a very serious pest of guava in India. Since the insecticides are ineffective, an attempt was made to suppress F. virgata with an introduced coccinellid, Cryptolaemus montrouzieri Mulsant C. montrouzieri and other local natural enemies especially Aenasius advena Comp. and Scymnus coccivora Ayyar effectively controlled F. virgata in guava orchards.

Two isolates of nucleopolyhedroviruses (NPVs) from Kenya and South Africa were compared to Gemstar® (a commercial NPV) for their pathogenicity against the first four larval instars of Helicoverpa armigera (Hübner). The larvae were fed on droplets with the three virus products in concentrations of 0 (control), 6×102, 6×103, 6×104 and 6×105 occlusion bodies/μl. The bioassays showed that the median lethal dose (LD50) values of 23 and 631 occlusion bodies for the first and second instars, respectively, were comparable to those of Gemstar®. The LD50 values for the third and fourth instars were 3981 and 39,810 for the Kenyan isolate and 1288 and 25,119 for the South African isolate. There was a linear relationship between the log LD50, the larval age and the lethal time (LT50), which appeared to be dose dependent. This correlation constitutes a useful index for estimating susceptibility of larval populations. The LT50 increased from 2.8 to 11.9 days and 2.8 to 6.8 days, respectively, for the Kenyan and South African isolates, suggesting a slight increase of resistance with age within infected larvae.

The salivary glands of the cowpea pod-sucking bugs Anoplocnemis curvipes (Fabricius), Clavigralla tomentosicollis Stål, Clavigralla shadabi Dolling, Riptortus dentipes (Fabricius) and Mirperus jaculus (Thunberg) are described and illustrated. Extracts of the glands were assayed for the presence of proteinases, α-amylase, β-amylase and amyloglucosidase. The salivary glands consist of four-lobed principal glands and a tubular accessory gland, and they differ in shape and size among the different species except for the median lobe, which is more or less spherical. The sickle-shaped nature of the posterior lobe reported before for Riptortus linearis (Linnaeus) was also observed in R. dentipes, suggestive of a probable generic characteristic. Anoplocnemis curvipes has the biggest salivary gland, and it is followed by R. dentipes, M. jaculus, C. tomentosicollis and C. shadabi in descending order. Assays of the gland extracts revealed that the four digestive enzymes tested are present in the five coreoid species. Proteinases were preponderant (1.4–94.9 μg/ml), while amylases were detected in traces [(0.5–11.0) × 10− 3 μg/ml]. Proteinases may be largely responsible for the feeding damage caused by the coreoid species and their concentration in the glands of the different species correlates well with their known capacity to inflict pod damage and yield reduction in cowpea. Fourth instar nymphs of the different species also had higher concentrations of the digestive enzymes compared with their respective adults; and this correlates with the known ability of the nymphs to cause greater pod damage and yield reduction than adults.

Bemisia tabaci Gennadius is a major pest on cotton and vegetable plants in Africa. It is considered as a cryptic species complex. Identification of the most damaging species such as the Middle East–Asia Minor 1 (B biotype) and Mediterranean (MED) (which contains the Q and Africa silverleaf (ASL) biotypes) species represents an important step towards the management of B. tabaci. Some data on the geographical distribution of the B. tabaci species complex exist in Burkina Faso, Benin and Togo, but data on the pest's invasion and displacement dynamics, in relation to time, are lacking. Here, molecular markers (mitochondrial cytochrome oxidase, mtCO1) were used to determine the identity of B. tabaci. Our results illustrate population dynamics on cotton and vegetable plants between 2007, 2009 and 2010. On cotton in southern Togo, ASL was predominant and found in sympatry with Q1. Its frequency decreased slightly over time, i.e. from 92% in 2009 to 90% in 2010. In Burkina Faso, Q1, Q3 and ASL biotypes showed different temporal and spatial distribution patterns. There, Q1 dominated on cotton plants throughout the study. This work provides relevant information about the population dynamics of B. tabaci MED species in two West African countries, Burkina Faso and Togo, in connection with pest management programmes.

Insect-free tubers of four potato varieties, namely Kenya Baraka, Anett, Kerrs Pink and Roslin Gucha were exposed to adult Phlhorimaea operculella for 14 days in uncontrolled conditions in storages. On examination of the tubers 16 days after exposure, the percentage of infested tubers was determined: Kenya Baraka—62.50, Anett—77.50, Kerrs Pink—85.00 and Roslin Gucha—86.25%. The larvae made the following mean tunnel lengths: 44.65, 95.33, 88.33 and 104.23 cm in Kenya Baraka, Anett, Kerrs Pink and Roslin Gucha varieties, respectively. Although there were no statistically significant differences in the number of tubers infested (per cent), there were significant differences in the mean length of tunnels made by the larvae in Kenya Baraka and the other three varieties at P < 0.05. The mean number of larvae which survived in 10 tubers were 6.75, 11.25, 15.75 and 18.00 for Kenya Baraka, Anett, Kerrs Pink and Roslin Gucha varieties, respectively. There were statistically significant differences between Kenya Baraka and Kerrs Pink; Kenya Baraka and Roslin Gucha; and Roslin Gucha and Anett varieties (P < 0.05). Thus Roslin Gucha was the least resistant of the four varieties tested, whereas Kenya Baraka emerged as the most resistant variety. The mechanism of resistance in this case was most probably antibiosis.

The species diversity of tephritid fruit flies in major mango-growing regions in Uganda was monitored over a 2-year period (2010–2012) using fruit bait and lure traps. A total of 368,332 specimens belonging to 10 species in four genera (Bactrocera, Ceratitis, Trirhithrum and Dacus) were collected. Of these, 98.9% belonged to Bactrocera invadens, while the second and third most common species were Dacus bivittatus (0.4%) and Ceratitis anonae (0.3%), respectively. Significant differences in the evenness and diversity of fruit fly species were noted across the regions. Fruit fly community structure was significantly different across the three regions. The Lake Victoria Crescent and Mbale Farmlands harboured significantly more D. ciliatus, T. coffeae, D. bivittatus and B. cucurbitae in contrast to the Northern Moist Farmlands and the Western Medium High Farmlands. Ceratitis rosa contributed the highest difference in regional structure, followed by C. fasciventris and C. cosyra. Rank abundance curves depicted a geometric series distribution of the species composition in the three regions, confirming a scenario of competitive displacement of native fruit fly species by B. invadens. A comprehensive and sustainable response strategy to B. invadens and other fruit flies needs to be urgently devised to protect the horticulture industry in Uganda.

Vegetable and grain amaranths represent a vital source of micronutrients and protein in Asia and Africa. However, various foliar lepidopteran pests and stem-mining weevils hinder amaranth production. Insect-resistant cultivars can enhance the productivity of this crop. Here, we report on the performances of amaranth varieties screened for their resistance to insect pests under the field conditions at The World Vegetable Center stations in Asia and sub-Saharan Africa. We conducted two preliminary screening trials with a total of 263 entries from around the world in Taiwan and a third preliminary screening trial with 49 African-indigenous entries in Tanzania. Promising entries from these preliminary trials were collectively evaluated in an advanced screening trial in Tanzania, to identify lines resistant to foliar and stem-boring pests in East Africa. Four entries exhibited moderate resistance to foliar pests: TZ51 and TZ53 (Amaranthus cruentus), TZ34 (A. dubius) and TZ39 (Amaranthus sp.). Five entries showed moderate resistance to stem weevils: TZ06 and TZ27 (A. cruentus), TZ52 (A. graecizans), TZ59 (A. palmeri) and TZ07 (Amaranthus sp.). Lepidopteran pests affecting leaves were reared to adulthood and identified as Spoladea recurvalis (Crambidae), Spodoptera exigua (Noctuidae) and Spodoptera littoralis (Noctuidae). Stem weevil larvae were also reared and identified as: Neocleonus sannio Herbst, Gasteroclisus pr. rhomboidalis Boheman, Hypolixus pr. haerens Boheman and Baradine sp. (Curculionidae). These results highlight key amaranth pests in East Africa and identify insect-resistant entries that will be useful in breeding programmes and resistance studies.

Lepidopteran stemborers (Chilo zacconius Bleszynski, Chilo diffusilineus de Joannis and Maliarpha separatella Ragonot) and diopsid flies (Diopsis spp.) are economically significant insect pests of rice in Burkina Faso. The objective of this study was to monitor damage trends and the pre-imaginal populations of these insects on two host plants, cultivated (Oryza sativa L.) and wild rice (Oryza longistaminata Chev and Roehr). The study was conducted over four consecutive rice crop seasons from 1999 to 2001 in three irrigated rice-growing locations in western Burkina Faso. Sampling of cultivated rice was conducted monthly in 1 m2 quadrates in 100 randomly selected farmers' fields. Data on wild rice were collected monthly from each of the three locations using 500 tillers that were plucked at random and dissected. Results showed that in the absence of a rice crop lepidopteran stemborers and diopsid flies are able to survive on O. longistaminata, which can host the pre-imaginal populations of these insects throughout the year. Damage caused on cultivated rice varied across farmers' fields, seasons and phenological stages of the plants. With 8% the highest average extent of ‘whiteheads’ was recorded during the dry season. These results are important elements in the design of a sustainable management strategy for these insect pests.

Morphology of haemocytes of healthy and Beauveria bassiana (Bals.) Vuill.-infected Dicladispa armigera (Olivier) adults was examined in a phase contrast microscope to characterize them into different types. The total haemocyte count (THC) and the differential haemocyte count (DHC) were also estimated. Four types of haemocytes were identified: prohaemocyte, plasmatocyte, granulocyte and spherulocyte. In the healthy adults, the THC varied between 5055 and 5950/mm3, out of which 85% were composed of granulocytes and plasmatocytes. As B. bassiana infection time increased, THC decreased and was associated with a significant concomitant increase in granulocytes and a decrease in prohaemocytes. Granulocytes reacted in various ways, including disintegration of the plasma membrane, formation of fine pseudopod-like cytoplasmic extensions and finally, clumping of cells. Granulocytes and plasmatocytes were the predominant haemocytes involved in all the cell-mediated defence reactions.

The attraction of the melon fly, Bactrocera cucurbitae (Coq.) (Diptera: Tephritidae) to soybean hydrolysate, fishmeal, beef extract, banana/grapes, bread and dog biscuit was evaluated in snakegourd (Trichosanthes anguina L.) gardens during 2000–2001. Vinegar and beer were added as the ‘bait components’ to the above ‘base baits’ to enhance their attractiveness. Edible oils, glycerine and petroleum jelly were tested as the ‘controlled releasers’ to sustain the attractiveness. The results indicated that banana and soybean hydrolysate were 85–95% more attractive to adult B. cucurbitae than fishmeal, beef extract, bread and dog biscuit. Among the fruit pulps, grapes and banana appeared to be more attractive than pineapple. The attractiveness of baits with palm oil lasted longer (up to 5 days) than that of baits without any controlled releaser (2–3 days). Grapes + beer + palm oil was found to be 37% more attractive than the other admixtures. The fruit flies were attracted towards the baits more intensively between 0600 and 0800 h and between 1600 and 1800 h.

Three species of fruit fly parasitoids, Psyttalia concolor (Szépligeti), Psyttalia cosyrae (Wilkinson) and Psyttalia lounsburyi (Silvestri) (Hymenoptera: Braconidae) were reared on different host larvae to determine the effects of host species, size and colour on parasitoid development. Ovipositor, ovipositor sheath and hind tibia lengths were found to be different for progenies from preferred and non-preferred host larvae. Ratios of ovipositor–tibia and ovipositor sheath–tibia showed no difference when P. concolor was reared on a bigger host (Ceratitis cosyra) (Walker) (Diptera: Tephritidae), whereas rearing P. cosyrae on a smaller host (C. capitata) (Wiedemann) showed significant differences. Comparison of the linear measurements, ratios and number of flagellomeres of parasitoids reared on preferred hosts, separated the species into their natural groupings. When reared on a different-coloured host, P. lounsburyi lost its dark markings but other characters remained unchanged. Morphometric analysis also indicated differences between parasitoids reared on preferred host larvae and those reared on non-preferred host larvae. Rearing parasitoids on larvae other than their preferred hosts resulted in colour and size changes, and thus, these characters alone were not reliable for the identification of parasitoids. This is especially important in post-release sampling surveys to ascertain establishment of the parasitoids in new environments where they may adapt to new host species.

Tick populations were monitored between October 1991 and September 1992 at monthly intervals on 10 Boran (Bos indicus) heifers at Didtuyura ranch, Yabelo district, Ethiopia. Twelve different tick species in four genera were identified from a total of 16 026 specimens collected. Of all the ticks collected, Rhipicephalus spp. constituted 84.7%, Amblyomma spp. 9.9%, Boophilus spp. 4.8% and Hyalomma spp. 0.24%. The ventral parts of cattle were found to be the most favoured feeding sites for most of the ticks collected. The size ranges of the standard ticks collected were 4–8 mm; 9–16 mm; 4–8 mm and 8–13 mm for R. pulchellus, A. variegatum, B. decoloratus and A. gemma, respectively, while their respective male to female sex ratios were 2:1; 3.2:1; 1:5.4 and 2.6:1. Packed cell volume (PCV) values of blood samples taken from 600 cattle were monitored monthly during the study period. There was a reduction in PCV during the dry season of the year but generally the values were within the normal range of 32–40%. The parasites causing anaplasmosis, babesiosis and theileriosis (T. mutans) were detected by microscopic examination of blood smears and serologically (ELISA) at seroprevalence rates of 94.68%, 77.19% and 30.9% respectively.

Phenolic tsetse attractants in host urine were shown to form gradually from proattractants identified as a mixture of glucuronates and sulphates. The breakdown of proattractants is predominantly due to microbial activity. Of the eight bacteria isolated from buffalo urine three have demonstrated varying ability to give rise to free phenols the formation of which was monitored by observing the build-up of 4-cresol by means of gas chromatography. These observations may provide a useful biotechnological model for controlled release of these semiochemicals in the field.

This study evaluated the potential use of commercial mycoinsecticide formulations against red-gum lerp psyllid Glycaspis brimblecombei Moore in semi-field conditions. Eucalypt seedlings infested with psyllid nymphs were sprayed with different formulations and two concentrations of each product. Conidial deposits were evaluated after spraying for control efficiency. The conidial deposit was affected by the pathogen species and by formulation types. Higher conidial deposits were associated with mycoinsecticide formulation concentrates of lower granulometry and oil dispersion. However, some products with low deposits of conidia were highly efficient against psyllid nymphs. The results showed that the use of entomopathogenic fungi is a promising alternative method for controlling the red-gum lerp psyllid.

Cotesia vestalis was introduced into Kenya from South Africa in March 2003 for biological control of Plutella xylostella with initial release in 2004. To confirm establishment, the rate of spread beyond the release sites and parasitism rates by C. vestalis were assessed between October 2011 and April 2012 in eastern and southeastern Kenya (Kitui, Mwingi/Yatta and Loitokitok areas). Monthly observations on kale infestation by diamondback moth, damage scores, parasitism and parasitoid guild were undertaken on 20 randomly selected plants in farmer-managed kale farms. Sampled larval and pupal stages were placed in containers and taken to the laboratory for parasitoid emergence. The infestation levels ranged from 0.4 to 2.5 DBM per plant, while damage scores ranged from 1.2 to 1.6, which correlated with the infestation levels. Cotesia vestalis was not only the most abundant parasitoid, but it had also spread to new areas from the release sites. In December, the three sites recorded an average of 50% from C. vestalis. Excluding the exotic parasitoids C. vestalis and D. semiclausum, indigenous parasitoids accounted for less than 5% total parasitism. The parasitoid guild of the diamondback moth varied between months and study sites with January recording the highest species diversity and evenness of 1.15 and 0.63, 1.28 and 0.80, 1.08 and 0.47 in Loitokitok, Kitui and Mwingi/Matuu, respectively. Therefore, C. vestalis had an impact on the management of the DBM with parasitism rate of 35% to 88% and had spread beyond the release sites.

Genetic and biological implications from interbreeding the Ghanaian okra and cassava biotypes of Bemisia tabaci (Gennadius), reared on eggplant, were studied in reciprocal crossing plans and crosses compared with parental generations using RAPD–PCR. Interbreeding did not affect fecundity of mated females and survival of F1 progeny. However, the sex ratio of the hybrid progeny was significantly male dominated (by 70% males) compared to purebred progeny of each biotype (>60% females) (P<0.01). Each biotype was characterized by its own RAPD profiles. Hybrid females had one locus identical to that of both parents, while the males resembled the mother biotype at two loci. The F1 females were oviposited normally and there was a possible restricted gene flow between insects from the two biotypes, sharing the same host plant. However, the biological isolation seems to buttress ecological isolation in nature, hence maintaining the genetic and biological identity of sympatric populations in both biotypes.

Surveys of the cereal stemborers and their natural enemies in Zambia were initiated in 1997. The surveys verified the presence of three stemborer species on maize, sorghum and pearl millet. These were the maize stalk borer, Busseola fusca Fuller (Lepidoptera: Noctuidae), the spotted stemborer, Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) and the African pink borer, Sesamia calamistis Hampson (Lepidoptera: Noctuidae). Occasionally, the sugarcane borer, Eldana saccharina Walker (Lepidoptera: Pyralidae) was also recovered especially around areas near the sugarcane plantation at Mazabuka, Southern province. Chilo partellus was the predominant stemborer in region I (areas with less than 600 mm of rainfall per annum). Three ‘hot spot’ areas, namely Luangwa, Buleya Malima and Kalobolelwa, were identified based on the abundance of Ch. partellus. The exotic parasitoid, Cotesia flavipes Cameron (Hymenoptera: Braconidae) was released in the three areas for the control of Ch. partellus. However, post-release surveys in these areas have not revealed any recoveries of Co. flavipes. Three indigenous natural enemies, namely Cotesia sp., Dentichasmias sp. and Dolichogenidea sp. emerged from the field-collected larvae.

Despite the continuous use of synthetic insecticides during the last two decades, the tea mosquito bug Helopeltis theivora Waterhouse still exists as the most destructive pest of tea in North East India. The susceptibility levels of the female sucking bug collected from conventional (synthetic insecticide treated: Terai and Dooars plain regions) and organic (synthetic insecticide untreated: low-altitude Darjeeling region) tea plantations of the northern part of West Bengal to two synthetic insecticides, quinalphos and cypermethrin, and the activity of three principal detoxifying enzymes were assayed. Compared with the susceptible Darjeeling population, the Terai and Dooars populations showed a resistance factor at the lethal concentrations for 50% level ranging from 547- to 2680.87-fold and from 3810- to 7480-fold for quinalphos and cypermethrin, respectively. General esterases (GEs), glutathione S-transferases (GSTs) and cytochrome P450-mediated mono-oxygenases (CYPs) also showed an increased activity in the Terai and Dooars populations compared with those from Darjeeling. Defence enzyme activity was enhanced by 15.4- and 17.6-fold for GEs, 1.8- and 1.9-fold for GSTs and 2.1- and 2.4-fold for CYPs in the synthetic insecticide-treated H. theivora populations when compared with the untreated Darjeeling populations. Electrophoretic analysis for GEs showed a higher level of expression for esterase I–VI isozymes in the Terai and Dooars populations when compared with that in the Darjeeling populations. This study reveals a reduced efficacy of quinalphos and cypermethrin against field populations of H. theivora, possibly due to enhanced activities of GEs, GSTs and CYPs. The findings may be used in developing integrated resistance management strategies that can help in the effective control of this major tea pest.