Herbicides that inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD) can be mixed with herbicides that generate reactive oxygen species (ROS) to enhance the spectrum, level, speed, and consistency of weed control efficacy; however, mixtures of these herbicides can increase corn injury. A total of five field trials were conducted from 2021 to 2023 in Ridgetown, Ontario, to determine the sensitivity of two corn hybrids (‘DKC39-97’ and ‘B79N56PWE’) to tolpyralate plus ROS-generating herbicides (atrazine, bromoxynil, bentazon, or glufosinate) applied postemergence at the recommended rate (1×) and sequentially to represent a spray overlap (2×) in the field. Tolpyralate plus atrazine, bromoxynil, bentazon, or glufosinate (2× rates) caused greater corn injury to DKC39-97 than B79N56PWE corn at 1, 2, and 4 wk after treatment (WAT). Tolpyralate plus atrazine, bromoxynil, bentazon, or glufosinate (2× rates) caused 38%, 36%, 29%, and 18% injury to DKC39-97 corn, but only 5%, 20%, 9%, and 2% injury to B79N56PWE corn, respectively at 1 WAT. Corn injury to both hybrids decreased over time with ≤2% injury at 8 WAT. Tolpyralate + atrazine, bromoxynil, or bentazon (2× rates) caused a 17%, 16%, and 13% height reduction, respectively, of DKC39-97 corn at 2 WAT; however, tolpyralate + glufosinate did not reduce DKC39-97 corn height. Tolpyralate + bromoxynil or bentazon (2× rates) caused a 12% and 10% height reduction of B79N56PWE corn, respectively, at 2 WAT; however, tolpyralate + atrazine or glufosinate did not reduce B79N56PWE corn height. Tolpyralate + atrazine or glufosinate (2× rates) caused a greater corn height reduction of DKC39-97 corn than B79N56PWE corn at 2 WAT. Grain yield was on average 2% lower from DKC39-97 than B79N56PWE corn. Tolpyralate + bromoxynil or bentazon (2× rates) caused 7% and 6% corn grain yield reduction compared to tolpyralate plus glufosinate (2× rate). Results indicate that tolpyralate + ROS-generating herbicides can cause corn injury, which is influenced by corn hybrid and ROS-generating herbicide. Corn producers need to consider the differential sensitivity of corn hybrids and ROS-generating herbicides when using an HPPD-inhibiting herbicide for weed management.

Tiafenacil is registered in the United States for use in annual crops such as corn and soybean, but not on orchard crops. Field studies were conducted to determine orchard crop safety and efficacy of tiafenacil on important California orchard weeds. To evaluate crop safety, tiafenacil was applied at 74, 148, and 222 g ai ha−1 alone and with 38 g ai ha−1 of tolpyralate three times per year at the base of almond, pistachio, prune, and walnut trees. The first treatment was applied 2 mo after the trees had been transplanted. In all four tree crop experiments, treatments were applied once in May 2020, then three times again during the winter of 2021 and 2022 at 21-d treatment intervals. There were no visual foliar injury symptoms or treatment-related effects on tree trunk diameter change even at the highest tested rate of tiafenacil applied seven times over three growing seasons. In a separate study of weed control, in most instances, tiafenacil applied at 12 g ai ha−1 performed similarly to that of tiafenacil plus glufosinate. Control of glyphosate-resistant hairy fleabane with tiafenacil applied alone at 25 g ai ha−1 was 65% by 14 d after treatment. Tiafenacil applied at 50 g ai ha−1 to hairy fleabane performed similarly to glufosinate plus glyphosate. In a greenhouse study, tiafenacil applied at 12 g ha−1 provided 95% to 100% control of barnyardgrass and junglerice, and there was no significant difference between tiafenacil applied alone or with glufosinate. Saflufenacil applied alone or in a mixture with glufosinate was not as effective as the tiafenacil treatments for grass weed control. Based on experiments conducted over three growing seasons in four tree fruit and tree nut crops, tiafenacil crop safety appeared to be acceptable even at up to 2- or 3-fold the expected use rate.

Pink purslane is often ranked as one of the most troublesome weeds in vegetable production systems in Georgia. Pink purslane encroachment along field edges and in-field of agronomic crops has recently increased. Postemergence herbicides are an effective component of agronomic crop weed management. However, little research has addressed pink purslane control in agronomic crops. Therefore, greenhouse and field studies were conducted from 2022 to 2023 in Tifton, Georgia, to evaluate the response of pink purslane to postemergence herbicides commonly used in agronomic crops. Greenhouse screening provided preliminary evidence whereby 13 of the 21 postemergence herbicides evaluated provided ≥80% aboveground biomass reductions. These 13 herbicides were then used for field studies. Results from the field studies, pooled across two locations, indicated that only three of the 13 herbicides provided aboveground biomass reductions of ≥70% compared to the nontreated control. Those herbicides included atrazine at 1,682 g ai ha−1, glufosinate at 656 g ai ha−1, and lactofen at 219 g ai ha−1 with 79%, 70%, and 83% biomass reduction, respectively (P < 0.05). This research suggests that many of the postemergence herbicides used on agronomic crops will not effectively control pink purslane. Thus, when trying to manage pink purslane with postemergence herbicides in agronomic crops, growers should plant crops or cultivars that are tolerant of either atrazine, glufosinate, lactofen, or a combination of these.

Six field experiments were established in southwestern Ontario in 2021 and 2022 to evaluate whether the addition of a grass herbicide (acetochlor, dimethenamid-p, flufenacet, pendimethalin, pyroxasulfone, or S-metolachlor) to tolpyralate + atrazine improves late-season weed control in corn. Tolpyralate + atrazine caused 12% and 5% corn injury at 1 and 4 wk after herbicide application (WAA); corn injury was not increased with the addition of a grass herbicide. Weed interference reduced corn yield 60%. The addition of a grass herbicide to tolpyralate + atrazine did not enhance velvetleaf control. The addition of acetochlor or dimethenamid-p to tolpyralate + atrazine enhanced pigweed species control 4% 4 WAA; the addition of other grass herbicides tested did not increase pigweed species control. The addition of acetochlor enhanced common ragweed control 5% at 4 WAA, and the addition of acetochlor or dimethenamid-p enhanced common ragweed control 8% at 8 WAA; the addition of other grass herbicides did not improve common ragweed control. The addition of acetochlor to tolpyralate + atrazine enhanced common lambsquarters control up to 4%; there was no enhancement in common lambsquarters control with the addition of the other grass herbicides. Tolpyralate + atrazine controlled barnyardgrass 90% and 78% at 4 and 8 WAA, respectively; the addition of a grass herbicide enhanced barnyardgrass control 9% to 10% and 21% at 4 and 8 WAA, respectively. Tolpyralate + atrazine controlled green or giant foxtail 80% and 69% at 4 and 8 WAA, respectively; the addition of a grass herbicide enhanced foxtail species control 15% to 19% and 24% to 29% at 4 and 8 WAA, respectively. This research shows that adding a grass herbicide to tolpyralate + atrazine mixture can improve weed control efficacy, especially increased annual grass control in corn production.

The herbicides that inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD) are primarily used for weed control in corn, barley, oat, rice, sorghum, sugarcane, and wheat production fields in the United States. The objectives of this review were to summarize 1) the history of HPPD-inhibitor herbicides and their use in the United States; 2) HPPD-inhibitor resistant weeds, their mechanism of resistance, and management; 3) interaction of HPPD-inhibitor herbicides with other herbicides; and 4) the future of HPPD-inhibitor-resistant crops. As of 2022, three broadleaf weeds (Palmer amaranth, waterhemp, and wild radish) have evolved resistance to the HPPD inhibitor. The predominance of metabolic resistance to HPPD inhibitor was found in aforementioned three weed species. Management of HPPD-inhibitor-resistant weeds can be accomplished using alternate herbicides such as glyphosate, glufosinate, 2,4-D, or dicamba; however, metabolic resistance poses a serious challenge, because the weeds may be cross-resistant to other herbicide sites of action, leading to limited herbicide options. An HPPD-inhibitor herbicide is commonly applied with a photosystem II (PS II) inhibitor to increase efficacy and weed control spectrum. The synergism with an HPPD inhibitor arises from depletion of plastoquinones, which allows increased binding of a PS II inhibitor to the D1 protein. New HPPD inhibitors from the azole carboxamides class are in development and expected to be available in the near future. HPPD-inhibitor-resistant crops have been developed through overexpression of a resistant bacterial HPPD enzyme in plants and the overexpression of transgenes for HPPD and a microbial gene that enhances the production of the HPPD substrate. Isoxaflutole-resistant soybean is commercially available, and it is expected that soybean resistant to other HPPD inhibitor herbicides such as mesotrione, stacked with resistance to other herbicides, will be available in the near future.

Tolpyralate is an herbicide that is usually mixed with atrazine for broad-spectrum weed control in corn. Previous research has provided information on the effective dose (ED) of tolpyralate applied alone and in a 1:33.3 mixture with atrazine; however, tolpyralate is commercially applied at a dose of 30 to 40 g ai ha−1 with a minimum of 560 g ai ha−1 of atrazine. Therefore, five field trials were conducted over 3 yr (2019 to 2021) to determine the ED of atrazine to complement 30 g ai ha−1 of tolpyralate to achieve 80%, 90%, and 95% control of seven weed species 2, 4, and 8 wk after application (WAA). Tolpyralate was applied alone and in a mixture with atrazine doses ranging from 50 to 2,000 g ai ha−1. At 8 WAA, the ED of atrazine for 95% control of velvetleaf, common ragweed, common lambsquarters, and wild mustard was below the minimum label dose of atrazine on the commercial tolpyralate label, ranging from 430 to 520 g ai ha−1, which supports the use of the minimum label dose of atrazine. In contrast, redroot pigweed required 1,231 g ai ha−1 of atrazine to complement tolpyralate for 95% control 8 WAA. At 8 WAA, barnyardgrass and a mixture of green foxtail and giant foxtail (Setaria spp.) were not controlled by 80%, 90%, or 95% with tolpyralate applied alone or co-applied with any dose of atrazine evaluated in this study. The results of this study conclude that tolpyralate + atrazine is highly efficacious on several weed species at atrazine doses of 40 to 130 g ai ha−1 below the label dose of 560 g ai ha−1, but the use of the higher dose of tolpyralate or another herbicide may be required to improve control of redroot pigweed and grass weed species.

The complementary modes of action of 4-hydroxyphenylpyruvate dioxygenase (HPPD) and photosystem II (PSII) inhibitors have been credited for the synergistic weed control improvement of several species. Recent research discovered that reactive oxygen species (ROS) generation and subsequent lipid peroxidation is the cause of cell death by the glutamine synthetase inhibitor glufosinate. Therefore, a basis for synergy exists between glufosinate and HPPD inhibitors, but the interaction has not been well reported. Four field experiments were conducted in Ontario, Canada, in 2020 and 2021 to determine the interaction between HPPD-inhibiting (mesotrione and tolpyralate) and ROS-generating (atrazine, bromoxynil, bentazon, and glufosinate) herbicides on control of annual weed species in corn (Zea mays L.). The ROS generators were synergistic with the HPPD inhibitors and provided ≥95% control of velvetleaf (Abutilon theophrasti Medik.), except for tolpyralate + glufosinate, which was additive at 8 wk after application (WAA) and provided 87% control. Tank mixes of HPPD inhibitors plus ROS generators were synergistic for the control of common ragweed (Ambrosia artemisiifolia L.), except for tolpyralate + glufosinate, which was antagonistic at 8 WAA. Tolpyralate + glufosinate was antagonistic for the control of barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] and Setaria spp. at 8 WAA. Common lambsquarters (Chenopodium album L.) control at 8 WAA was synergistic and ≥95% with mesotrione plus atrazine, bromoxynil, or glufosinate and with tolpyralate plus bromoxynil or bentazon. Herbicide tank mixes were generally additive for the control of wild mustard (Sinapis arvensis L.) at 8 WAA, except for the synergistic tank mixes of tolpyralate plus atrazine or bromoxynil; however, each tank mix provided 97% to 100% control of S. arvensis. Results from this study demonstrate that co-application of ROS generators with mesotrione or tolpyralate controlled all broadleaf weed species >90% at 8 WAA, with the exceptions of A. artemisiifolia and C. album control with tolpyralate + glufosinate. Mesotrione plus PSII inhibitors controlled E. crus-galli and Setaria spp. 48 to 68 percentage points less than tolpyralate plus the respective PSII inhibitor at 8 WAA; however, mesotrione + glufosinate and tolpyralate + glufosinate controlled the grass weed species similarly.

Tolpyralate is commonly mixed with atrazine for improved control of common annual weed species in corn production systems in the United States and Canada. Weed control efficacy with this mixture is enhanced with the addition of methylated seed oil (MSO) Concentrate®; however, there is little information on the efficacy of tolpyralate + atrazine with other proprietary adjuvants. Therefore, four trials were conducted at field research sites in Ontario, Canada, to evaluate the efficacy of tolpyralate + atrazine when applied with six different commercially available adjuvants on four annual broadleaf and two annual grass weed species in corn. The adjuvants evaluated were MSO Concentrate®, Agral® 90, Assist® Oil Concentrate, Carrier®, LI 700®, and Merge®. A treatment of tolpyralate + atrazine applied with no adjuvant was also included in the study. For the control of velvetleaf and wild mustard, the adjuvants evaluated with tolpyralate + atrazine did not improve control. At 8 wk after application (WAA), the use of Agral® 90, Assist® Oil Concentrate, Carrier®, MSO Concentrate®, or Merge® with tolpyralate + atrazine provided similar or greater control of common ragweed than tolpyralate + atrazine applied with LI 700®. At 8 WAA, the adjuvants performed similarly with tolpyralate + atrazine for the control of common lambsquarters; however, LI 700® was the only adjuvant that did not improve control compared to tolpyralate + atrazine applied without an adjuvant. At 8 WAA, MSO Concentrate®, Carrier®, and Merge® improved control of barnyardgrass and foxtail species with tolpyralate + atrazine to a similar or greater level than Assist® Oil Concentrate, Agral® 90, and LI 700®. Overall, MSO Concentrate®, Carrier®, or Merge® should be added to tolpyralate + atrazine for control of the myriad of weed species interfering with corn production.

Tolpyralate is a 4-hydroxyphenylpyruvate dioxygenase–inhibiting herbicide that is applied postemergence for control of annual broadleaf and grass weeds in corn. Current Canadian label recommendations for tolpyralate specify the addition of a methylated seed oil (MSO) adjuvant (MSO Concentrate®) for improved weed control. The efficacy of tolpyralate applied with other proprietary adjuvants has not been widely reported in the peer-reviewed literature. Therefore, four field trials were conducted in corn over 2020 and 2021 in Ontario, Canada, to evaluate MSO Concentrate®, Agral® 90 (nonionic surfactant), Assist® Oil Concentrate (blended surfactant), Carrier® (blended surfactant), LI 700® (nonionic surfactant), and Merge® (blended surfactant) as adjuvants with tolpyralate for the control of annual broadleaf and grass weeds. At 8 wk after application (WAA), tolpyralate applied with MSO Concentrate®, Agral® 90, Assist® Oil Concentrate, Carrier®, or Merge® controlled velvetleaf, wild mustard, barnyardgrass, and foxtail species similarly. These adjuvants also enhanced the efficacy of tolpyralate similarly for the control of common ragweed at 8 WAA with the exception that Agral® 90 was inferior to Merge®. At 8 WAA, tolpyralate controlled common lambsquarters the greatest when applied with MSO Concentrate®, Agral® 90, Carrier®, or Merge®; these adjuvants with the exception of Agral® 90 were superior to Assist® Oil Concentrate. At 8 WAA, tolpyralate applied with LI 700® controlled common ragweed, barnyardgrass, and foxtail species less than when tolpyralate was applied with the other adjuvants tested; control of these weed species with tolpyralate was not improved with LI 700® when compared to tolpyralate applied without an adjuvant. Overall, tolpyralate applied with either MSO Concentrate®, Carrier®, or Merge® controlled all annual broadleaf and grass weed species similarly or greater than tolpyralate applied without an adjuvant or tolpyralate with Agral® 90, Assist® Oil Concentrate, or LI 700® at 8 WAA.

Glyphosate-resistant (GR) horseweed [Conyza canadensis (L.) Cronquist; syn.: Erigeron canadensis L.] interference can substantially reduce corn (Zea mays L.) yield. The complementary activity of 4-hydroxyphenylpyruvate dioxygenase (HPPD) and photosystem II (PSII) inhibitors has been investigated for the control of several weed species, and in many cases has been synergistic; however, there is little information on the interaction of HPPD- and PSII-inhibiting herbicides for postemergence control of GR C. canadensis in corn. Four field trials were studied over 2 yr (2019, 2020) in Ontario, Canada, in commercial corn fields with natural infestations of GR C. canadensis to evaluate the level of GR C. canadensis control with three HPPD-inhibiting herbicides (mesotrione, tolpyralate, and topramezone) and three PSII-inhibiting herbicides (atrazine, bromoxynil, and bentazon) applied individually and in tank-mix combinations, and to document the interaction of the three HPPD inhibitors tank mixed with the three PSII inhibitors. Mesotrione, tolpyralate, and topramezone controlled GR C. canadensis 83%, 84%, and 72%, respectively, at 8 wk after application (WAA). Bromoxynil and bentazon controlled GR C. canadensis 71% and 79%, respectively, while atrazine provided only 31% control at 8 WAA. The joint application of atrazine, bromoxynil, or bentazon with mesotrione increased GR C. canadensis control from 83% to 100% at 8 WAA. Tolpyralate tank mixed with atrazine, bromoxynil, or bentazon controlled GR C. canadensis 96%, 98%, and 98%, respectively, which was comparable to the mesotrione tank mixes at 8 WAA. Topramezone plus atrazine, bromoxynil, or bentazon controlled GR C. canadensis 91%, 93%, and 95%, respectively, at 8 WAA. Interactions between HPPD and PSII inhibitors were synergistic for all combinations of mesotrione or tolpyralate with atrazine, bromoxynil, or bentazon. The interaction between topramezone and PSII inhibitors was additive. All nine tank mixes controlled GR C. canadensis >90%. This study concludes that bromoxynil or bentazon, instead of atrazine, can be co-applied with mesotrione, tolpyralate, or topramezone without compromising GR C. canadensis control in corn.

Owing to the lack of effective POST herbicide options, producers typically rely on nicosulfuron as the main POST grass herbicide in sweet corn production systems. In 2019, a Wisconsin sweet corn producer reported fall panicum control escapes after spraying nicosulfuron. Seeds from mature plants were collected to (1) measure fall panicum response to acetolactate synthase (ALS)-inhibiting herbicides, (2) elucidate the resistance mechanism, and (3) evaluate its response to alternative POST herbicides. Greenhouse and laboratory investigations were conducted to assess fall panicum response to ALS-inhibiting herbicides and elucidate the resistance mechanism. Dose–response results showed that fall panicum was highly resistant to nicosulfuron with a resistance ratio of >12.9-fold (survived rates >254 g ai ha−1, or 8× the field label rate). Molecular and genetic studies indicated that there are multiple ALS gene copies in fall panicum and that resistance was due to a mutation in one copy, resulting in an Asp-376-Glu amino acid substitution. Additional greenhouse experiments indicate that clethodim (105 g ai ha−1), quizalofop-p-ethyl (70 g ae ha−1), glyphosate (864 g ae ha−1), and glufosinate (650 g ai ha−1) are effective POST options to manage the ALS-resistant fall panicum (>90.0% control and 96.8% biomass reduction) in rotational years. The 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides isoxaflutole (105 g ai ha−1), mesotrione (105 g ai ha−1), tembotrione (92 g ai ha−1), and tolpyralate (39 g ai ha−1) did not provide effective POST fall panicum control. Because these herbicides are commonly used for POST weed control in sweet corn, more investigations are required to evaluate combinations of HPPD-inhibiting herbicides with herbicides from other sites of action for POST fall panicum control. Herein we confirm the first case of herbicide resistance in fall panicum in the United States.

Four field experiments were completed in commercial corn fields during 2019 and 2020 to determine glyphosate-resistant (GR) horseweed control in corn with tiafenacil alone or in combination with bromoxynil, dicamba, or tolpyralate applied preplant (PP). Corn planted 1 to 10 d after herbicide application was not injured with any of the herbicides tested. GR horseweed interference reduced corn grain yield 32% when left uncontrolled. Herbicides reduced GR horseweed interference and resulted in corn grain yield that was similar to the weed-free control. Glyphosate (900 g ae ha−1) + tiafenacil at 12.5, 25, and 37.5 g ha−1 controlled GR horseweed 63%, 68%, and 72% at 4 wk after treatment (WAT) and decreased GR horseweed density 64%, 43%, and 83% and dry biomass 69%, 55%, and 83%, respectively. Glyphosate + tiafenacil at 12.5, 25, and 37.5 g ha−1 plus bromoxynil (280 g ai ha−1) controlled GR horseweed 81%, 88%, and 87% at 4 WAT and reduced GR horseweed density 82%, 94%, and 93% and dry biomass 93%, 93%, and 98%, respectively. Glyphosate + tiafenacil at 12.5, 25, and 37.5 g ha−1 plus dicamba (300 g ai ha−1) controlled GR horseweed 86%, 88%, and 88% at 4 WAT and decreased GR horseweed density 76%, 89%, and 86% and dry biomass 94%, 98%, and 98%, respectively. Glyphosate + tiafenacil at 12.5, 25, and 37.5 g ha−1 plus tolpyralate (30 g ai ha−1) controlled GR horseweed 90%, 90%, and 91% at 4 WAT and decreased GR horseweed density 93%, 91%, and 95% and dry biomass 98%, 97%, and 97%, respectively. The industry standards in Ontario, glyphosate + dicamba/atrazine and glyphosate + saflufenacil/dimethenamid-p controlled GR horseweed 95% and 100% at 4, 8, and 12 WAT and caused 99% and 100% density or biomass reduction, respectively.

Control of waterhemp is becoming more difficult in Ontario because biotypes have evolved resistance to four herbicide sites of action (SOA), including groups 2, 5, 9, and 14. The objective of this study was to compare PRE, POST, and PRE followed by (fb) POST herbicide programs for their effect on control, density, and biomass of multiple-herbicide–resistant (MHR) waterhemp as well as corn injury and grain yield. Two separate field studies, each consisting of five field trials, were conducted over a 2-yr period (2018 and 2019) in fields where corn was grown in Ontario, Canada. The first experiment evaluated MHR waterhemp control with an inhibitor of 4-hydroxyphenyl-pyruvate dioxygenase (HPPD) applied PRE, PRE fb glufosinate applied POST, and glufosinate applied POST. The second experiment evaluated MHR waterhemp control with a non-HPPD inhibitor applied PRE, then PRE fb a POST application of atrazine + mesotrione, and then atrazine + mesotrione applied POST. Atrazine + isoxaflutole caused 3% to 5% corn injury at environment 1 (E1); no corn injury was observed with PRE and POST herbicide programs at environments E2, E3, E4, and E5. In general, atrazine/bicyclopyrone/mesotrione/S-metolachlor and dimethenamid-P/saflufenacil applied PRE controlled MHR waterhemp ≥95% 12 wk after POST application (WAA). A POST application of glufosinate following atrazine + tolpyralate PRE, and a POST application of atrazine + mesotrione following atrazine/dicamba or atrazine/S-metolachlor PRE, improved control at 4, 8, and 12 WAA in most environments. In general, PRE fb POST applications resulted in better control of MHR waterhemp throughout the growing season than single PRE and POST applications (P < 0.05). We conclude that herbicide programs based on multiple effective SOAs may offer effective control of MHR waterhemp where field corn is grown. It is advisable that when choosing an herbicide application program that excellent control of MHR waterhemp should be the goal given its high fecundity and competitive ability.

This study consisting of six field experiments was conducted in 2018 and 2019 to evaluate the tolerance of four corn hybrids (P9998AM, P9840AM, DKC42-60RIB, and DKC43-47RIB) to the tank mix of tolpyralate + atrazine with a commercial glyphosate formulation. At 1 wk after application (WAA), two corn hybrids (P9998AM and P9840AM) exhibited more injury from tolpyralate + atrazine (2× rate) applied alone and in combination with glyphosate than DKC42-60RIB and DKC43-47RIB; however, all corn hybrids responded similarly with respect to visible injury 2, 4, and 8 WAA, stand loss, and yield. Application of tolpyralate + atrazine or glyphosate + tolpyralate + atrazine at the 2× rate caused greater corn injury (up to 27%) than tolpyralate + atrazine or glyphosate + tolpyralate + atrazine at the 1× rate (up to 8%) at 1 WAA. The addition of commercial glyphosate with tolpyralate + atrazine did not enhance injury symptoms. Results of this study show that there is a wide margin of corn safety with tolpyralate + atrazine applied alone and in combination with a commercial formulation of glyphosate.

Tolpyralate is a new 4-hydroxyphenyl-pyruvate dioxygenase (HPPD)–inhibiting herbicide for weed control in corn. Previous research has reported efficacy of tolpyralate + atrazine on several annual grass and broadleaf weed species; however, no studies have evaluated weed control of tolpyralate + atrazine depending on time-of-day (TOD) of application. Six field experiments were conducted over a 2-yr period (2018, 2019) near Ridgetown, ON, to determine if there is an effect of TOD of application on tolpyralate + atrazine efficacy on common annual grass and broadleaf weeds. An application was made at 3-h intervals beginning at 06:00 h with the last application at 24:00 h. There was a slight TOD effect on velvetleaf, pigweed species, and common ragweed control with tolpyralate + atrazine; however, the magnitude of change throughout the day was ≤3% at 2, 4, or 8 wk after application (WAA). There was no effect of TOD of tolpyralate + atrazine on the control of lambsquarters, barnyardgrass, and green foxtail. All weed species were controlled ≥88% at 8 WAA. There was no effect of TOD of tolpyralate + atrazine application on corn yield. Results of this study show no evidence of a TOD effect on weed control efficacy with tolpyralate + atrazine.

Effective POST herbicides and herbicide mixtures are key components of integrated weed management in corn; however, herbicides vary in their efficacy based on application timing. Six field experiments were conducted over 2 yr (2017–2018) in southwestern Ontario, Canada, to determine the effects of herbicide application timing and rate on the efficacy of tolpyralate, a new 4-hydroxyphenyl pyruvate dioxygenase inhibitor. Tolpyralate at 15, 30, or 40 g ai ha−1 in combination with atrazine at 500 or 1,000 g ai ha−1 was applied PRE, early POST, mid-POST, or late POST. Tolpyralate + atrazine at rates ≥30 + 1,000 g ha−1 provided equivalent control of common lambsquarters and Powell amaranth applied PRE or POST, whereas no rate applied PRE controlled common ragweed, velvetleaf, barnyardgrass, or green foxtail. Common ragweed, common lambsquarters, velvetleaf, and Powell amaranth were controlled equally regardless of POST timing. In contrast, control of barnyardgrass and green foxtail declined when herbicide application was delayed to the late-POST timing, irrespective of herbicide rate. Similarly, corn grain yield declined within each tolpyralate + atrazine rate when herbicide applications were delayed to late-POST timing. Overall, the results of this study indicate that several monocot and dicot weed species can be controlled with tolpyralate + atrazine with an early to mid-POST herbicide application timing, before weeds reach 30 cm in height, and Powell amaranth and common lambsquarters can also be controlled PRE. Additionally, this study provides further evidence highlighting the importance of effective, early-season weed control in corn.

Horseweed biotypes resistant to glyphosate and ALS-inhibiting herbicides are becoming more prevalent in Canada and the United States and present a significant management challenge in field crops. Tolpyralate is a recently commercialized herbicide for use in corn that inhibits 4-hydroxyphenylpyruvate dioxygenase (HPPD), and there is little information regarding its efficacy on horseweed. Six field experiments were conducted in 2017 and 2018 at four locations in Ontario, Canada, to determine the biologically effective dose of tolpyralate and tolpyralate + atrazine and to compare label rates of tolpyralate and tolpyralate + atrazine to currently accepted herbicide standards for POST control of glyphosate and cloransulam-methyl resistant (MR) horseweed. At 8 wk after application (WAA), tolpyralate at 4.8 and 22.6 g ha–1 provided 50% and 80% control, respectively. When applied with atrazine at a 1:33.3 tank-mix ratio, 22.3 + 741.7 g ha–1 provided 95% control of MR horseweed. The addition of atrazine to tolpyralate at label rates improved control of MR horseweed to 98%, which was similar to the control provided by dicamba:atrazine and bromoxynil + atrazine. The results of this study indicate that tolpyralate + atrazine provides excellent control of MR horseweed POST in corn.

Tolpyralate is a new Group 27 pyrazolone herbicide that inhibits the 4-hydroxyphenyl-pyruvate dioxygenase enzyme. In a study of the biologically effective dose of tolpyralate from 2015 to 2017 in Ontario, Canada, tolpyralate exhibited efficacy on a broader range of species when co-applied with atrazine; however, there is limited published information on the efficacy of tolpyralate and tolpyralate+atrazine relative to mesotrione and topramezone, applied POST with atrazine at label rates, for control of annual grass and broadleaf weeds. In this study, tolpyralate applied alone at 30 g ai ha−1 provided >90% control of common lambsquarters, velvetleaf, common ragweed, Powell amaranth/redroot pigweed, and green foxtail at 8 weeks after application (WAA). Addition of atrazine was required to achieve >90% control of wild mustard, ladysthumb, and barnyardgrass at 8 WAA. Tolpyralate+atrazine (30+1,000 g ai ha−1) and topramezone+atrazine (12.5+500 g ai ha−1) provided similar control at 8 WAA of the eight weed species in this study; however, tolpyralate+atrazine provided >90% control of green foxtail by 1 WAA. Tolpyralate+atrazine provided 18, 68, and 67 percentage points better control of common ragweed, green foxtail, and barnyardgrass, respectively, than mesotrione+atrazine (100+280 g ai ha−1) at 8 WAA. Overall, tolpyralate+atrazine applied POST provided equivalent or improved control of annual grass and broadleaf weeds compared with mesotrione+atrazine and topramezone+atrazine.

Tolpyralate is a new 4-hydroxyphenyl-pyruvate dioxygenase (HPPD)-inhibiting herbicide for POST weed management in corn; however, there is limited information regarding its efficacy. Six field studies were conducted in Ontario, Canada, over 3 yr (2015 to 2017) to determine the biologically effective dose of tolpyralate for the control of eight annual weed species. Tolpyralate was applied POST at six doses from 3.75 to 120 g ai ha−1 and tank mixed at a 1:33.3 ratio with atrazine at six doses from 125 to 4,000 g ha−1. Regression analysis was performed to determine the effective dose (ED) of tolpyralate, and tolpyralate+atrazine, required to achieve 50%, 80%, or 90% control of eight weed species at 1, 2, 4, and 8 wk after application (WAA). The ED of tolpyralate for 90% control (ED90) of velvetleaf, common lambsquarters, common ragweed, redroot pigweed or Powell amaranth, and green foxtail at 8 WAA was ≤15.5 g ha−1; however, tolpyralate alone did not provide 90% control of wild mustard, barnyardgrass, or ladysthumb at 8 WAA at any dose evaluated in this study. In contrast, the ED90 for all species in this study with tolpyralate+atrazine was ≤13.1+436 g ha−1, indicating that tolpyralate+atrazine can be highly efficacious at low field doses.

Control of multiple-resistant Palmer amaranth populations in corn will rely heavily on the use of POST 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides. Therefore, field and greenhouse experiments were conducted to: (1) evaluate Palmer amaranth control with four HPPD inhibitors alone and in combination with atrazine at two application timings and (2) investigate the joint activity of HPPD-inhibiting herbicides and atrazine in atrazine-resistant (AR) and atrazine-susceptible (AS) Palmer amaranth populations. Control of the AR Palmer amaranth population varied among the HPPD-inhibiting herbicides with tolpyralate>tembotrione=topramezone>mesotrione based on GR50 values in the greenhouse. In the field, Palmer amaranth control was lower when the HPPD-inhibiting herbicides, with the exception of tolpyralate, were applied to 15- vs. 8-cm-tall Palmer amaranth. Tolpyralate controlled Palmer amaranth ≥95% at both application timings. The addition of atrazine (560 g ai ha−1) improved Palmer amaranth control with mesotrione and topramezone at the 8-cm application timing and with mesotrione and tembotrione at the 15-cm application timing. In the greenhouse, joint activity of mesotrione and atrazine and tembotrione and atrazine was synergistic with both the AR and AS Palmer amaranth populations. In the AR population, an additional 980 g ai ha−1 of atrazine (8X) was needed to cause a synergistic response compared with the AS population. Synergistic responses with mesotrione were detected with all atrazine rates for the AS population and for atrazine rates ranging from 280 to 2,240 g ai ha−1 for the AR population. Only additive responses were observed when atrazine was applied with tolpyralate and topramezone, indicating that joint activity in the form of synergism occurs more readily with the triketones compared with the benzopyrazoles. When faced with an AR Palmer amaranth population, the addition of atrazine to HPPD inhibitors may increase the overall success of weed management due to joint activity.