Tidal embayments in the Bay of Biscay (France) host nursery grounds where common sole, Solea solea, is the most abundant flatfish species. This study aimed to appraise the way those habitats function as nurseries through juvenile sole's responses in somatic growth and condition (Fulton's K) during their first year of occupancy. Field data, two yearly trawling series, taken monthly, were compared with a 6-month-long mesocosm experiment involving reared fish of the same wild origin. Growth rates were compared with predicted maximum growth according to an experimentally established model in relation to temperature. In the field, 0-group sole total length (TL) averaged 130 mm from September onwards in 1999 and 2000. Mean growth rates were 0.7–0.9 mm day−1 in summer and ≤ 0.1 mm day−1 in autumn, when the model predicted 1.4 and 0.9 mm day−1, respectively. In the mesocosm, the growth rates were 0.9 and 0.4 mm day−1 for the same seasons and for stocking densities (300 fish 1000 m−2) about 10 times higher than usually recorded in the field. Wild sole remained in medium condition (K ca. 1 g cm−3) during the entire growing season, whereas they attained a higher K after being released into the mesocosm (1.2–1.3 g cm−3). During the mesocosm experiment, growth was shown to be primarily temperature-controlled: fish reached 150–160 mm (TL) in November. From this investigation, it can be concluded that sole's responses in condition and growth are altered over the whole growing season on nursery grounds. The situation is exacerbated in autumn when the sum of abiotic constraints increases as the competitive biomass does. This indicates impairment of the nursery functioning which, in the context of fish habitat conservation, emphasises the particular vulnerability of embayments to the addition of any further constraint.

Bioassays were carried out with the ‘blue diatom' Haslea ostrearia Simonsen, which is responsible for oyster greening during the fattening period of Crassostrea gigas Thunberg in oyster ponds. Samples of seawater were taken from two oyster ponds: one without oysters and the other with 20 oysters per m[sup2 ], maximal density allowed by the French AFNOR norm for ‘refinement'. The aims were to clarify the nutrient requirements of this diatom, also to elucidate the eventual influence of C. gigas at this density on the seawater fertility and to envisage the mass production of this diatom by pond fertilization. Examination of cell numeric densities at the end of bioassays allows us to conclude that silicate was the first limiting nutrient, closely followed by phosphate. Chlorophyll a concentrations led to different conclusions: phosphate was the first limiting factor, but after the seawater storage period in ponds, seawater quality evolved to a deficiency of nitrogen. Silicate addition increased cell division rate, and silicate depletion increased chl a synthesis for this species. Examination of nutrient assimilation ratios confirms that H. ostrearia requires a large amount of silicon. From these results, it was possible to prepare a N + P + Si simplified medium which has been tested in laboratory and field mesocosm conditions. In both conditions, similar results were observed: a significant increase in H. ostrearia cell concentrations and consequently an evolution up to the greening stage. Applications of this work are numerous; the principal permits us to envisage the production of this species in 25-m3 ponds, with the aim of allowing constant production of the greening phenomenon.