Milling behaviour is problematic when using hydroacoustics to estimate the number of migrating fish in rivers. Milling behaviour was observed for adult sockeye salmon migrating upstream in the Wannock River, to their spawning grounds in the tributaries of Owikeno Lake in the central coast area of British Columbia, Canada. We classified the acoustic salmon tracks to separate the milling fish from the actively migrating fish in an attempt to obtain an estimate of sockeye salmon flux as they migrate to their spawning grounds. We used discriminant function analysis and found that three variables measured on each track were sufficient for the classification of the acoustic tracks into milling and non-milling categories with an approximate classification accuracy of 98%. The method we present can also be used to separate tracks of targets of interest from noise or debris tracks that occur in the acoustic data, if discrete track characteristics are ascertained.

Tidal embayments in the Bay of Biscay (France) host nursery grounds where common sole, Solea solea, is the most abundant flatfish species. This study aimed to appraise the way those habitats function as nurseries through juvenile sole's responses in somatic growth and condition (Fulton's K) during their first year of occupancy. Field data, two yearly trawling series, taken monthly, were compared with a 6-month-long mesocosm experiment involving reared fish of the same wild origin. Growth rates were compared with predicted maximum growth according to an experimentally established model in relation to temperature. In the field, 0-group sole total length (TL) averaged 130 mm from September onwards in 1999 and 2000. Mean growth rates were 0.7–0.9 mm day−1 in summer and ≤ 0.1 mm day−1 in autumn, when the model predicted 1.4 and 0.9 mm day−1, respectively. In the mesocosm, the growth rates were 0.9 and 0.4 mm day−1 for the same seasons and for stocking densities (300 fish 1000 m−2) about 10 times higher than usually recorded in the field. Wild sole remained in medium condition (K ca. 1 g cm−3) during the entire growing season, whereas they attained a higher K after being released into the mesocosm (1.2–1.3 g cm−3). During the mesocosm experiment, growth was shown to be primarily temperature-controlled: fish reached 150–160 mm (TL) in November. From this investigation, it can be concluded that sole's responses in condition and growth are altered over the whole growing season on nursery grounds. The situation is exacerbated in autumn when the sum of abiotic constraints increases as the competitive biomass does. This indicates impairment of the nursery functioning which, in the context of fish habitat conservation, emphasises the particular vulnerability of embayments to the addition of any further constraint.

GAMEFISTO simulation model is presented as a tool to improve the small scale fisheries bioeconomic simulation techniques. The main novelty of the current model is the implementation of game theoretic techniques for forecasting the fishing effort trends and consequently, the fish population levels and the economic outcome, including landings, income and net profits. The model assigns individual fishing strategies to individual vessels according to their technical characteristics. The fishermen within a fishing fleet exploiting a single stock are assumed to be the decision agents, who share not only a fish population (modelled through stock externality) but also a market, through an offer-demand function (market externality). Mediterranean fisheries need to be analysed at vessel level due to the heterogeneity of its fishing fleets. A ten year simulation on red shrimp, Aristeus antennatus, is presented as an application of the presented GAMEFISTO model.

Decommissioning programmes have been used in many countries in an attempt to reduce the level of overexploitation in fisheries. The extent to which human capital enhancement may offset capacity reductions, however, has not been previously examined. The study uses a stochastic production frontier model to estimate the impact of differing skipper, vessel and technology characteristics on the productivity of a set of UK trawlers operating in the English Channel. The results suggest that productivity improvements resulting from increased education and training could exceed those from increased technological adoption. Increased investment in human capital enhancement could potentially offset, at least to some degree, the effects of decommissioning in the fishery. The study highlights an apparent oversight in fisheries policy analysis. Considerable attention is paid to the potential problem of technological creep and input substitution. However, enhancing human capital may have a greater impact on stocks than technological adoption in established fisheries.

The development and application of genomics has been facilitated in a number of fields by the availability of new methodologies and tools, such as high throughput DNA sequencing and complementary DNA (cDNA) microarrays. Genomic tools are already used in research on commercially important fish and shellfish species. Thousands of expressed sequence tags (EST) are now available for some of these species, and the sequencing of complete genomes of tilapia, cod, salmonids, flatfishes, sea bass and Pacific oyster has been proposed. Microarray technology through simultaneous analysis of the expression of thousands of genes allows the identification of candidate genes involved in the function of multiple physiological, morphological and behavioural traits of interests in organisms and populations from different environments. This paper reviews the current development of genomic technologies, and pinpoints their potential beneficial applications as well as implications for fisheries management and aquaculture.

We tested cross-species amplification of 68 existing microsatellite loci in 6 species of the Sparidae family: Acanthopagrus butcheri, Sparus aurata, Pagrus auratus, Chrysophrys major, Pagellus bogaraveo, Pagellus erythrinus and one species of Bothidae, Paralichthys olivaceus. Of the 68 loci screened, sixteen were found to be polymorphic when tested in 20 individual black sea bream, Acanthopagrus schlegeli. The number of alleles per locus ranged from 2 to 9, and the observed and expected heterozygosity ranged from 0.55 to 0.95, and from 0.58 to 0.87, respectively. Our results show that cross-species amplification of known microsatellite loci in closely related species is a highly promising source of microsatellite markers for A. schlegeli.

The gilthead seabream is the most important Mediterranean aquacultured fish species. The main objective of this study was to investigate whether copper sulphate bath treatments used routinely in aquaculture have effects on important physiological functions of early life stages of the gilthead seabream (Sparus aurata). Fingerlings (80–90 days, 0.27 ± 0.06 g) were exposed to copper sulphate baths at 0, 0.25, 0.5 and 1.5 mg L−1 during 24 h. Effects on the central nervous function were evaluated analysing brain acetylcholinesterase activity (AChE). Oxidative stress was assessed by the quantification of lipid peroxidation (LP). Heat shock proteins (HSP70) were used as a general response to chemical stress, RNA/DNA ratio as an indicator of growth, and effects on detoxification function were estimated using glutathione–S-transferases activity (GST). Exposure of S. aurata fingerlings to copper sulphate induced increased lipid peroxidation. In contrast, there were no significant changes in AChE activity, HSP70 levels, RNA/DNA ratio or GST activity. Particularly, the absence of response in GST activity points towards a limited extent of the putative damage caused by the treatments at the highest doses (evidenced through the levels of peroxidation), since this enzyme is directly implicated in detoxification processes involving redox cyclic products. The results suggest that copper sulphate baths at the conditions tested are safe treatments for S. aurata fingerlings.

Seasonal changes in feeding habits and diet of the cockle Cerastoderma glaucum (Mollusca, Bivalvia) were analysed using carbon (δ 13C) and nitrogen (δ 15N) stable isotopes. I aimed to investigate the role of benthic and pelagic sources in the diet of this dominant infaunal bivalve on a western Mediterranean sandy bottomed pond. Adult C. glaucum and all potential organic sources (particulate and sedimentary organic matter, seagrass, macroalgae, heterotrophic detritus) were collected and analysed for δ 13C and δ 15N. In total 5 dominant organic sources were found, ranging between –21.0 and –8.0 ‰ for δ 13C and from 3.0 to about 7.0% for δ 15N. C. glaucum assimilated fraction ranged between –14.0/–15.0% and 6.0/9.0% respectively, for carbon and nitrogen, while its estimated fractionation seasonally varied between 1 and 1.5 for carbon and was about 3 for nitrogen. C. glaucum reflected the isotopic composition of its food while maintaining, throughout the year, a fairly constant isotopic composition in its assimilated fraction, despite the high variability in isotopic composition of available sources.

This study was designed to assess in situ the effects of sediment nearness on Crassostrea gigas metabolism. One year-old oysters were reared for 5 months (April-August) in plastic bags on metallic frames at 10 and 60 cm off the sediment at the beginning of April. The management of the energetic resources (storage, consumption) was estimated with respect to biochemical changes of proteins, lipids and carbohydrates. In order to evaluate the effect of the sediment nearness at cellular level, the metabolic rate was evaluated by assessing pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK) enzyme activities and electron transport system (ETS) activity. The metabolic pathways were assessed by measuring ATP and intermediary metabolites such as alanine, succinate and aspartate. Despite similar survival and growth at the two rearing levels, the protein, lipid and carbohydrate content were significantly lower in the oysters close to the bottom. In the oysters reared at 10 cm, PK activity was also significantly reduced and a significant negative correlation between alanine content and PK activity was revealed. The sediment nearness decreased significantly the ATP content in the oyster. There was a significant relationship between ATP and aspartate in these oysters. The use of aspartate contributed to complete the metabolic pathways along with carbohydrate allowing to maintain the same biological performance as the oyster reared far from the bottom. The energy state (proteins, lipids, carbohydrates and ATP contents) could suggest that the oysters close to the sediment were fed less but the metabolic enzyme activities allow to suggest an oxygen deficiency. However, the reduced PK activity, the absence of PEPCK activity stimulation and the utilization of aspartate suggest a transition stage to anaerobiosis.