Fall applications of fenarimol on hybrid Bermudagrass (Cynodon dactylon × C. transvaalensis) provide effective preemergence Poa annua (L.) control and suppress Ophiosphaerella spp. pathogens; however, concerns exist for turf injury and root growth restrictions. Two 60-d greenhouse studies were conducted to evaluate the effects of fenarimol at 0, 1.6, and 3.2 kg ai/ha per 30 d with and without trinexapac-ethyl (TE) at 0.017 kg ai ha/15 d on ‘TifEagle’ Bermudagrass. Turf color was enhanced by TE 14 d after initial treatment (DAIT) and was continually superior throughout the experiment. Fenarimol at 3.2 kg/ha per 30 d decreased turf color 14 DAIT, but was similar to nontreated turf on all other observation dates. Increased fenarimol rates applied twice caused approximately 10% injury at 42, 49, and 56 d after treatment; however, injury was acceptable after initial and repeat applications. TE reduced clipping yield an average 39% from six sampling dates. Initial fenarimol applications (without TE) reduced clippings by 37% 20 DAIT and repeated applications reduced clippings 40, 50, and 60 DAIT. Increased fenarimol rate linearly decreased root mass for turf treated with and without TE; however, Bermudagrass receiving TE averaged 23% enhanced root mass 60 DAIT over all fenarimol rates. Bermudagrass receiving fenarimol at 0, 1.6, and 3.2 kg/ ha per 30 d with TE averaged 27, 24, and 16% higher root mass, respectively, compared to turf receiving fenarimol without TE. Treatments had no influence on root length. Results indicate that two consecutive fenarimol applications at 1.6 and 3.2 kg/ha per 30 d may cause minor injury to TifEagle Bermudagrass and restrict root growth. Repeated TE applications, however, could decrease injury from fenarimol and enhance rooting relative to fenarimol applied exclusively.

Greenhouse experiments were conducted to determine whether multiple applications of glyphosate and time of glyphosate application with regard to the crop's growth stage had a significant effect on the growth and development of glyphosate-resistant canola. Glyphosate was applied as single applications at the two-, four-, or six-leaf stage of canola; as sequential double applications at the two- and four-, two- and six-, and four- and six-leaf stages of canola; and as a triple application at all three stages. Of the plant growth parameters measured, single applications of glyphosate resulted in significant reductions to stem weight and shoot weight compared with nontreated plants, and multiple applications of glyphosate caused significant reductions to leaf area, leaf weight, stem weight, and shoot weight. Single applications of glyphosate were less injurious to glyphosate-resistant canola compared with multiple applications, and canola growth parameter reductions were greatest after earlier glyphosate applications.

Composition and abundance of weed populations often change in response to new or extensively used weed management practices. Glyphosate-resistant (GR) technology is one such weed management practice now used extensively. A recent survey of weed scientists was conducted to address weed shifts in GR corn, cotton, and soybean. Twelve scientists in 11 states responded to the survey. Averaged over estimates from scientists, GR corn, cotton, and soybean were planted on 15, 90, and 88% of the hectarage in 2003, respectively. Acreage of GR corn is expected to rise, whereas only minor changes in acreage of GR cotton or soybean are expected. Weed shifts have not been observed in GR corn but have occurred in GR cotton and soybean. In GR cotton, Amaranthus, Commelina, Ipomoea, and Cyperus species as well as annual grasses were noted as becoming more problematic. Similar to cotton, Ipomoea and Commelina species are becoming more troublesome in GR soybean. In addition, in GR soybean, various winter annuals, lambsquarters species, and waterhemp species were noted as becoming more problematic. All scientists felt that weeds shifts were occurring, and two-thirds of these scientists noted that weed shifts are currently of economic concern. The scientists recommend the following to help manage weed shifts: additional herbicides in mixture with glyphosate, rotation to herbicides other than glyphosate, rotation to non–GR crops, and greater use of soil-applied herbicides.

Field studies were conducted to evaluate weed control in herbicide-resistant canola in Georgia. The resistant canola cultivars and respective herbicides were ‘Pioneer 45A76’ and imazamox, ‘Hyola 357RR’ and glyphosate, and ‘2573 Invigor’ and glufosinate. Weed seed of Italian ryegrass and wild radish were sown simultaneously in October with canola and control of these species was evaluated along with other naturally occurring weeds. Herbicide treatments for the respective herbicide-resistant canola cultivar were imazamox at 0.035 and 0.071 kg ai/ha, glyphosate at 0.84 and 1.64 kg ae/ha, and glufosinate at 0.5 and 1.0 kg ai/ha. Herbicides were applied at one– two-leaf (LF) and three–four-LF canola stages. There was no significant injury to any canola cultivar as a result of herbicide rate or timing of application. By midseason (February), imazamox effectively controlled wild radish, henbit, and shepherd's-purse at both rates and at both timings. When applied to three–four-LF canola, the higher rates of glyphosate and glufosinate were required to provide 75% or greater control of Italian ryegrass, wild garlic, and henbit. Glufosinate did not adequately control wild radish at either rate or application timing. Greenhouse experiments provided similar results.

A field experiment was conducted near Davis, CA, during the 2003 and 2004 summer growing seasons to compare weed control, yield, and fruit quality in different irrigation and tillage systems in processing tomato. Trial design was a subplots with the main plots as subsurface drip irrigation or furrow irrigation, subplots were standard tillage or conservation tillage, and sub-subplots were herbicide or no herbicide. The hypothesis was that subsurface drip irrigation could limit surface soil wetting and thus inhibit germination and growth of weeds equal to or better than standard tillage and/or herbicides. In both 2003 and 2004, weed densities in the subsurface drip irrigation treatments were over 98% lower than the levels in furrow irrigation treatments. In addition, weed densities were lower in the subsurface drip–conservation till–no herbicide treatment than in any of the furrow irrigation treatments, including the furrow irrigation–standard tillage–herbicide treatments. The time required for a hand-hoeing crew to remove weeds was 5 to 13 times greater in furrow irrigation treatments compared to subsurface drip irrigation treatments. Weed biomass on beds at tomato harvest was 10 to 14 times greater in the furrow systems as compared to the subsurface drip irrigation systems. These results demonstrate the effectiveness of subsurface drip irrigation in controlling weed germination and growth, compared to tillage or herbicide applications. Tomato yield was higher in the subsurface drip irrigation treatment compared to furrow irrigation in 2004. Herbicide treatment increased yield in 2004, but only in the furrow irrigation treatment in 2003. Fruit brix level was not related to treatment in 2003, but was lower in the subsurface drip irrigation plots in 2004. These results indicate that subsurface drip irrigation can reduce weed competition in conservation tillage systems, without requiring herbicide applications.

Three field trials were established from 2001 to 2003 in Ontario to determine the effect of foramsulfuron POST (35 and 70 g ai/ha), isoxaflutole PRE (105 and 210 g ai/ha), and isoxaflutole plus atrazine PRE (105 + 1063 and 210 + 2126 g ai/ha) applied in the previous years to field corn on cranberry, black, kidney, and white (navy) bean. Foramsulfuron residues did not cause visible injury, or reductions in shoot dry weight or yield of dry bean 1 yr after application in corn. In contrast, visual injury across the four market classes varied from 4 to 37% 1 yr after application of isoxaflutole, and from 30 to 54% 1 yr after application of isoxaflutole plus atrazine. Isoxaflutole residues reduced shoot dry weight and yield as much as 81 and 44% in cranberry, 52 and 39% in black, 53 and 19% in kidney, and 42 and 19% in white bean, respectively. Isoxaflutole plus atrazine residues reduced shoot dry weight and yield as much as 87 and 64% in cranberry, 75 and 61% in black, 71 and 46% in kidney, and 65 and 33% in white navy bean, respectively. Injury was not detected regardless of market classes 2 yr after application of isoxaflutole alone or in tank mix with atrazine. Based on these results, it is recommended that none of the market classes of dry bean tested in this study should be grown 1 year after an application of isoxaflutole or isoxaflutole plus atrazine. A recropping interval of 2 years is currently recommended following applications of isoxaflutole or isoxaflutole plus atrazine for these market classes of dry bean.

Field studies were conducted at three locations to evaluate glyphosate-resistant (GR) cotton response, weed control, and cotton lint yields to two formulations of glyphosate (diammonium salt– glyphosate and isopropylamine salt–glyphosate) and trifloxysulfuron applied early postemergence (EPOST) alone or to tank mixtures of trifloxysulfuron with each glyphosate formulation, with and without a late postemergence-directed (LAYBY) treatment of prometryn plus MSMA. Trifloxysulfuron and both formulations of glyphosate controlled common lambsquarters and pitted morningglory. Both glyphosate formulations provided equivalent control of common lambsquarters, goosegrass, pitted morningglory, prickly sida, and smooth pigweed. Trifloxysulfuron controlled smooth pigweed better than either glyphosate formulation but did not control goosegrass or prickly sida. Prometryn plus MSMA LAYBY improved late-season control of common lambsquarters, goosegrass, large crabgrass, and pitted morningglory for all EPOST systems and improved late-season smooth pigweed control for EPOST systems that did not include trifloxysulfuron. Cotton injury was 2% or less from both glyphosate formulations, while trifloxysulfuron injured ‘Deltapine 5415RR’ 7 to 16% at two locations. At a third location, trifloxysulfuron injured ‘Paymaster 1218RR/BG’ 24%, and when applied in mixture with either glyphosate formulation, injury increased to at least 72%. Cotton injury was transient at the first two locations and was not visually apparent 3 to 5 wk later. Cotton yield at the third location was reduced. High cotton yields reflected high levels of weed control.

Resistance to the herbicide glyphosate is currently known in at least eight weed species from many countries. Some populations of goosegrass from Malaysia, rigid ryegrass from Australia, and Italian ryegrass from Chile exhibit target site–based resistance to glyphosate through changes at amino acid 106 of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene. Mutations change amino acid 106 from proline to either serine or threonine, conferring an EPSPS weakly resistant to glyphosate. The moderate level of resistance is sufficient for commercial failure of the herbicide to control these plants in the field. Conversely, a nontarget site resistance mechanism has been documented in glyphosate-resistant populations of horseweed and rigid ryegrass from the United States and Australia, respectively. In these resistant plants, there is reduced translocation of glyphosate to meristematic tissues. Both of these mechanisms are inherited as a single, nuclear gene trait. Although at present only two glyphosate-resistance mechanisms are known, it is likely that other mechanisms will become evident. The already very large and still increasing reliance on glyphosate in many parts of the world will inevitably result in more glyphosate-resistant weeds, placing the sustainability of this precious herbicide resource at risk.

In the state of Illinois, waterhemp and smooth pigweed are among the worst agricultural weeds. Previous research shows high potential for hybridization between these two species. However, the actual occurrence of hybrids in natural settings is still uncertain. Morphological similarity between hybrids and waterhemp makes field surveys of hybrids difficult to conduct. The main purpose of this study was to characterize the morphology of waterhemp × smooth pigweed F1 hybrids, emphasizing evaluation of characters that may allow for hybrid discrimination in field Amaranthus communities. Concurrently, the study characterized hybrid reproductive fitness, chromosome number, and DNA content. To accomplish this, hybrids were obtained from field crosses. A species-specific polymorphism in the ALS gene was used to verify hybrid identity. Significant differences (α = 0.05) between hybrids and individuals of the parental species were observed for five staminate and five carpellate characters. Of these, five characters differentiated hybrids from waterhemp. However, clustering analyses using these characters indicated that morphological differences were not reliable enough, by themselves, for unambiguous hybrid identification. Also, hybrid homoploidy (2n = 32) with respect to parental species excluded chromosome counts in hybridity determinations. However, DNA content analysis may be used for such purpose. Hybrids had an average of 1.21 pg of DNA per 2C nucleus, a value intermediate to that of parental species. Hybrids produced 3.3 or 0.7% the seed output of parental and sibling waterhemp individuals, respectively. Percent micropollen in hybrids was 95-times greater than in parental species. Hybrid sterility appears to be the most reliable feature for hybrid discrimination when conducting field surveys. However, molecular and cytogenetic analyses as employed in this study may be desired for ultimate identity corroboration.

Red rice is a major weed of rice in the southern U.S. and can intercross with rice. Knowledge of the plant phenotypes from such crosses would be valuable for identification and management of these plants. Male-sterile long-grain tropical japonicas ‘Kaybonnet-1789’ and ‘Cypress-1819’ were crossed with two awned and two awnless U.S. red rice types. F1 plants produced pubescent leaves, red pericarp, and medium-grain seeds. Crosses involving awned LA3 and TX4 red rice produced F1 plants with reddish-purple basal leaf sheaths and usually flowered within the same time periods as the parents, whereas those involving awnless StgS red rice had green basal leaf sheaths, flowered much later than either parent, and produced awnless F1 and F2 offspring. Crosses involving awned red rice produced F1 plants with long awns and F2 plants with awns ranging in length from zero to that of red rice parents. F1 plants were taller than either parent and produced intermediate culm angles similar to red rice, whereas F2 plants had culms ranging from erect (like rice) to more open than red rice. Thus, true F1 hybrids from crosses between pure breeding (homozygous) rice and red rice can be positively identified by a combination of traits including pubescent leaves, medium-grain seeds with red pericarps, open plant types, and heights greater than the red rice parent. F1 hybrids may be awned or awnless, have purple or green stems, or have normal or delayed heading. F2 plants have a broad combination of phenotypic traits found in both parents and F1 hybrids.

Field studies were conducted at three locations during both 2002 and 2003 to evaluate weed control and response of glyphosate-resistant (GR) corn to glyphosate or nicosulfuron plus atrazine applied POST at three application timings with and without alachlor plus atrazine applied PRE. The POST herbicides were applied timely (5- to 9-cm weeds) or applications were delayed 1 or 2 wk. All treatments, except the weedy check, were followed by glyphosate postemergence-directed (PDIR) 4 wk after the timely POST application. Common lambsquarters, common ragweed, Palmer amaranth, prickly sida, and smooth pigweed were controlled at least 94% regardless of PRE or POST treatments. Large crabgrass and fall panicum were controlled at least 96% by glyphosate regardless of PRE herbicide or POST application timing. In contrast, control by nicosulfuron plus atrazine POST in the absence of PRE herbicide decreased as application was delayed. Sicklepod was controlled at least 94% when POST herbicides were applied timely, but control by both POST herbicide treatments decreased with delayed application regardless of PRE herbicide. Tall morningglory was controlled 93% or greater by POST herbicides applied timely. Control by both POST herbicide treatments decreased as application was delayed, with glyphosate being affected more by timing than nicosulfuron plus atrazine. Corn grain yield was similar with glyphosate and nicosulfuron plus atrazine. Yield was unaffected by POST application timing when PRE herbicides were included. Without PRE herbicide, grain yield decreased as POST herbicide application was delayed.

Glyphosate, a nonselective herbicide and also the world's most widely used herbicide, inhibits 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), an enzyme in the aromatic amino acid biosynthetic pathway. Because of its broad-spectrum and potent weed control and favorable environmental characteristics, attempts to engineer glyphosate resistance have been intensive in the past few decades. The use of at least three different mechanisms has conferred glyphosate resistance in normally sensitive crop species. Early work focused on progressive adaptation of cultured plant cells to stepwise increases in glyphosate concentrations. The resulting cells were resistant to glyphosate because of EPSPS overexpression, EPSPS gene amplification, or increased enzyme stability. Further work aimed to achieve resistance by transforming plants with glyphosate metabolism genes. An enzyme from a soil microorganism, glyphosate oxidoreductase (GOX), cleaves the nitrogen– carbon bond in glyphosate yielding aminomethylphosphonic acid. Another metabolism gene, glyphosate N-acetyl transferase (gat), acetylates and deactivates glyphosate. A third mechanism, and the one found in all currently commercial glyphosate-resistant crops, is the insertion of a glyphosate-resistant form of the EPSPS enzyme. Several researchers have used site-directed mutagenesis or amino acid substitutions of EPSPS. However, the most glyphosate-resistant EPSPS enzyme to date has been isolated from Agrobacterium spp. strain CP4 and gives high levels of resistance in planta. Weeds resistant to glyphosate have offered further physiological mechanisms for glyphosate resistance. Resistant field bindweed had higher levels of 3-deoxy-d-arbino-heptulosonate 7-phosphate synthase, the first enzyme in the shikimate pathway, suggesting that increased carbon flow through the shikimate pathway can provide glyphosate resistance. Resistant goosegrass has reduced translocation of glyphosate out of the treated area. Although glyphosate resistance has been achieved by numerous mechanisms, currently the only independent physiological mechanism to give adequate and stable resistance to glyphosate for commercialization of glyphosate-resistant crops has been glyphosate-resistant forms of EPSPS.

Field experiments were conducted at Hays and Manhattan, KS, in 2002 and 2003 to determine winter wheat response to simulated drift rates of glyphosate and imazamox. Glyphosate and imazamox at 1/100×, 1/33×, 1/10×, and 1/3× of usage rates of 840 g ae/ha glyphosate and 35 g/ha imzamox were applied individually to wheat in the early jointing or the early flower stages of growth. Wheat injury and yield loss increased as herbicide rate was increased, with minimal effect from either herbicide at the 1/100× rate, and nearly complete kill and yield loss of wheat from both herbicides applied at the 1/3× rate, regardless of growth stage at application. In general, wheat injury and yield reduction were greater from glyphosate than from imazamox. In addition, wheat injury and yield loss were greater from herbicide treatment at the jointing stage than at the flowering stage of development. Correlation analysis suggests that visual injury is an accurate indicator of yield reductions. Germination tests of harvested grain showed that the viability of the wheat seed was not reduced if plants survived the herbicide treatment and produced a harvestable seed.

Dose-response experiments were conducted in a greenhouse on a biotype of johnsongrass from Washington County, Mississippi, to determine the level of resistance to the aryloxyphenoxypropionate (AOPP) herbicide fluazifop-P-butyl and the cyclohexanedione (CHD) herbicides clethodim and sethoxydim. Both seedling and rhizomatous plants were evaluated. Resistant/susceptible (R/S) ratios were calculated based on GR50 values (the rate required to reduce shoot dry biomass, expressed as a percent of the control, 50%). The GR50 values for the resistant and susceptible seedling plants were 110 and 10 g ai/ha for clethodim, 193 and 34 g ai/ha for fluazifop-P-butyl, and 265 and 48 g ai/ha for sethoxydim, resulting in R/S ratios of 11.0, 5.7, and 5.5, respectively. The GR50 values for the resistant and susceptible rhizomatous plants were 609 and 39 g/ha for clethodim, 657 and 29 g/ha for fluazifop-P-butyl, and 668 and 30 g/ha for sethoxydim, resulting in R/S ratios of 15.6, 22.7, and 22.3, respectively.

Volunteer potato is a perennial weed that is difficult to control in crop rotations. It was our objective to build a small, low-cost robot capable of detecting volunteer potato plants in a cornfield and thus demonstrate the potential for automatic control of this weed. We used an electric toy truck as the basis for our robot. We developed a fast row-recognition algorithm based on the Hough transform and implemented it using a webcam. We developed an algorithm that detects the presence of a potato plant based on a combination of size, shape, and color of the green elements in an image and implemented it using a second webcam. The robot was able to detect potatoes while navigating autonomously through experimental and commercial cornfields. In a first experiment, 319 out of 324 images were correctly classified (98.5%) as showing, or not showing, a potato plant. In a second experiment, 126 out of 141 images were correctly classified (89.4%). Detection of a potato plant resulted in an acoustic signal, but future robots may be fitted with weed control equipment, or they may use a global positioning system to map the presence of weed plants so that regular equipment can be used for control.

Recent shifts in herbicide use patterns can be attributed to rapid, large-scale adoption of glyphosate-resistant soybean and cotton. A dramatic increase in glyphosate use is the most obvious change associated with the adoption of glyphosate-resistant crops. Consequently, the diversity of herbicides used for weed management in these crops has declined, particularly in soybean. To date, the availability of glyphosate-resistant corn has limited the use of glyphosate in corn. While exploiting the benefits of glyphosate-resistant crops, many growers have abandoned the principles of sound weed and herbicide-resistance management. Instead of incorporating glyphosate into a resistance management strategy utilizing multiple herbicide sites of action, many growers rely exclusively upon glyphosate for weed control. Although it is difficult to establish a clear relationship between the adoption of glyphosate-resistant crops and changes in other crop production practices, the increase in no-till and strip-till production of cotton and soybean between 1995 and 2002 may have been facilitated by glyphosate-resistant crops.

Weed management is a major production issue facing otebo bean growers in Ontario. Field trials were conducted at six Ontario locations during a 2-yr period (2003 and 2004) to evaluate the tolerance of otebo bean to the preplant incorporated (PPI) application of EPTC at 4,400 and 8,800 g ai/ha, trifluralin at 1,155 and 2,310 g ai/ha, dimethenamid at 1,250 and 2,500 g ai/ha, S-metolachlor at 1,600 and 3,200 g ai/ha, and imazethapyr at 75 and 150 g ai/ha. EPTC, trifluralin, dimethenamid, and S-metolachlor applied PPI resulted in minimal (less than 5%) visual injury and with exception of the low rate of dimethenamid causing a 16% reduction in shoot dry weight and the high rate causing an 8% plant height reduction had no adverse effect on plant height, shoot dry weight, seed moisture content, and yield. Imazethapyr applied PPI caused up to 7% visual injury and reduced plant height, shoot dry weight, and yield 8, 18, and 12% at 75 g/ha and 19, 38, and 27% at 150 g/ ha, respectively. Seed moisture content was also reduced by 0.4% with both rates. Based on these results, otebo bean is not tolerant of imazethapyr applied PPI at rates as low as 75 g/ha, the proposed use rate. EPTC, trifluralin, dimethenamid, and S-metolachlor applied PPI have a 2× rate crop safety margin for use in otebo bean weed management.

Experiments were conducted to determine the inheritance of resistance in crosses between imazethapyr-resistant rice and red rice. Past experiments on red rice control, using the Clearfield rice technology, resulted in outcrossing between Clearfield rice and Stuttgart strawhull red rice. The F2 generation of these spontaneous crosses were characterized with respect to inheritance of imazethapyr resistance, leaf color and leaf pubescence, and seed shattering, pubescence, color, and size. Agronomic traits of hybrids were also observed in relation to their parents. To determine the segregation of resistance among F2 phenotypes, the response of three- to four-leaf plants to imazethapyr was scored 3 wk after application as resistant (R, no imazethapyr symptoms), susceptible (S, death of plants), or intermediate (I, stunted plants). R, I, and S phenotypes segregated in a 1:2:1 ratio in the F2 generation. Two- or three-gene inheritance was documented for leaf and seed characteristics. A wide range in onset of flowering (70 to 130 d after planting) was observed in F2 families, although 6% of the plants did not flower during the growing season. F2 plants were taller and had more tillers than any of their parents. Resistance to imazethapyr is associated with a single, incompletely dominant allele.

Populations of eastern black nightshade suspected of being resistant to acetolactate synthase (ALS) inhibitors have been reported since 1999 in different locations in Ontario, Canada. This event has threatened the use of ALS inhibitors for control of this species. The objectives of this study were to evaluate the spectrum of resistance to different ALS-inhibiting herbicides and to examine the effectiveness of alternative modes of action herbicides. Growth room experiments were conducted to determine the response to imazethapyr and atrazine in seven suspected ALS inhibitor– resistant populations. One resistant and one susceptible population were further characterized for their response to ALS inhibitors and chloroacetamides. Seven populations were able to survive imazethapyr at 100 g ai/ha, while there was no resistance to atrazine. Compared to a susceptible (S) population, resistant (R) population SOLPT 1 had 726-, 31-, 6-, and 4-fold resistance to postemergence (POST) applied imazethapyr, imazamox, primisulfuron, and flumetsulam, respectively. Preemergence (PRE) application of imazethapyr, flumetsulam, cloransulam, nicosulfuron, prosulfuron, and rimsulfuron did not provide control of the R population, whereas they totally controlled the S population. The chloroacetamide herbicides metolachlor, dimethenamid, and flufenacet all provided at least 90% control of both R and S populations when applied PRE at the recommended field rates. The ALS inhibitors will not provide adequate control of these resistant populations, but acceptable control could be achieved with chloroacetamides or with atrazine.

Perennial pepperweed is an invasive weed spreading rapidly throughout a wide range of habitats in the western United States. This study was designed to test whether integrating early season mowing with a systemic herbicide application would improve the control of perennial pepperweed. Experiments were conducted within three different environments including high desert, roadside, and floodplain areas. In treatments not mowed, chlorsulfuron at 0.104 kg ai/ha reduced perennial pepperweed biomass and density by 100%, whereas applications of 2,4-D at 2.11 kg ae/ha and glyphosate at 3.33 kg ae/ha were inconsistent and did not provide effective control. Mowing alone did not reduce perennial pepperweed biomass or density the following year, but mowing followed by application of a herbicide treatment to resprouting plants reduced biomass at all three sites compared with nonmowed treatments including herbicides. Among combinations of mowing and herbicides, chlorsulfuron at 0.052 kg/ha reduced perennial pepperweed biomass by >99% at all three sites, and glyphosate at 3.33 kg/ha reduced biomass by ≥80% at two sites 1 yr after applications. The effectiveness of glyphosate at 3.33 kg/ha in combination with mowing provides land managers with a valuable control option for perennial pepperweed in wetland or riparian areas where chlorsulfuron is not registered for use.