Durum wheat culture requires a high level of N fertilization to achieve ideal protein concentration for semolina and pasta quality, contributing to N losses. Optimizing plant N use efficiency could improve agro-environmental balance. In the current paper, we studied the impact of the marine (DPI4913) and fungal (AF086) extracts (biostimulants) applied on leaves on growth, N absorption and N fluxes in durum wheat in field and greenhouse experiments. In the field, 15NO3− and 15NH4+ were injected into the soil; in the greenhouse, N of the flag-leaf was labelled with 15NH4+. Flag-leaf senescence was studied by estimating leaf chlorophyll concentration. In greenhouse, biostimulants increased grain yield, total N in plant and the proportion of plant N in ears. When water was limited in greenhouse experiment, neither biostimulants had any effect. In the field, DPI4913 increased soil fertilizer-derived 15N accumulated in grains. In the greenhouse, biostimulants increased the proportion of 15N applied to the flag-leaf recovered in grains and accelerated leaf senescence. For plants treated with biostimulants, flag-leaf N resorption increased. Biostimulants had a larger positive impact on mineral N root uptake than on N remobilization. In conclusion, our study has shown that DPI4913 and AF086 can promote plant growth and grain yield, N uptake and remobilization. Thus, these biostimulants could be used to optimize durum wheat N fertilization and contribute to reduced N losses.