Starting microalgae continuous culture generally includes a preliminary batch culture to obtain sufficient cell density. It is possible to apply continuous regime from the beginning of the culture (IC mode) rather than to begin by an initial batch (IB mode). It is our purpose to check that both initial modes lead to identical steady-state cell characteristics. The microalga Isochrysis galbana affinis Tahiti was used for this comparative study. With an initial cell density of 5 × 105 cell ml–1 and a dilution rate of about 1.0 d–1, both IB and IC modes led to identical cell density once steady-state is reached between 6 and 8 d after inoculation in the two cases. Cell concentration of chlorophyll a and pheopigment a were found to be similar for IB and IC modes at steady-state. Initial culture conditions did not influence saturation irradiance and oxygen consumption rate, which were found to be 650 ± 143 μmol phot m–2 s–1 and 1.54 × 10–3 ± 10–5 μmol O2 ml–1 min–1, respectively. At steady-state, nutrient cell uptakes were ρN = 83.3 ± 2.0 fmol N cell–1 d–1 for NO3– and ρP = 5.5 ± 0.4 fmol P cell–1 d–1 for H2PO4– and did not exhibit significant differences between IB and IC modes. Under the prevailing experimental conditions, results show that IC mode involved very similar steady-state cell characteristics when compared to IB mode subsequent steady-state. IC mode could be an attractive alternative especially for experimental laboratory studies, as it should lead to higher flexibility in starting continuous cultures.