To validate carboxyterminal processing protease of D1 protein (CtpA) as a target for herbicide discovery, CtpA sense mRNAs were overexpressed to suppress the internal level of CtpA protein in mouseearcress plants. Using antibodies raised against recombinant CtpA protein, we demonstrated that we have generated transgenic mouseearcress plants with reduced levels of CtpA protein and plants with elevated levels of CtpA protein. Transgenic plants with severely reduced levels of CtpA protein exhibited a bleached and chlorotic phenotype and stunted growth. The mutant phenotypes were enhanced by bright illumination. However, plants with a slight reduction of CtpA protein did not exhibit the mutant phenotype and could not be distinguished from wild-type plants under normal growth conditions. Several CtpA enzyme inhibitors were shown to have herbicidal activity in planta. Interestingly, plants producing excessive amount of CtpA protein were shown to be resistant to these inhibitors. Our results suggest that CtpA is essential for plant growth and development, but a reduced amount of CtpA is sufficient to carry out its essential function. CtpA may be a good target for herbicide development, but very high levels of inhibition may be required to produce a herbicidal effect. In addition, overexpressing CtpA in target plants might provide a mechanism for producing plants resistant to the herbicide.

The objective of this study was to investigate the resistance level of Japanese foxtail to haloxyfop, an acetyl coenzyme A carboxylase (ACCase; EC 6.4.1.2)–inhibiting herbicide. Eleven biotypes were collected from oilseed rape fields in different areas in Jiangsu and Anhui provinces where haloxyfop had been continuously applied for various periods. Biotypes were assessed by two different methods, a seed bioassay and whole-plant assay, to identify the most resistant and susceptible biotypes for further studies on the activity of the target enzyme ACCase. A good correlation was obtained between the two different bioassay methods. The Jurong and Chuzhou biotypes were the most resistant and susceptible biotypes, respectively, whereas the other nine biotypes showed variable and relatively low degrees of haloxyfop resistance. Furthermore, target-site enzyme sensitivity results confirmed that the Jurong biotype was resistant to haloxyfop with a concentration of herbicide causing 50% inhibition of ACCase activity (IC50) of 9.19 µM, whereas the IC50 of the susceptible biotype (Chuzhou) was 0.76 µM, giving a resistance index of 12.

Experiments were conducted to evaluate a biotype of smooth pigweed that had survived applications of sulfonylurea (SU) and imidazolinone (IMI) herbicides in a single season. The source field had a history of repeated acetolactate synthase (ALS)-inhibiting herbicide use over several years. Whole-plant response experiments evaluated the resistant (R11) biotype and an ALS-inhibitor susceptible (S) smooth pigweed biotype to herbicides from the SU, IMI, pyrimidinylthiobenzoate (PTB), and triazolopyrimidine sulfonanilide (TP) chemical families. The R11 biotype exhibited 60- to 3,200-fold resistance to all four ALS-Inhibiting herbicide chemistries compared with the S biotype. Nucleotide sequence comparison of ALS genes from R11 and S biotypes revealed a single nucleotide difference that resulted in R11 having an amino acid substitution of aspartate to glutamate at position 376, as numbered relative to the protein sequence of mouseearcress. This is the first report of an amino acid substitution at this position of an ALS gene isolated from a field-selected weed biotype. To verify the role of this mutation in herbicide resistance, the ALS gene was cloned and expressed in Arabidopsis. Transgenic Arabidopsis expressing this ALS gene exhibited resistance to SU, IMI, PTB, TP, and sulfonylaminocarbonyltriazolinone ALS-Inhibiting herbicide classes.

Experiments were conducted to examine the utility of a spectrophometric leaf disc assay for detecting shikimate accumulation after glyphosate application in sunflower, proso millet, and wheat. The assay was conducted on both greenhouse- and field-grown plants. Glyphosate was applied at five rates ranging from 840 to 53 g ae ha−1. Shikimate accumulation data were generated at 1, 4, 7, and 14 d after application (DAA). Sunflower accumulated shikimate more rapidly and at lower glyphosate rates than the other two species. At 14 DAA, glyphosate at the two highest rates remained detectable in all three species. Plants receiving lower glyphosate doses (210, 105, and 53 g ae ha−1) had begun to grow out of the injury, or at least the shikimate levels in the plants were no longer significantly different than that present in the untreated controls. This spectrophotometric assay is both rapid and simple, with respect to other means of detecting shikimate, and it can be used to detect glyphosate drift. For it to be used by crop managers, samples from potentially drift-affected crops should be taken as soon as possible after the suspected drift event or immediately after the appearance of glyphosate injury.

Bispyribac is registered for postemergence control of broadleaf, sedge, and grass weeds in rice. Bispyribac inhibits the acetolactate synthase enzyme in sensitive plants. Herbicides in this class of chemistry require a spray adjuvant to achieve optimal efficacy, often achieve different levels of weed control according to the spray adjuvant used, and typically have rainfast periods of at least 6 to 8 h. Efficacy and rainfastness of bispyribac can be affected by spray adjuvant and the addition of urea ammonium nitrate (UAN). Greenhouse experiments were conducted to investigate the effect of spray adjuvant type, addition of UAN, and soil moisture on bispyribac efficacy on barnyardgrass. Control of barnyardgrass was improved when UAN was added to bispyribac at 0.4 or 0.8 g ha−1 plus an organosilicone-based nonionic surfactant (OSL/NIS) or methylated seed oil/organosilicone (MSO/OSL) spray adjuvant. The type of adjuvant added to the spray solution affected bispyribac efficacy on barnyardgrass. The addition of UAN decreased the rainfast period from 8 h (registered rainfast period) to 1 or 4 h (99 to 100% control) when either the OSL/NIS or MSO/OSL adjuvant was applied with bispyribac, respectively. Applying UAN and OSL/NIS or MSO/OSL adjuvant with bispyribac enhanced efficacy and reduced the time period required between bispyribac application and washoff during a rainfall event. Increasing soil moisture conditions resulted in greater efficacy from bispyribac when applied with and without UAN.

Common groundsel is an alien annual weed that has become increasingly troublesome in many crops in Ohio. Understanding the periodicity of seedling emergence and longevity of seeds buried in the soil may help growers devise more efficient strategies to control common groundsel. Studies were conducted to determine the dormancy status of common groundsel seeds over 24 mo, and to describe the effect of tillage and fertilizer on the pattern of seedling emergence and the rate of depletion of seeds from the soil seed bank. Common groundsel seeds were collected (June 2000 and 2002) from sites along a 700-km transect from Kentucky to Michigan (39°1′ and 43°36′N, respectively). Seeds were cleaned and placed in nylon mesh bags for burial in a common garden. Every month for the following 24 mo, replicate bags from each location were exhumed. Germination was tested under alternating temperatures of 20 and 10 C, for 14 and 10 h day/night, respectively. Germination response at each sampling date was similar regardless of seed source, but differed for the 2000 to 2002 and 2002 to 2004 experiments. Laboratory germination of seeds buried was initially high (98%) and declined rapidly to about 20% by midwinter. Germination increased to about 60% during the second summer, followed by a slow decline to 40% during winter and another rapid decline before the third summer. The rapid declines in germination were preceded by low soil temperatures (<5 C) and the germination peaks corresponded with periods of high soil temperatures (∼ 20 C). Results suggested that common groundsel follows a cycle of dormancy and nondormancy corresponding to decreases and increases, respectively, in soil temperature. During 2 yr of deep burial in undisturbed soil, 94% of the seeds germinated or died, suggesting that common groundsel seeds may not persist more than a few months in regularly disturbed soils.

Prohexadione calcium is an experimental turfgrass growth regulator that selectively controls or suppresses annual bluegrass in desirable turfgrass such as creeping bentgrass, Kentucky bluegrass, and perennial ryegrass. To help explain interspecific differences in turfgrass and weed response to prohexadione calcium, two laboratory trials were conducted to measure 14C-prohexadione calcium absorption, translocation, and metabolism in these four species. Annual and Kentucky bluegrass absorbed more prohexadione calcium than creeping bentgrass and perennial ryegrass when averaged over harvest timing and trial. Radioactivity partitioning to other foliage did not differ between species but annual bluegrass and Kentucky bluegrass translocated more radioactivity to roots and evolved more radioactive CO2 than creeping bentgrass and perennial ryegrass. Thin-layer chromatographic separations indicate radioactivity was translocated predominately as prohexadione calcium. When averaged over species and trial, 25 and 16% of recovered prohexadione calcium was metabolized within 1 h after treatment in treated leaves and other foliage, respectively. The rate of metabolic degradation was 0.7% h−1 in treated leaves and 0.4% h−1 in other foliage. Previous research indicates that annual and Kentucky bluegrass growth is suppressed more by prohexadione calcium than is growth of creeping bentgrass and perennial ryegrass. Increased prohexadione calcium absorption partially explained these interspecific differences in growth suppression.

Inconsistent control of barnyardgrass with bispyribac may be alleviated through adjuvant technology. Experiments were conducted to determine the effect of adjuvant and urea ammonium nitrate (UAN) on absorption and translocation of bispyribac in barnyardgrass. Additional experiments were conducted to determine when maximum absorption and translocation occurred with the use of a methylated seed oil/organosilicone adjuvant (MSO/OSL) plus UAN (0.37 L ha−1 and 2% v/v). In the initial experiment, 14C-bispyribac–treated leaves, nontreated leaves, and roots were collected 6 and 24 h after application. Absorption was greatest with tank-mixed MSO/OSL (0.37 L ha−1) plus UAN (2% v/v) and the proprietary blend of MSO/OSL/UAN (2% v/v) at 80 and 74% of applied 14C-bispyribac, respectively. Translocation to nontreated leaves and roots was also highest with these treatments. Increased translocation appeared to be due to greater herbicide absorption, not an increase in translocation rate. The addition of 32% UAN to MSO/OSL and nonionic organosilicone (OSL/NIS) adjuvant systems resulted in a four to fivefold increase in absorption compared with treatments without UAN. Recovery of 14C-bispyribac in additional experiments generally decreased as time after application increased; however, recovery was 86% or greater for all time intervals. By 12 h after application, 68% of applied 14C-bispyribac was absorbed. At this time, 14C-bispyribac was partitioned within the plant in the following manner: 48% in the treated area, 10% in leaf tissue from treated area to tip of the treated leaf, 1.9% in leaf tissue from treated area to collar region of the treated leaf, 1.6% in remaining leaves from collar of treated leaf upward, 5.3% in remaining leaves from collar of treated leaf downward to soil line, and 0.7% in the roots. These data indicate that maximum absorption was achieved within 12 h with a tank mix of MSO/OSL and UAN or the MSO/OSL/UAN blend.

In spring 2005, a downy brome population with possible resistance to fluazifop-P, an acetyl-CoA carboxylase (ACCase) inhibitor (group 1) herbicide was found in a commercial creeping red fescue seed production field, near La Grande, OR, where fluazifop-P had been used to control downy brome repeatedly over 7 yr. Greenhouse experiments were conducted to confirm resistance to a number of group 1 herbicides. The suspected resistant downy brome accession was tested for resistance to fluazifop-P and tested for cross-resistance to other aryloxyphenoxy propionate (APP) and cyclohexanedione (CHD) herbicides, including quizalofop-P, sethoxydim, and clethodim. Data recorded included plant-mortality counts and biomass. Tests revealed that the La Grande downy brome accession was highly resistant to fluazifop-P and sethoxydim at all tested rates. The La Grande accession suffered no mortality from fluazifop-P or sethoxydim treatments up to the maximum tested rate of eight times (8×) the labeled recommendation. The La Grande accession was resistant to quizalofop-P and clethodim at the labeled rate or less but was susceptible to application rates higher than the labeled rate. The control downy brome accession was completely susceptible to fluazifop-P, quizalofop-P, and clethodim at all rates and exhibited increasing susceptibility with increasing sethoxydim rate. This pattern of cross-resistance differs from that of a previously reported case of ACCase resistance in downy brome.

The effect of formulation and adjuvants on absorption and translocation of 14C-clethodim was determined at 1, 4, 12, 24, 48, and 72 h after treatment (HAT) in bermudagrass under greenhouse conditions. Absorption of 14C-clethodim with the 0.12 kg L−1 (15 to 85%) formulation was higher than with the 0.24 kg L−1 (5 to 40%) formulation, regardless of presence or absence of adjuvant. There was considerable variation in the effect of adjuvant on 14C-clethodim absorption. When either ammonium sulfate (AMS) or AMS plus crop oil concentrate (COC) was added to the 0.12 kg L−1 formulation, 14C-clethodim absorption increased significantly at all harvest times except at 12 HAT compared with 0.12 kg L−1 formulation alone, whereas, 14C-clethodim absorption after addition of COC to the 0.12 kg L−1 formulation was similar to the 0.12 kg L−1 formulation alone up to 24 HAT. Conversely, COC enhanced 14C-absorption at all harvest times when added to 0.24 kg L−1 formulation. Most of 14C-clethodim (79 to 100% of absorbed) remained in the treated leaf, independent of formulation or adjuvant. Formulation did not have an impact on distribution of absorbed 14C-clethodim; however, presence of an adjuvant increased movement of 14C-clethodim out of treated leaf. Of the absorbed 14C-label, most remained in the treated leaf. 14C-clethodim that translocated out of the treated leaf remained in the shoot, and negligible amount of 14C-clethodim translocated to roots. These results demonstrated improved absorption of clethodim with formulations containing half the active ingredient (0.12 kg L−1) and inclusion of both AMS and COC.

Because of its ability to induce contact dermatitis, the establishment and spread of poison ivy is recognized as a significant public health concern. In the current study, we quantified potential changes in the biomass and urushiol content of poison ivy as a function of incremental changes in global atmospheric carbon dioxide concentration (CO2). We also examined the rate of new leaf development following leaf removal to simulate responses to herbivory as functions of both CO2 and plant size. The experimental CO2 values (300, 400, 500. and 600 µmol mol−1) corresponded approximately to the concentration that existed during the middle of the 20th century, the current concentration and near and long-term projections for this century (2050 and 2090), respectively. Over 250 d, increasing CO2 resulted in significant increases in leaf area, leaf and stem weight, and rhizome length relative to the 300 µmol mol−1 baseline with the greatest relative increase occurring from 300 to 400 µmol mol−1. There was a nonsignificant (P = 0.18) increase in urushiol concentration in response to CO2; however, because of the stimulatory effect of CO2 on leaf biomass, the amount of urushiol produced per plant increased significantly for all CO2 above the 300 µmol mol−1 baseline. Significant increases in the rate of leaf development following leaf removal were also observed with increasing CO2. Overall, these data confirm earlier, field-based reports on the CO2 sensitivity of poison ivy but emphasize its ability to respond to even small (∼ 100 µmol mol−1) changes in CO2 above the mid-20th century carbon dioxide baseline and suggest that its rate of spread, its ability to recover from herbivory, and its production of urushiol, may be enhanced in a future, higher CO2 environment.

Genomics programs in the weed science community have not developed as rapidly as that of other crop, horticultural, forestry, and model plant systems. Development of genomic resources for selected model weeds are expected to enhance our understanding of weed biology, just as they have in other plant systems. In this report, we describe the development, characteristics, and information gained from an expressed sequence tag (EST) database for the perennial weed leafy spurge. ESTs were obtained using a normalized cDNA library prepared from a comprehensive collection of tissues. During the EST characterization process, redundancy was minimized by periodic subtractions of the normalized cDNA library. A sequencing success rate of 88% yielded 45,314 ESTs with an average read length of 671 nucleotides. Using bioinformatic analysis, the leafy spurge EST database was assembled into 23,472 unique sequences representing 19,015 unigenes (10,293 clusters and 8,722 singletons). Blast similarity searches to the GenBank nonredundant protein database identified 18,186 total matches, of which 14,205 were nonredundant. These data indicate that 77.4% of the 23,472 unique sequences and 74.7% of the 19,015 unigenes are similar to other known proteins. Further bioinformatics analysis indicated that 2,950, or 15.5%, of the unigenes have previously not been identified suggesting that some may be novel to leafy spurge. Functional classifications assigned to leafy spurge unique sequences using Munich Information Center for Protein or Gene Ontology were proportional to functional classifications for genes of arabidopsis, with the exception of unclassified or unknowns and transposable elements which were significantly reduced in leafy spurge. Although these EST resources have been developed for the purpose of constructing high-density leafy spurge microarrays, they are already providing valuable information related to sugar metabolism, cell cycle regulation, dormancy, terpenoid secondary metabolism, and flowering.

Weed genetic diversity is important for understanding the ability of weeds to adapt to different environments and the impact of herbicide selection on weed populations. Genetic diversity within and among six wild oat populations in China varying in herbicide selection pressure and one population in North Dakota were surveyed using 64 polymorphic alleles resulting from 25 microsatellite loci. Mean Nei's gene diversity (h) for six wild oat populations from China was between 0.17 and 0.21, and total diversity (HT) was 0.23. A greater proportion of this diversity, however, was within (Hs = 0.19) rather than among (Gst = 0.15) populations. For the wild oat population from the United States, h = 0.24 and HT = 0.24 were comparable to the values for the six populations from China. Cluster analysis divided the seven populations into two groups, where one group was the United States population and the other group included the six Chinese populations. The genetic relationships among six populations from China were weakly correlated with their geographic distribution (r = 0.22) using the Mantel test. Minimal difference in gene diversity and small genetic distance (Nei's distance 0.07 or less) among six populations from China are consistent with wide dispersal of wild oat in the 1980s. Our results indicate that the wild oat populations in China are genetically diverse at a level similar to North America, and the genetic diversity of wild oat in the broad spatial scale is not substantially changed by environment, agronomic practices, or herbicide usage.

Hydrilla is one of the most serious aquatic weed problems in the United States, and fluridone is the only U.S. Environment Protection Agency (USEPA)–approved herbicide that provides relatively long-term systemic control. Recently, hydrilla biotypes with varying levels of fluridone resistance have been documented in Florida. One susceptible and five fluridone-resistant biotypes of hydrilla varying in resistance levels were maintained in 950-L tanks under ambient sunlight and day-length conditions from September 2004 to September 2005 in absence of fluridone. Because fluridone is an inhibitor of the enzyme phytoene desaturase (PDS), the gene for PDS (pds) was cloned from fluridone-susceptible and -resistant hydrilla biotypes. Somatic mutations in amino acid 304 of hydrilla PDS are known to confer herbicide resistance. We determined pds sequence from these hydrilla biotypes at planting and 12-mo after planting. Two independent mutations at the arginine 304 codon of pds were found in the resistant hydrilla plants. The codon usage for arginine 304 is CGT, and a single point mutation yielding either serine (AGT) or histidine (CAT) was identified in different resistant hydrilla biotypes. There were no differences at codon 304 in the PDS protein of any hydrilla biotype 12-mo after planting. Several other mutations were also found in resistant pds alleles, though their possible role in herbicide resistance is unclear.

Microarray analysis was used to identify changes in gene expression in velvetleaf that result from competition with corn. The plants were grown in field plots under adequate N (addition of 220 kg N ha−1) to minimize stress and sampled at the V6 growth stage of corn (late June). Leaf area, dry weight, and N and P concentration were similar in velvetleaf plants grown alone or with corn. Competition, however, did influence velvetleaf gene expression. Genes involved in carbon utilization, photosynthesis, red light signaling, and cell division were preferentially expressed when velvetleaf was grown in competition with corn. A less clear picture of the physiological impact of growth in monoculture was provided by the data. However, several genes involved in secondary metabolism and a gene preferentially expressed in response to phosphate availability were induced. No differences were observed in genes responsive to water stress or sequestering/transporting micronutrients.

A hand-held hyperspectral radiometer was used to measure differences in reflectance characteristics of 24 Palmer amaranth and 15 pitted morningglory accessions collected from the central and southern United States. A hyperspectral reflectance reading was collected from two mature leaves at 24 and 27 d after emergence (DAE) for each accession. Two analysis techniques, linear discriminant analysis and best spectral-band combination (BSBC) analysis, were performed using various vegetation indices, spectral bands, and individual wavelengths. Differentiation of individual accessions was difficult. Palmer amaranth accession classification accuracies were < 50% using both analysis techniques, except one accession collected in South Carolina (63%), when pooled over data acquisition dates. Pitted morningglory accession classification accuracies were also generally < 50%. Classification accuracies were higher using BSBC analysis at 24 DAE; however, at 27 DAE only one accession resulted in classification accuracy > 30%. These results suggest there are only slight reflectance differences within Palmer amaranth and pitted morningglory accessions. These differences may not be predictable based upon accession origin because of the genetic diversity of Palmer amaranth and pitted morningglory. However, differentiation between Palmer amaranth and pitted morningglory was 100%. Thus, spectral sensors used to differentiate between Palmer amaranth and pitted morningglory do not need to be calibrated for a particular region of the United States, and differentiation between these two species can be made using reflectance characteristics.

Feral rye is an agricultural and ruderal weed of the western United States. We investigated how it has phenotypically diverged from its cultivated ancestor, domesticated cereal rye, and across its range since the introduction of its progenitor. Vegetative growth, flowering phenology, and reproductive characters of feral populations from across a 13° range in latitude in the northwestern United States were compared to that of rye cultivars under both vernalized (cold-treated) and nonvernalized conditions. Feral populations as a whole had smaller seeds, thinner culms, and a delay in flowering relative to cultivars, regardless of cold treatment. Vernalized feral populations from northern latitudes (northern California and eastern Washington) produced more, but smaller leaves and more tillers than both vernalized rye cultivars and southern California feral populations. Northern feral populations also flowered significantly later, irrespective of vernalization treatment. We conclude that feral rye is phenotypically distinct from domesticated cereal rye and that feral populations have diverged regionally from one another. Reproductive isolation from domesticated rye, due both to the loss in popularity of the crop and to phenological shifts in feral rye relative to cultivars, may be contributing to the rapid evolution of this weed away from its domesticated ancestor in less than 120 yr since its introduction.

Hydrilla is one of the most serious aquatic weed problems in the United States, and fluridone is the only herbicide approved by the U.S. Environment Protection Agency that provides systemic control. Recently, hydrilla biotypes with varying levels of fluridone resistance have been documented in Florida. Hydrilla biotypes of varying fluridone resistance levels were maintained in 900-L tanks under natural atmospheric conditions from September 2004 to September 2005 in the absence of fluridone. Hydrilla shoot tips were collected from each biotype during September 2004 (at planting), December 2004 (3 mo after planting [MAP]), March 2005 (6 MAP), June 2005 (9 MAP), and September 2005 (12 MAP) and exposed to 5, 10, 15, 20, 30, and 50 µg L−1 fluridone to assess changes in susceptibility to this herbicide over time. Nonlinear regression analysis was used to calculate EC50 values for phytoene and β-carotene (effective fluridone concentration to increase/decrease the phytoene/β-carotene content in hydrilla plant tissue by 50% over the untreated control) at each time interval. EC50 values did not change in the susceptible hydrilla biotype over time. The EC50 values for phytoene and β-carotene for the susceptible biotype were 7.5 and 8.9 µg L−1, respectively, at planting and 7.6 and 9.4 µg L−1, respectively, at 12 MAP. Resistant hydrilla biotypes (R1–R5) also showed no change in EC50 phytoene values over time. Although, EC50 β-carotene values in resistant biotypes R1, R3, R4, and R5 did not change over time, R2 recorded a reduction in EC50 β-carotene at 12 MAP. Also, a 0.5-point decrease in resistance factor was observed for all resistant biotypes. Future long-term studies are needed to evaluate stability of resistant hydrilla biotypes in the absence of fluridone selection pressure.

Giant ragweed is a competitive, allergenic weed that persists in agricultural fields and early successional sites. Field experiments were conducted to determine the effects of seed size and seed burial depth on giant ragweed emergence and seed demise. In a seedling emergence experiment, small (< 4.8 mm in diameter) and large (> 6.6 mm in diameter) seeds were buried 0, 5, 10, and 20 cm in fall 1997, and weed emergence was monitored over the next seven growing seasons. A generalized linear mixed model fit to the cumulative emergence data showed that maximum emergence for both seed sizes occurred at the 5-cm burial depth, where probability of emergence was 19% for small seeds and 49% for large seeds. Emergence probability at the 10-cm burial depth was 9% for small seeds and 30% for large seeds, and no seedlings emerged from the 20-cm burial depth. The model predicted that ≥ 98% of total cumulative emergence was completed after four growing seasons for large seeds buried 5 cm, five growing seasons for small seeds buried 5 cm and large seeds buried 10 cm, and seven growing seasons for small seeds buried 10 cm. Seed size and burial treatment effects on seed demise were tested in a second experiment using seed packets. Rates of seed demise were inversely proportional to burial depth, and the percentage of viable seeds remaining after 4 yr ranged from 0% on the soil surface to 19% at the 20-cm burial depth. Some seeds recovered from the 20-cm burial depth were viable after 9 yr of burial. These results, coupled with previous research, suggest that seed size polymorphism facilitates giant ragweed adaptation across habitats and that a combination of no-tillage cropping practices, habitat modification, and timely weed control measures can reduce its active seed bank in agricultural fields by 90% or more after 4 yr.

In a survey of herbicide responses among Illinois waterhemp half-sib populations, several were observed with differential responses to imazethapyr and thifensulfuron, two acetolactate synthase (ALS)–inhibiting herbicides. Plants from two waterhemp populations were verified resistant to imazethapyr, but susceptible to chlorimuron, using a nondestructive leaf-disc assay. Sequencing of the ALS gene revealed that imazethapyr-resistant waterhemp plants from both populations had inferred amino acid substitutions at position 653 of ALS. Depending on the population, the serine at position 653 of ALS was substituted with either asparagine (S653N) or threonine (S653T). Waterhemp lines were derived from each population to create uniformly imidazolinone-resistant (IR) waterhemp biotypes, designated IR-62 and IR-101. ALS-inhibitor responses of each IR biotype were compared with a previously identified ALS inhibitor–resistant biotype with a tryptophan to leucine substitution at position 574 (W574L) and an herbicide-susceptible control. Whole-plant dose–response experiments with waterhemp biotypes containing W574L, S653N, or S653T mutations indicated that each biotype was resistant to imazethapyr, but only the biotype with a W574L mutation was resistant to thifensulfuron. In vitro ALS-activity assays revealed unique patterns of cross-resistance among protein extracts derived from each biotype in response to imazethapyr, thifensulfuron, cloransulam, and pyrithiobac. In conclusion, three different forms of target-site–based resistance to ALS inhibitors have been identified in waterhemp.