Products were collected from rhizospheres of a hydroponically grown sorghum-sudangrass hybrid [Sorghum bicolor (L.) Moench. × Sorghum sudanense (P.) Stapf. ‘DeKalb SX17+’] (SSH) and tested for effects on seedling root growth of selected species. Phytotoxic activity was recovered in CH2Cl2 extracts of aqueous solutions of the rhizosphere products. Significant inhibition of annual ryegrass (Lolium multiflorum L.) and alfalfa (Medicago sativa L. was produced by ca. 200 ppmw (H2O weight basis) of the most phytotoxic fraction in filter-paper-disk bioassays. In soils, 70 ppmw (soil weight basis) inhibited alfalfa but not ryegrass. Among several bioassay species, alfalfa was the most sensitive and curly dock (Rumex crispus L. ♯ RUMCR) the least. Treatment of SSH plants with herbicides increased the phytotoxicity but not the amounts of materials collected. Sethoxydim {2-[1-(ethoxyimino)-butyl]-5-[2-ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one} was detected in rhizosphere products from plants treated with the herbicide. Amounts of products collected from rhizospheres of common rye (Secale cereale L. ‘Wheeler’), johnsongrass [Sorghum halepense (L.) Pers. ♯ SORHA], and cucumber (Cucumis sativus L. ‘PI 169391’) were similar to those from SSH, but their phytotoxicities differed. Johnsongrass products were most phytotoxic, those from common rye and SSH were similar in phytotoxicity, and those from cucumber were least phytotoxic.

Distribution of 14C-glyphosate [N-(phosphonomethyl)glycine] in chemically treated and physically manipulated common milkweed [Asclepias syriaca (L.) ♯ ASCSY] was studied in greenhouse and growth chamber experiments. Pretreatment with glyphosate at 0.6 and 1.1 kg ae/ha 3 days prior to 14C-glyphosate application to leaves reduced the concentration of 14C recovered from shoots and leaves above the 14C-glyphosate-treated leaves but had no influence on the concentration of 14C in proximal root buds. Partial removal of the shoot and root prior to the application of 14C-glyphosate increased bud respiration and the concentration of 14C in the proximal root buds. Proximal root buds treated with 1 mM of 6-benzyl-aminopurine (BAP) for 6 days (3 days prior to application of 14C-glyphosate) contained seven times more 14C/mg than root buds of BAP-untreated plants, suggesting that dormant buds could be chemically stimulated to accumulate higher concentrations of glyphosate. BAP-treated buds were killed by foliar applications of glyphosate at 1.1 kg/ha, indicating that proximal root buds can be stimulated to acquire lethal concentrations of glyphosate.

The butyl ester of fluazifop {[(±)-2-[4-[[5-trifluoromethyl)-2-pyridinyl] oxy] phenoxy)propanoic acid} at 0.26 μM in nutrient solution inhibited root growth of hydroponically grown goosegrass (Eleusine indica Gaertn. ♯ ELEIN), large crabgrass [Digitaria sanguinalis (L.) Scop. ♯ DIGSA], and giant foxtail (Setaria faberi Herrm. ♯ SETFA). Treating the soil and plant foliage at 0.035 or 0.07 kg ai/ha did not result in greater phytotoxicity than exposing only the foliage of each grass to the herbicide. Foliar-applied fluazifop was retained on the foliage in similar amounts by each of the species. Translocation of 14C to all plant parts was detected 6 h after foliar application of the butyl ester of 14C-fluazifop to the grasses in the pretillering or tillering stage. The majority (90%) of 14C absorbed by each of the species remained in the treated leaf. In hydroponic studies, each species exuded 14C into nutrient solution following foliar application of the 14C-labeled herbicide. The exuded material was predominantly fluazifop with small amounts of compounds more polar than the butyl ester of fluazifop. Uptake and translocation studies suggest that the greater sensitivity of goosegrass to fluazifop may be related to higher concentrations of the herbicide present in plant tissue.

Field experiments established that tetraploid soybean [Glycine max (L.) Merr.] plants were relatively tolerant while diploid plants were highly susceptible to metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one] applied both preemergence and postemergence. Sensitivity of diploids and tolerance of tetraploids was also found in growth chamber experiments. Autoradiographs prepared 4 days after 14C-metribuzin application showed a high level of 14C-translocation to all parts of diploid plants including meristems. In contrast, only low levels of 14C were translocated in tetraploid plants, with no 14C-movement into meristems. Tetraploid plants rapidly transformed absorbed metribuzin to nontoxic products. Diploids were incapable of inactivating absorbed metribuzin at a rate sufficient to prevent injury. Differences in absorption, translocation, and metabolism of metribuzin appeared to be the main factors in the diploid and tetraploid differential response in field and growth chamber experiments. Differences in the rate of metribuzin metabolism appeared to be the factor responsible for the differential response in diploid and tetraploid cell suspension cultures. The primary polar metabolites were conjugates of metribuzin and deaminated metribuzin [6-tert-butyl-3-(methylthio)-1,2,4-triazin-5(4H)-one] with a ratio of 8:1, respectively.

Root absorption of 14C-5 (ring)-metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one] from hydroponic culture and its subsequent translocation in tolerant (‘Russet Burbank’) and susceptible (‘Chipbelle’) potato (Solanum tuberosum L.) cultivars were measured. Differences in root absorption did not appear to be as important to differential tolerance as did translocation differences. Total available metribuzin absorbed was 6 and 7%, 18 and 29%, and 31 and 45% after 1, 4, and 8 days for Russet Burbank and Chipbelle, respectively. Radioactivity was concentrated in stems, petioles, and leaf veins in the tolerant cultivar, whereas interveinal leaf tissue was the major accumulation site for 14C in the susceptible cultivar. Of the total radioactivity in the plants after 8 days, 82 and 87% were in the foliage for the tolerant and the susceptible cultivar, respectively. Translocation was apoplastic. Metribuzin tolerance by Russet Burbank is due in part to restricted translocation to the leaf blades. A model for differential transport between cultivars is proposed.

The influence of water stress on johnsongrass [Sorghum halepense (L.) Pers. ♯ SORHA] physiology was evaluated in a semiarid environment. Stomatal conductance of johnsongrass responded to more negative leaf water potential and increasing leaf temperature. The sensitivity of the leaf temperature effect was dependent on the soil water content. At low soil water content, conductance was limited by low water potential, and increasing leaf temperature had little effect. Conductance of CO2 was related to net photosynthesis in a curvilinear manner, with conductance levels greater than 0.3 mol·m-2· s-1 being in excess of that necessary for maximum photosynthesis. At both high conductance levels and low levels associated with increased water stress, intercellular CO2 concentration increased, indicating nonstomatal limitations to photosynthesis. Decreased osmotic potential provided the highest correlation with the linear decline of photosynthetic rate as stress intensified. The expression of osmotic adjustment in johnsongrass is reported during grain filling. Plants in the milkdough stage of grain filling had approximately 0.3 MPa lower osmotic potential at any relative water content than those at anthesis.

Brownspine pricklypear (Opuntia phaecantha Engelm. & Bigel.) was effectively controlled within 2 yr following application of a 1:1 mixture of 2,4,5-T [(2,4,5-trichlorophenoxy)acetic acid] and picloram (4-amino-3,5,6-trichloropicolinic acid) at a rate of 0.6 kg ae/ha. Brownspine pricklypear canopy cover and dry weight declined from approximately 23% and 3800 kg/ha to 8% and 1600 kg/ha, respectively. No significant difference in total herbaceous forage dry weight was found between plants growing inside brownspine pricklypear canopy areas and plants growing outside the canopy areas. Differences between areas in species composition were significant in that cool-season grasses dominated the canopy area of the brownspine pricklypear colonies while warm-season grasses dominated the area outside the canopy. Control of brownspine pricklypear will enhance livestock carrying capacity of rangeland in the Rolling Plains of Texas by increasing forage availability but not forage production.

Red rice (Oryza sativa L. ♯ ORYSA) densities of 5, 108, and 215 plants/m2 reduced grain yield of commercial rice (Oryza sativa L.) 22, 77, and 82%, respectively. At a cultivated rice density of 195 plants/m2, red rice at 5, 108, and 215 plants/m2 reduced straw dry weight of cultivated rice 18, 66, and 68%, respectively. At a red rice density of 5 plants/m2, reduction in number of cultivated rice grains per panicle ranged from 8 to 18%, whereas densities of 108 and 215 plants/m2 reduced grains per panicle 56 to 70%. Red rice grain yield was 24 to 33% lower in ‘Mars' rice than in ‘Lebonnet’. Mars, a medium-grain cultivar that matures in 138 days, competed better with red rice than Lebonnet, a long-grain cultivar that matures in 126 days.

Genetic variability for loci governing enzyme/morphological variants and for herbicide response was determined in 10 populations of the slender wild oat (Avena barbata Pott. ex Link ♯ AVEBA), six populations of wild oat (Avena fatua L. ♯ AVEFA), and three populations of godetia (Clarkia williamsonii Lewis & Lewis). The enzyme loci were identified by starch gel electrophoresis and included peroxidase, 6-phosphogluconate dehydrogenase, esterase, and leucine aminopeptidase for the slender wild oat; peroxidase, esterase, leucine aminopeptidase, and malate dehydrogenase for the wild oat; and esterase, phosphoglucoisomerase, leucine aminopeptidase, acid phosphatase, and glutamate oxaloacetate transaminase for godetia. Morphological loci included lemma and leaf sheath hairiness for the oats. For both the enzymatic and morphological loci, levels of genetic variation for each population were quantified by a polymorphic index. The herbicide barban (4-chloro-2-butynyl 3-chlorophenylcarbamate) was used on the wild oats; bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) was used on godetia. Genetic variation for herbicide response was based on genetic variances calculated from phytotoxicity scores. Populations were ranked from highest to lowest for the polymorphic indices and the genetic variances. Concordance between the rankings was tested by rank correlation. Statistically significant relationships were found between the enzyme/morphological characters and herbicide response in the slender wild oat and the wild oat. For some species, the level of genetic variation for response to herbicides appears to be associated with genetic variation for enzymatic and morphological loci.

Field experiments were conducted in 1979, 1980, and 1981 to determine the level of interference five broadleaf weed species exert on soybean [Glycine max (L.) Merr.]. Weed species studied were common cocklebur (Xanthium pensylvanicum Wallr. ♯ XANST), common ragweed (Ambrosia artemesiifolia L. ♯ AMBEL), common lambsquarters (Chenopodium album L. ♯ CHEAL), sicklepod (Cassia obtusifolia L. ♯ CASOB), and redroot pigweed (Amaranthus retroflexus L. ♯ AMARE). The following soybean seed yield reductions were observed with a density of 16 weeds/10-m row: redroot pigweed 22%, common lambsquarters 15%, common ragweed 12%, and sicklepod 5%, respectively. At a density of 8 weeds/10-m row, common cocklebur reduced soybean yield 11%. No single weed growth parameter predicted soybean seed yield reduction for all weed species. Soybean height was reduced by sicklepod competition; was not affected by competition from common lambsquarters, common ragweed, or common cocklebur; and was increased in the presence of redroot pigweed at 12 weeks after planting, when measured 30 cm from the weeds. Leaf area of soybean was higher at greater distances from the weed for all weed species. The range of soybean leaf area reductions occasioned by proximity to individual weed species corresponded fairly well with differences in soybean yield reduction.

The influence of acid scarification on the responsiveness of curly dock (Rumex crispus L. ♯ RUMCR) seeds to physical and chemical factors was studied. Scarification made the seeds responsive to GA4+7 (a mixture of gibberellin A4 + A7) and markedly improved the responsiveness to light, moist-chilling, and a 1-h 40-C temperature shift. Scarification reduced the mechanical restraint of the seedcoat by 0.4 to 0.8 MPa. Removal of the entire pericarp induced 69% germination in the dark. Moist-chilling treatment of seeds reduced the soaking period needed for maximum response to the high-temperature shift from 24 to 6 h. The embryo growth potential generated by moist-chilling was less in magnitude than that by the reduction in seed coat restraint by scarification. Induction of secondary dormancy was prevented to a marked degree in scarified (chilled or unchilled) seeds during polyethylene glycol-6000 (PEG) treatment in light or in the presence of GA4+7 while intact seeds rapidly became secondarily dormant in light. The ability of scarified seeds to remain germinable or nondormant for a longer period than the intact seed in low water potential imbibing medium may be due to a net reduction (by 0.4 to 0.8 MPa) in the impact on the embryo of germination-preventing forces (low water potential plus mechanical restraint of the seed coat).

Populations of large-flowered taxa of Carduus from many locations in the United States and Canada were grown in a common nursery from 1975 to 1983 at Lincoln, NE. Dates of first bloom and associated degree-day accumulation data exhibited some consistent relationships. Four species appeared to be represented in the collection. These included Carduus thoermeri Wein., C. macrocephalus Desf., and musk thistle (C. nutans L. ♯ CRUNU). A collection from British Columbia should be accorded species status but apparently has not been named in the literature. Interbreeding has taken place in the nursery and these combinations are intermediate between C. thoermeri and C. macrocephalus and between C. thoermeri and the British Columbia material. Some morphological characteristics supported these separations.

The influence of relative planting date on the growth of common cocklebur (Xanthium pensylvanicum Wallr. ♯ XANST), common ragweed (Ambrosia artemesiifolia L. ♯ AMBEL), sicklepod (Cassia obtusifolia L. ♯ CASOB), and redroot pigweed (Amaranthus retroflexus L. ♯ AMARE) grown in competition with soybean [Glycine max (L.) Merr. ‘Bragg’] was studied in the greenhouse. Increases in dry matter and height were slower for the five weed species than for soybean throughout the period of the study. The root: shoot ratio of soybean was the highest of any plant in the study, while common ragweed, common cocklebur, common lambsquarters, and sicklepod were intermediate, and redroot pigweed was the lowest. Soybean dry weight was always reduced when grown in competition with a weed. Soybean dry-matter production was reduced most when weeds were planted 2 weeks before soybean, especially with common cocklebur and common lambsquarters. Weed dry-matter content was severely reduced when the weed seed were planted simultaneously with or following soybean. Soybean height was usually reduced by competition with the weeds. The height of common ragweed was increased, however, when planted simultaneously with soybean. Common lambsquarters, redroot pigweed, and common ragweed heights were increased when planted 2 weeks prior to soybean.

Sethoxydim {2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one}, CGA-82725 {2-propynyl 2-[4-[(3,5-dichloro-2-pyridinyl)oxy]phenoxy]propanoate}, RO 13-8895 {acetone-O-[D-2-[p-(α,α,α-trifluoro-p-tolyl)oxy]phenoxy]propionyl oxime}, and difenopenten {4-[4-[4-(trifluoromethyl)phenoxy]phenoxy]-2-pentenoic acid} were evaluated as single and/or split applications for postemergence control of johnsongrass [Sorghum halepense (L.) Pers. ♯ SORHA] grown from rhizomes in soybeans [Glycine max (L.) Merr.]. Good to excellent control, crop safety, and yield enhancement were achieved with all herbicides evaluated. Antagonism between postemergence grass and broadleaf herbicides was evaluated by applying sethoxydim, CGA-82725, RO 13-8895, and difenopenten alone to soybeans infested with johnsongrass grown from seed, and applying these herbicides as mixtures with acifluorfen {5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid}, bentazon [3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide], or benazolin (4-chloro-2-oxobenzothiazolin-3-ylacetic acid). Acifluorfen was most antagonistic to the activity of postemergence grass herbicides, with difenopenten and sethoxydim the most susceptible to antagonism. Reductons in johnsongrass control were attributed to antagonism by broadleaf herbicides, which caused soybean yield reductions of up to 67%.

Weed control in no-till soybeans [Glycine max (L.) Merr. ‘Wells’] planted into shredded corn (Zea mays L.) residue was evaluated at Concord, NE, over a 3-yr period. Herbicide factors evaluated were time of herbicide application for no-till soybeans, efficacy of glyphosate [N-(phosphonomethyl)glycine] relative to paraquat (1,1’-dimethyl-4,4’-bipyridinium ion) for control of emerged weeds, and the efficacy of alachlor [2-chloro-2’,6’-diethyl-N-(methoxymethyl)acetanilide], metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide], oryzalin (3,5-dinitro-N4,N4-dipropylsulfanilamide), and pendimethalin [N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzenamine] for residual weed control. Combination residual and contact herbicide treatments were applied either as a preplant surface application or preemergence after no-till-planted soybeans. Removal of existing weed growth was not consistently better with the preplant surface application compared to later removal after soybean planting. Paraquat and glyphosate gave nearly equal control of emerged weeds. Addition of 2,4-D [(2,4-dichlorophenoxy)acetic acid] with paraquat in the tank mix did not improve weed control. Use of narrow, ripple coulters on the no-till planter resulted in minimal disturbance of the preplant surface herbicide so that additional preemergence herbicide at planting was seldom beneficial.

Herbicide treatments of tank mixtures or sequential applications were applied at different growth stages and compared with standard treatments for control of barnyardgrass [Echinochloa crus-galli (L.) Beauv. ♯ ECHCM], bearded sprangletop [Leptochloa fascicularis (Lam.) Gray ♯ LEFFA], and aquatic weeds in rice (Oryza sativa L. ‘Starbonnet’). Some herbicides injured rice more than the propanil (3’,4’-dichloropropionanilide) or molinate (S-ethyl hexahydro-1H-azepine-1-carbothioate) standards, but injury for all treatments was inconsequential. Postemergence tank mixture treatments that controlled barnyardgrass (two- to four-leaf stages), bearded sprangletop (two-leaf to tillering stages), and aquatic weeds better than standard treatments were propanil plus butachlor [N-(butoxymethyl)-2-chloro-2’,6,-diethylacetanilide], oxadiazon [2-tert-butyl-4(2,4-dichloro-5-isopropoxyphenyl)-δ2-1,3,4-oxadiazolin-5-one] thiobencarb {S-[(4-chlorophenyl)methyl]diethylcarbamothioate}, or pendimethalin [N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzenamine].

In greenhouse studies, the butyl ester of fluazifop {(±)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy]propanoic acid} applied preemergence at 0.035 kg ai/ha gave 91% control of goosegrass (Eleusine indica Gaertn. ♯ ELEIN), 79% control of large crabgrass [Digitaria sanguinalis (L.) Scop. ♯ DIGSA], and 73% control of giant foxtail (Setaria faberi Herrm. ♯ SETFA). In field studies, the butyl ester of fluazifop at 0.56 kg ai/ha applied preemergence gave 79% control of goosegrass, 76% control of large crabgrass, and 53% control of giant foxtail. The butyl ester of fluazifop at 0.07 kg/ha gave 79 to 85% control of each grass at the pretillering stage in field studies. The same rate applied at the early tillering stage gave 84% control of goosegrass but only 53 and 58% control of large crabgrass and giant foxtail, respectively. Relative species response was similar from spray and leaf-spot applications of the herbicide. The butyl ester of fluazifop at 4 μg/plant when spot-applied to leaves of the three grasses at the tillering stage resulted in 65% control of goosegrass but only 20 and 25% control of large crabgrass and giant foxtail, respectively.

Of 17 thiocarbamate herbicides tested in the greenhouse in repeat soil applications made 4 to 16 weeks apart, 9 showed definite losses in herbicidal activity after the second application. Those showing reduced activity included EPTC (S-ethyl dipropylthiocarbamate), vernolate (S-propyl dipropylthiocarbamate), and butylate (S-ethyl diisobutylthiocarbamate), which have been reported previously to develop accelerated breakdown, plus R-15574 (S-benzyl dipropylthiocarbamate and the sulfoxides of EPTC, vernolate, butylate, SC-7829 (S-propyl diisobutylthiocarbamate), and SC-8149 (S-butyl diisobutylthiocarbamate). Thiocarbamates that showed no significant reduction in activity after the second application were pebulate (S-propyl butylethylthiocarbamate), cycloate (S-ethyl N-ethylthiocyclohexanecarbamate), molinate (S-ethyl hexahydro-1H-azepine-1-carbothioate), R-1880 (S-ethyl dibutylthiocarbamate), R-1856 (S-tertiarybutyl dipropylthiocarbamate), R-1853 (S-isobutyl diethylthiocarbamate), R-1906 (S-butyl diisopropylthiocarbamate), and R-12001 [S-isopropyl 1-(5-ethyl-2-methyl)piperidine carbothioate]. Of 16 nonthiocarbamate herbicides tested, a reduction in control of weeds occurred after the second soil application with 2,4-D [(2,4-dichlorophenoxy)acetic acid], dalapon (2,2-dichloropropionic acid), chlorpropham (isopropyl m-chlorocarbanilate), propham (isopropyl carbanilate), TCA (trichloroacetic acid), pronamide [3,5-dichloro (N-1,1-dimethyl-2-propynyl)benzamide], napropamide [2-(α-naphthoxy)-N,N-diethylpropionamide], bensulide [O,O-diisopropyl phosphorodithioate S-ester with N-(2-mercaptoethyl)benzenesulfonamide], alachlor [2-chloro-2’,6’-diethyl-N-(methoxymethyl)acetanilide], and diethatyl [N-(chloroacetyl)-N-(2,6-diethylphenyl)glycine]. Losses with the latter five herbicides after repeat application have not been reported previously.

Field experiments were conducted to investigate methods of controlling red rice (Oryza sativa L. ♯ ORYSA) in drill-seeded rice (O. sativa). Treatments included the rice cultivar ‘Mars', coated with calcium peroxide (CaO2) at 40% (w/w) and a crop protectant, R-33865 (O,O-diethyl-O-phenyl phosphorothioate) at 0.5 and 1% (v/w). Molinate (S-ethyl hexahydro-1H-azepine-1-carbothioate) at 6.7 kg ai/ha was applied preplant incorporated (ppi). The land was flooded (2.5 to 5 cm deep) after seeding with rice (100 kg/ha, 2.5 cm deep), and the water was maintained throughout the growing season. CaO2, with or without molinate, increased rice grain yield 50% and increased rice culm density fivefold above untreated rice. Molinate applied ppi controlled 96% of the red rice. Rice seed coated with only CaO2 or with CaO2 plus R-33865 at 0.5%, each combined with ppi molinate, produced 5690 and 6030 kg/ha of grain, respectively. These high yields were associated with red rice control by molinate and good stands of rice provided by O2 supplied by CaO2. R-33865 applied to rice seed at 1% (v/w) injured rice by reducing rice culm densities 41%, compared with rice without protectant.

Terbacil (3-tert-butyl-5-chloro-6-methyluracil), diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea], hexazinone [3-cyclohexyl-6-(dimethylamino)-1-methyl-1,3,5-triazine-2,4(1H,3H)-dione], and metribuzin [4-amino-6-butyl-3-(methylthio)-as-triazin-5(4H)-one] controlled palmer amaranth (Amaranthus palmeri S. Wats. ♯ AMAPA) in established alfalfa (Medicago sativa L.) when applied after first harvest. However, these same herbicides did not control palmer amaranth when applied early-season in March. Alfalfa forage yields were increased when palmer amaranth was controlled. Residue levels of terbacil and its metabolites in alfalfa forage from plots treated postharvest with 1.1 kg/ha of terbacil were well below the 5-ppm tolerance level established for terbacil in alfalfa. There was some chlorosis of alfalfa foliage following the postharvest use of the 1.1 kg ai/ha of terbacil. There was also a yield reduction of alfalfa in two of the six experiments (one from a March and one from a June application) where 1.1 kg ai/ha of terbacil was evaluated.