Greenhouse and laboratory studies were conducted to determine the degree of dominance of the monogenic sulfonylurea herbicide resistance trait in diploid sugarbeet by comparing the response of homozygous and heterozygous resistant sugarbeet to primisulfuron, thifensulfuron, and chlorimuron on the whole plant and acetolactate synthase (ALS) enzyme level. Progeny tests suggested that the monogenic sulfonylurea herbicide resistance was semidominant. Subsequently, heterozygous resistant (R-1) and homozygous resistant (R-2) sugarbeet lines were sprayed with increasing rates of primisulfuron, thifensulfuron, and chlorimuron, and herbicide rates required for 50% growth reduction (GR50) were determined. GR50 values were also determined for homozygous susceptible sugarbeet lines (S-1 and S-2). The GR50 values indicated that the R-2 sugarbeet was 377, 269, and 144 times more resistant to primisulfuron, thifensulfuron, and chlorimuron, respectively, than susceptible S-2 sugarbeet. In contrast, R-1 sugarbeet was only 107, 76, and 57 times more resistant to primisulfuron, thifensulfuron, and chlorimuron, respectively, than S-1 sugarbeet, indicating at least a twofold difference in the magnitude of resistance between homozygous resistant and heterozygous resistant sugarbeet lines. ALS enzyme activity analysis were consistent with whole plant results. Thus, based on these two, maximum crop resistance can be obtained by developing homozygous resistant cultivars.

Field trials indicated that addition of ammonium sulfate to imazethapyr plus nonionic surfactant increased quackgrass control, especially at low imazethapyr rates. In greenhouse experiments, approximately twice as much imazethapyr was absorbed by quackgrass leaves when the herbicide was applied in combination with nonionic surfactant plus ammonium sulfate than when the herbicide was applied with nonionic surfactant alone. Black Mexican Sweet maize (BMS) suspension-cultured cells were used to evaluate the effects of ammonium sulfate and nonionic surfactant on cellular absorption of imazethapyr in the absence of a cuticular barrier. Imazethapyr absorption by BMS cells was diffusion-mediated, energy-dependent, and exhibited a pH optimum of approximately 3. Over the concentration range of 0.1 to 10.0 μM, the equilibrium concentration of imazethapyr in BMS cells was a linear function of the external concentration. Addition of ammonium sulfate to the external medium of BMS cells enhanced both the rate of imazethapyr uptake and medium acidification. There was a linear correlation between the ability of ammonium sulfate (0.5 to 10 mM) to promote medium acidification and imazethapyr uptake by BMS cells. The ammonium sulfate-induced stimulation of imazethapyr absorption in BMS cells was sensitive to plasma membrane adenosine triphosphatase inhibitors (sodium vanadate, diethylstilbestrol), the uncoupler carbonyl cyanide m-chlorophenylhydrazone, and energy metabolism inhibitors (sodium azide, nitrogen gas), demonstrating that this effect was dependent on ATP production and the functioning of the plasma membrane ATPase. It is hypothesized that cytoplasmic acidification in BMS cells due to ammonium assimilation stimulates the plasma membrane ATPase to pump protons across the plasma membrane which in turn acidifies the cell wall promoting cellular accumulation of imazethapyr by ion-trapping. Cell wall acidification due to ammonium assimilation may contribute to the ability of ammonium sulfate to enhance the efficacy of imazethapyr and other foliar-applied herbicides.

Investigations of smooth crabgrass growth and fenoxaprop-ethyl retention, foliar penetration, translocation, and metabolism were conducted at various soil moisture levels using a polyethylene glycol (PEG) semipermeable membrane system. The activity of fenoxapropethyl was significantly reduced at higher levels of moisture stress and this antagonistic effect was greater with increased duration of water deficit following herbicide application. Fenoxaprop-ethyl spray retention decreased linearly (23% total reduction) as soil matric potential (Ψm) decreased from −0.01 to −0.1 MPa. Foliar penetration and translocation of 14C-fenoxaprop-ethyl applied on the third true leaf were not affected by level or duration of moisture stress. Only 2% of the absorbed radioactivity was translocated out of the treated leaf for each moisture stress level and duration. As the soil Ψm decreased (−0.01 to −1.0 MPa) the relative levels of fenoxaprop-ethyl increased by 76 and 65% after a 48- and 96-h postapplication moisture stress period, respectively. In contrast, fenoxaprop acid decreased by 59 and 44% after 48 and 96 h of moisture stress, respectively. The relative level of fenoxaprop acid was linearly correlated to the antagonistic effect on shoot dry weight. These results suggest that decreased spray retention and, particularly, alterations in fenoxaprop-ethyl metabolism contribute to reduced fenoxaprop-ethyl activity observed in moisture-stressed smooth crabgrass.

The translocation pattern and distribution in rhizomes of 14C-glyphosate, 14C-quizalofop, and 14C-sucrose was examined in five quackgrass biotypes. Translocation of radioactivity in the different plant parts varied among biotypes. Translocation in the whole plant after treatment with the three 14C-chemicals varied among biotypes but was not correlated with their tolerance to herbicides. Detailed analysis of distribution of radioactivity in the primary rhizome showed that more 14C was found in the apical sections after treatment with 14C-sucrose. A similar pattern was observed after 14C-glyphosate application with all biotypes except one. Very low radioactivity was found in rhizomes after 14C-quizalofop application, but a preferential accumulation in apical sections of the primary rhizome was detected in two biotypes. The tolerance of one biotype to glyphosate was explained by the absence of radioactivity accumulation in the apical sections of the rhizome.

Mixtures of chlorimuron and 2,4-DB were additive with respect to crop injury and were either additive or slightly antagonistic with respect to weed control in greenhouse experiments. Absorption and translocation of 14C following application of 14C-chlorimuron and 14C-2,4-DB were not affected by the presence of the other unlabeled herbicide, except in Florida beggarweed and peanut where 2,4-DB affected distribution of 14C-chlorimuron in the treated leaf. In field studies, maximum efficacy was obtained with mixtures of chlorimuron plus 2,4-DB applied 7 or 9 wk after planting. Florida beggarweed control was greatest with chlorimuron or chlorimuron mixtures while the addition of 2,4-DB to chlorimuron improved morningglory and sicklepod control. At 9 and 11 wk after planting, addition of 2,4-DB to chlorimuron controlled Florida beggarweed better than chlorimuron alone. Peanut yields were increased by the addition of 2,4-DB at later applications.

Greenhouse and field studies were conducted to compare root development of jointed goatgrass to winter wheat. Time of seminal root development in jointed goatgrass was similar to that of winter wheat and to root development predicted by a model. The only exception was the epiblast node roots (−1A and −1B), which developed approximately 1 phyllochron later in jointed goatgrass than in winter wheat. At crown nodes, A and B roots developed later in jointed goatgrass than in winter wheat, but development of X and Y roots at these nodes was similar for both species. First-order branching at a given root axis in jointed goatgrass roots began approximately 1 phyllochron after development of that axis, compared to 2.5 phyllochrons for winter wheat and the model. Second- and third-order branching of jointed goatgrass roots began 1 and 0.5 phyllochrons earlier than the respective branching of winter wheat roots. Leaf and tiller development followed the same pattern with time for both species. Maximum distance between crown roots was 1.5 to 3 times greater in winter wheat than in jointed goatgrass. Root length density at the 0- to 10-cm depth was less for jointed goatgrass than for winter wheat, but there were no differences at greater depths. The frequency of branching of first-order laterals was greater for jointed goatgrass than for winter wheat. In the field, the relationship of jointed goatgrass shoot development to accumulated growing degree-days was linear (R2 = 0.97), with a slope and y-intercept similar to winter wheat (R2 = 0.95). These data, showing slight differences in root growth and development between jointed goatgrass and winter wheat, may be used in predictive modeling to better understand the biology and ecology of each species, and may be used in conjunction with other models to establish weed threshold levels and improve selective placement of fertilizers and herbicides to benefit crop development.

To understand persistence strategies of meadow and western salsify, ecophysiological characteristics of their seed germination were studied. Anaerobiosis (immersion in deoxygenated water) induced secondary dormancy in seeds of both species. Dormancy could be induced in 86% of meadow salsify seeds and in 65% of western salsify seeds by a 1-d anaerobiosis treatment. The induced dormancy was gradually released during storage of air-dried secondary dormant meadow salsify seeds, and the rate of this release was influenced by storage temperature; 30 C was more effective than 10 or 20 C in releasing secondary dormancy. These results suggest that the two species may rely on induced dormancy as an option in their persistence strategy. The optimum temperature for germination of nondormant seeds of both species was 15 C. Maximum germination percentages for both species were established within 4 to 6 d of incubation at 15 C and within 14 to 28 d of incubation at 25 C. Nondormant seeds did not germinate below 10 or above 30 C. Stratification (at 5 C for 2 to 10 wk) stimulated germination of secondary dormant seeds of meadow salsify. This stratification requirement can be important in preventing germination of dormant salsify seeds in the fall, thereby avoiding high seedling mortality in the winter. Light (red and far-red) had no effect on germination of seeds in secondary dormancy. In a separate study, seeds of both species were planted in pots at depths of 2 to 14 cm and seedling emergence was observed. Maximum emergence occurred when seeds were buried 2 cm deep. Seeds planted 8 cm or deeper germinated but did not emerge.

In a replacement series study, barley was more competitive than green foxtail and wild oat at 28/22 and 22/16 C. Wild oat was more competitive with green foxtail at 22/16 C than at 28/22 C. Maximum green foxtail dry weight and leaf area was produced at 28/22 C. As temperature increased, maximum dry weight and leaf area occurred earlier during plant growth. Similarly, maximum wild oat leaf area occurred earlier with increasing temperature, but the greatest leaf area was observed at 16/10 C. Leaf area ratio (LAR) and relative growth rate (RGR) of wild oat did not differ markedly between temperature regimes. Green foxtail LAR was higher while RGR was lower at 16/10 C than at 22/16 or 28/22 C. Under the 16/10 C regime green foxtail produced little mature seed. Greatest seed numbers were produced at 28/22 C. Wild oat produced mature seed under all temperature regimes with the greatest seed numbers produced at 22/16 C.

Itchgrass biotypes from 34 countries or territories were evaluated under controlled and natural day length at Houma, LA. They could be placed in five broad groups based primarily on the effect of day length on flowering but also on general morphology and pattern of growth. The Group 1 biotypes (Argentina, Bolivia, Costa Rica, Cuba-1, Ecuador-1, Florida, Guadeloupe, Honduras-1, Louisiana-1, North Carolina, Papua New Guinea-1, Panama, Peru, Philippines-1, and Venezuela) were not affected by day lengths of 12 and 14 h (day length neutral) and flowered 35 to 57 d after germination. The Group 2 biotypes (Australia, Kenya-1, Reunion, South Africa, and Zimbabwe) also were not greatly affected by photoperiod and flowered 54 to 67 d after germination, and were taller with fewer and larger diameter culms than the Group 1 biotypes. The Group 3 biotypes (Brazil, Colombia, Jamaica, and Philippines-2) were moderately sensitive to photoperiod. They took longer to flower at 14 h than at 12 h and flowered in 63 to 95 d. The Group 4 biotypes (Cuba-2, Dominican Republic, Ecuador-2, Honduras-2, India, Louisiana-2, Malaysia, Papua New Guinea-2, Puerto Rico, Taiwan, and Thailand) were short-day plants that did not flower in the screenhouse until August or September (130 to 163 d), after the natural photoperiod had decreased to 13 h. The Group 5 biotypes (Indonesia, Kenya-2, Nigeria, Sudan, Tanzania, and Trinidad) were strict short-day plants that flowered only in September to November (167 to 226 d), after the day length had decreased to about 12 h. Most biotypes were diploids with 2n = 20 chromosomes, but some were polyploids (2n = 40 or 2n = 60). The shape of the glume tip on the pedicellate spikelet was found to be acuminate, acute, or obtuse for the biotypes with 2n = 20, 40, and 60 chromosomes, respectively, and was a reliable marker to distinguish diploids from polyploids.

Jointed goatgrass is a serious weed in winter wheat, and presently no herbicides are available for its selective control. This study examined the effect of time of emergence and removal on jointed goatgrass interference in winter wheat, as well as its rate of development and soil water extraction. The goal of this study was to suggest cultural practices that minimize jointed goatgrass interference in winter wheat. Jointed goatgrass development was identical to ‘Vona’ winter wheat in two crop seasons, even though precipitation differed drastically between seasons. Depth of soil water extraction of both species was also similar. Jointed goatgrass at 18 plants m−2 reduced grain yield 27 and 17% when emerging 0 and 42 d after Vona, respectively. The relationship between time of jointed goatgrass emergence after winter wheat and grain yield loss was Y = 30.6–0.29X (X = days, r = 0.72), indicating that plants emerging in late fall still caused yield loss. Removing jointed goatgrass by early March prevented winter wheat grain yield loss. The interference data suggests that producers assess infestation levels and plan control measures in early March.

Destructive growth analysis of field replacement series experiments with mayweed chamomile and dry field peas was used to determine the competitive relationship between the two species. Mayweed chamomile produced similar amounts of leaf area and dry weight per plant in a dry year and a wet year. On the other hand, dry field peas produced 20% more leaf area and 100% more dry weight per plant in the wet year compared to the dry year. Height, leaf area, and dry weight of peas reached maximum between bloom and pod-set, and then declined. Height and dry weight of mayweed chamomile increased steadily throughout the growing season. Mayweed chamomile leaf area reached a maximum at the beginning of flower stem elongation. Initially, the relative growth rate of mayweed chamomile was about three times greater than the rate for peas, but by 40 to 48 d after planting, rates were similar for both species. Relative yields and relative crowding coefficients for dry weight showed that peas were 3 to 20 times more aggressive than mayweed chamomile. Results of these experiments show that mayweed chamomile is a weak competitor against peas, but because it continues to grow after peas senesce, it could interfere with crop harvest.

Field experiments were conducted in 1990 and 1991 at Lethbridge, Alberta, to determine if narrower row spacings and increased plant densities than commonly practiced would improve safflower's competitive ability with weeds. A factorial set of treatments of safflower at two row spacings of 11 and 22 cm and six densities ranging from 10 to 192 plants m−2 grown weed free or infested with green foxtail (Setaria viridis (L.) Beauv.). Decreasing safflower row spacing from 22 to 11 cm slightly improved competition with green foxtail, but increasing safflower density had a much greater effect. Weed-free safflower biomass and seed yield plateaued at 70 and 84 plants m−2 in 1990 and 1991, respectively. However, safflower infested with 500 plants m−2 of green foxtail increased in biomass and seed production up to 100 plants m−2 in 1990 and 156 plants m−2 in 1991. At these high densities, weedy safflower yielded less than weed-free safflower but three to four times more than achieved at lower stand densities. Concurrently, high safflower density reduced green foxtail biomass up to 72% and seed yield up to 85%. Dense safflower stands developed a closed canopy early in the season and shaded green foxtail more than thin stands.

Adverse changes in weed communities are a limiting factor for the adoption of conservation tillage practices. Predictions of an increased association of annual and perennial grasses, perennial dicot weeds, wind-disseminated species, and volunteer crops as weeds, and decreased association of annual dicot weeds in reduced-tillage systems were tested. Field experiments involving zero-, minimum-, and conventional-tillage systems were conducted in Saskatchewan from 1986 to 1990 at Ituna and Waldron, and from 1986 to 1988 at Tadmore. Weed community composition was analyzed for years 1988 to 1990 by canonical discriminant analysis. An increased association of perennial and annual grasses with zero tillage did not generally occur. Wind-dispersed species and volunteer crops were associated with reduced tillage and summer annual dicots with conventional tillage, but exceptions occurred. Species responded differently among sites or within a site over time. Within the time frame of this research, changes in weed communities were influenced more by location and year than by tillage systems, indicating fluctuational rather than directional or consistent changes in community composition.

Field studies determined flowering pattern, seed production, and somatic polymorphism of pitted morningglory, common cocklebur, and sicklepod under interspecific and no interference with soybean. Flowering patterns differed between years but were similar under both interferences. Interspecific interference reduced flower and seed numbers 85 to 99%. Rainfall had little effect on flowering, but photoperiod and temperature responses were evident for all weed species. The production equations for seed number per plant from dry weight were quadratic for pitted morningglory and common cocklebur but linear for sicklepod. All species showed somatic polymorphism; however, differences in seed germination and viability were not consistent between years.

In experiments conducted under controlled conditions, glyphosate-induced inhibition of rhizome bud growth in quackgrass was reduced by increasing the nitrogen (N) concentration in the nutrient solution from 10.5 to 210 mg L−1 either 2 or 4 d before foliar application of the herbicide, and for 7 d after the herbicide treatment. The additional N reduced the glyphosate-induced inhibition of rhizome growth on the intact plant at the lowest glyphosate dosage (2.8 μg per plant) but had no significant effect at higher dosages. Both the 2- and 4-d high N treatments significantly increased the length, dry weight, and water content of the lateral buds by the time the herbicide was applied. They also increased bud growth on the glyphosate-treated plants during the 7 d following the herbicide application and promoted release of the buds from inhibition during a subsequent bioassay of their regenerative growth on excised, single-node rhizome segments. These effects of N supply interacted significantly with glyphosate dosage, bud position on the rhizome, and duration of incubation. It is postulated that bud growth response to increased N supply may reduce the inhibiting effect of the herbicide by diluting the glyphosate concentration in the bud, or that the additional N may counteract the toxic action of glyphosate on amino acid metabolism or protein synthesis.

This field research was designed to compare the long-term effectiveness of late-September applications of several herbicides for reducing Canada thistle shoot density on noncropped, untilled abandoned farmland when reapplied annually for 3 yr. Clopyralid at 560 and 840 g ae ha−1 or picloram at 280 and 560 g ae ha−1 reduced Canada thistle shoot density as well as either glyphosate3 at 0.8 to 2.8 kg ae ha−1 or dicamba at 1.1 and 2.2 kg ae ha−1. These treatments were much more effective than 2,4-D at 1.1 and 2.2 kg ae ha−1, chlorsulfuron at 34 and 67 g ai ha−1, and metsulfuron at 34 and 67 g ha−1 for progressively reducing Canada thistle shoot density over three annual fall applications. Picloram and clopyralid greatly reduced and delayed shoot emergence from adventitious root buds in spring after two fall-applied treatments compared with nontreated checks.

Reduced-tillage practices have allowed leafy spurge to infest cropland. The reduction in leafy spurge infestation and winterhardiness by fall tillage, N application, or herbicide treatment was evaluated. Cultivating leafy spurge twice each fall for 3 yr provided complete control. Cultivation followed by N application at 225 kg ha−1 reduced the stand by 85% after 3 yr, whereas N applied alone had little effect. Picloram plus 2,4-D at 0.6 plus 1 kg ha−1 reduced the infestation to 65% of the untreated control after 2 yr but then the infestation declined rapidly to 1% after a third application. Leafy spurge cold tolerance decreased as root depth increased. The GR50 and LT50, the temperatures required to reduce total dry weight and survival, respectively, by 50% varied by root depth and treatment. For example, the LT50 and GR50 for untreated leafy spurge averaged −19 and < −20 C for crowns, respectively, compared to the −16 and −18 C for roots from 0 to 15 cm deep and −13 and −12.5 C for roots from 15 to 30 cm deep. The GR50 and LT50 temperature after 2 yr of treatment was reduced to 0 C by two cultivations in fall or picloram plus 2,4-D. Carbohydrate content was not a good indicator of winterhardiness. Cultivation reduced the leafy spurge root system faster than herbicide treatment especially at the 15- to 30-cm depth.

Common cocklebur is a very competitive and difficult to control weed species in soybean production. Field research was conducted at Rosemount, MN, from 1989 to 1991 to evaluate reduced rates of bentazon applied broadcast or banded over the crop row in combination with interrow cultivation for common cocklebur control in soybean. Bentazon at 0.6 kg ai ha−1 applied in a 38-cm-wide band over the soybean row followed by two cultivations controlled almost 90% of the common cocklebur when environmental conditions were favorable, and the majority of the common cocklebur emerged prior to bentazon application. However, when precipitation was below normal prior to bentazon application and a high proportion of the common cocklebur emerged after the initial bentazon application, sequential bentazon treatments controlled more common cocklebur and resulted in greater soybean yields than combinations of bentazon plus cultivation. Differences in soybean yield were attributed to differences in common cocklebur control rather than injury from cultivation or bentazon.

Laboratory experiments were conducted to determine the effect of three phenolic acids, acetone, and riboflavin on aqueous photolysis of chlorimuron and imazaquin. The phenolic acids investigated were caffeic acid (CA), ferulic acid (FA), and p-coumaric acid (PCA). Treatment solutions were contained in quartz vessels and irradiated with 300 to 400 nm UV light in a photoreactor. The extrapolated photolysis half-life of chlorimuron in pure solution was 107 h, compared to a half-life of 0.42 h for pure aqueous imazaquin. Chlorimuron in solutions containing 10 ppmw riboflavin, acetone, CA, FA, or PCA exhibited half-lives of 9, 57, 58, 67, and 146 h, respectively. Imazaquin in solutions containing 10 ppmw riboflavin, acetone, CA, FA, or PCA had half-lives of 0.70, 0.55, 0.55, 0.48, and 0.55 h, respectively. The presence of PCA in aqueous media delayed chlorimuron photolysis, whereas all other compounds, especially riboflavin, sensitized chlorimuron photolysis. In contrast, imazaquin photolysis was delayed in the presence of the test compounds, with riboflavin having the greatest effect and causing in a 68% increase in imazaquin half-life over that of imazaquin alone. Quantum yields for sensitized photolysis of chlorimuron by riboflavin and for riboflavin by imazaquin were 0.1134 and 0.0477, respectively. These results suggest that some soluble and naturally occurring organic compounds may enhance chlorimuron photolysis yet delay imazaquin photolysis in surface waters.

Comparative spread of droplets of several different oils and water with different adjuvants on leaf surfaces was investigated. Spread was better on the lower surface of johnsongrass leaves than on upper leaf surfaces with nine of 14 oils studied; two spread best on upper leaf surfaces, and three were equal in spread on both surfaces. Differences in spread coefficients did not appear to be directly related to surface tension, viscosity, or mid-boiling point of the oils. Soybean or cottonseed oils did not spread as well as petroleum oils but methylated soybean and sunflower oils had high spread coefficients on both upper and lower leaf surfaces. All but one petroleum-base oil spread three to four times better on leaf surfaces than on oil-sensitive paper. Water mixtures of an organosilicone surfactant spread much better on water-sensitive paper and on johnsongrass leaves than water with conventional adjuvants, but spread of paraffinic oils exceeded that of any water-adjuvant mixture. Spread coefficients of most petroleum-base oils were better on lower than upper leaf surfaces. Spread usually increased as the age of leaves increased from 14 to 56 d. Water droplets with adjuvant had at least an 86% weight loss after 6 min, but low volatile paraffinic oil droplets had little weight loss 2 d after application.