Vegetation and climate over approximately the past 13,000 yr are reconstructed from fossil pollen in a 9.4-m mire section at Caleta Róbalo on Beagle Channel, Isla Navarino (54°56′S, 67°38′W), southern Tierra del Fuego. Fifty surface samples reflecting modern pollen dispersal serve to interpret the record. Chronologically controlled by nine radiocarbon dates, fossil pollen assemblages are: Empetrum-Gramineae-Gunnera-Tubuliflorae (zone 3b, 13,000–11,850 yr B.P.), Gramineae-Empetrum-assorted minor taxa (zone 3a, 11,850-10,000 yr B.P.), Nothofagus-Gramineae-Tubuliflorae-Polypodiaceae (zone 2, 10,000–5000 yr B.P.), Nothofagus-Empetrum (zone 1b, 5000-3000 yr B.P.), and Empetrum-Nothofagus (zone 1a, 3000-0 yr B.P.). Assemblages show tundra under a cold, dry climate (zone 3), followed by open woodland (zone 2), as conditions became warmer and less dry, and later, with greater humidity and lower temperatures, by closed forest and the spread of mires (zone 1). Comparisons drawn with records from Antarctica, New Zealand, Tasmania, and the subantarctic islands demonstrate broadly uniform conditions in the circumpolar Southern Hemisphere. The influences of continental and maritime antarctic air masses were apparently considerable in Tierra del Fuego during cold late-glacial time, whereas Holocene climate was largely regulated by interplay between maritime polar and maritime tropical air.