The 1981 Autumn Symposium (held at the Zoological Society, London, on 31 October) was a new departure for the British Society for Parasitology in that Cambridge University Press have taken over publication of the proceedings. The style and layout of the contributions have been changed in order to conform with the format currently used in the journal Parasitology. The symposia proceedings will now be published as separate issues of Parasitology but with their own distinctive colour and cover design. The proceedings will be available by subscription to the journal or separately. Furthermore, the meeting organizers will in future be responsible for editing the symposia: assistance will be by Drs Taylor and Muller during a transition period.

In three separate experiments the behaviour of Dermacentor andersoni larvae on normal and tick-resistant guinea-pigs was studied at intervals throughout 5-day infestation periods. Fewer larvae survived on resistant hosts and their mean sizes were less than those of larvae on normal hosts, the differences becoming more obvious as time progressed. When measured at 32°C, the peristaltic rates of midgut diverticula of the larvae surviving on resistant hosts were consistently lower than those of larvae from normal hosts. Mean surface temperatures of infested ears were higher on resistant than on normal hosts. This was assumed to be a result of cutaneous hypersensitivity reactions in the resistant animals. Larvae detached, or changed attachment sites, more often on resistant than on normal hosts. This was consistently evident after 26 h post-infestation and correlated with the known timing of basophil cell infiltration and degranulation in the skin of infested, resistant guinea pigs. It appeared that most larvae, after detaching from the resistant host, died off the host. However, some died in situ while still attached. Such deaths occurred on resistant but not on normal animals between 8 and 26 h post-infestation.

The relationship between intensity and site of infection of Diorchis brevis, D. inflata and D. ransomi in Fulica atra from central Poland has been analysed; of 173 adult birds 152 (87·9%) were infected with one or more of these three species. From (1) the incidence of the three species (D. brevis, 59·5%; D. inflata, 69·9%; D. ransomi, 61·3%), (2) the high total incidence and high total intensity of infection (118·2 worms/bird) in hosts harbouring the three species concurrently and (3) the frequent co-occurrence of all three species in the same site in the host gut, it is concluded that there are no antagonistic interactions among these species. There are, however, some inter-dependencies between the number of parasites and the site occupied by particular species. In heavy infections there was a tendency to specific segregation and extension of the site of each species. This suggests the existence of inter- and intra-specific competition at higher densities of infection, regulating the spatial distribution of these parasites in the host gut.

Plasmodium falciparum was grown in vitro in blood taken from naturally infected Gambian patients, and the development of the cultured sexual parasites was studied by light and electron microscopy. The young (Stage II and III) female gametocytes undergo a single cryptomitotic nuclear division. This division immediately follows the S phase which Sinden & Smalley (1979) have demonstrated in the Stage I and II gametocytes of both sexes. The male gametocytes, by contrast, do not undergo mitosis during their maturation period in the erythrocyte and thus remain polyploid. Hence the cell cycles of the male and female gametocytes differ significantly. The ultrastructural basis of the characteristic changes in shape of the developing gametocyte are shown to be due to the assembly and subsequent loss of components of the sub-pellicular membranous and microtubular cytoskeleton. Stage I–III gametocytes synthesize numerous ribosomes and endoplasmic reticulum. This correlates with the active synthesis of RNA and protein in these young parasites (Sinden & Smalley, 1979). The marked reduction in macromolecular synthesis in the mature parasites is paralleled by a reduction in cytoplasmic ribosome density in the male gametocyte only. In the female, however, this reduction in activity is correlated with the appearance of a nucleolus. These changes suggest that different mechanisms are being used to control RNA synthesis in the two sexes of gametocyte.

The population dynamics of insect–pathogen interactions are examined with the aid of simple mathematical models. Three concepts of central importance to the interpretation of population behaviour are discussed, namely the ability of the pathogen to persist within its host population, the ability to regulate and depress host population abundance, and the ability to induce non-seasonal cyclic changes in host density. The selection of pathogen species or strains to depress pest population growth is discussed and the optimal characteristics are shown to be intermediate pathogencity combined with an ability to reduce infected host reproduction, high transmission efficiency, including elements of vertical as well as horizontal transmission stages. When the pathogen plays a significant role in the regulation of host population growth, it is argued that many insect–pathogen interactions will exhibit non-seasonal oscilations in host and pathogen abundance. Mathematical models are used to explore the patterns of population behaviour that result from the continual introduction of a pathogen into a target pest population. It is shown that there exists a critical introdution rate, above which the eradication of the pest is theoretically possible. Significant reductions in pest population abundance will not occur until the introduction rate approaches this critical value, whereupon the oscillatory behaviour of the interaction between host and pathogen population will be suppressed.

A general dicussion is given of the problems arising from the combined use of chemical agents and pathogens for the control of pest species, and the evolutionary pressures acting on host and pathogen populations.

The effects of Eimeria maxima or restricted pair-feeding on weight gain, plasma concentrations of protein, glucose, free fatty acids (FFA) and uric acid and liver glycogen were compared in immature fowl. Food intake/kg body weight and weight gain decreased during the acute phase of infection (days 5–7) while weight loss was prolonged for an extra day compared with pair-fed birds. During recovery, food intake/kg body weight of infected birds was greater than that of non-infected controls but there was no evidence for an increase in growth rate compared with controls when body weight was considered. Growth rate of pair-fed birds was greater than that of infected birds during recovery, indicating their better use of ingested food. Liver glycogen and plasma protein concentration were decreased during the acute phase of infection but the concentrations of plasma glucose, free fatty acid (FFA) and uric acid were not affected. In pair-fed birds liver glycogen was depleted, concentrations of plasma glucose and uric acid decreased and FFA increased, and these changes persisted for the remainder of the experiment. The findings are similar to those in birds whose food has been withheld and were probably due to the pattern of food intake imposed by the experimental protocol. It is concluded that the metabolic differences between infected and pair-fed birds are of doubtful significance.

The circadian migration of Hymenolepis diminuta in the small intestine of the rat may be correlated with a circadian variation in 5-hydroxytryptamine levels present in worm tissue, in the intestinal lumen, in the intestinal mucosa, with the amount of food present in the small intestine and in arterial blood. The 5-HT and food levels in uninfected animals were also determined. The 16.00 h stage in the circadian cycle marks both the commencement of host feeding, followed by rising 5-HT levels in both worm and host tissues, and initiation of an anteriad migration of worm biomass. It was found that 5-HT levels in the intestine of parasitized animals were significantly higher than in the intestine of uninfected controls. This is the first report of circadian variation in mucosal and luminal 5-HT levels. The similarity in the circadian patterns of worm migration and worm luminal, mucosal and blood 5-HT levels were striking. Fasting eliminated the circadian rise in intestinal 5-HT levels and the worms did not migrate. Luminal 5-HT levels were significantly lower in fasted animals than in the comparable rats fed ad libitum. When the intestine was ligatured at the pyloric sphincter, worm anteriad migration still occurred after feeding, indicating that the presence of exogenous food in the intestine is not a factor in the initial migration of the worms.

An electron microscopical study was made on the development of Eimeria dispersa in the small intestine of the domestic turkey. Turkey poults, 10–14 days of age, were inoculated with oocysts and pieces of intestinal tissue were fixed at intervals between 3·5 and 114 h after inoculation. Sporozoites were occasionally seen in enterocytes but more often in ‘pale’ cells closely resembling lymphocytes. These cells were insinuated between enterocytes and in this study are referred to as intestinal intra-epithelial leucocytes (IEL). Maturation of the first-generation of schizogony occurred in this type of cell. Other generations of schizonts and gametogony occurred in epithelial cells and were characterized by the presence of ‘spines’ arranged around the parasitophorous vacuole extending into the cytoplasm of the host cell. A limited study of the parasite in the Bobwhite quail, Colinis virginianus, showed that development occurred in similar cells to those of the turkey. The ‘spines’, which were characteristic of later stages of E. dispersa in the turkey, were also present in this host and seemed to be a characteristic of E. dispersa, not of the host species.

The Houghton (H) strain of Eimeria acervulina was attenuated by serial passage through chickens of the first oocysts produced during infection. This selection pressure resulted in a reduction in the pre-patent period of the parasite, shown to be due to the selection of a line predominantly with only 3 instead of 4 generations of schizonts. The precocious line had a reproductive potential much lower than that of the parent strain and it was significantly less pathogenic. Chickens given oocysts of the precocious line were almost completely immune to challenge with the Houghton strain.

Virus diseases have been reported from more than 800 species of insects and mites. Isolates of the baculovirus and cytoplasmic polyhedrosis virus groups have biological properties which should lead to their successful use as microbial control agents in integrated pest management programmes. These viruses infect the larval stages of many lepidopterous and hymenopterous pests, producing a chronic or lethal infection and the release of large quantities of relatively stable infective inclusion bodies (IBs). The IBs serve as the means by which the viruses are transmitted and persist outside the host. Younger larvae are more susceptible to infection than older stages, and this difference influences the timing of application and doses of virus needed for practical pest control. The high degree of host specificity of many isolates reduces their potential ecological hazard but also limits their use, particularly on crops where a complex of pests is established. Environmental persistence is also a limiting factor as virus is rapidly inactivated by ultra-violet light even when contained within IBs. The viruses persist for longer periods when transmitted within the host population, a feature of virus infections restricted to the insect gut.

The practical use of insect viruses in horticulture and agriculture does not utilize their full epizootic potential, but takes advantage of their high pathogenicity and specificity. The baculoviruses of codling moth, and Heliothis spp. provide satisfactory pest control, but for their most cost-effective use it is important to determine the minimum dosage rates of virus required. It is encouraging that studies of the virus control of Pieris spp. have suggested that control achieved by the insecticidal use of a virus can be closely predicted from information on dosage-mortality responses, larval feeding rates and virus persistence. The stability of forest and grassland, and their high economic thresholds makes them ideal candidates for longer-term control. Viruses of the coconut rhinoceros beetle and european spruce sawfly provide examples of classical biological control where the viruses persist for long periods, are efficiently transmitted and act as natural regulators of their hosts. Virus control of pasture, and some forest, pests may be possible by manipulating enzootic viruses without the need for direct control measures. More frequently insecticidal applications are needed, providing control of limited duration which requires periodic ‘topping-up’.

Few viruses are commercially-available; their selectivity and often small potential market, may limit industrial interest. However, improvements in virus production, formulation and a better understanding of virus epizootiology should lead to an increasing role for this group of insect pathogens in biological control.

A prediction of the hypothesis of Wilson (1977, 1980a, b) to account for larval migration of homogonic Strongyloides ratti in the host is that the pattern of invasion of the mammary gland of a lactating rat will be quantitatively similar on both sides and independent of the point of entry into the body. Twenty-one suckled mother rats in 6 experiments in which live 75Se-labelled 3rd-stage homogonic larvae were injected under the skin of the upper flank had an overall distribution of label 30 h post-injection, as a percentage of the initial dose, in the quadrants, I (rear, injection side), II (rear, opposite injection side), III (front, injection side) and IV (front, opposite injection side) of the mammary gland as follows: 27·4%, 1·27%, 1·89% and 1·24%. Quantitative changes in mammary label between 30 and 48 h post-injection using live larvae, differences between mothers and virgins, and results after injection of heat-killed labelled larvae, confirm that the pattern is representative of the behaviour of normal (unlabelled) worms when injected. The theory is therefore disproved. The findings are put forward as the first quantitative evidence for major lymphatic involvement in migration of a skin-penetrating round worm. They need confirmation in similar experiments in which worms are allowed to penetrate the skin naturally. The role of isotope-labelled larvae versus traditional methods of estimating parasite content of host tissue is discussed.

An egg-adapted line of Eimeria necatrix has now been passaged 40 times in the chorio-allantoic membranes of embryonated eggs. Between the 21st and 40th passage in eggs the parasite was subjected to a selection for precocious development, and its pre-patent period in this host is now 123–125 h. The parasite continued to adapt to the chorio-allantoie this tissue caused the growth of the host embryo to be markedly stunted. In chickens, both the reproduction and the pathogenicity of the egg-adapted line decreased with increasing numbers of passages in eggs. A comparison of teh immunogenicity of te egg-adapted line and its parent strain in chickens kept on litter, showed that substantially more oocysts of the egg-adapted line had to be given to induce complete protection.

Eimeria funduli, a coccidium infecting the liver and pancreas of killifishes and requiring an intermediate host, was allowed to develop at 22±2 °C for 5, 10, 15 or 20 days (schizont through zygote stages) in Fundulus grandis and was then exposed to 10·0±0·5 °C for 20 days or to 7·0±0·5 °C for 5 or 20 days. Both low temperature treatments inhibited all developmental stages, except for the case of fish infected for 5 days before exposure to 7 °C in which infections were eliminated. Returning fish back to a temperature of 22 °C for 20 days resulted in resumed development of all stages at the same or somewhat reduced rate. In all cases, intensity of infections was greatly reduced during exposure to low temperatures. Many organisms exhibited abnormalities. Gamonts and zygotes were atrophied or disintegrated within their parasitophorous vacuoles in all exposures and, in fish exposed to 7 °C for 20 but not 5 days, about 30% of the oocysts were abnormal, with from 1–8 sporoblasts. Areas of degeneration or necrosis incorporated aggregates and single parasites in both the pancreas and the hepatic parenchyma. The granulocytic inflammatory response, which at 22 °C was normally restricted to the period spanning development of gamonts and formation of the oocyst wall, was inhibited or delayed from infiltrating infected, diseased areas at low temperatures. The response intensified after cold-exposed fish were returned to 22 °C. A fibroid, non-granulocytic response occurred in fish introduced 5 days post-infection to 10°C before being returned to warm water. Two other types of lesion occurred in the pancreas. The first could develop into necrosis and was associated with low temperature; it did not always involve infected tissue. The other, a degenerating focal alteration, was not necessarily associated with infections or low temperatures.

All bacteria in microbial insecticides are species of Bacillus and form spores since they have to survive in the environment and on the shelf. They can be formulated as wettable powders, suspensions and dusts for application with conventional pest control machinery. All are safe to man and virtually all non-target organisms. Development costs are relatively low, but host specificity greatly restricts markets, the largest being ca. 2000 tons per annum in the West for B. thuringiensis. All act only after ingestion, a disadvantage because there is no contact action and usually only larvae are attacked. Three main groups have special features that determine their commercial success.

The B. popilliae group is produced only in vivo which limits production by three small firms. The Japanese beetle has been controlled in grassland in the warm parts of the USA by single applications of spores in heaps, spaced 2 m each way. The bacterium spreads slowly to untreated areas, is very persistent and kills only by infection.

The B. thuringiensis group kills larvae of Lepidoptera, mosquitoes and blackflies, mainly by gut poisoning with a protein crystal toxin. It rapidly paralyses mouthparts and gut, stopping crop damage. It is readily produced by deep liquid fermentation, but does not persist and needs repeated application during the pest season. Products containing no beta exotoxin can be applied at unlimited dosage to food crops up to harvest. Only one application is needed for stored grain. After 20 years' use of strains against Lepidoptera, a different strain is now used commercially against mosquitoes and blackflies (only 5 years after its discovery), although improvements in formulation for aquatic application are needed. A recent new product based on the beta exotoxin is used in Finland only against flies in pig houses because it has some vertebrate toxicity.

The B. sphaericus group is similar to B. thuringiensis, except that its proteinaceous toxin is different, is situated in the spore wall in strain 1593, and attacks only mosquitoes. Now at the pilot production stage, its commercial future depends on whether it is more potent than B. thuringiensis against certain species and whether it can recycle to give effective extended mosquito control in some environments.

Intensive selection from natural isolates has improved potency 100 to 600 fold. This selective effort must be maintained and improved by genetic manipulation, which can be used to develop greater potential, particularly since DNA coding for the crystal toxin is carried on plasmids. This also gives speculative hope that the toxin may be incorporated into natural aquatic bacteria for mosquito control and into plants for protection against lepidopterous larvae. A great advantage is that these bacteria do not harm beneficial fauna to cause pest resurgence. At present, the main use lies in integrated pest control systems, although bacteria are not likely to supplant chemical insecticides on a large scale in the near future.

The survival of the free-living stages of Trichostrongylus colubriformis under defined conditions of temperature and relative humidity was investigated. The survival of embryonated eggs was poor at 0, 33 and 54·5 % relative humidity (rel. hum.) at 20 °C but hatching occurred from a proportion of eggs even after exposure for 104 days to 76 and 98% rel. hum. at 20 °C. Second-stage larvae were desiccation-susceptible and were killed within 6 h even at 98% rel. hum. and 20 °C. Infective larvae, dried separately or in clumps, survived prolonged exposure to desiccation at 33–98% rel. hum. and 20 °C with 50% survival times of 58–164 days. Clump formation did not enhance survival in this range. Infective larvae also survived exposure to vacuum desiccation with 50% survival times of 8·8 h in clumps and 4·5 h when dried separately. The infective larva thus readily survives desiccation and may prove a useful model for the study of anhydrobiosis.

The cell surface properties of Plasmodium gallinaceum sporozoites have been investigated by means of microelectrophoretic and lectin-binding studies. Their electrophoretic mobility has been measured as a function of pH, the results suggesting qualitative and quantitative differences in the surface ionogenic groups between sporozoites from mature oocysts and those from salivary glands. Reaction of sporozoites with citraconic anhydride produced a small but significant increase in mobility, whereas 5,5-dithio-bis-(2-nitrobenzoic) acid had no effect on mobility; thus there appear to be amino groups but not –SH groups at the surface of sporozoites. Treatment of sporozoites with trypsin considerably reduced their mobility and suggests that a significant proportion of the cell surface charge is associated with protein. Incubation with neuraminidase, however, had no effect on sporozoite mobility and indicates that sialic acid residues, responsible for much of the negative charge associated with mammalian cells, are probably not present on the cell surface of sporozoites. Evidence for the presence of carbohydrates on the cell surface membrane of sporozoites was sought using fluorescein isothiocyanate–Concanavalin A. Results demonstrated that ligands similar to α-D-glucose and α-D-mannose are not present in an exposed or reactive form on the cell surface membrane of P. gallinaceum sporozoites.

Of the unicellular eukaryotes, formerly Protozoa, now considered to belong to five separate phyla, only the neogregarines and microsporidia are serious contenders for a role in biological control of invertebrate pests. Ciliates of the genus Lambornella, which penetrate their hosts via cuticular cysts, have potential in mosquito control but have not been investigated in depth.

‘Protozoa’ generally kill their hosts by overwhelming numbers, destroying the normal function of organs or depleting the host of essential reserves. Because they are slow-acting pathogens they cannot be used on their own when pests have already reached a high level of abundance nor can they be relied upon when the damage threshold of pests is low. Their principal use will be as the slow-acting component of a 2-pathogen or pathogen-plus-chemical formulation, which can be used when a degree of damage is tolerable.

A comparison is made between two microsporidia in lepidopteran hosts, Vairimorpha necatrix and Nosema pyrausta. The former causes high mortality in a wide range of hosts, when bacterial septicaemia ensues after disruption of the gut wall by the microsporidian invasion process. Some larvae may survive this period and live to damage crops, but none survives to adulthood. There is no transovarial transmission and the parasite is rarely found in natural populations. V. necatrix could be used as a microbial pesticide for short-term control. N. pyrausta is restricted to a single host, the European corn borer. It has low pathogenicity, causing some larval mortality especially under conditions of environmental stress. Most hosts survive to adults but show reduced longevity and fecundity. The parasite is transmitted transovarially and is highly prevalent in the field. It is not considered pathogenic enough to be used as a microbial pesticide but is an important factor in regulating natural populations. These examples illustrate the inverse relationship between pathogenicity and prevalence and show how cycles of host population abundance may be driven by pathogens of moderate to low pathogenicity.

Two kinds of transovarial transmission mechanisms are discussed. With the microsporidia of winter moth, vegetative stages and spores, even when abundant in egg yolk, do not gain access to larval tissues but are confined to the meconium in larvae just before eclosion. Larvae are not infected when they hatch but the spores are carried over in the eggs to the next period of larval feeding activity. In contrast, some genera of microsporidia in haematophagous diptera, e.g. Amblysopora in mosquitoes, actually infect the cells of developing larvae, which are already infected when they hatch.

The prospects for biological control with ‘Protozoa’ are reviewed for vectors of medical importance and for pests of pasture, field crops, forests and stored products. Particular attention is given to the use of microsporidia in combination with low concentrations of compatible chemical insecticides and with other pathogens (e.g. viruses).

Spores for field application can be produced in natural or experimental hosts by feeding or intrahaemocoelic inoculation. Yields vary according to the species of parasite and host. Examples are Nosema locustae in Melanoplus bivittatus yielding 3·9 × 109 spores/grasshopper enough to treat more than 1 hectare of rangeland, and Vairimorpha necatrix in Heliothis zea, yielding 1·67 × 109 spores per larva, with 2·5 × 1012 spores/hectare required for field application. In vitro culture is at present a laboratory tool only, with yields too low for economic returns.

Spores can be stored, according to species, dry or in distilled water with antibiotics at 4 °C. This gives good survival for months or years. In field applications feeding-bait formulations are more efficacious than sprays because they concentrate the spores for uptake by the target species and give the spores some protection from harmful ultraviolet radiation.

Pheromone lures have been used for the introduction of spores by males into pest infestations in stored grain. Males are lured to sites dusted with spores and return to the grain after removal of the lure, to contaminate females and larvae. The use of these lures, first as traps to monitor pest population increases, then to effect a controlled pest growth curve by introduction of pathogens, is an attractive innovation. Protozoa are considered safe for field application on the limited evidence available.

C57B1/6 male mice with chronic trichinellosis, and age-matched uninfected control mice, were inoculated with B16 melanoma cells. Tumour development was inhibited in the infected animals. In the infected tumour-bearing mice, the tumour induction intervals were longer and the tumour size was subsequently smaller than in the control mice. Moreover, when the number of tumour cells in the inoculum was less than that required to produce 100% tumour incidence in the uninfected mice (<1 × 104 cells), significantly more of the mice with an infection of 2 months duration remained tumour-free. These results were significant at the P<0·05 level.

Regular samples made on the 1978 cohort of brook sticklebacks (Culaea inconstans) from a swamp in Ile Perrot, Quebec, Canada were examined for Apatemon gracilis metacercariae. The prevalence of the parasite rapidly reached 100% in the fish population. The mean parasite burden increased from zero to a plateau of about 44 parasites/fish. Over-dispersion of the frequency distribution of parasites in the fish host, as measured by variance to mean ratios, increased to a peak and then decreased significantly while the mean parasite burden remained constant. The effects of parasite burden on the survival of the stickleback host are discussed, as well as the validity of the use of changes in over-dispersion for demonstrating parasite-induced mortality.

Concanavalin A added to a defined culture medium renders schistosomula more resistant to the lethal effect of antibody plus complement. The Con A dose-dependent acquisition of protection reached a plateau at a concentration of 4 μg Con A/ml. α-Methylmannoside was not able to reverse the Con A-induced protection but excess methylmannoside, added to the incubation medium together with Con A partially prevented the effect of the lectin. Binding of anti-parasite antibody to the schistosomula surface did not change after pre-incubation with Con A. In the presence of puromycin (100 μg/ml) Con A is still able to confer protection. Incorporation of labelled amino acid into the tegumental proteins was not affected by Con A. Furthermore, a progressive reduction of protection was observed as the time of culture preceding Con A addition increased.