Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-04T19:25:32.686Z Has data issue: false hasContentIssue false
  • English
  • Français

Strongyloides ratti: studies of 75Se-labelled larvae of the homogonic strain in female hosts

Published online by Cambridge University Press:  06 April 2009

P. A. G. Wilson  and
Norma E. Simpson
P. A. G. Wilson
Affiliation:
University of Edinburgh, Department of Zoology, West Mains Road, Edinburgh EH9 3JT
Norma E. Simpson
Affiliation:
University of Edinburgh, Department of Zoology, West Mains Road, Edinburgh EH9 3JT
Get access Rights & Permissions [Opens in a new window]

Summary

A prediction of the hypothesis of Wilson (1977, 1980a, b) to account for larval migration of homogonic Strongyloides ratti in the host is that the pattern of invasion of the mammary gland of a lactating rat will be quantitatively similar on both sides and independent of the point of entry into the body. Twenty-one suckled mother rats in 6 experiments in which live 75Se-labelled 3rd-stage homogonic larvae were injected under the skin of the upper flank had an overall distribution of label 30 h post-injection, as a percentage of the initial dose, in the quadrants, I (rear, injection side), II (rear, opposite injection side), III (front, injection side) and IV (front, opposite injection side) of the mammary gland as follows: 27·4%, 1·27%, 1·89% and 1·24%. Quantitative changes in mammary label between 30 and 48 h post-injection using live larvae, differences between mothers and virgins, and results after injection of heat-killed labelled larvae, confirm that the pattern is representative of the behaviour of normal (unlabelled) worms when injected. The theory is therefore disproved. The findings are put forward as the first quantitative evidence for major lymphatic involvement in migration of a skin-penetrating round worm. They need confirmation in similar experiments in which worms are allowed to penetrate the skin naturally. The role of isotope-labelled larvae versus traditional methods of estimating parasite content of host tissue is discussed.

Type
Research Article
Information
Parasitology , Volume 84 , Issue 3 , June 1982 , pp. 443 - 454
Copyright
Copyright © Cambridge University Press 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Looss, A., (1905).Wanderung der Ancylostomum- und Strongyloideslarven von der Haut nach dem Darm. Comptes Rendus de 6e Congrés internationale de Zoologie, Berne 1904, 225–33.Google Scholar
Murrell, K. D., (1980). Strongyloides ratti: Acquired resistance in the rat to preintestinal migrating larvae. Experimental Parasitology 50, 417–25.CrossRefGoogle ScholarPubMed
Tada, I., Mimori, T., & Nakai, M., (1979). Migration route of Strongyloides ratti in albino rats. Japanese Journal of Parasitology 28, 219–27.Google Scholar
Tilney, N. L., (1971). Patterns of lymphatic drainage in the adult laboratory rat. Journal of Anatomy 109, 369–83.Google ScholarPubMed
Wilson, P. A. G., (1977). The effect of the suckling stimulus on the migration of Strongyloides ratti in lactating rats. Parasitology 75, 233–39.CrossRefGoogle ScholarPubMed
Wilson, P. A. G., (1979). Tracking radioactive larvae of Strongyloides ratti in the host. Parasitology 79, 29–38.CrossRefGoogle ScholarPubMed
Wilson, P. A. G., (1980a). Migration of larval roundworms in the rat. Parasitology 81, xi.Google Scholar
Wilson, P. A. G., (1980b). Recirculation of Strongyloides larvae in the blood of rats and the use of the mammary gland as a sampling device. Proceedings of the Third European Multicolloquium of Parasitology, Cambridge, Abstract 15.Google Scholar
Wilson, P. A. G., Cameron, M., & Scott, D. S., (1978a). Strongyloides ratti in virgin female rats: studies of oestrous cycle effects and general variability. Parasitology 76, 221–27.CrossRefGoogle ScholarPubMed
Wilson, P. A. G., Cameron, M., & Scott, D. S., (1978b). Patterns of milk transmission of Strongyloides ratti. Parasitology 77, 87–96.CrossRefGoogle ScholarPubMed
Wilson, P. A. G., Gentle, M., & Scott, D. S., (1976a). Milkborne infection of rats with Strongyloides ratti and Nippostrongylus brasiliensis. Parasitology 72, 355–60.CrossRefGoogle ScholarPubMed
Wilson, P. A. G., Gentle, M., & Scott, D. S., (1976b). Dynamic determinants of the route of larval Strongyloides ratti in lactating rats and the control of experimental error in quantitative studies of milk transmission of skin penetrating roundworms. Parasitology 73, 399–406.CrossRefGoogle ScholarPubMed
Wilson, P. A. G., & Simpson, N. E., (1981). Dynamics of infection in rats given low doses of homogonic and heterogonic Strongyloides ratti. Parasitology 83, 459–75.CrossRefGoogle Scholar
Wilson, P. A. G., Steven, G. E., & Simpson, N. E., (1982). Strongyloides ratti(homogonic): the time-course of early migration in the generalized host deduced from experiments in lactating rats. Parasitology (in the Press).CrossRefGoogle ScholarPubMed
Zamirdin, M., & Wilson, P. A. G., (1974). Strongyloides ratti: relative importance of maternal sources of infection. Parasitology 69, 445–53.CrossRefGoogle ScholarPubMed