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Passive immunization of mice against Schistosoma mansoni with an IgM monoclonal antibody

Published online by Cambridge University Press:  06 April 2009

M. A. Smith ,
J. A. Clegg ,
D. Snary  and
A. J. Trejdosiewicz
M. A. Smith
Affiliation:
Departments of Parasitology and immunochemistry, Wellcome Research Laboratories, Langley Court, Beckenham, Kent
J. A. Clegg
Affiliation:
Departments of Parasitology and immunochemistry, Wellcome Research Laboratories, Langley Court, Beckenham, Kent
D. Snary
Affiliation:
Departments of Parasitology and immunochemistry, Wellcome Research Laboratories, Langley Court, Beckenham, Kent
A. J. Trejdosiewicz
Affiliation:
Departments of Parasitology and immunochemistry, Wellcome Research Laboratories, Langley Court, Beckenham, Kent
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Summary

Two hybridomas secreting monoclonal IgM antibody to Schistosoma mansoni have been isolated following fusion of spleen cells from Balb/c mice immunized with living S. mansoni and NS1 myeloma cells. One monoclonal IgM antibody (WP66.4) mediated about the same level of passive protection against a challenge infection as immune serum from mice with a chronic S. mansoni infection. The other monoclonal antibody (WP66.2) did not give a significant level of passive protection. This result indicates that the effective monoclonal antibody recognizes an antigen which may be a valuable candidate for experimental vaccination. In vitro one monoclonal antibody (WP66.4) caused a much higher level of complement-dependent cytotoxicity than the other (WP66.2), suggesting a possible mechanism for the effect observed in vivo. With indirect immunofluorescence both monoclonal antibodies reacted with surface determinants on living S. mansoni schistosomula, adult worms and miracidia but these determinants were not detected on cercariae or lung schistosomula. Neither monoclonal antibody cross-reacted with S. haematobium schistosomula or Fasciola hepatica metacercariae, indicating a possible use for these reagents in differential diagnosis of S. mansoni infections.

Type
Research Article
Information
Parasitology , Volume 84 , Issue 1 , February 1982 , pp. 83 - 91
Copyright
Copyright © Cambridge University Press 1982

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