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Daily egg production of Ascaris lumbricoides: the distribution of eggs in the faeces and the variability of egg counts

Published online by Cambridge University Press:  06 April 2009

B. Sinniah
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

Summary

Fifteen children aged from 6 to 12 years who were infected with Ascaris lumbricoides were selected for the study. The number of eggs/g (e.p.g.) of stool was determined using Beaver's direct smear technique, Stoll's dilution egg count technique and Katz's modified thick smear technique. Beaver's direct smear method consistently gave a higher e.p.g. than the other two methods. Results of this study show that Katz's modified thick smear technique was the best of the three methods used. The e.p.g. depends on several factors: the amount of stool passed daily, the concentration of eggs within a stool sample, the daily egg output, the worm load, the age of the worm and the technique used for determining the egg load. The daily average egg output/female A. lumbricoides was estimated to be 238722 (range 134462–358 750) with an average e.p.g. of 3540. The estimation of worm load based on egg count is useful in determining the intensity of infection in an individual. The male/female worm ratio was estimated to be 1: 1·4.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1982

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References

REFERENCES

Augustine, D. L., Nazmi, M., Helmy, M. & McGavran, E. G. (1928). The ova parasite ratio Ancylostoma duodenale and Ascaris lumbricoides. Journal of Parasitology 15, 4551.CrossRefGoogle Scholar
Beaver, P. C. (1949). Quantitative hookworm diagnosis by direct smear. Journal of Parasitology 35, 125–35.CrossRefGoogle ScholarPubMed
Beaver, P. C. (1950). The standardisation of faecal smears for estimating egg production and worm burden. Journal of Parasitology 36, 451–6.CrossRefGoogle ScholarPubMed
Belding, D. L. (1975). Basic Clinical Parasitology. (Revised by Brown, H. W..) 4th edition. New York: Appleton-Century Crofts.Google Scholar
Brown, H. W. & Cort, W. M. (1927). The egg production of Ascaris lumbricoides. Journal of Parasitology 14, 8890.CrossRefGoogle Scholar
Delgado, R., Garnica, Y. & Martinez-Murray, R. (1970). L'irregularite de la ponte d'Ascaris lumbricoides. Annales de Parasitologie 45, 223–6.Google Scholar
Farid, Z., Bassili, S., Wissa, J. & Omar, M. S. (1966). Single dose treatment for Ascaris infection with piperazine citrate; with a study of the egg parasite ratio. American Journal of Tropical Medicine and Hygiene 15, 516–18.CrossRefGoogle ScholarPubMed
Faust, E. C. & Russel, P. F. (1964). Clinical Parasitology. 7th editionPhiladelphia: Lea and Febiger.Google Scholar
Katz, M., Chaves, A. & Pellegrino, J. (1972). A simple device for quantitative stool thick smear technique in Schistosoma mansoni. Revista da Instituto de Medicina Tropical de Sao Paulo 14, 397400.Google Scholar
Komiya, Y. & Kobayashi, A. (1966). Evaluation of Kato's thick smear technique with a cellophane cover for helminth eggs in faeces. Japanese Journal of Medical Science and Biology 19, 5964.CrossRefGoogle Scholar
Krupp, I. M. (1961). Effects of crowding and of superinfection on habitat selection and egg production in Ancylostoma caninum. Journal of Parasitology 47, 957–61.CrossRefGoogle ScholarPubMed
Martin, L. K. (1965). Randomness of particle distribution in human faeces and the resulting influence on helminth egg counting. American Journal of Tropical Medicine and Hygiene 14, (5), 747–59.CrossRefGoogle ScholarPubMed
Mello, D. A. (1974). A note on egg production of Ascaris lumbricoides. Journal of Parasitology 60, 380–1.CrossRefGoogle ScholarPubMed
Stoll, N. R. (1923). Investigation on the control of hookworm disease. An effective method of counting hookworm eggs in faeces. American Journal of Hygiene 3, 5970.Google Scholar
Stoll, N. R. & Hausheer, W. C. (1926). Accuracy in the dilution egg counting method American Journal of Hygiene 6, 80133.Google Scholar
Suzuki, R. & Sanbe, T. (1977). Evaluation of Katz's quantitatve method and its improvement. Japanese Journal of Parasitology 26, 35.Google Scholar
Swellengrebel, N. H. & Sterman, M. M. (1961). Animal Parasites in Man. London: D. Van Nostrand Company.Google Scholar
Yazima, F. & Machinda, K. (1958). On the ecological relations of parasite state of Ancylostoma caninum especially on the density effect of population. Japanese Journal of Parasitology 7, 631–40.Google Scholar