In a series of five irrigated cotton sowings (T2, T7, T9, T11, T14) evapotranspiration (Et) was determined for the period between October 1961 and October 1962 by observing frequently the changes in soil moisture storage, calculating through drainage, and solving for evapotranspiration in the water balance equation. Thus a water balance was obtained for each sowing extending over the entire crop.

The average evapotranspiration in wet season sowings was of the order of 6·5 mm day−1 and in dry season sowings of the order of 4·5 mm day−1. The highest evapotranspiration values ranged between 10 and 12 mm day−1 in T2, T7 and T9 and between 7 and 9·5 mm day−1 in T11 and T14.

1. Inaccurate results previously obtained with wet-combustion methods for the determination of carbon in soils have probably been due to: (a) incomplete oxidation of carbon; (b) incomplete absorption of carbon dioxide. The method described herein ensures complete oxidation and complete absorption. The oxidizing agent employed consists of a mixture of sulphuric acid and potassium bichromate; the absorption takes place in a closed system.

2. When highly accurate results are required, a combustion tube is a necessary adjunct to the wet-combustion method, as appreciable amounts of carbon escape complete oxidation in reaction flask.

3. The soil should be moistened with water before adding the oxidizing mixture, to avoid the formation of complexes, which may protect part of the carbon from oxidation by the acid.

4. Within limits, the concentrations of sulphuric acid and potassium bichromate do not affect the results.

5. While it is necessary to use catalysts (selenium and potassium sulphate) for the complete oxidation of the resistant carbon of coal, catalysts were found to be unnecessary for the determination of carbon in soils.

6. For soils rich in chloride, it is necessary to absorb the chlorine evolved by means of sodium bisulphite solution.

The absorption and movement of 14C-labelled maleic hydrazide (2000 mg/1) and 2,4-D (250 mg/1) were studied in seedlings and mature plants of a local strain of onion (Allium cepa L.) from Kano, Nigeria, in the presence and absence of N (20 mg/1).

Plants absorbed and accumulated considerable amounts of both the chemicals. Addition of N to the treatment solution reduced the absorption of 2,4-D. Also, N reduced the mobility of the chemicals from the roots to the tops which resulted in accumulation of the chemicals in the bulbs.

Physiological problems in some Fife dairy herds were thought to be associated with mineral imbalance in grass cut for silage. Consequently trials at two sites were conducted to study the effects of three levels of nitrogen and phosphorus and two levels of potassium on yield and composition of pasture cut for silage.

Nitrogen increased yield of herbage and reduced its clover content whereas phosphorus and potassium had little effect on yield or clover content. Herbage clover content and calcium concentration declined over the period of the experiment. Mean concentrations of calcium, magnesium and phosphorus were 5·8, 1·7 and 3·4 g/kg respectively. Concentrations of these minerals varied more with time of cutting and with season than with level of applied phosphorus and potassium.

Nitrogen tended to reduce calcium concentration in mixed herbage and to increase magnesium concentration in the grass component; it did not affect herbage phosphorus concentrations, but narrowed the calcium-phosphorus ratio in the second cuts. Applied phosphorus increased herbage phosphorus concentration only at the site with the lower soil phosphorus status. Potassium tended to reduce magnesium concentration in clover and, at one site, in mixed herbage; it also tended to reduce herbage phosphorus concentrations in second cuts.

Mineral uptake was increased by applied nitrogen. Mean annual uptake of calcium magnesium and phosphorus was 34, 10 and 20 kg/ha respectively.

The effects of treatments on concentration and balance of these minerals are discussed in relation to ruminant requirements and maintenance of suitable levels of the minerals in the soil.

It is concluded that normal applications of NPK fertilizer are not detrimental to satisfactory mineral balance in pasture.

Data for the quantity of ammo acids consumed and their flow rates at the proximal duodenum for 33 diets fed to sheep have been statistically analysed by both univariate and multivariate methods. Analysis of variance of net loss (g/day) between mouth and duodenum of individual and total acida was not conclusive. Canonical variate analysis which takes regard of the correlations between different acids both within and between diets provided a conoise method of discriminating between diets. The advantage of this method in generating a unified account of digestion within the rumen is that it effectively reduces the problem from one of many dimensions to a situation where only the first few transformed variables need to be considered.

Nitrosoguanidine-induced mutation frequencies for resistance to streptomycin, spectinomycin, erythromycin and novomycin were studied in Azospirillum brasilense. Lentil inoculated with A. brasilense and its mutants and Rhizobium strains produced increased nodule dry weight, nitrogenase activity of nodules and roots and grain yield compared with an uninoculated control.

Conscious selection is the prime means of keeping the useful qualities of domestic animals at the level they have attained, and of improving them. Valuable economic qualities mostly show the polygenic type of inheritance and therefore continued selection can improve them by sorting out those genes in a herd or breed which are.most desirable for the breeder At the presenttime there is probably not one breed in existence with quantitative characters brought to the homozygous condition. It is the great genetic variation present in breeds which enables selection to be effective in bringing out desirable economic characters.

Water buffaloes (Bubalus bubalis) are reared in many tropical and subtropical countries for meat and milk production and to serve as draught animals. The improvement of milk yield in water buffaloes has been handicapped by the absence of physiological data that may provide means of rapid genetic improvement. Endocrine function is known to vary with lactation intensity. Thyroid hormones seem to have a stimulatory effect on the metabolic rate and, consequently, on the productivity of dairy animals (Lenon & Mixner, 1957; Picha, Pichova & Karasek, 1970; Swanson, 1972). Anderson (1969) also reported an increase in thyroid activity in lactating cows and rats, although other investigators (Johnson & Vanjonack, 1976; Hart et al. 1978; Hart, Bines & Morant, 1979) reported that lactation induced a decline in plasma thyroid hormones. None of these studies performed measurement of plasma thyroid stimulating hormone.

Six species and subspecies of rats and mice, Arvicanthis niloticus, Rattus norvegicus, R. rattus frugivorus, R. rattus alexandrinus, R. rattus rattus, and Mus musculus, were found associated with cotton, wheat, maize, sugar-cane, vegetables, fruit and berseem in the vicinity of Alexandria, Egypt. A. niloticus comprised 42·2% of all species collected. Maize, wheat, cotton and berseem were infested most. The lowest infestation was recorded in the fruit fields. Infestation in sugar-cane and vegetables was intermediate. R. norvegicus, M. musculus, R. rattus frugivorus, R. rattus alexandrinus and it. rattus rattusformed 23·9, 16·7, 10·4, 4·2 and 2·7% of the species collected respectively. Of the seven crops investigated, only two (cotton, wheat) were not infested with R. rattus rattus, but all were plagued with some species of rodents. The abundance of these rodents in fields seemed to be positively correlated with temperature and relative humidity. Winter represents a definite off-season for all species recorded.

Six species of fleas (Xenopsylla cheopis, Nosopsyllus fasciatus, Ctenocephalides felis felis, Leptopsylla segnis, Pulex irritans and Echidnophaga gallinacea), three of sucking lice (Polyplax abyssinica, P. spinulosa and Hoplopleura capitosa), five of parasitic mites (Ornithonyssus bacoti, Laelaps echidnina, L. keegani, Androlaelaps zulu and Haemolaelaps sp.) and the tick Rhipicephalus sanguineus sanguineus were recovered from the rodents. Fleas (especially X. cheopis) and lice generally showed a certain degree of host specificity. A close relationship has been shown to exist between the abundance of fleas and lice and the abundance of some rodent hosts and climate. However, no definite host specificity or relation to climate was shown by infestation with mite species. Only B. sanguineus sanguinetie was recovered from S. rattus frugivorus during July, October and December; from R. rattus alexandrinus during November; and from R. rattus rattus during September. Its rate of infestation did not exceed one specimen per rodent host.

1. An accurate method of estimating the density of spermatozoa in a suspension by the haemocytometer is described.

2. A rapid method of estimating the density of a semen sample by the use of Brown's opacity tubes is given. There is a simple linear relation between opacity and density, the latter expressed in terms of millions of spermatozoa per ml. being approximately five times the opacity standard.

3. The rapid method is sufficiently accurate for use in insemination centres.

Over a period of 3 years 18 autumn-calving, single suckled, April-weaned cows were stocked at three rates at pasture to gain 132, 70 or 25 kg (H, M and L respectively) between April and August and fed to lose 100, 54 or – 13 kg (H, M and L respectively) while housed between November and April, the relative total energy intake over winter being 0·64, 0·83 and 1·00 respectively. Milk yield was reduced by 2·68 kg/day on the H and 1·25 kg/day on the M treatment and as a result calves gained 37 and 10 kg less respectively over winter than on the L treatment. However, despite poorer calf performance economic considerations would indicate a preference for high weight gain during the grazing season and a corresponding loss over winter.

A low phosphorus diet given to growing sheep in metabolism cages was supplemented with different sized fractions of either defluorinated phosphate or dicalcium phosphate. For material of particle size between 0·50 and 0·25 mm diameter, significantly less fluorine was present in the urine and more in the faeces of sheep given defluorinated phosphate than for those given dicalcium phosphate. The fluorine present in coarser defluorinated phosphate was of similar availability to that smaller than 0·50 mm. Finer grade defluorinated or dicalcium phosphates smaller than 0·25 mm diameter were not more biologically available in respect of either fluorine or phosphorus than those of particle size 0·50 to 0·25 mm. The availability of the phosphorus in defluorinated phosphate was reduced when material coarser than 0·50 mm was given to the sheep.

Variations in floral morphology have been observed by most breeders involved in cowpea (Vigna unguiculata (L.) Walpers) improvement. Usually, the most successful pod set is achieved following hand pollination when plants with large flowers are used as female parents because these are easy to manipulate. In breeding work involving wide crosses, unconscious selection of female parents on the basis of flower size could lead to correlated changes in pod and seed characteristics which may or may not be desirable. This would depend on the heritability of flower size and its correlation with the pod and seed characteristics.

The probable error on one pig calculated on the average daily live weight increase was found to be for cross-bred pigs about 7 per cent.; for pure-bred pigs from the same litter and each of similar live weight about 3 per cent.; for cross-bred pigs each of similar live weight but from different parents about 4 per cent.

The error is greatest in experiments of short duration and it decreases as the period of the experiment is lengthened. The decrease is slight if the experiment is prolonged beyond seven weeks.

The errors on 18 separate pens containing four pigs to each pen (the total initial live weight of each pen being similar) varied from 2·3 per cent, to 12·08 per cent., the average being 6·21 per cent.

The percentage probable error is relatively low when the pigs are fattening rapidly and high when the pigs are fattening slowly. The average daily increase of the quicker fattening female pigs when un-speyed appears to be depressed as a result of periodic sexual disturbances.

The stage of fattening does not appear to influence the probable error. Animals in the later stages of fattening show a greater range in their rate of fattening than animals in the early stages, see Fig. 2, p. 284.

Weekly weighing of individual pigs is recommended as it gives an indication of the health of each pig. It also enables each experimenter to discriminate between differences in the rate of fattening due to individuality and differences due to ill health, etc.

The rate of fattening of pigs is very variable, periods of rapid fattening are followed by periods of depression.

When maize forms a large part of the diet of any group of people, pellagra can be prevented by increasing the amount of nicotinic acid or by increasing the amount of tryptophane in the diet. The possibility of increasing the nicotinic acid content of maize by genetical means presents a promising field.

1. The findings presented in two previous papers on the yields of volatile fatty acids, obtained by the action of rumen liquor in the artificial rumen, from fresh grass, dried grass and the water-soluble and water-insoluble separates of the latter, have been amplified by a consideration of the acids similarly obtained from specimens of chemically prepared crude fibre and cellulose, from four of the dried grass specimens.

2. The proportions of different volatile fatty acids from grass crude fibre and grass cellulose resemble those obtained from cellulose powder, propionic acid being produced in greatest relative yield.

3. A general review of these latter findings, in relation to those already presented, has been given.

In a former number of this Journal (1), there appeared a report on the mechanical analysis of soils by this sub-committee of the Agricultural Education Association. The report contained proposals for certain modifications of the earlier A.E.A. (1906) method, of which the most important were the introduction of hydrogen peroxide as a dispersive agent in the preliminary treatment, the use of the pipette method for the actual mechanical analysis, and the abolition of the fine gravel (3 mm.–1 mm.) fraction. This report was officially adopted by the A.E.A. and the method of mechanical analysis therein proposed was accepted as the new standard to replace the 1906 method. The full details were published in the Journal of the Association (2).