Brody (1945) concluded that the inflection point of the growth curve of domestic animals coincides with the onset of puberty and is coupled with an increase in proportion of fat gain at that time. This purported coincidence of growth inflection and onset of puberty has been termed the ‘Brody Law’. Recent findings suggesting a pivotal role of body energy reserves, communicated by the metabolic hormone leptin, on the onset of puberty led to the hypothesis that sheep must reach the inflection point of growth (AIP) considerably before the onset of puberty (AOP). In order to test this hypothesis, growth curves were fitted for ewe lambs on different growth trajectories from two experiments. Both experiments examined the effect of growth trajectory on AOP in ewe lambs. One data set was developed in France with Merino sheep; the other came from two distinct genetic lines of Targhee sheep in the USA. The French experiment subjected ewe lambs to two different feeding levels, while the USA experiment compared two nutritional regimens differing in both energy and protein concentration.

Several non-linear models described in the literature as potentially useful for modelling weight–age relationships were fitted. The Logistic function was identified as the superior model for all datasets. All animals in both experiments reached AIP considerably before AOP. AOP was defined using two criteria threshold levels of progesterone as AOP1 ⩾0·5 ng/ml and AOP2 ⩾1 ng/ml progesterone. For the USA data, multivariate analysis of AIP, AOP1 and AOP2 demonstrated that nutritional treatment was highly significant; this was also the case for the multivariate analysis of AIP, degree of maturity at AOP1 (DOM1) and DOM2. The correlation between AIP and DOM was highly negative. In contrast, the feeding treatment in the French experiments had no effect on any of the response variables except estimated mature weight. However, AIP was negatively correlated with DOM1 and DOM2. AOP was highly positively correlated with DOM. Most notably, AIP was not correlated with AOP1 or AOP2 in either experiment, and all animals reached AIP considerably before AOP1. These findings are consistent with the hypothesis that growing female sheep must reach a certain minimum level of body fatness before the onset of puberty. Genetic group and nutritional treatment significantly affect growth curve parameters; hence, the age at which this (currently unknown) level is reached must strongly depend on nutritional regimen. It is concluded that growth modelling per se cannot be used to infer onset of puberty, and that endocrine thresholds do not necessarily demarcate distinct phases of the growth curve.

Cull cows account for a large part of beef consumption in France and are a significant proportion of farm income for dairy (0·10) and beef systems (up to 0·30). On-farm observations highlight considerable variations in cull cow phenotypes in terms of age, frame size, health, physiological status and body condition. Consequently, an important issue for producers of cull cows is the management of feed supply during the finishing period to obtain a satisfactory condition score and conformation prior to slaughter.

New feeding recommendations for cull cows should consider live weight and live weight gain, age, frame size and body condition score (BCS) to estimate the energy requirements. A predictive model, called CompoCow, was developed for this purpose. The present paper describes the CompoCow model by summarizing developments from previous modelling approaches and outlining the assumptions and equations used in the model. CompoCow combines a growth model for the cow during its productive period (3–8 years old) and a model for the finishing period (in days) and was parameterized for Charolais, Limousine and Holstein breeds. Sensitivity analysis highlighted that the outputs of the model were mainly sensitive to initial body weight and expected body weight gain of the animal. The proportion of lipid in live weight gain was related to body weight, BCS and frame size of the animal. The model also accounts for the higher proportion of lipid in live weight gain in Holstein than in Charolais cows. The model was applied to data from Charolais cows. It showed that the proportion of variability explained by CompoCow for energy requirements was higher than the proportion obtained with previous INRA recommendations, 0·78 and 0·67, respectively. CompoCow does not rely on mechanistic relationships, but it appears robust as it accounts correctly for the effects of age and BCS on the requirements.

Sensitivity analysis is routinely carried out in the evaluation of simulation models to identify the degree to which parameters influence model outputs. This type of sensitivity analysis is much less frequently applied to real systems, but a technique called metabolic control analysis (MCA) was developed in the 1970s for the purpose of experimentally identifying the degree to which individual enzymes in a metabolic pathway influence flux through the pathway. MCA is applied to the results of inhibition, activation or genetic manipulation of enzymatic steps in a biochemical pathway. Flux control coefficients for each enzyme are defined as the fractional change in steady-state flux through the entire pathway for an infinitesimal change in the activity of that one enzyme. The sum of control coefficients in a linear, non-branching pathway is equal to one. It is a common finding in MCA that the control, or sensitivity, is distributed over multiple enzymes and not in a single rate-limiting enzyme. The fundamental principles of MCA are reviewed and an overview of experimental methods to measure control coefficients is provided, with the objective of introducing this approach to the fields of agricultural biochemistry and modelling, where it is little known. The application of MCA to the study of glucose metabolism and fatty acid synthesis in bovine mammary tissue are reviewed. The analyses indicated that mammary hexokinase activity exerts more control than transmembrane transport of glucose over lactose synthesis, and that control of cytosolic fatty acid synthesis is shared between acetyl-CoA carboxylase and fatty acid synthase, contrary to the widely held view that acetyl CoA carboxylase is the rate-limiting enzyme. It is suggested that MCA could be a valuable aid in the integration of proteomic and metabolomic data with metabolic flux measurements to engineer desired changes in the composition of milk from dairy animals.

The effects of various gibberellin-insensitive semi-dwarfing (Rht) alleles and background genotypes on the growth response of wheat seedlings to simulated low water potential were investigated. Four sets of near-isogenic lines, each consisting of six members (Rht-B1a+Rht-D1a (rht), Rht-B1b, Rht-B1c, Rht-D1b, Rht-B1b+Rht-D1b and Rht-B1c+Rht-D1b), and one set of five members (rht, Rht-B1b, Rht-B1c, Rht-B1d and Rht-D1b) were germinated in the presence of polyethylene glycol (PEG). The growth responses were assessed by measuring the lengths of the longest root, coleoptile and longest leaf (shoot) and calculating the root length:shoot length ratio and a tolerance index (TI). Seedling growth was significantly affected by the allelic status at the Rht loci, background genes and the water potential. The PEG treatment had major effects on root and shoot growth. Coleoptile growth was mainly affected by the Rht alleles. There were significant interactions of the Rht allele and variety on the growth response to low water potential. Genotypes with longer roots, coleoptiles and shoots when grown in water, as determined by the Rht allelic status (rht, Rht-B1b and Rht-D1b) and varietal background, had the highest TI and maintained this advantage under stress, while genotypes with smaller seedlings (Rht-B1c and Rht-B1c+Rht-D1b) when grown in water were more strongly affected.

The use of secondary traits such as number of ears per plant, grains per ear, the interval from anthesis to silking, leaf senescence and leaf rolling, together with management of water stress and recurrent selection, have permitted a considerable increase in drought tolerance in the CIMMYT maize source germplasm populations Drought Tolerant Population (DTP) and La Posta Sequía (LPS). Inbred lines were extracted from DTP C9 and LPS C7 cycles and then used for generating single and three-ways hybrids. These were evaluated under normal irrigation and managed drought conditions. A weak, and in some cases no longer significant, correlation was found between grain yield and the traits initially used for selection. Most prominently, the relationship between anthesis-silking interval and grain yield became much weaker in these hybrids. Conversely, significant negative correlations were found between tassel dry weight and grain yield. Three-way hybrids involving two DTP lines yielded more than those involving one only, indicating the feasibility of gene pyramiding for drought tolerance. Overall, the results suggested that the relationship between grain yield and secondary traits has been modified due to continuous selection in the LPS and DTP populations. Some long-established secondary traits have become less important, while others have become more relevant. Mean grain weight, previously not used within a drought selection index, was strongly correlated with yield in the present study. The importance of traits related to the availability in C products for the development of ears and grains are discussed. The results indicate that the traits of source organs contribute marginally to drought tolerance; variation of leaf or root traits seems to be less important than variation in tassel parameters for increasing drought tolerance. For ensuring further progress in drought tolerance in maize, the solution might reside in the manipulation of sink organs. It is therefore suggested that selection for even greater number of ears, bigger grains and smaller tassels may help to increase grain yield under water limited environments in the near future. A short discussion on the optimal choice of parental lines for developing hybrids with maximum expression of drought tolerance concludes the paper.

Cultivated tomato has a narrow germplasm base because of several population bottlenecks in the form of founder events, as well as natural and artificial selections that occurred during domestication and evolution of modern cultivars. The F2 of commercial single-cross hybrids, as well as locally well-adapted varieties, provide germplasm for developing recombinant lines and exploiting genetic variability, respectively. The present study aims to discriminate the breeding value of tomato source material, i.e. commercial hybrids or well-adapted varieties, by (i) estimating tolerance to inbreeding of hybrids or estimating heterosis of diallel hybrids between varieties, (ii) determining undesirable traits and (iii) determining general combining ability (GCA) and specific combining ability (SCA) effects from diallel crosses between hybrids and between varieties. Two hybrids and four varieties were assessed. One hybrid showed 0·03 inbreeding vigour, which was not combined with undesirable traits in the F2 generation. However, negative GCA and positive SCA values did not support the hybrid as source material, provided that hybrids with low inbreeding depression, positive GCA and negative SCA correspond to an F2 capable of developing recombinant lines. The assessment of the varieties showed positive GCA and 0·34 heterosis in one variety, indicating agreement between yield and GCA, and that high-yielding varieties may produce high-yielding hybrids. In conclusion, the proposed mating design, taking into account the tolerance to inbreeding for hybrids and the heritability of general worth for both resources, provides a mechanism for ensuring continued improvement in plant performance through plant selection programmes.

Information on the interactions between genotypes and environments for physiological traits of peanut (Arachis hypogaea L.) is limited. The objective of the present study was to evaluate the effects of seasons and genotype×season (G×S) interactions for dynamic growth and development traits of peanut. Fifteen peanut lines varying in maturity duration, seed type and yield level were grown in a field experiment at the Khon Kaen University in Northeast Thailand during the 2002 and 2003 rainy seasons and the 2003 and 2004 dry seasons. Data were recorded on phenological development stages, pod yield and final biomass, and leaf area index (LAI), crop growth rate (CGR), pod growth rate (PGR), partitioning coefficient (PC), pod harvest index (HI), shelling percentage, and specific leaf area (SLA) were determined. Seasonal effects were found for all development and growth traits of the test peanut lines. Crop duration for the dry season was much longer than for the rainy season because of low temperatures during the early growth stage, causing a delay in flowering and a longer period of pod formation. The test peanut lines showed small differences in the duration of vegetative development and pod formation, but varied greatly in the seed filling duration. This period also showed the greatest differential responses to seasons between the peanut genotypes. Crop yields for the 2003 rainy and the 2004 dry seasons were much lower than for the other two seasons because of late leaf spot disease in the 2003 rainy season and cool temperatures at flowering in the 2004 dry season, resulting in poor pod setting, low PGR and low HI. The test peanut lines differed considerably in pod and biomass yields and all the growth traits measured. Significant G×S interactions were also found for all of these traits, though were much smaller than season effect. Regression analyses identified PGR as the dominant physiological trait determining the G×S interaction for pod yield. Exploring marker-assisted selection for this trait is suggested.

The free volatile compounds of two successive vintages of cv. Caiño Tinto, Caiño Bravo and Caiño Longo red wines, together with the volatile compounds released after the enzymatic hydrolysis of their glycosidically bound forms, were identified and quantified by gas chromatography using a flame ionization detector (GC/FID). All these wines possessed the same free volatile compounds; Caiño Longo wines showed the highest concentrations and Caiño Tinto wines the lowest. In all cases, the release of the bound forms of these compounds may contribute to the final aroma, from both a qualitative standpoint (with the appearance of free 4-terpineol, nerol and geraniol) and quantitative standpoint (notable increases were recorded for most of the compounds detected). The principal component analysis (PCA) showed a good separation of the different wine cultivars and vintages. Caiño Tinto wines were more homogeneous between vintages than the others.

Grassland forbs are important constituents of swards under low-input management. The hypothesis tested was that herbage from unfertilized swards consisting of a mixture of perennial ryegrass (Lolium perenne), white clover (Trifolium repens), dandelion (Taraxacum officinale) and ribwort (Plantago lanceolata) (treatment GCF) would exert a significant effect on the composition and quality of silage for rumen microbes compared with herbage from nitrogen-fertilized (50 kg N/ha/cut) pure ryegrass swards (treatment GN) or unfertilized ryegrass white clover swards (treatment GC). GCF had average proportions of clover and forb of 0·21 and 0·58, respectively. The crude protein and fibre fractions declined and non-fibrous carbohydrates (NFC) increased from GN over GC to GCF. Irrespective of cutting date (spring and summer), GN showed extremely high ammonia (NH3) concentrations, whereas GCF resulted in the highest concentrations of lactic acid coinciding with the lowest contents of butyric acid and ethanol. The ratios between the soluble and less soluble N fractions decreased in the order GN>GC>GCF. A similar sequence was observed in RUSITEC for the apparent disappearance rates of crude protein and fibre fractions. The concentration of NH3-N in the fermenter fluid declined in the order GN>GC>GCF. GCF produced less short-chain fatty acids (SCFA) than GN and GC. Microbial nitrogen (MN) flow was not significantly different between treatments but tended to decline in the order GN>GC>GCF, whereas the proportion of MN originating from NH3-N increased inversely. The efficiency of microbial protein synthesis was unaffected by the different silages. It is concluded that forb-rich swards are suitable for the preparation of high quality silages. There is a need for further investigation into postruminal availability and suitable supplementation for such silages in order to fully exploit their potential for improving the efficiency of nitrogen utilization in ruminant nutrition.

An isotope dilution method using [1-13C]leucine (Leu) infusion together with open-circuit calorimetry was applied to determine the effect of metabolizable protein (MP) intake on rates of plasma Leu turnover and whole body protein synthesis (WBPS) in six heifers. WBPS rate was estimated from rate of plasma Leu turnover and Leu oxidation to carbon dioxide. The experiment consisted of three levels of MP intake and was conducted in a two 3×3 Latin square designs of three 21-day periods. The experimental diet consisted of mixed hay, maize and soybean meal. Dietary MP intake of each dietary treatment was 4·3, 4·5 and 4·9 g/kg BW0·75/day by changing maize and soybean meal weights. Metabolizable energy (ME) intake was similar for all dietary treatments. When plasma α-[1-13C]keto-isocaproic acid enrichments were used as markers indicating intracellular Leu enrichments, plasma Leu turnover rate (LeuTR) increased (P=0·012) and WBPS tended to increase (P=0·091) as MP intake increased. In contrast, plasma LeuTR and WBPS were not influenced if plasma [1-13C]Leu was taken to indicate intracellular Leu enrichments. Total and plasma Leu oxidation rates did not change but intracellular Leu oxidation increased (P=0·044) with increasing MP intake. In heifers, it is suggested that rates of plasma Leu turnover and WBPS are influenced by dietary MP intake, independent of ME intake, although the change in MP intake was relatively small.

The impact of genotype and of frequency and timing of shearing, on mohair attributes and production of modern Angora goats was studied. Goats in the southern hemisphere grazed pastures between February 2004 and 2006. There were seven shearing treatments by three genetic strains with four or eight replicates of individual goats. Treatments were: three different 6-month shearing intervals and two of 12-month shearing intervals with different months of shearing, a 7-month winter shearing interval and a 3-month shearing interval. Genetic strain was based on sire line: 1·0 South African; 1·0 Texan; and Mixed 0·5 South African and 0·5 Texan. Annual greasy mohair production was 5·08 kg, and average clean fleece production was 4·37 kg. The Angora goats produced an annual clean fleece equivalent to 0·122 of their mean fleece-free live weight which was equal to 0·34 g/kg/day. Measurements were analysed over the period of spring 2004 shearing to spring 2005 shearing, excluding the June–December shearing treatment. Increased frequency of shearing increased fleece growth and affected 13 objective and subjective attributes of mohair that were evaluated including clean washing yield, fibre diameter and fibre diameter variation, incidence of medullated fibres, staple length, fibre curvature, crimp frequency, style, staple definition, staple fibre entanglement and staple tip shape. The direction of these effects were generally favourable and for most attributes the magnitude of the response was linear and commercially important. Each additional shearing resulted in an additional 149 g of clean mohair representing 0·034 of the annual clean mohair production. This increase was associated with a 0·6 cm increase in staple length and 0·32 μm increase in mean fibre diameter. In conclusion, Angora goats shorn less frequently grew less mohair that was more likely to be entangled in spring. Managers of Angora goats should take note of these findings.