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The contribution of the gibberellin-insensitive semi-dwarfing (Rht) genes to genetic variation in wheat seedling growth in response to osmotic stress

Published online by Cambridge University Press:  23 November 2007

S. LANDJEVA ,
V. KORZUN ,
E. STOIMENOVA ,
B. TRUBERG ,
G. GANEVA  and
A. BÖRNER
S. LANDJEVA*
Affiliation:
Institute of Genetics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria
V. KORZUN
Affiliation:
Lochow-Petkus GmbH, PF 1197, D-29296 Bergen, Germany
E. STOIMENOVA
Affiliation:
Institute of Mathematics and Informatics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria
B. TRUBERG
Affiliation:
KWS AG, Grimsehlstr. 31, 37574 Einbeck, Germany
G. GANEVA
Affiliation:
Institute of Genetics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria
A. BÖRNER
Affiliation:
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstraße 3, D-06466 Gatersleben, Germany
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

The effects of various gibberellin-insensitive semi-dwarfing (Rht) alleles and background genotypes on the growth response of wheat seedlings to simulated low water potential were investigated. Four sets of near-isogenic lines, each consisting of six members (Rht-B1a+Rht-D1a (rht), Rht-B1b, Rht-B1c, Rht-D1b, Rht-B1b+Rht-D1b and Rht-B1c+Rht-D1b), and one set of five members (rht, Rht-B1b, Rht-B1c, Rht-B1d and Rht-D1b) were germinated in the presence of polyethylene glycol (PEG). The growth responses were assessed by measuring the lengths of the longest root, coleoptile and longest leaf (shoot) and calculating the root length:shoot length ratio and a tolerance index (TI). Seedling growth was significantly affected by the allelic status at the Rht loci, background genes and the water potential. The PEG treatment had major effects on root and shoot growth. Coleoptile growth was mainly affected by the Rht alleles. There were significant interactions of the Rht allele and variety on the growth response to low water potential. Genotypes with longer roots, coleoptiles and shoots when grown in water, as determined by the Rht allelic status (rht, Rht-B1b and Rht-D1b) and varietal background, had the highest TI and maintained this advantage under stress, while genotypes with smaller seedlings (Rht-B1c and Rht-B1c+Rht-D1b) when grown in water were more strongly affected.

Type
Crops and Soils
Information
The Journal of Agricultural Science , Volume 146 , Issue 3 , June 2008 , pp. 275 - 286
Copyright
Copyright © Cambridge University Press 2007

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