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The effect of nutritional status on protein degradation and components of the calpain system in skeletal muscle of weaned wether lambs

Published online by Cambridge University Press:  01 December 1997

B. C. THOMSON ,
B. J. HOSKING ,
R. D. SAINZ  and
V. H. ODDY
B. C. THOMSON
Affiliation:
Faculty of Agriculture and Forestry, University of Melbourne, Victoria 3052, Australia
B. J. HOSKING
Affiliation:
Faculty of Agriculture and Forestry, University of Melbourne, Victoria 3052, Australia
R. D. SAINZ
Affiliation:
Department of Animal Science, University of California, Davis, CA, USA
V. H. ODDY
Affiliation:
CRC for Meat Quality, University of New England, Armidale, NSW 2351, Australia
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Abstract

The association between the rate of protein degradation and the components of the calpain system in lambs fed at sub-maintenance, maintenance and supra-maintenance levels of nutrition was investigated. Weights of the cold carcass, liver, kidney, pluck and m. semitendinosus increased with nutritional level (P<0·05). The rate of protein degradation in the hind-limb was determined using an in vivo arterio-venous method. Blood flow, protein gain and protein synthesis across the hind-limb increased with nutritional status (P<0·05). There was an increase in the amount of protein synthesised per unit of RNA (mg RNA/g protein) with improved nutritional status. The rate of protein degradation across the hind-limb increased between the sub-maintenance and maintenance treatments (P<0·05) but there was no further increase above maintenance (P>0·10). The activity of the components of the calpain system was determined after separation on a DEAE-Sepharose column with a stepwise gradient with increasing NaCl concentrations. Although there were no significant effects of nutritional status on the components of the calpain system, there was a negative association between the rate of protein degradation and the activity of μ-calpain (R2=0·61; P<0·005) and a weak positive association between calpastatin activity and protein gain (R2=0·44; P<0·05).

Type
Research Article
Information
The Journal of Agricultural Science , Volume 129 , Issue 4 , December 1997 , pp. 471 - 477
Copyright
© 1997 Cambridge University Press

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