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Discrimination of Cricotopus species (Diptera: Chironomidae) by DNA barcoding

Published online by Cambridge University Press:  01 July 2008

C.S. Sinclair  and
S.E. Gresens
C.S. Sinclair*
Affiliation:
Department of Biological Sciences, Towson University, Towson, Maryland, USA 21252
S.E. Gresens
Affiliation:
Department of Biological Sciences, Towson University, Towson, Maryland, USA 21252
*
*Author for correspondence Fax: 1 (410) 704-2405 E-mail: [email protected]
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Abstract

Chironomids (Diptera) typically comprise the most abundant group of macroinvertebrates collected in water quality surveys. Species in the genus Cricotopus display a wide range of tolerance for manmade pollutants, making them excellent bioindicators. Unfortunately, the usefulness of Cricotopus is overshadowed by the difficulty of accurately identifying larvae using current morphological keys. Molecular approaches are now being used for identification and taxonomic resolution in many animal taxa. In this study, a sequence-based approach for the mitochondrial gene, cytochrome oxidase I (COI), was developed to facilitate identification of Cricotopus species collected from Baltimore area streams. Using unique COI sequence variations, we developed profiles for seven described Cricotopus sp., four described Orthocladius sp., one described Paratrichocladius sp. and one putative species of Cricotopus. In addition to providing an accurate method for identification of Cricotopus, this method will make a useful contribution to the development of keys for Nearctic Cricotopus.

Keywords

chironomidCricotopuscytochrome oxidase IbioindicatorDNA barcoding
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 98 , Issue 6 , December 2008 , pp. 555 - 563
Copyright
Copyright © 2008 Cambridge University Press

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