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Time course of changes in in vitro lipolysis of intra-abdominal fat depots in relation to high-fat diet-induced hepatic steatosis in rats

Published online by Cambridge University Press:  08 March 2007

Pascal Collin ,
Natalie Chapados ,
Elise Dufresne ,
Pierre Corriveau ,
Pascal Imbeault  and
Jean-Marc Lavoie
Pascal Collin
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
Natalie Chapados
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
Elise Dufresne
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
Pierre Corriveau
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
Pascal Imbeault
Affiliation:
School of Human Kinetics, University of Ottawa, 125 University Street Ottawa, KIN 6N5, Ontario, Canada
Jean-Marc Lavoie*
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
*
*Corresponding author: Dr Jean-Marc Lavoie, fax +1 514 343 2181, email: [email protected]
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Abstract

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The purpose of the present study was to determine the time course of changes in in vitro lipolysis and in perilipin content (Western blot) in the mesenteric and/or the retroperitoneal fat depots in relation to the development of hepatic steatosis in high-fat diet-fed rats. Female Sprague-Dawley rats were submitted to a high-fat diet (HF diet; 42% as kJ) or a standard diet (SD diet) for 1, 2, 3 or 8 weeks. Fat accretion in the mesenteric and retroperitoneal tissues was higher (P<0·01) in HF diet-fed than in SD diet-fed rats as soon as 1 week after the beginning of the diet. Liver triacylglycerol concentrations were significantly (P<0·01) higher in HF diet-fed than in SD diet-fed rats throughout the experiment, the highest values being reached at week 2 of the diet. Basal and stimulated lipolysis (10−4 to 10−7m-isoproterienol) in the mesenteric and retroperitoneal fat depots was not changed during the first 3 weeks, regardless of the diet. Lipolysis in the mesenteric adipose tissue in the basal and stimulated states was, however, higher (P<0·01) in HF diet-fed than in SD diet-fed rats after 8 weeks of the diets. There were no significant (P>0·05) effects of diet and time on perilipin content of mesenteric tissue. In spite of a rapid fat accretion, the present results do not provide any evidence of a rapid (3 weeks) increase in in vitro lipolysis in intra-abdominal fat depots upon the undertaking of an HF diet at a time where liver lipid infiltration is the most significant.

Keywords

PerilipinPlasma NEFALeptinHepatic steatosisIsoproterenol-induced lipolysis
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 2 , August 2006 , pp. 268 - 275
Copyright
Copyright © The Nutrition Society 2006

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