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Quantification of the absorption of nutrients derived from carbohydrate assimilation: model experiment with catheterised pigs fed on wheat- or oat-based rolls

Published online by Cambridge University Press:  09 March 2007

Knud Erik Bach Knudsen ,
Henry Jørgensen  and
Nuria Canibe
Knud Erik Bach Knudsen*
Affiliation:
Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
Henry Jørgensen
Affiliation:
Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
Nuria Canibe
Affiliation:
Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
*
*Corresponding author: Dr Knud E. Bach Knudsen, fax +45 89 99 13 78, email [email protected]
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Abstract

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The main purpose of this study was to quantify the absorption of nutrients derived from carbohydrate assimilation in a model experiment with catheterised pigs. A low-fibre (LF) diet based on wheat flour and two high-fibre diets with added insoluble fibre from wheat bran (HFWB) or soluble fibre from oat bran (HFOB) were used. The diets were offered as baked rolls to three catheterised pigs in a 3×3 Latin square design. The pigs were surgically fitted with catheters placed in the portal vein and mesenteric artery and with an ultrasonic flow probe attached to the portal vein to monitor the blood-flow rate. The pigs were fed the diets three times daily and portal and arterial blood samples collected twice weekly up to 8 h after the morning feeding. Glucose, insulin, lactic acid (LA) and short-chain fatty acids (SCFA) were determined on the samples. The baseline level of glucose in the portal vein was about 6 mmol/l increasing to 10–11 mmol/l 20–30 min post-feeding with no difference among the different diets. Portal and arterial insulin mirrored portal glucose concentration and was also unaffected by the dietary composition. The net absorption of glucose (per 24 h) was: diet LF 4190 mmol; diet HFWB 3050 mmol and diet HFOB 3190 mmol corresponding to a recovery of 0·76–0·92 of ingested starch. The levels of LA and SCFA in the portal vein were relatively constant in the postprandial period. The net absorption of LA and SCFA was in the same order (749 and 720 mmol/d respectively) with diet LF, while LA was lower (384 and 582 mmol/d) and SCFA higher (738 to 891 mmol/d) when feeding the two high-fibre diets. There was a higher molar proportion of butyrate in the portal vein after feeding the high-fibre diet supplemented with oat bran as compared with the wheat-based diets.

Keywords

Catheterised pigsAbsorptionGlucoseLactic acidShort chain fatty acids
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 4 , October 2000 , pp. 449 - 458
Copyright
Copyright © The Nutrition Society 2000

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