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Effect of temperature, volume of ova batches, and addition of a diluent, an antibiotic, oxygen and a protein inhibitor on short-term storage capacities of turbot, Psetta maxima, ova

Published online by Cambridge University Press:  15 July 1999

Marc Suquet
Affiliation:
Laboratoire de physiologie des poissons, Ifremer, Centre de Brest BP 70, 29280 Plouzané, France
Olvido Chereguini
Affiliation:
Centro Oceanográfico de Santander, I.E.O., Apdo 240, 39080 Santander, Spain
Marie-Hélène Omnes
Affiliation:
Laboratoire de physiologie des poissons, Ifremer, Centre de Brest BP 70, 29280 Plouzané, France
Inmaculada Rasines
Affiliation:
Centro Oceanográfico de Santander, I.E.O., Apdo 240, 39080 Santander, Spain
Yvon Normant
Affiliation:
Laboratoire de physiologie des poissons, Ifremer, Centre de Brest BP 70, 29280 Plouzané, France
Isabel Pan Souto
Affiliation:
Laboratoire de physiologie des poissons, Ifremer, Centre de Brest BP 70, 29280 Plouzané, France
Loïc Quemener
Affiliation:
Laboratoire de physiologie des poissons, Ifremer, Centre de Brest BP 70, 29280 Plouzané, France
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Abstract

The effect of different parameters on short-term storage capacity of turbot ova was assessed over a 45-h period after ova collection for fertilization rates and over a 9-h period after ova collection for hatching rates. Increasing the volume of ova sampling from 0.5 to 2.5 mL, as well as adding an antibiotic-antimicotic solution or oxygen did not significantly change the storage capacity of ova. Regarding the hatching rates, a higher storage ability was recorded at 8 and 13 °C, compared to 3 °C. The mean composition of the ovarian fluid was determined (n = 57 spawns). Use of a diluent mimicking the ovarian fluid significantly decreased the storage ability as assessed by the fertilization rates but did not modify the hatching rates. Diluting ova in an artificial ovarian fluid deprived of calcium significantly decreased the fertilization and hatching rates during the storage period. Furthermore, addition or not of soybean trypsin inhibitor (Sigma T 9003) to the artificial ovarian fluid deprived of calcium did not significantly change the results. Storage capacity of control batches of ova was low: at 13 °C, without any diluent and when ova were fertilized 3 h after stripping, the hatching rate was lowered to 62.4 ± 29.4 % (mean ± SD) of the initial value.

Type
Research Article
Copyright
© Elsevier, IRD, Inra, Ifremer, Cemagref, CNRS, 1999

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