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Analysis of the adaptation to alkanes of the marine bacterium Marinobacter hydrocarbonoclasticus sp 17 by two dimensional gel electrophoresis

Published online by Cambridge University Press:  15 October 2004

Guillaume Ballihaut ,
Benjamin Klein ,
Philippe Goulas ,
Robert Duran ,
Pierre Caumette  and
Régis Grimaud
Guillaume Ballihaut
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Benjamin Klein
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Philippe Goulas
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Robert Duran
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Pierre Caumette
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Régis Grimaud
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
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Abstract

To better understand the molecular mechanisms involved in the biodegradation of hydrocarbon compounds from the “Erika” oil-spill, we have studied the ability of Marinobacter hydrocarbonoclasticus strain sp 17 to cope with hexadecane as sole carbon and energy source. Growth kinetics of cultures shifted from acetate to hexadecane revealed the presence of a 20 hours adaptation phase. Changes in global protein expression in response to hexadecane was analyzed by two-dimensional gel electrophoresis. Of the 370 proteins detected 42 had their expression level altered in presence of hexadecane indicating that alkane adaptation may involve many cellular functions.

Keywords

AlkanesBiodegradationProteomic2D Electrophoresis
Type
Research Article
Information
Aquatic Living Resources , Volume 17 , Issue 3: The "Erika" Oil Spill: Environmental Contamination and Effects in the Bay of Biscay , July 2004 , pp. 269 - 272
Copyright
© EDP Sciences, IFREMER, IRD, 2004

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