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Isolation and use of microsatellite loci in Melicertus kerathurus (Crustacea, Penaeidae)

Published online by Cambridge University Press:  17 March 2010

Marco Arculeo ,
Rosalia Pellerito  and
François Bonhomme
Marco Arculeo
Affiliation:
Dipartimento di Biologia Animale “G. Riverberi”, Università degli studi di Palermo, Via Archirafi 18, 90123 Palermo, Italy
Rosalia Pellerito
Affiliation:
Dipartimento di Biologia Animale “G. Riverberi”, Università degli studi di Palermo, Via Archirafi 18, 90123 Palermo, Italy
François Bonhomme
Affiliation:
Institut des Sciences de l'Evolution, CNRS UMR 5554, Université de Montpellier II, Station méditerranéenne de l'Environnement littoral, 1 quai de la Daurade, Sète 34200, France
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Abstract

Melicertus kerathurus represents an economically important resource for fisheries and aquaculture. Seven microsatellite loci from 373 specimens of M. kerathurus collected in different parts of the Mediterranean and NE Atlantic were identified. Four of these microsatellites showed a moderate level of polymorphism, with 2 to 14 alleles per locus, whereas three had a monomorphic profile. Observed and expected heterozygosity ranged between 0.28 to0.64 and 0.28 to 0.65, respectively. Three microsatellite loci deviated from Hardy-Weinberg equilibrium in some populations, with a deficit of heterozygosity. Mean Fst values showed significant differentiation among sample sites analysed and indicate that these loci are useful for the study of genetic variation in this species. These results may supply functional information on the population genetic structure of Melicertus kerathurus, valuable for future sustainable management strategies.

Keywords

MicrosatellitesGenetic variationPCR–RFLPPenaeid shrimpMelicertus kerathurusMediterranean SeaAtlantic Ocean
Type
Note
Information
Aquatic Living Resources , Volume 23 , Issue 1 , January 2010 , pp. 103 - 107
Copyright
© EDP Sciences, IFREMER, IRD, 2010

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