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DNA sequencing and genetic diversity of the 18S–26S nuclear ribosomal internal transcribed spacers (ITS) in nine Antarctic moss species

Published online by Cambridge University Press:  17 August 2005

M.L. SKOTNICKI ,
A.M. MACKENZIE ,
M.A. CLEMENTS  and
P.M. SELKIRK
M.L. SKOTNICKI
Affiliation:
Genomic Interactions Group, Research School of Biological Sciences, Institute of Advanced Studies, Australian National University, Canberra, ACT 2601, Australia
A.M. MACKENZIE
Affiliation:
Genomic Interactions Group, Research School of Biological Sciences, Institute of Advanced Studies, Australian National University, Canberra, ACT 2601, Australia
M.A. CLEMENTS
Affiliation:
Centre for Plant Biodiversity, CSIRO Plant Industry, Canberra, ACT 2601, Australia
P.M. SELKIRK
Affiliation:
Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia
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Abstract

We have sequenced the 18S–26S nuclear ribosomal DNA ITS region from the genome of nine different moss species from the Ross Sea region of Antarctica. This relatively quick and simple technique enables these species to be readily distinguished, facilitating their taxonomic identification. Only a single moss shoot is required, and for identification of these bryophytes it is only necessary to determine a few hundred nucleotides of the DNA sequence in a single sequencing reaction. Several previously unidentified Antarctic moss specimens were readily characterized by comparison with ITS sequences of known moss species. The relationships between species and locations previously detected by the RAPD (Random Amplified Polymorphic DNA) technique were confirmed by DNA sequencing, demonstrating that the two techniques can be complementary for molecular analysis of the ecology of mosses in Antarctica.

Keywords

bryophyteITS sequencemolecular taxonomymolecular ecologymoss dispersalVictoria Land
Type
Research Article
Information
Antarctic Science , Volume 17 , Issue 3 , September 2005 , pp. 377 - 384
Copyright
© Antarctic Science Ltd 2005

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