Two experiments have been carried out to examine the effects of the level of protein supplementation given with grass silage-based diets on the performance and carcass composition of bulls, and to compare diets based on silage and dried forage. The five treatments used consisted of grass silage offered ad libitum and supplemented with 2·5 kg dry matter (DM) of barley-based concentrates containing (1) zero (2) 200 (3) 400 and (4) 600 g soya-bean meal per kg and (5) artificially dried grass and hay supplemented with 3·2 kg concentrate DM. The silages used in both experiments were well preserved, containing on average 200 g DM per kg; 140 g crude protein (CP) per kg DM; 63 g ammonia-nitrogen per kg total N and 731 g digestible organic matter per kg DM. The bulls were of late-maturing breed type and were initially 12 months old and 412 and 405 kg live weight in experiments 1 and 2 respectively. For treatments 1, 2, 4 and 5 in experiment 1 respectively (treatment 3 was not used) total DM intakes were 8·3, 8·3, 81 and 110 (s.e. 0·21) kg/day; CP intakes 1063, 1271, 1664 and 1539g/day; metabolizable energy intakes (MEI) 98, 99, 96 and 87 MJ/day; carcass weights 317, 316, 317 and 316 (s.e. 3·2) kg; carcass saleable meat concentrations 714, 712, 718 and 716 (s.e. 5·8) g/kg and carcass fat trims 73, 81, 73 and 68 (s.e. 3·9) g/kg. In experiment 2 for treatments 1 to 5 respectively total DM intakes were 8·3, 8·5, 8·3, 8·4 and 11·2 (s.e. 0·26) kg/day; CP intakes were 1090, 1329, 1504, 1720 and 1561 g/day; MEI 102, 106, 103, 103 and 94 MJ/day; carcass weights 318, 331, 330, 327 and 321 (s.e. 3·3) kg; carcass saleable meat concentrations 726, 721, 725, 721 and 732 (s.e. 60) g/kg and fat trims 71, 77, 78, 80 and 64 (s.e. 4·5) g/kg. It is concluded that protein supplementation of a silage-based diet did not affect performance or carcass fatness in experiment 1 or carcass fatness in experiment 2, but including 200 or 400 g soya-bean meal per kg concentrate increased performance in experiment 2. Animals given silage produced fatter carcasses than those given dried forage in experiment 2 but not in experiment 1.

An analysis of cow reproductive traits was carried out on 2057 lactation records of Egyptian buffaloes. A total of 651 daughters (paternal half-sisters) of 82 sires were available for the analysis. Reproductive traits analysed were age at first, second and third calving (AC), number of services per conception (NSC), gestation length (GL) and calving interval (CI). Year of calving affected most reproductive traits (P < 0·001) while no important differences were detected among months of calving. Means of NSC, GL and CI decreased linearly (P < 0·01) as parity advanced. Age of cow at calving exerted a pronounced effect (P < 0·001) on NSC and CI. There was no systematic change in the estimates of heritability and sire components of variance for reproductive measures over the first three lactations. Heritability estimates for NSC and GL in the different parities were generally low, ranging between 0·04 and 0·18, while moderate estimates for AC and CI were obtained. Phenotypic correlations between reproductive traits were positive and relatively moderate or low. For most traits, positive and relatively high or moderate estimates of genetic correlations were observed. Mating bull was found to be an important source of variation for reproductive performance of Egyptian buffaloes. The highly significant effect of mating bull on reproductive traits could be considered as evidence for the importance of evaluating bull semen quality before the mating of cows

Two divergent lines of sheep were produced by selection on an index of testis diameter adjusted for body weight at 6, 10 and 14 weeks of age. There were correlated changes in growth patterns, such that animals in the high and low lines were of a similar weight at birth, but thereafter, the low line became progressively heavier than the high line. After 11 years of selection there was a 7-kg difference between the mean body weight of females in the two lines at 1·5 years of age and a 12-kg difference at 2·5 years. There was little evidence of any accompanying changes in body shape or in carcass composition. High-line females of all reproductive ages had a significantly higher fertility. High-line lambs had a significantly lower mortality and, at the end of selection, had an estimated probability of survival to 210 days of 0·25, 013 and 003 higher than in lambs from the low line, in lambs from 1-, 2- and 3-year-old dams respectively. The estimated number of lambs alive at 210 days per ewe mated was 0·30, 0·38 and 0·34 higher in the high line for ewes of 1, 2 and 3 years of age, but low-line lambs were 3 kg heavier on average at 210 days. The combined impact of improved ewe performance and changes in growth patterns led to a predicted greater production efficiency in the high line. It was estimated that, after 11 years of selection on the index of weight adjusted testis diameter, 0·62, 0·71 and 0·74 kg of high-line ewe at 1, 2 and 3 years of age, respectively, would be needed to produce the same weight of lamb at 210 days as 1 kg of low-line ewe. Improved ewe performance had a much larger impact on the changes in comparative efficiency than changes in growth patterns, but the contribution of the latter increased with age of ewe. Further experimental verification of the line differences in reproduction and lamb survival and study of their causes are needed. Nevertheless, these results indicate the promise of using indices of body weight and testis size to increase the efficiency of lamb production.

Fifty Ethiopian Menz ewes maintained on pasture grazing with uncontrolled mating showed ovarian cyclicity at all times of the year. Conception rates to first, second and third service were 78, 18 and 4%, respectively. Mean post-partum anoestrous interval was 76·1 (s.e. 4·6) days. Two-thirds of ewes conceived before lamb weaning at 90 days and had 8 month lambing intervals. Twice weekly progesterone determinations showed that 67% of ewes exhibited a silent ovulation 66·4 (s.e. 10·5) days post partum, 12% of oestrous cycles were longer than 20 days and 8% of heats were false or anovulatory. It was concluded that Menz ewes have the natural potential for three lambings in 2 years, under adequate nutrition. Their better management and genetic improvement should further reduce current lambing intervals and increase annual reproductive rates.

The aim of this study was to compare responses to cold stress in newborn lambs and kids. Two experiments were conducted in a climatic chamber with controlled air temperatures and artificial wind and rain. The first experiment involved 25 Poll Dorset × (Border Leicester × Romney) lambs and 16 Angora × feral kids while the second involved 30 Suffolk × (Border Leicester × Romney) lambs and 26 Angora × feral kids. Rectal temperatures were measured and the rate of decline in rectal temperature estimated in both experiments. In the second experiment, metabolic rate was also measured and coat characteristics (birth coat depth and wool weight per unit area of skin) were examined. The effects of birth rank and sex on resistance to cold exposure and their interaction with species were examined in both experiments.

In experiment 1, kids had lower rectal temperatures than lambs at the start of the test but there was no species effect on the rate of decline in rectal temperature. In experiment 2, kids exhibited a significantly greater rate of decline in rectal temperature than did lambs. This difference in species effect between the experiments reflected primarily a greater rate of body cooling in the Poll Dorset-cross lambs (experiment 1) than in the Suffolk-cross lambs (experiment 2) rather than a difference between the kids in the two experiments. Additionally, in experiment 2, metabolic rate per unit live weight was significantly lower in the kids than in the lambs. No differences were apparent between the species in coat characters. Multiple-born lambs and kids were found to exhibit a significantly greater rate of decline in rectal temperature than singles, but only marginal differences in heat production were observed between the birth ranks. No significant effects of sex on resistance to cold stress were found in this study. Effects of birth rank and sex were additive with those of species. It is concluded that a combination of low heat production capacity and small body size is responsible for the generally poor responses of newborn kids to cold stress but there is probably little difference between lambs and kids in the insulative value of their birth coats.

Various types of constant-release melatonin implants were designed and administered to adult ewes during seasonal anoestrus. Implants were given between May and July in a series of four experiments. Treatment of ewes with melatonin implants resulted in advancement of the onset of oestrous activity. Melatonin treatment also resulted in improved reproductive performance, increased numbers of lambs born per ewe bred and caused more multiple births. Results from this study show, therefore, that constant-release melatonin implants administered during late spring or early summer advance the onset of the breeding season and improve reproductive performance in ewes.

A series of change-over experiments examined the intake and digestibility of straw-based diets in yearling dairy heifers. Voluntary intakes of straws given as the sole food to yearling dairy heifers were 1·28, 1·20 and 0·90 kg dry matter (DM) per 100 kg live weight for barley, wheat and oat straws respectively, and the corresponding digestibility of organic matter was 0·47, 0·53 and 0·48. When supplemented with 2 kg rolled barley the intakes of straw did not alter, nor were they affected by replacing part of the rolled barley with an equal amount of soya-bean meal and thus increasing the dietary concentration of protein from about 50 to 110 g/kg DM. The use of molassed sugar-beet pulp in place of rolled barley reduced the intake of straw. Flaked maize promoted slightly higher intakes of straw than an equal amount of coarsely milled maize. Replacing rolled barley by lucerne hay reduced straw intake. It is estimated that, to provide sufficient energy and protein for a daily gain of 0·5 kg, heifer straw-based diets would require a supplement of 1·2 kg rolled barley and 0·8 kg soya-bean meal.

The chemical composition of the muscle and fatty carcass tissues of Hereford × Friesian (HE), Friesian (FR) and Charolais × Friesian (CH) steers finished on high (H) (12·6 MJ/kg dry matter) and medium (M) (10·4 MJ/kg dry matter) metabolizable energy (ME) concentration diets and slaughtered at low (L), normal (N) and heavy (W) carcass weights was determined in a 3 × 2 × 3 (no. = 9) factorial experiment. There were also pre-finishing slaughter groups of nine animals of each breed type. Target L, N and W carcass weights were 260, 300 and 340 kg, for HE and FR and 260, 320 and 380 kg, for CH, respectively.

Main effect side soft tissue weights of the finished groups were 128, 125 and 134 (s.e.d. 1·3) kg for HE, FR and CH, 133 and 125 (s.e.d. 1·1) kg for H and M and 108, 129 and 150 (s.e.d. 1·3) kg for L, N and W, respectively. Soft tissue chemical composition was 540, 562 and 590 (s.e.d. 5·0) g/kg moisture, 162, 174 and 181 (s.e.d. 1·7) g/kg protein and 288, 254 and 220 (s.e.d. 6·6) g/kg lipid for HE, FR and CH and 558 and 570 (s.e.d. 4·1) g/kg moisture, 170 and 175 (s.e.d. 1·4) g/kg protein and 262 and 246 (s.e.d. 5·4) g/kg lipid for H and M, respectively. The allometric regression coefficients for moisture, protein and lipid weights on soft tissue weight were 0·65, 0·69 and 206, respectively. The coefficients for moisture and protein weights on muscle and fat weights were < 1·0, whereas for lipid, they were >1·0. The breed differences in soft tissue chemical composition paralleled differences in physical composition. Dietary ME concentration had little effect on the chemical composition of individual tissues. For the M diet, HE, FR and CH were calculated to have similar soft tissue lipid concentrations (250 g/kg) at side soft tissue weights of 113, 125 and 157 kg, respectively. Corresponding side muscle weights at similar muscle lipid concentration (70 g/kg) would be 77, 90 and 108 kg.

The variation in the content of total protein, total casein, whey protein, α-casein, β-casein, k-casein, α-lactalbumin, β-lactoglobulin and ‘other’ whey proteins of the mammary secretion was studied in 44 goats of the Murciano-Granadina breed (Spain) from the 1st to the 4th day after parturition.

The concentration of all the protein variables showed a marked decrease from the 1st to the 4th day after parturition (P < 0·001). In whey proteins this decrease was more marked from the 1st (67·1 g/1) to the 2nd day after parturition (21·6 g/1), whereas the total caseins sharply decreased from the 2nd (62·6 g/1) to the 3rd day after parturition (39·0 g/1).

Stepwise discriminant analysis revealed that, except for the k-casein, all the proteins resulting from the electrophoretic fractionation had a discriminant power between the dates of sampling (P < 0·001). The multivariant analysis did not show statistical differences in the electrophoretic fractions corresponding to the samples on the 3rd and 4th days after parturition. According to such fractionation, the transition from colostrum to milk should take place on the 2nd day after parturition.

Two experiments were conducted each using one batch of six Landrace littermate, entire male, pedigree pigs in a Latin-square change-over study of the effects of energy intake on nitrogen and energy metabolism over the range 33 to 88 kg live weight. One animal from each litter was slaughtered at 33 kg body weight to obtain initial body composition data. Five feeding levels (80, 100, 120, 140 and 160g/kg M0·63) were used during five consecutive metabolism trials each of 11-days duration, excreta being collected during the last 7 days. The pigs were housed in individual metabolism cages and the diets were offered in liquid form (approx. 300 g dry matter (DM) per kg) twice daily at 09.00 and 16.00 h. Heat production was measured for 1 day during each balance period in an open-circuit respiration chamber. The average daily gain, nitrogen retention, heat production and energy retention increased linearly (P < 0·001) with increasing metabolizable energy (ME) intake. The relationship between energy intake and protein deposition was linear up to levels above the normal ad libitum consumption of energy. Protein deposition potential of these high genetic potential pigs was at least 200 g/day, and tended to be constant between 35 and 85 kg live weight. From the combined results of experiments 1 and 2, the energy requirement for maintenance was 0·982 MJ ME per kg M0·63 per day and the decrease in protein deposition was approximately 6 g/MJ reduction in ME within the range of practical energy intakes.

In the Mediterranean environment and subtropical microclimate of the south-east Iberian Peninsula (37° 59' N), testicle size and sexual behaviour of male goats were studied and the influence of season (day length, air temperature and relative humidity) on these factors examined.

Nine, 13-month-old Murciano-Granadina male goats weighing 47 to 53 kg were used. The experiment began during the month of January and extended for a complete year. Every 2 weeks, anterior-posterior maximum testis diameter was measured. They were tested twice a week for levels of sexual behaviour using ovariectomized goats and libido was evaluated as reaction time to mating.

The annual mean testis diameter was 5·06 (s.e. 0·02) cm, varying significantly with season of the year. The lowest values were measured during winter 4·89 (s.e. 0·03) cm. These increased (P <0·01) in spring (5·16 (s.e. 0·01) cm) and summer (5·19 (s.e. 0·02) cm), but decreased again (P <0·01) in the autumn (5·04 (s.e. 0·02) cm). The annual mean reaction time was 197 (s.e. 12) s, but was longest (P <0·01) in spring (318 (s.e. 30) s), and shortest during summer (144 (s.e. 16) s) and autumn (132 (s.e. 17) s). The testicle diameter measurement was correlated with day length (r = 0·41; P <0·01) and air temperature (r = 0·45; P<0·01), while the reaction time showed no relationship with climatic measurements.

It was concluded that day length is not the only factor responsible for seasonal variation in testis size of Murciano-Granadina male goats and that, in their natural habitat, libido is unlikely to be a limiting factor in the breeding programme.

Forty-two weaned suckled Charolais-cross steers were used to measure changes in body composition during compensatory growth in growing cattle. Six cattle were slaughtered initially and the remaining 36 allocated to either a low level of feeding to 350 kg live weight followed by a high level (LH) or a high level of feeding throughout (HH). Above 350 kg live weight, food intake on both treatments was the same at any given live weight. Six cattle were slaughtered from each treatment at 350, 400 and 450 kg live weight. From initial live weight (259 kg) to 350 kg, live-weight gains were 0·45 and 0·78 kg/day for the LH and HH treatments respectively (P < 0·001). From 350 to 400 kg live weight, live-weight gains were 1·35 and 0·98 kg/day (P < 0·01) for the LH and HH cattle respectively, while from 400 to 450 kg live weight there was no significant difference (1·38 v. 1·20 kg/day). The LH cattle contained less fat in the empty body than the HH cattle at 350 kg (118 v. 153 g/kg; P < 0·05) and 400 kg live weight (117 v. 169 g/kg; P < 0·01), but at 450 kg there was no significant difference between treatments. From 350 to 400 kg live weight the composition of the empty body-weight gain was 663 g water, ' 108 g fat and 216 g protein per kg in the LH cattle and 422 kg water, 311 g fat and 173 g protein in the HH cattle. From 400 to 450 kg live weight the equivalent figures were 491, 291, 156 g/kg for the LH cattle and 744, 67 and 203 g/kg for the HH cattle. The results demonstrate that following a period of food restriction the empty body-weight gain of cattle initially comprises increased proportions of protein and water and a reduced proportion of fat compared with unrestricted cattle when both are given the same amount of food and compared at the same weight. There then follows a second phase in which the proportion of fat increases and the proportions of protein and water decrease.

Ground full-fat rapeseed (FFRS) and the resulting extracted oil (RSO), meal (RSM) and recombined RSO and RSM (ORSM) from a triple-low cultivar containing low levels of erucic acid, glucosinolates and tannins were evaluated. RSO, FFRS and ORSM were included into a basal diet to give levels of added oil of 40, 80 and 120g/kg. RSM was also incorporated into the basal diet at the same rates as in ORSM. The 12 experimental diets together with the basal diet were given to 13 gilts in a metabolism trial conducted over four time periods. A 10-day acclimatization in holding pens preceded a 5-day total collection of faeces and urine in metabolism crates. Rapeseed products, diets, faeces and urine were analysed for gross energy (GE) and all, except urine, for oil content measured by two methods (petroleum ether and acid hydrolysis prior to ether extraction). Apparent digestible energy (DE) and metabolizable energy (ME) of rapeseed products were determined by single-level assay and by regression. Oil DE was also derived indirectly as a product of apparent oil digestibility (AOD) and oil GE. Apparent nitrogen digestibility was estimated for diets and rapeseed products. The response of dietary DE, ME and AOD to rates of test material inclusion was linear (P < 0·001) in all cases except for ORSM and RSM the DE and ME of which, in addition, showed evidence of curvilinearity. Age of pig had no significant effect on dietary AOD and energy values. Linear regression techniques were better than single level assays (particularly at low rates of inclusion) in determining energy values and gave DE values of 42·4, 190, 14·6 and 25·8 MJ/kg dry matter for RSO, FFRS, RSM and ORSM respectively. Oil DE and AOD were not influenced by method of evaluation. The results of this study indicated improved energy values for FFRS and RSM of a triple-low cultivar over published data for high-glucosinolate varieties but not over data for double-low varieties. In addition it appears that AOD is the main factor determining dietary utilization of FFRS and would need to be considered in order to optimize the nutritive value of FFRS in diets for growing pigs.

Two experiments are reported in which a comparison was made of phase feeding and choice feeding as methods of meeting the changing amino acid requirements of growing pigs. In the first experiment, three feeding strategies were used: a system in which a single food (165 g protein per kg food) was offered throughout the growth period; a phase-feeding system, using five different treatments; and three choice-feeding treatments, in which the two diets offered differed only in their protein concentrations. The second experiment consisted of six treatments, three of which constituted a single feeding system, being a high, a medium, (the control) and a low protein food (240, 165 and 100 g protein per kg); there were two phase-feeding treatments, of three and five phases; and one treatment in which a choice was offered of the high and the low protein foods. In both experiments, group data were collected on Landrace × Large White pigs, sexes separate, during the growing period (30 to 90 kg). All pigs were weighed weekly, as was the amount of food consumed in each pen of 10 animals. Phase feeding improved food conversion efficiency (+4·4 g/kg) and caused a decline in both food intake (−45·3 g) and P2 backfat thickness (−0·4 mm) with each increment in the number of phases used. Results of the choice feeding treatments were not statistically significantly different from either the control or the phase feeding treatments. The intake of dietary protein was higher in the choice treatments than in the control (420 v. 370 g in experiment 1 and 345 v. 334 g in experiment 2). Where the two foods on offer differed only in protein content, pigs reduced the proportion of high protein food in the combination chosen by 0037 and 0·059 per week in the two experiments respectively, these linear trends being statistically highly significant. They were less successful in differentiating between the high protein food and maize, the proportion of high protein food chosen decreasing at a statistically significant rate of 0018 per week, but where the maize had not been supplemented with vitamins and minerals there was no significant trend in the way in which the pigs selected their diet, demonstrating the importance of the correct design of the two foods on offer in a choice-feeding programme.

Genotype × environment interaction for age at puberty was evaluated using gilts from the Nebraska Gene Pool population derived from lines selected randomly (RS; no. = 48) or for seven generations for decreased age at puberty (AP; no. = 73). Age at puberty was evaluated in two environments: boar exposure for 15 min daily (BE) or no boar exposure (NBE). Pigs were randomly assigned to treatment and mixed into groups of 20 in pasture lots. Oestrous detection was initiated when the oldest gilt in a pen was 125 days of age. Blood samples were taken weekly and assayed for progesterone. Gilts were considered to have expressed oestrus if they exhibited lordosis or had consecutive weekly blood samples with progesterone values above 6·4 × 109 mol/1 (2 μ, g/l). Gilts were removed from the pen upon confirmation of puberty or at 250 days of age. Two gilts failed to reach puberty by 250 days so this value was assigned as their age at puberty. Least-squares analyses of variance were used to analyse the data. A model including line, sire within line, farrowing group, treatment and line × treatment interaction was fitted to the dependent variables age at puberty and percentage cycling by 185 days. Line and treatment each affected age at puberty (P < 0·05) but did not interact. Least-square mean ages at puberty were 154 (s.e. 4·5), 164 (s.e. 4·7), 164 (s.e. 6·1) and 179 (s.e. 5·9) days for AP-BE, AP-NBE, RS-BE and RS-NBE, respectively. Treatment affected percentage of gilts cycling by 185 days (P < 0·05). Least-square percentages were 91 (s.e. 6·9), 76 (s.e. 71), 84 (s.e. 9·2) and 65 (s.e. 8·9) % for AP-BE, AP-NBE, RS-BE and RS-NBE, respectively. These results indicate that expression of response to selection for decreased age at puberty in the gilt is not dependent on stimuli from the boar.

In two experiments involving ovariectomized Scottish Blackface ewes and steroid replacement therapy, the effects of level of food intake and of body condition on hypothalamic and pituitary sensitivity to progesterone and oestradiol feedback were investigated. In experiment 1, groups of 16 ewes in similar levels of body condition were given dried grass pellets ad libitum (mean dry-matter (DM) intake of 2481 (s.e. 71) g per head per day; group H) or 1105 g DM per head per day (group M). In experiment 2, groups of 16 ewes were fed so that they achieved body condition scores of 2·70 (s.e. 0040; group HBC) or 1·67 (s.e. 0·036; group LBC) prior to the start of the experiment. All ewes were then fed to maintain their respective levels of body condition. In both experiments, all ewes were ovariectomized and intravaginal progesterone pessaries and subcutaneous oestradiol implants were inserted; these were designed to induce circulating concentrations of these steroids within the normal range for intact ewes. Approximately 1 week later, blood samples were collected at 15-min intervals for 10 h, and at 10-min intervals for 8 h before and after pessary withdrawal respectively. All samples were assayed for LH and selected samples were assayed for FSH and progesterone. Gonadotropin profiles were also recorded during 2-h periods following injection (i.v.) of 0·25 μg and 5 μg GnRH. When circulating progesterone concentrations were elevated, due to the presence of a progesterone pessary, there was no effect of level of food intake on mean LH or FSH concentrations or mean LH pulse frequency but H ewes had a higher mean LH pulse amplitude than M ewes (P < 005). When the progesterone pessaries were absent, H ewes had a higher mean LH concentration (P < 001), mean LH pulse frequency (P < 001) and LH pulse amplitude (P < 0·01) than M ewes but mean FSH concentrations were similar in the two groups. Irrespective of the presence or absence of elevated progesterone concentrations, the pituitary responses to GnRH injection were either not significantly different or were lower in H compared with M ewes. In experiment 2, HBC ewes had a higher mean LH concentration (P < 001), LH pulse frequency (P < 0·001), pulse amplitude (P < 0·001) and FSH concentration (P < 001) than LBC ewes, irrespective of circulating progesterone concentrations. The mean heights and ‘areas’ of the LH peak induced by injection of 0·25 μg GnRH were larger in HBC than LBC ewes (P < 005) irrespective of circulating progesterone concentrations. When mean concentrations before injection were taken into account, the corresponding increases in FSH concentration were similar for the two groups. Following injection of 5 u.g GnRH, the differences in peak size were generally not significant. It is concluded that during the follicular phase of the cycle, a low level of intake is associated with increased hypothalamic sensitivity, but not pituitary sensitivity to oestradiol feedback. Body condition affects both hypothalamic and pituitary activity but the relative importance of direct and indirect effects of body condition on the sensitivity of these organs to steroid feedback, remains to be elucidated.

By tracing 14C after an injection of [U-14C] sodium acetate into the caecum of pigs, it was found that proportionately 0·93 of the acetate injected was absorbed. About 0·25 of the administered acetate was recovered in the body 96 h later. It is assumed that the remainder was oxidized during this period and exhaled as carbon dioxide.

The 14C initially predominated in plasma lipids, but it progressively shifted over the 96 h to plasma proteins. In the lipid fraction of blood plasma there was a continuous shift of 14C from triglycerides and free cholesterol to cholesterol esters over the 96-h period of measurement.

In the body most of the 14C was recovered in the fat, liver and intestinal wall. The highest concentration of 14C was in the bile, especially in glycine-conjugated bile acids.

It was shown that the absorbed acetate was a source of energy to the pigs and it was recycled and metabolized into more complex components in the body during 96 h.

A study of the determinants of inter- and intra-litter variation in muscle fibre number was carried out on five litters of Large White piglets. Fresh frozen, whole mid-belly sections of m. semitendinosus were stained to demonstrate acid-stable myosin adenosine triphosphatase activity. From these sections it was possible to identify which fibres had developed as primary and which as secondary fibres. Estimations of total muscle fibre number, total primary fibre number and ratio of secondary fibres to primary fibres were made for each animal. Results demonstrated that primary fibre number varied between litters (P<0·01) and was responsible for the variation in total muscle fibre number (P < 005) between litters since there was no significant variation in secondary: primary ratio. Within-litter differences in total fibre number could be attributed to both the secondary: primary ratio and primary fibre number, in almost equal contributions. However, when only the largest and smallest extremes of the litters were compared, variation in fibre number was due to the significant difference in the secondary: primary ratio (P<0·01). Taken as a whole, the results appear to show that primary fibre number is responsible for all the variation in muscle fibre number between litters, and also makes a significant contribution, with secondary: primary fibre ratio, to the variation present within a litter. The factors responsible for variations in primary and secondary fibre numbers are discussed.

A total of 1754 records for litter traits of 458 D'man ewes sired by more than 45 rams were analysed to estimate repeatability, heritability and genetic and phenotypic correlations. Repeatability estimated by the intraclass correlation method was 0·11, 0·11, 0·12 and 0·15 for litter sizes at birth and at weaning and litter weights at birth and at weaning, respectively. Paternal half-sib estimates of heritability were 0·09 (s.e. 0·06), 0·04 (s.e. 0·05), 0·15 (s.e. 0·07) and 0·08 (s.e. 0·05), respectively. Genetic correlations among the traits were all positive and varied from 0·17 to 0·72, whereas phenotypic correlations ranged from 0·68 to 0·86.

An experiment was conducted to determine the effect of sucrose as an energy source on energy utilization and protein retention by growing pigs. Growing pigs (20 to 50 kg live weight) were restrictively fed (three times maintenance) either a control wheat-based diet (14 MJ digestible energy (DE) per kg), a sucrose-based diet (15 MJ DE per kg) or a wheat-based diet made i so-energetic with the sucrose diet by the addition of oil. Net energy (NE) content of the diet, energy utilization, protein and fat deposition were measured.

Both the sucrose- and the iso-energetic wheat-based diets improved energy utilization and increased NE retention. They also increased fat deposition (P < 0·05) but had no effect on protein deposition (P > 0·05) compared with the wheat-based control. Increased DE utilization in the sucrose-based diet appeared due to (i) lower dietary fibre, (ii) a better balance of amino acids, or possibly due to (iii) increased fat synthesis due to sucrose metabolism. The lack of effect of sucrose on protein deposition appeared due to either (i) an increased amino acid requirement as a result of the higher NE content of the diet or (ii) preferential use of sucrose for fat deposition.