Despite milk being an important product from sheep, there are very few reports of milk production from the complete lactation of dairy sheep. The Improved Awassi in Israel is kept under an intensive system of management with lambs being weaned soon after birth. Records from one such flock were analysed to investigate the suitability of various mathematical functions for describing milk yield from the complete lactations of dairy sheep. This included a consideration of whether the functions could cope with short lactations, a characteristic of dairy sheep, and a limited number of test-day records per lactation.

Four non-linear mathematical functions were investigated (Wood, Morant, Grossman and Pollott), two of which could also be fitted in a linear and a linear weighted form (Wood and Morant). These functions were fitted to weekly data from a ‘typical Awassi lactation curve’, represented by least squares means of daily milk yield from each week of a 40-week lactation derived from an analysis of 25605 test day records. Characteristics of the lactation were calculated from the functions, such as total milk yield, day and level of peak yield and persistency. These functions were also fitted to 1416 individual lactation records of up to 10 test-day records per lactation. The value of the functions was investigated using the residual mean square (RMS) of the fitted curve as an indicator of how well each function described the lactation. Forms of these functions with a reduced number of parameters were also investigated.

The non-linear functions always fitted the data with a lower RMS than their linear equivalent and the weighted form of the linear functions always had a lower RMS than the unweighted form. Of the linear functions, Morant fitted better than Wood. Of the non-linear functions Grossman, Morant and Pollott (additive and multiplicative) fitted the data equally as well but better than Wood. The various functions predicted characteristics of the lactation curve differently; the Wood functions tended to overestimate yield in early lactation and the Morant functions underestimated peak yield.

No function was better suited to short lactations than another. However the three-parameter function of Morant, Pollott multiplicative and Pollott additive were considered to be the most suitable for describing the complete lactation of dairy sheep.

Possible correlated or pleiotropic effects on food intake, growth rate and carcass traits (meat quality and carcass composition traits) were investigated for the prolificacy related Booroola gene (FecB). This gene was introgressed in a Texel sheep population by systematic backcrossing with sires carrying the Booroola gene. In a 3-year experiment 273 spring-born male lambs were offered concentrates ad libitum, after weaning. Lamb carrier effects and dam carrier effects for the FecB gene were estimated, accounting for year, backcross generation, slaughter weight, weaning age, concentrate type, rearing code, age of the dam and litter size as fixed effects. Carriers of the FecB had a higher dressing percentage (+1·15%), longissimus muscle depth (+0·26 mm to +0·7 5mm) and cross-sectional area (+0·98 cm2). However the gene also had a positive effect on subcutaneous fatness (+0·12 points), KKCF (+30 g) and a substantial positive lamb carrier effect on carcass fat tissue content (+3·09%), which reflects a relative increase in fatness of 11·9%. The FecB gene had also very small, significant effects on some meat colour traits. The dam carrier effects of the Booroola gene were negatively associated with food efficiency and with longissimus muscle depth (–1·38 to –2·25 mm). The increased fatness of lambs could imply that for optimal use of FecB, non-carrier terminal sires should be used on heterozygous dams.

This paper describes a two-part study evaluating the use of growth hormone (GH) response to infusions of somatostatin (SRIF) and GH-releasing factor (GRF) as an indicator of genetic merit in dairy cattle. The first part of the study involved the development of a standard test procedure. This was achieved in two sub-trials, the first testing three SRIF doses, 0·6, 3·0 and 15 µg/kg per h, given over an infusion length of 3 h. The second involved infusing 3·0 μg/kg per h SRIF for either 20 min, 1 h or 3 h. The GH response was measured in serum during the infusion and for 2 h following cessation, during which time a rebound response in GH occurred. As a consequence of these trials, a total of 40 British Friesian calves from two lines genetically divergent for yield traits were infused with 3·0 μg/kg per h for a period of 1 h and at the end of the infusion given 0·1 μg/kg GRF. Five hours later, GRF was administered without SRIF. The protocol was repeated on 2 days consecutively. Results suggested that calves of high merit had lower SRIF-induced baselines than their low-line contemporaries. There were no differences in the GH concentrations immediately following GRF administration, either with or without SRIF. The results from this trial, together with previously published work, indicated that calves selected for high milk fat plus milk protein production may be more sensitive to SRIF such that GH is regulated by lower concentrations of SRIF. However, the practical use of GH response to SRIF as an indicator of genetic merit for dairy cattle may be limited because of logistical difficulties in implementation.

Data on lamb survival from birth to weaning of Rambouillet and Finnsheep were analysed with sire-maternal grandsire linear (LM) and threshold (TM) models. Models for the Rambouillet included the effects of year, type of birth, age of dam and sex as fixed effects and sire, maternal grandsire and residual as random effects. For the Finnsheep, fixed effects were year-age of dam combination, type of birth-rearing and sex, and random effects were sire, maternal grandsire and residual. Restricted maximum likelihood (REML) and marginal maximum likelihood (MML) estimates of variance and covariance components were obtained under LM and TM, respectively. The performance of LM and TM was assessed in terms of goodness of fit and predictive ability. Within the Rambouillet breed, heritabilities of additive direct (0·06), additive maternal (0·04) and total effects (0·13) obtained with TM were 2, 1·3 and 1·9 times greater than those obtained with LM, respectively. For the Finnsheep data, estimated heritabilities of direct (0·17), maternal (0·26) and total (0·34) effects using TM were 1·9, 1·4 and 1·6 times greater than the estimates using LM, respectively. Estimated genetic correlation between direct and maternal effects was 0·14 for the Finnsheep with both the LM and TM. Higher genetic correlations between direct and maternal effects (0·44 and 0·62 with LM and TM, respectively) were obtained for the Rambouillet data. Goodness of fit and predictive ability of the models used with the Finnsheep data were better than for the models used with the Rambouillet data, but within breed, no sizable or significant differences were detected between LM and TM. Results indicate that maternal effects were important in lamb survival to weaning; especially in the more prolific Finnsheep breed.

Plasma leptin concentration shows pulsatility and diurnal rhythm in humans. However, there are few reports concerning the 24-h profile of circulating leptin levels in ruminants. Five crossbred ewes were housed in metabolism cages under a 1-h light-dark cycle. The ewes were offered alfalfa hay daily to meet their energy requirement. Blood samples were collected at 15-min intervals for 24 h. Plasma leptin concentrations were determined using a radioimmunoassay and the profile of plasma leptin levels was analysed by the PULSAR algorithmic program for detecting pulse. Plasma leptin concentration changed in a pulsatile fashion. The mean leptin concentration was 2·93 ng/ml. The mean pulse frequency was 4·8 pulses per day and the mean pulse amplitude was 0·67 ng/ml with an average pulse length of 1:13 h. Plasma leptin level was not affected by feeding or lighting cycle. These results indicate that plasma leptin level in sheep shows pulsatility but diurnal rhythm is not exhibited.

This work was designed to investigate whether the profile of non-esterified fatty acids (NEFA) remains constant in response to underfeeding in beef cows, since in other species mobilization of fatty acids (FA) has been shown to be dependent on fatty acid structure. Twenty-one Charolais cows (multiparous, no. = 16 and primiparous, no. = 5) were assigned to one of two diets (control diet (CD; no. = 11) or restricted diet (RD; no. = 10) which, respectively supplied all or 0·7 of energy and protein requirements) after calving. Blood and composite milk samples were collected weekly before the morning meal over a period of 9 weeks. The cows given RD lost significantly more body weight (P < 0·001) and condition score (P < 0·05) than the cows given CD. Plasma NEFA and urea concentrations were also higher in RD than CD during the experiment (P < 0·05 and P < 0·001, respectively). The level of feeding did not affect plasma insulin and glucose concentrations. Milk FA were affected by feeding level. The proportions of short- and medium-chain FA were lower in RD compared with CD (C8: 0, C10: 0, C12: 0 and C14: 0, P < 0·05 and C14: 1 and C16: 0, P < 0·10) while some of the long-chain FA were higher (C17: 0 and C18: 1, P < 0·001 and C17: 1, P < 0·10). Feeding level affected the profile of FA in plasma NEFA. The proportions of C16: 0 and C18: 1 were higher (P < 0·05) in RD cows compared with CD cows while the proportions of C12: 0, C14: 1, C17: 0, C18: 0 and C18: 2 were lower (P < 0·05). There was a feeding level × time interaction in the proportions of different FA in the plasma NEFA and in the total plasma NEFA concentrations. The differences caused by feeding level gradually disappeared with time. The proportion of C18: 1 in plasma NEFA and the total plasma NEFA concentrations were quadratically positively correlated, while the correlation between C18: 2 and total plasma NEFA was negative. In conclusion, when post-partum beef cattle are underfed, plasma NEFA concentrations increase (indicating lipomobilization) and this increase is associated with an increase in the proportion of oleic acid (C18: 1) in plasma NEFA.

In The Netherlands a system has been developed for classification of live pigs based on lean meat proportion. Ultrasonic backfat thickness measurements were taken on 377 live pigs to assess the ability of the developed system for estimation of the lean meat proportion. The measurements were made at the sites mid point (half the distance from the occipital bone to the base of the tail), mid point –2·5 cm and mid point +2·5 cm, 5 cm off the dorsal mid line.

On the same day, these pigs were slaughtered and the lean meat proportions of the carcasses were estimated using the Hennessy Grading Probe (HGP). HGP measurements were taken between the third and fourth from last rib, 6 cm off the dorsal mid line on carcasses (3/4 LR). The day after slaughter, 88 left carcass sides were randomly chosen to be dissected according to a simplified European Union (EU) reference method.

From the fat thicknesses measured, the one at the site mid point on live pigs was the most accurate predictor for the EU lean meat proportion. The use of multiple site measurements, compared with a single site measurement, significantly reduced the residual standard deviation for the estimation of lean meat proportion.

The site mid point on live pigs differed in longitudinal as well as in dorsal-ventral direction from 3/4 LR on carcasses.

The results of this study suggest that multiple backfat thickness measurements on live pigs can be used for prediction of lean meat proportion with sufficient precision for practical use. Therefore, the developed system can serve as a classification system for live pigs.

Nose-rings are often fitted to outdoor commercial pigs to inhibit rooting and reduce consequent pasture damage. During ethological observations of the behaviour of ringed pigs at pasture, subjective impressions suggested that pigs with nose-rings picked up their normal feed, in the form of large nuts (‘roll nuts’), more tentatively than unringed pigs. The observed pigs were gestating sows under one of three treatments: unringed controls (UR), multiple wire ‘clip-rings’ through the upper rim of the snout (CR), or rigid ‘bull-rings’ through the nasal septum (BR). For formal assessment of feeding efficiency eight randomly selected pigs from each condition were timed and video-recorded, in individual feeder stalls, as they ate 20 3-cm long roll-nuts laid out in a standard rectilinear array in a steel-sided tray under each of five conditions: on a hard surface, on the surface of soil, on the surface of turf, embedded in soil, or embedded in turf. All groups took longer to eat up their test meal from the plain surface than from the surfaces of either soil or turf and longer still when the nuts were embedded. In all conditions, ringed pigs took longer to eat their 20 nuts than did controls, with BR > CR > UR (overall mean times, pooling data from the different feeding conditions: 118·6, 69·9 and 56·7 s respectively: P < 0·001). The difference between groups was greatest when nuts were embedded. Also, when nuts were embedded, ringed pigs were more reluctant to root: the latency to their first rooting action was delayed (mean latency across both embedded’ conditions: 29·6, 87·9 and 106·8 s for UR, CR and BR pigs, respectively: P < 0·01 with soil and turf conditions combined) and, even after starting, a smaller proportion of the residual trial time was spent rooting (0·11, 0·27 and 0·62 of the total time by BR, CR and UR pigs respectively; P < 0·001). It is concluded that nose-ringing depresses the efficiency with which pigs feed on solid food, especially if they have to root for it. The threat to welfare inherent in this will be greatest when ringed pigs are in direct competition with unringed pigs.

The effect of two different lactation environments on the development of pig behaviour was examined at weaning and on days 1, 8, 15 and 57 post weaning. Ten focal indoor-bred pigs and 10 focal outdoor-bred pigs were weaned and mixed together into a group of 60 to 80 mixed indoor- and outdoor-bred pigs. There were six replicate groups. Scans of all focal animals were made using time sampling with a 2-min interval, for 3·0 h immediately following weaning and from 06:00 to 11:00 h on days 1, 8, 15 and 57 post weaning. All pens were straw bedded with two different types of feeder system: one seven-space ad libitum hopper and two rooting-action feeders each accommodating up to four piglets at one time.

From weaning to day 1 post weaning, outdoor-bred pigs were observed to feed more than were indoor-bred pigs (115·6 v. 97·0 observations per pen per day, P < 0·05, s.e.d. = 1·18). From days 8 to 57 post weaning, outdoor-bred pigs were observed to root more than were indoor-bred pigs (22·5 v. 14·7 observations per pen per day, P < 0·05, s.e.d. = 1·22). These findings support the hypothesis that the lactation environment has a significant effect on the behaviour of pigs in their subsequent growing environment. However, such effects appear not to lead to differences in carcass weights, rigor following dressing or carcass blemish at slaughter.

The suckling frequency in 34 first-lactation sows and their litters in conventional farrowing pens was observed during a 35-day lactation period from birth to weaning. In order to quantify the ontogenetic development of this behaviour a non-linear regression function was used as a model. The maximum (MAX) of the curve was determined at day 8×5 (31×4 sucklings per 24-h period) and was considered as the biological beginning of the weaning process. The occurrence of MAX was shifted towards an earlier time by about 6 days earlier from small (4 to 7 piglets per litter) to large litters (11 to 14 piglets per litter). However, no significant influence of the litter size on the development of the daily suckling frequency and the average individual piglet weight was found. Nevertheless, there was a tendency for an inverse relationship between the litter size and the piglet weight during lactation and, further, for an increased daily suckling frequency during early lactation and a lower frequency during late lactation in larger litters. As a compensation for the decreasing suckling frequency piglets markedly increased their creep food consumption in the last week of the suckling period (day 28 to 35). The results indicate that the suckling behaviour in domestic pigs reflects sow-piglet relationships which are consistent with a theoretically predicted model of weaning conflict. Hence, it is suggested that modelling the suckling behaviour using well defined and comparable measures may be a suitable approach for the evaluation of the weaning process both in terms of sociobiology (e.g. weaning conflict, parent-offspring conflict) and of farm animal science (e.g. performance, housing conditions).

A 2 ✕ 2 ✕ 3 factorial arrangement of treatments used 522 boars to examine the main effects and interactions of season (spring v. summer), number of pigs per pen with a single-space feeder (13 or 16 pigs per pen with a singlespace feeder; 0·78 m2 per pig v. 0·65 m2 floor space per pig) and nutrient density of the diet (12·5 g lysine per kg and 14·0 MJ digestible energy (DE) per kg from 38 kg to slaughter (high), 12·5 g lysine per kg and 14·0 MJ DE per kg during the growing phase and 10·0 g lysine per kg and 13·6 MJ DE per kg during the finishing phase (split) and 10 g lysine per kg and 13·6 MJ DE per kg from 38 kg to slaughter (low)) on the performance and carcass characteristics of growing and finishing pigs. The smaller group of pigs had a higher food intake during the grower-finisher period than the high stocked pigs (P < 0·01). The pigs had a higher lysine intake in the spring than in the summer months (P < 0·05). Differences in daily food intake for the smaller group of pigs were reflected in increased growth rate during the grower-finisher period (P < 0·001). However these pigs had a poorer food conversion ratio during the grower-finisher period (P < 0·05) than the bigger group of pigs. The pigs offered the high specification and split-fed diets had better growth rate (P < 0·05) than the pigs given the low specification diet during the grower and finisher period. There was a significant two-way interaction in growth rate between dietary treatment and season during the finisher period (P < 0·01). The pigs given the high specification diet had a better growth rate (P < 0·001) than the pigs given the low diets during the summer months. However, the high specification diet depressed growth rate during the spring months (P < 0·05). The pigs had a lower backfat and higher lean meat in the summer than in the spring months (P < 0·05).

The effects of different dietary essential fatty acids on piglet tissue composition at birth and performance until 7 days post weaning were investigated by offering the sow diets containing (17·5 g oil per kg diet) either maize oil (MO) as a control treatment, tuna oil (TO) as a source of long chain n-3 polyunsaturated fatty acids, particularly 22:6 n-3, or a mixture of maize and linseed oils (LO) which supplied the same amount of n-3 acids as TO but in the form of 18:3 n-3. Ten sows were allocated to each treatment which was offered throughout pregnancy and lactation. Compared with MO, offering TO increased sow plasma and subcutaneous adipose tissue 22: 6 n-3 proportions whereas LO increased 18: 3 n-3 and, to a much lesser extent than TO, 22: 6 n-3. Offering TO to the sow increased the proportions of 20: 5 n-3 and 22: 6 n-3 in piglet brain and liver at birth and decreased the n-6 acids, 20: 4, 22: 4 and 22: 5. LO only increased piglet liver 20: 5 n-3 proportions but to a lesser extent than TO; however, LO also decreased the proportions of 20: 4, 22: 4 and 22: 5 n-6 in piglet tissues. Offering the pregnant sow dietary 18: 3 n-3 therefore increased deposition of 22: 6 n-3 in foetal piglet tissues to a much lesser extent than tuna oil and so it is necessary to offer the sow pre-formed 22: 6 n-3 in order to achieve maximum foetal 22: 6 n-3 deposition. By experimentally allocating piglets at birth, effects of sow nutrition during pregnancy and lactation were separated. Piglets sucking MO or TO sows were heavier than piglets sucking LO sows 7 days post weaning.

Growing pigs fitted with post-valve T-caecum cannulas were used in a change-over design experiment (5 ✕ 5) to determine the ileal and total tract apparent digestibility and hindgut disappearance of dietary components and energy in a cassava root and soya-bean meal-based diet with an inclusion rate at 0·15 of the dry matter (DM) of either cassava leaf meal, ensiled cassava leaves, groundnut foliage, or leucaena leaves.

The ileal and total tract apparent digestibilities of organic matter (OM), crude protein, nitrogen-free extractives and energy of the diets were significantly reduced with inclusion of the foliages (P < 0·05). The digestibility of neutral-detergent fibre (NDF) and crude fibre was improved when groundnut foliage was included in the diet (P < 0·05).

The dietary content of NDF was a better predictor of the total tract digestibility of OM (R2 = 0·87) and energy (R2 = 0·90) than the content of crude fibre (R2 = 0·68 for OM digestibility and R2 = 0·72 for energy digestibility).

The disappearance of OM and energy in the hindgut of the foliage diets was 0·36 (s.d. 0·02) and 0·31 (s.d. 0·02), respectively, which was significantly lower (P < 0·05) than on the control diet (0·48 and 0·46, respectively).

Ileal as well as total tract digestibilities of OM in groundnut foliage were higher than for the other leaf products (ileal, 0·55 and 0·42; and total tract, 0·64 and 0·55, respectively). For foliage products of cassava and leucaena an average digestible energy value of 11·4 MJ/kg DM was calculated, whereas for groundnut foliage the corresponding value was 10·9 MJ/kg DM.

It was concluded that, inclusion of foliages in pig diets will reduce the dietary energy content, due to a high fibre content. However, under tropical conditions foliage at low levels of inclusion may be useful in low fibre diets for growing pigs to improve the dietary protein supply.

The aim of this work was to study the effect of the contemporary administration, in separate troughs, of two diets differing in digestible energy (DE) and crude fibre (CF) content, on the performance of lactating rabbit does. At the first mating (130 days of age and 3·8 kg), 54 nulliparous rabbit does (New Zealand) were assigned to the control group offered a conventional lactation diet (diet C: 188 g crude protein (CP) per kg dry matter (DM) and 11·43 MJ DE per kg DM); whilst another 54 rabbit does were offered at the same time, in separate troughs, diet E (203 g CP per kg DM and 12·66 MJ DE per kg DM) and diet F (178 g CP per kg DM and 10·73 MJ DE per kg DM). The contemporary administration of two diets did not significantly affect food intake. However, the rabbit does in group F + E, due to the greater consumption of diet E (approx. 0·76 of the total), consumed more digestible protein (DP) both during the first (14·40 v. 13·44 g/day per kg M0·75 and 80·1 v. 73·5 g/day per kg M0·75, respectively for days 1 to 21 and days 22 to 35) and subsequent lactations (15·37 v. 14·35 g/day per kg M0·75 and 82·6 v. 76·5 g/day per kg M0·75 respectively for days 1 to 21 and days 22 to 35). Also the DE intake was higher for the rabbit does of group F + E during the 22- to 35-day period (6708 v. 6361 kJ/day and 6918 v. 6620 kJ/day respectively for primiparous and multiparous). The improved performance of rabbit does given two different diets does not justify the extra time involved in such a technique, as the intake of diet E was constant during the experimental period and was not influenced by the different energy requirements during lactation.

Fifteen sexually mature rams, five each of Barki, Awassi (I, imported from Syria) and Awassi (LB, locally born in Egypt) were used in this study. Semen was collected monthly from rams for a period of 12 months to study semen characteristics. In addition, blood samples were collected from rams during the four seasons of the year to determine serum triiodothyronine (T3) and testosterone hormones. Results showed that Barki and Awassi (I and LB) rams are continuous breeders as they show sexual desire and produce semen all the year round. However, monthly variations in semen quality were detected. Relative testes volume, ejaculate volume, sperm concentration, total sperm output, sperm motility, percentage of live sperm and serum testosterone level were higher during summer months than at other months of the year. Serum T3 was significantly higher in winter and spring than that observed in summer and autumn. In addition, percentages of dead spermatozoa were higher in winter and early spring than in autumn and summer. Furthermore, summer months showed moderate percentages of abnormal spermatozoa and spermatozoa without intact acrosomes. These results indicate that ram semen quality in the subtropics may be better during summer months than during other months of the year.

Fifteen sexually mature rams, five each of Barki, Awassi (I, imported from Syria) and Awassi (LB, locally born in Egypt) were used to study the biochemical and enzymatic properties of seminal plasma for 12 months. The biochemical analyses of ram seminal plasma indicated that the highest values of total protein, albumin (A), globulin (G), fructose, total lipids and cholesterol were recorded in late summer and early autumn, while A/G and AST/ALT ratios exhibited the lowest values. Lowest values of the same parameters were recorded in winter and late spring, while ratios of A/G and aspartate amino transferase (AST)/alanine amino transferase (ALT) showed the highest values in spring. AST enzyme activity showed highest activity in winter and summer and lowest activity in autumn, while ALT exhibited the highest activity in winter and the lowest activity in spring. The relationships between these parameters and semen quality are discussed.

Sixty-four Azawak male calves were used to study the effect of nocturnal grazing (NG) and supplementation (S) in the dry season on forage and water intake, faecal output, eating time and weight changes of cattle in the Sahel. Treatments were factorial combinations of four levels of NG (0, 2, 4 and 6 h/day) and two levels of S (0 and 608 g dry matter (DM) per animal per day). All animals were allowed to graze 10 h during the day and were weighed every 2 weeks during the 70-day experimental period. Eight oesophageally fistulated steers were used in a cross-over design to sample the diet (forage) selected during the day and at night by supplemented and non-supplemented animals. Extrusa crude protein and in vitro organic matter digestibility were not influenced by supplementation (P > 0·05). Time spent eating during the day or at night were not affected by supplementation but total eating time increased by 39·4 (s.e. 2·1) min/h of NG. Forage intake increased with increase in NG, while total food intake (forage + supplement) increased with supplementation (82·4 v. 92·1 (s.e. 2·4) g DM per kg M0·75 per day). The supplemented animals also drank more water than the non-supplemented (26·2 v. 24·8 l per animal per day). Average live-weight change (LWC) increased by 24·4 (s.e. 8·7) and 9·3 (s.e. 6·2) g/h of NG in non-supplemented and supplemented animals, respectively. Supplementation improved LWC (–107 v. 99 g/day, s.e. 14, P < 0·05). Night grazing improves dry season performance and its effect decreases when cattle are supplemented.

Switchgrass (Panicum virgatum cv. Alamo) standing hay was cut and offered to 16 rams. The rams were randomly allocated to one of the following levels of feeding: 0·5; 1·0; 1·5 and 2·0 ✕ maintenance. The forage was offered twice a day to the animals; no supplements were given. The animals were individually penned and fitted with faeces collection bags. After 8 days for adaptation, in vivo dry matter apparent digestibility (DMD) and proportion of blades and stem + sheath of the consumed forage were measured for 8 days. DMD varied from 0·310 (0·5) to 0·391 (2·0) and the proportion of the blade fraction in the diet from 0·44 (0·5) to 0·78 (2·0). The short-term intake rate (STIR) of rams was measured for 5 days. The animals were divided randomly into four blocks, with one ram of each treatment in each block. After 4 h without food and 15 min intervals between blocks, the amount of forage consumed during 4 min of active eating was measured. The amounts consumed were (in g DM per min per 50 kg live weight): 0·5: 8·2; 1·0: 7·8; 1·5: 5·3 and 2·0: 5·3 (0·5 = 1·0 > 1·5 = 2·0; P < 0·01). The results show that offer rate of P. virgatum affects the blade: stem + sheath ratio of intake and, therefore, the nutritive value of the diet. Level of feeding before measuring STIR influences the amount of forage consumed during the test.

Twelve dairy cows in early lactation were offered low (L; 215 g/kg) or high (H; 449 g/kg) dry matter(DM) content silages, prepared using material from the same sward. In addition, all animals received 9 kg/day, of supplements based on barley (B), sugar-beet pulp (SB) or a 50: 50 mixture of the two (B: SB), in two equal portions at 07:30 and 14:30 h. The six treatments were offered in an incomplete Latin square design. Mean intakes of H (14·4 kg DM per day) were significantly higher than intakes observed for L (10·0 kg DM per day) (P < 0·001). Within silage type, highest intakes were observed for cows receiving the SB supplement (P < 0·01). Higher intakes of H were reflected in higher total milk yield (P < 0·05) as well as fat (P < 0·05) and protein (P < 0·01) yield. Milk protein concentration was greater for animals receiving silage H (P < 0·001), with lower values being observed for animals consuming SB (P < 0·05), within silage type. Time spent eating, duration and number of meals were similar for either silage and the higher intakes of H silage reflected greater intake rates (g DM per min) (P < 0·001) resulting in larger meal sizes (P < 0·001). All chewing indices (time spent eating silage, ruminating and total time chewing per kg DM ingested) were greater for the L silage (P < 0·001). It is concluded that the benefits in forage intake with higher DM grass silages, for high yielding dairy cows, are associated with consequential benefits in milk yield and milk protein content. The most likely explanation for the greater intakes is a faster particle breakdown in the rumen allowing larger meal sizes before animals became constrained. The higher intakes of silage when animals consumed the SB supplement may be due to a slower rate of fermentation of the supplement, which was more closely matched to that of silage. Although not significant there was a tendency for differences in silage intake between animals receiving B compared with SB supplements to be greater for animals receiving the H silage suggesting that supplementation strategies to ensure optimal forage utilization may differ for silages of differing DM content.

In a 12-week trial, 12 Alpine and 12 Saanen dairy goats in mid lactation were housed in individual stalls for behavioural, digestive and metabolic studies. Eight of them were fitted with ruminal cannulae. They were offered ad libitum four complete diets CR, CS, FR and FS (450 g dry matter (DM) per kg) in a 4 ✕ 4 Latin-square design. Forage: concentrate ratio was either low (C = 30: 70) or high (F = 55: 45) and starch source either rapidly (R, barley) or slowly (S, maize) degraded in the rumen.

Diet preferences were tested. DM intake, chewing activities and performance were determined. Ruminal pH and ammonia-nitrogen (NH3-N) concentrations were measured. Goats were also challenged intravenously with glucose (0·2 g/kg live weight) to assess glucose homeostasis.

CR was the most preferred diet. The level of intake differed according to the type of starch (2·2 kg DM per day of CR and FR and 2·0 kg DM per day of CS and FS). FS lowered ruminal pH significantly less than the other diets after the meal. Ruminal NH3-N concentrations were lower in goats given CR and CS than FR and FS. Goats’ resistance to a glucose challenge was not different among diets. Milk yield was 2·00, 1·85, 2·01, 1·85 kg/day on CR, CS, FR and FS respectively. Milk fat content decreased significantly with diets rich in concentrate (24 v. 26 g/kg milk) but milk protein content varied only a little.

In conclusion it appears that (1) higher forage: concentrate ratios were less preferred and decreased ruminal acidity but increased daily chewing, ruminal NH3-N and milk fat content; and (2) rapidly degraded starch increased intake, ruminal acidity and milk yield.