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7 - In situ analysis of microRNA expression during vertebrate development

from II - MicroRNA functions and RNAi-mediated pathways

Published online by Cambridge University Press:  22 August 2009

By
Diana K. Darnell ,
Stacey Stanislaw ,
Simran Kaur ,
Tatiana A. Yatskievych ,
Sean Davey ,
Jay H. Konieczka  and
Parker B. Antin
Edited by
Krishnarao Appasani
Foreword by
Sidney Altman  and
Victor R. Ambros
Diana K. Darnell
Affiliation:
Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA
Stacey Stanislaw
Affiliation:
Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA
Simran Kaur
Affiliation:
Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA
Tatiana A. Yatskievych
Affiliation:
Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA
Sean Davey
Affiliation:
Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA
Jay H. Konieczka
Affiliation:
Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA
Parker B. Antin
Affiliation:
Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA
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Summary

Introduction

A widespread class of non-coding, regulatory RNAs has been recently characterized. Because of their short length (21–22 nucleotides) they are called microRNA or miRNA. These miRNAs have been identified in diverse organisms (prokaryote, eukaryote, vertebrates, invertebrates, plants, fungi) and in viruses (Tuschl et al., 1999; Elbashir et al., 2001; Griffiths-Jones, 2004; Berezikov and Plasterk, 2005; Griffiths-Jones et al., 2006). Their apparently ancient function is to regulate specific protein concentration by inhibiting the first step of translation or by inducing specific mRNA degradation by 3′ UTR binding (He and Hannon, 2004; Pillai, 2005; Valencia-Sanchez et al., 2006). Both molecular and bioinformatics tools have been used to identify candidate miRNAs and their target mRNAs. Based on the numbers generated in these studies, it is estimated that vertebrate genomes may contain hundreds of miRNA genes that may regulate stability or translation of approximately one quarter of all mRNAs (Bentwich et al., 2005; Berezikov and Plasterk, 2005; Legendre et al., 2005; Xie et al., 2005).

Disruption of miRNA function often produces aberrations of important processes including organogenesis, and cell diversification, proliferation, and survival (Reinhart et al., 2000; Brennecke et al., 2003; Dostie et al., 2003; Ambros, 2004; Calin et al., 2004; Alvarez-Garcia and Miska, 2005; Giraldez et al., 2005). The miRNA function has also been implicated in regulating stem cell renewal and the onset of certain cancers (Hatfield et al., 2005, Lu et al., 2005). Therefore, miRNAs regulate important processes in animal development, physiology and disease.

Type
Chapter
Information
MicroRNAs
From Basic Science to Disease Biology
 , pp. 102 - 114
Publisher: Cambridge University Press
Print publication year: 2007

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References

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  • In situ analysis of microRNA expression during vertebrate development
    • By Diana K. Darnell, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Stacey Stanislaw, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Simran Kaur, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Tatiana A. Yatskievych, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Sean Davey, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Jay H. Konieczka, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Parker B. Antin, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA
  • Edited by Krishnarao Appasani
  • Foreword by Sidney Altman, Victor R. Ambros
  • Book: MicroRNAs
  • Online publication: 22 August 2009
  • Chapter DOI: https://doi.org/10.1017/CBO9780511541766.010
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  • In situ analysis of microRNA expression during vertebrate development
    • By Diana K. Darnell, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Stacey Stanislaw, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Simran Kaur, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Tatiana A. Yatskievych, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Sean Davey, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Jay H. Konieczka, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Parker B. Antin, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA
  • Edited by Krishnarao Appasani
  • Foreword by Sidney Altman, Victor R. Ambros
  • Book: MicroRNAs
  • Online publication: 22 August 2009
  • Chapter DOI: https://doi.org/10.1017/CBO9780511541766.010
Available formats
×

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To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

  • In situ analysis of microRNA expression during vertebrate development
    • By Diana K. Darnell, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Stacey Stanislaw, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Simran Kaur, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Tatiana A. Yatskievych, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Sean Davey, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Jay H. Konieczka, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA, Parker B. Antin, Department of Cell Biology and Anatomy University of Arizona PO Box 245044 Life Sciences North 462 1501 N. Campbell Avenue Tucson, AZ 85724-5044 USA
  • Edited by Krishnarao Appasani
  • Foreword by Sidney Altman, Victor R. Ambros
  • Book: MicroRNAs
  • Online publication: 22 August 2009
  • Chapter DOI: https://doi.org/10.1017/CBO9780511541766.010
Available formats
×