Nineteenth-century Cherokee printers were media theorists who made political arguments through the materiality of Christian tracts. This article turns to the tract Poor Sarah as an illuminating example, especially because Cherokees published it in two editions in 1833 and 1843, affording a comparative analysis from before and after the tribe's forced removal from Cherokee Nation to Indian Territory. The material qualities of the two editions were strikingly different. Before removal, Cherokee printers emulated Anglo-Protestant prototypes in terms of dimensions, layout, and typography. The goal was to increase the likelihood of staying on their lands by winning white patrons and nudging Cherokee readers to see themselves as acculturated reading subjects. After removal, Cherokee printers rethought acculturation as a strategy. They redesigned Poor Sarah to turn away from white audiences and instead address the Cherokee community and its needs. Attention to the evolving materialities of Poor Sarah reveals Cherokee Christian printing as a key site of Indigenous media theory and part of Cherokee Nation's repertory of political action in response to US imperialism and settler colonialism.