Field studies were conducted over 3 yr to evaluate rhizome johnsongrass control with asulam applied POST at 3.7 kg ai/ha 3 d before fertilization (DBF) or 0, 3, 7, 10, or 14 d after fertilization (DAF). Liquid fertilizer (18-6-12) was applied with injector knives at 0, 112, or 224 kg N/ha 5 cm on each side of a 60-cm line of rhizome johnsongrass present on 1.8-m-wide conventional sugarcane beds. Johnsongrass response to timing of asulam application after fertilization varied among years but was not affected by fertilizer rate. Johnsongrass control with asulam applied 3 DBF was greater than applications made 0, 3, or 7 DAF. With one exception, differences in johnsongrass control following fertilization and asulam application times were also reflected in the biomass of treated johnsongrass and its regrowth harvested 6 and 10 wk after asulam application, respectively. Reduced johnsongrass control associated with asulam application following fertilization was related to stress from root/rhizome injury during fertilizer placement rather than the quantity of fertilizer applied.