Herbicide treatments (4:1 ratio of 2,4-D amine:picloram) at 0.7 and 1.4 kg ae/ha at early postemergence (10- to 15-cm horsenettle height), midpostemergence (early flower), and late postemergence (fruit initiation) applied both early and late in the growing season provided >80% horsenettle control. Horsenettle density at season's end in all treated plots was less than 0.25 stems/m2, whereas untreated plots contained about 5 stems/m2. Horsenettle control the next spring was between 47 and 66% for all rates and application timings, and horsenettle density in treated plots was less than 3 stems/m2 as opposed to about 6 stems/m2 in the untreated plots. Clover drilled into the treated area the year after herbicide application was injured, indicating clover establishment the season after application of this package mixture would be difficult.