The chronology of the Mesolithic, Neolithic, Aeneolithic, and Bronze Age sites in the Trans-Urals (Russia) was studied, and a framework for previously established cultural complexes is suggested based on 153 radiocarbon dates. In this new chronological model, the Mesolithic is dated to ~10,000–6500 cal BC; the Neolithic complexes exist at ~6500–3800 cal BC; the Aeneolithic is dated to ~4300–2800 cal BC; the Early Bronze Age spans ~2500–2100 cal BC; and the Late Bronze Age can be preliminary assigned to ~1600–1100 cal BC.