Introduction.Barberry (Berberis aristata DC.), a small fruit,is found growing in the wild in the temperate region of northernPakistan. Materials and methods. Three different locations ofAzad Jammu and Kashmir, i.e., Topa, Banjosa andNeriyan Sharif, were explored; thirty accessions of barberry wereidentified and characterized on the basis of their growth habit,flowering, fruiting and chemical composition of fruits. Results.The accessions exhibited high diversity in their phenotypical/morphologicaltraits such as growth habit, intensity and time of flowering, fruitsetting, ripening time, productivity, and fruit shape and color.The location had a great impact on quantitative parameters. Accessionscollected from Neriyan Sharif had significantly greater plant height,higher number of branches per plant, extended flowering duration(days), took more time to maturity (days) and had high fruit setting (%),while those from Topa had large-sized leaves and fruits with greateraverage fruit weight. The nutritional composition of fruits indicatedthat the accessions from Topa had higher carbohydrate, ash, total solublesolids and Mn contents, with significantly higher juice pH, andlower acidity; while the accessions from Neriyan Sharif had significantlyhigher moisture, protein, fat, fiber, K, Ca, Na, Fe, Cu, Pb andCr contents and were highly acidic. The accessions collected fromBanjosa were almost intermediate for the quantitative characteristicsstudied. Conclusions. The results suggested that notonly the genotype but its growing location as well are the mainfactors that determine the growing habit, productivity and nutritionalcomposition of barberry fruits, and this ultimately provides importantinformation on how to make the best use of them.