Scarification in sulfuric acid consistently increased germination of Opuntia edwardsii sp. nov, O. discata Griffiths, and O. lindheimeri Engelm. ♯ OPULI seeds over that of untreated seeds. Optimum constant temperatures for germination were generally 25 to 35 C and germination was not enhanced by alternating temperatures. There was a trend for increased germination following leaching in water for 12 h which suggested the presence of chemical germination inhibitors. Seeds passed through the digestive tracts of cattle exhibited average germination percentages that were 1.5 times greater than seeds removed from ripe fruits.