Enhanced dissipation of atrazine has been documented in many parts of the world where the herbicide has been extensively used. Atrazine is widely used in corn in Illinois, but enhanced dissipation in the field has not been documented. In this study, the dissipation of atrazine was measured in three fields. Two of the fields (URB-1 and URB-2) had not been treated with atrazine for 3 and 4 yr, respectively, whereas the third field (ORR-1) had received annual applications of atrazine for 9 yr. A laboratory assay conducted on soil collected from these fields prior to atrazine application indicated that soil from ORR-1 had enhanced atrazine dissipation (half life [DT50] 1.7 d). Soil from fields URB-1 and URB-2 dissipated atrazine much slower (DT50 was 13 and 11d, respectively). In the field, the rates of atrazine dissipation were similar to those measured in the laboratory. The DT50s were 2.4, 8.4, and 12.6 d for ORR-1, URB-2, and URB-1, respectively. Testing the soils collected from the fields at the last sampling time for atrazine dissipation showed that all of them had enhanced atrazine dissipation, suggesting that the soil microbial populations had adapted to the presence of atrazine in the field.