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Numerous Fasciola plasminogen-binding proteins may underlie blood-brain barrier leakage and explain neurological disorder complexity and heterogeneity in the acute and chronic phases of human fascioliasis

Published online by Cambridge University Press:  24 September 2018

J. González-Miguel
Affiliation:
Laboratorio de Parasitología, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA-CSIC), Cordel de Merinas 40-52, 37008 Salamanca, Spain
M. A. Valero
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
M. Reguera-Gomez
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
C. Mas-Bargues
Affiliation:
Departamento de Fisiología, Facultad de Medicina, Universidad de Valencia, Av. Blasco Ibáñez No. 15, 46010 Valencia, Spain
M. D. Bargues
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
F. Simón
Affiliation:
Área de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Av. Licenciado Méndez Nieto s/n, 37007 Salamanca, Spain
S. Mas-Coma*
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
*
Author for correspondence: Santiago Mas-Coma, E-mail: [email protected]

Abstract

Human fascioliasis is a worldwide, pathogenic food-borne trematodiasis. Impressive clinical pictures comprising puzzling polymorphisms, manifestation multifocality, disease evolution changes, sequelae and mortality, have been reported in patients presenting with neurological, meningeal, neuropsychic and ocular disorders caused at distance by flukes infecting the liver. Proteomic and mass spectrometry analyses of the Fasciola hepatica excretome/secretome identified numerous, several new, plasminogen-binding proteins enhancing plasmin generation. This may underlie blood-brain barrier leakage whether by many simultaneously migrating, small-sized juvenile flukes in the acute phase, or by breakage of encapsulating formations triggered by single worm tracks in the chronic phase. Blood-brain barrier leakages may subsequently occur due to a fibrinolytic system-dependent mechanism involving plasmin-dependent generation of the proinflammatory peptide bradykinin and activation of bradykinin B2 receptors, after different plasminogen-binding protein agglomeration waves. Interactions between diverse parasitic situations and non-imbalancing fibrinolysis system alterations are for the first time proposed that explain the complexity, heterogeneity and timely variations of neurological disorders. Additionally, inflammation and dilation of blood vessels may be due to contact system–dependent generation bradykinin. This baseline allows for search of indicators to detect neurological risk in fascioliasis patients and experimental work on antifibrinolytic treatments or B2 receptor antagonists for preventing blood-brain barrier leakage.

Type
Research Article
Creative Commons
Creative Common License - CCCreative Common License - BY
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright
Copyright © Cambridge University Press 2018

Introduction

Fascioliasis is a worldwide food-borne trematodiasis caused by two Fasciola species transmitted by freshwater lymnaeid snails: Fasciola hepatica in Europe, Africa, Asia, the Americas and Oceania, and Fasciola gigantica in parts of Africa and Asia (Mas-Coma et al., Reference Mas-Coma, Valero and Bargues2009). This disease is well known in the veterinary field due to the economic losses it causes in animal husbandry including livestock morbidity and mortality (Torgerson and Claxton, Reference Torgerson, Claxton and Dalton1999). A new serious public health scenario progressively appeared from the 1990s (Chen and Mott, Reference Chen and Mott1990), with the description of many human endemic areas and non-stop increasing number of case reports (Mas-Coma et al., Reference Mas-Coma, Bargues and Valero2014a). This led to demonstrate that it is the vector-borne parasitic disease with the widest latitudinal, longitudinal and altitudinal distribution known and to reach a global estimation of 17 million people infected (Mas-Coma, Reference Mas-Coma2005).

This new scenario emphasizes the importance of the disease chronic phase, hitherto considered secondary when compared with the acute phase. Indeed, almost all (if not all) subjects diagnosed in human endemic areas are in the chronic stage (Mas-Coma et al., Reference Mas-Coma, Bargues and Valero2014a), whereas in developed countries many or most patients are diagnosed in the acute phase (Arjona et al., Reference Arjona, Riancho, Aguado, Salesa and Gonzalez-Macías1995). Efforts to go deep in the advanced chronic phase demonstrated its implications in pathogenicity (Valero et al., Reference Valero, Santana, Morales, Hernandez and Mas-Coma2003, Reference Valero, Navarro, Garcia-Bodelon, Marcilla, Morales, Garcia, Hernandez and Mas-Coma2006, Reference Valero, Girones, Garcia-Bodelon, Periago, Chico-Calero, Khoubbane, Fresno and Mas-Coma2008, Reference Valero, Bargues, Khoubbane, Artigas, Quesada, Berinde, Ubeira, Mezo, Hernandez, Agramunt and Mas-Coma2016), immunogenicity (Girones et al., Reference Girones, Valero, Garcia-Bodelon, Chico-Calero, Punzon, Fresno and Mas-Coma2007) and in the reinfections during this chronic period (Valero et al., Reference Valero, Perez-Crerspo, Chillon-Marinas, Khoubbane, Quesada, Reguera-Gomez, Mas.-Coma, Frersno and Girones2017).

A recent analysis has proved that a very wide range of neurological, meningeal, neuropsychic and ocular disorders caused at distance by flukes infecting the liver may be relatively frequent but underestimated due to misdiagnosis, mainly in low-income regions. The impressive clinical pictures comprise puzzling polymorphisms, disconcerting multifocality of the manifestations, changes along the disease evolution in a patient and differences between the clinical pictures shown by different patients. In these patients, moreover, post-treatment sequelae and mortality should be emphasized (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b). No mechanism able to explain the triggering of neurological, meningeal and/or ophthalmic disorders at distance by flukes present in the liver or neighbouring tissues has been so far proposed.

Although the molecular and biochemical mechanisms that occur between the host and the parasite are not fully understood, cathepsins and other proteases present in excretory/secretory extracts of F. hepatica have been related to tissue penetration (Robinson et al., Reference Robinson, Menon, Donnelly, Dalton and Ranganathan2009; Meemon and Sobhon, Reference Meemon and Sobhon2015). Migration through host tissues and establishment have also been linked with the interaction between different groups of parasites and the final enzyme of the fibrinolytic system, the protease of serines, plasmin (González-Miguel et al., Reference González-Miguel, Siles-Lucas, Kartashev, Morchón and Simón2016).

The fibrinolytic system comprises the mechanisms responsible for lysing the fibrin clot once formed and maintain, together with the coagulation cascade and platelets, a correct hemostatic balance in the circulatory system (Castellino and Ploplis, Reference Castellino and Ploplis2005; Chapin and Hajjar, Reference Chapin and Hajjar2015). From a molecular point of view, fibrinolysis is mediated by the conversion of plasminogen to its proteolytically active form, plasmin. This process is strictly regulated by binding of plasminogen to receptors through its five kringle domains, which have an affinity for lysine residues and for their activators, namely tissue plasminogen activator (tPA) and urokinase (uPA) (Cesarman-Maus and Hajjar, Reference Cesarman-Maus and Hajjar2005). Plasmin as an active protease is capable of efficiently degrading the formed clot, but also different components of the extracellular matrix and connective tissue. This allows to relate the recruitment of this enzyme by blood and/or tissue-parasites with their dissemination, establishment and survival in host tissues (Figuera et al., Reference Figuera, Gómez-Arreaza and Avilán2013; González-Miguel et al., Reference González-Miguel, Siles-Lucas, Kartashev, Morchón and Simón2016).

From the point of view of fascioliasis, the interest of this fibrinolytic system and the tPA mainly, but not only, relies on the multifunctional roles of plasmin in inflammation (Li, Reference Li2005; Syrovets et al., Reference Syrovets, Lunov and Simmet2012; Lin and Hu, Reference Lin and Hu2014) including central nervous system affection (Gur-Wahnon et al., Reference Gur-Wahnon, Mizrachi, Maaravi-Pinto, Lourbopoulos, Grigoriadis, Higazi and Brenner2013) and blood-brain barrier permeability (Yepes et al., Reference Yepes, Sandkvist, Moore, Bugge, Strickland and Lawrence2003; Michalski et al., Reference Michalski, Hobohm, Weise, Pelz, Heindl, Kamprad, Kacza and Härtig2012; Niego et al., Reference Niego, Freeman, Puschmann, Turnley and Medcalf2012; Jin et al., Reference Jin, Liu and Liu2014; Niego and Medcalf, Reference Niego and Medcalf2014; Marcos-Contreras et al., Reference Marcos-Contreras, Martinez de Lizarrondo, Bardou, Orset, Pruvost, Anfray, Frigout, Hommet, Lebouvier, Montaner, Denis Vivien and Gauberti2016; Suzuki et al., Reference Suzuki, Nagai and Umemura2016) including neurological effects (Fanne et al., Reference Fanne, Nassar, Yarovoi, Rayan, Lamensdorf, Karakoveski, Vadim, Jammal, Cines and Higazi2010; Dong et al., Reference Dong, Hu, Shen, Pan, Wei, Liu, Ren, Liang, Wang, Zhao and Xie2016).

The objective of the present work is to study the interaction between an excretory/secretory extract of F. hepatica worms and the fibrinolytic system of its host by analysing their pro-fibrinolytic potential and to identify by proteomic techniques the antigens responsible for this interaction. The baseline furnished by the results obtained is analysed within the context of the complexity and heterogeneity of the clinical pictures shown by fascioliasis patients presenting with the aforementioned disorders. A proposal is finally exposed which for the first time allows to explain the different clinical situations reported in such fascioliasis patients.

Materials and methods

Materials

A F. hepatica isolate and lymnaeid snail vectors from a human fascioliasis endemic area were used. Metacercariae were obtained from experimentally infected Galba truncatula snails at the Department of Parasitology, University of Valencia, stored in freshwater at 4° C until required and administered to male rats after checking viability by use of the refractile appearance of the excretory granules as a criterion. Galba truncatula that shed the cercariae that gave rise to the metacercariae were from a laboratory-reared strain (in Heraeus-Vötsch HPS 1500 and HPS 500 climatic chambers; experimental conditions: temperature, 20° C; photoperiod, 12 h of light and 12 h of darkness; relative humidity, 90%). These snails were, in turn, infected by one miracidium (Mas-Coma et al., Reference Mas-Coma, Funatsu and Bargues2001). The Wistar rat was used as an animal model because of its characteristics similar to the human host from the points of view of parasite development (Valero et al., Reference Valero, Panova, Comes, Fons and Mas-Coma2002) and host pathogenicity (Valero et al., Reference Valero, Santana, Morales, Hernandez and Mas-Coma2003, Reference Valero, Navarro, Garcia-Bodelon, Marcilla, Morales, Garcia, Hernandez and Mas-Coma2006, Reference Valero, Girones, Garcia-Bodelon, Periago, Chico-Calero, Khoubbane, Fresno and Mas-Coma2008). Five male Wistar rats (80–100 g) were infected with 20 metacercariae/rat, by use of an orogastric syringe. A balanced commercial rodent diet (Panlab Chow A04) and water were provided ad libitum. Animal care, animal health, body condition and well being were assessed on a weekly basis by means of checking their body weight and the appearance of the fur. Infected animals presented a lower body weight than negative controls at the end of the experiment. No mortality occurred. At 10 weeks post-infection, animals were humanely euthanized with an overdose of the anesthetic (IsoFlo®; Dr Esteve SA, Barcelona, Spain). The worms were obtained in each rat by necropsy.

Collection of excretory/secretory extract of proteins from F. hepatica adult worms

To prepare excretory/secretory products from F. hepatica adults (FhES), liver flukes were collected from Wistar rats. Liver flukes were cultured at concentrations of 1 worm mL−1 of medium for 12 h at 37° C. The medium was collected and centrifuged. After initial centrifugation at low speed to remove larger particles, the supernatant fraction was centrifuged at 15 000 g for 30 min at 4° C, and the supernatant was collected and concentrated to 1 mg mL−1 using an ultrafiltration membrane (YM-3, Amicon).

Plasminogen-binding assay

In order to determine whether the FhES extract has the ability to bind plasminogen, an enzyme-linked immunosorbent assay (ELISA) was performed (González-Miguel et al., Reference González-Miguel, Morchón, Mellado, Carretón, Montoya-Alonso and Simón2012). In brief, multiwell microplates (Costar) were coated with 1 µg per well of FhES extract diluted in carbonate buffer, pH 9.6, overnight at 4 °C. The wells were blocked with 1% BSA in PBS and incubated successively with increasing amounts (from 0 µg to 3 µg) of human plasminogen (Acris Antibodies), with a sheep anti-human plasminogen IgG (Acris Antibodies) at 1:2000 dilution and then with a peroxidase-conjugated donkey anti-sheep IgG (Sigma) at 1:4000 dilution. All incubations were performed for 1 h at 37 °C and between each step washed three times with PBS wash buffer (PBS containing 0.05% Tween20). Ortho-phenylene-diamine was used as a chromogen. Optical densities (OD) were measured at 492 nm in an Easy Reader (Bio-Rad). In parallel, competition assays were performed by including 50 mm of the lysine analogue ε-aminocaproic acid (εACA) during plasminogen incubation. Some wells coated with BSA only were used as negative controls.

Plasmin generation assay

Plasminogen activation and subsequent fibrinolysis was analysed in a test volume of 100 µL by measuring the amidolytic activity of generated plasmin (González-Miguel et al., Reference González-Miguel, Morchón, Mellado, Carretón, Montoya-Alonso and Simón2012). In each well, 2 µg of human plasminogen (Acris Antibodies) were incubated in PBS with 3 µg of the chromogenic substrate D-Val-Leu-Lys 4-nitroanilide dihydrochloride (Sigma) in the presence of 1 µg of FhES. Activation of plasminogen was initiated by the addition of 15 ng of tPA (Sigma). In parallel, plasmin generation was also measured in the absence of t-PA, to observe the ability of the FhES extract proteins of activating plasminogen on their own. Plates were incubated at 37° C for 1 h and the hydrolysis of the chromogenic substrate was analysed by measuring absorbance at 405 nm in an Easy Reader (Bio-Rad).

Two-dimensional electrophoresis of FhES extract

FhES extract was subjected to two-dimensional electrophoresis as described before by us for the antigenic extracts from other parasites (Oleaga et al., Reference Oleaga, Pérez-Sánchez, Pagés, Marcos-Atxutegi and Simón2009; González-Miguel et al., Reference González-Miguel, Morchón, Gussoni, Bossetti, Hormaeche, Kramer and Simón2014). Briefly, FhES extract was purified with the ReadyPrep 2-D Cleanup Kit (Bio-Rad) following the manufacturer's recommendation and resuspended in rehydration buffer 2-D (7 M urea, 2 M thiourea, 4% 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS)). The samples were divided into 125 µL aliquots (containing 60 µg of protein) and stored at −20° C until use. When they were used FhES aliquots were supplemented with ampholytes and 5 M DTT, incubated and centrifuged to remove all particulate material and then applied to 7-cm IPG strips (Bio-Rad) with linear pH ranges of 3–10, 5–8 and 7–10, using a Protean isoelectric focusing (IEF) Cell (Bio-Rad) for IEF. After IEF, strips were reduced and alkylated, and second dimension separation was done in 12% acrylamide gels. Gels were then silver stained with the PlusOne Silver Staining Kit (GE Healthcare), with the Sypro Ruby fluorescent dye (Bio-Rad) for quantitative analysis or transferred to nitrocellulose membranes for their immunoblot analysis. The 2-D images were scanned with the GS-800 Densitometer (Bio-Rad) and analysed with the Quantity One Software v.4.6.5 (Bio-Rad).

Plasminogen ligand blotting assay

To determine which proteins of FhES extract bind plasminogen, they were electrotransferred from 2D gels to nitrocellulose membranes at 20 V for 30 min using a Trans-Blot SD Semi-Dry Transfer cell (Bio-Rad). Blots were blocked with 2% BSA in PBS wash buffer, for 1 h at room temperature. FhES membranes were incubated overnight at 4 °C with 10 µg mL−1 of human plasminogen. Then, the blots were incubated with a sheep anti-human plasminogen IgG (Acris Antibodies) at 1:1000 dilution and with a peroxidase-conjugated donkey anti-sheep IgG (Sigma) at 1:2000 dilution. These incubations were performed at 37° C for 90 min with shaking and between each step washed three times with washing buffer for 5 min per wash. Protein bands were revealed with 4-chloro naphthol. Negative controls were also used in which the plasminogen had been omitted. Membranes were digitized with the scanner GS-800 Densitometer (Bio-Rad) using the Quantity One Software v.4.6.5 (Bio-Rad). Matching of 2-D gels with the homologous Western blot to identify plasminogen-binding proteins, the assignment of molecular weights (MW) and isoelectric points (pI) of each protein were analysed using the PDQuest Software v.8.0.1 (Bio-Rad). All assays were performed in triplicate to assess the reproducibility of the spot pattern.

Mass spectrometry (MS) and protein identification

The spots containing plasminogen-binding proteins were excised manually from the gels and sent to the proteomics facility of SCSIE University of Valencia (Valencia, Spain) for MS analysis. Samples were digested with sequencing grade trypsin (Promega) as previously described (Shevchenko et al., Reference Shevchenko, Jensen, Podtelejnikov, Sagliocco, Wilm, Vorm, Mortensen, Boucherie and Mann1996). The digestion was stopped with 1% trifluoroacetic acid and the digested peptides were concentrated. A BSA plug was analysed in the same way to control the digestion process.

The resulting mixtures were analysed in a 5800 MALDI TOF/TOF (ABSciex) in positive reflector mode. Five of the most intense precursors (according to the threshold criteria: minimum signal-to-noise: 10, minimum cluster area: 500, maximum precursor gap: 200 ppm, maximum fraction gap: 4) were selected for every position for the MS/MS analysis. And, MS/MS data were acquired using the default 1 kV MS/MS method. The MS-MS/MS information was sent to MASCOT via the Protein Pilot (ABSciex). Database search was performed on the NCBI database. Searches were done with tryptic specificity allowing one missed cleavage and a tolerance on the mass measurement of 100 ppm in MS mode and 0.8 Da in MS/MS mode. Carbamidomethylation of Cys was used as a fixed modification and oxidation of Met and deamidation of Asn and Gln as variable modifications.

When a positive identification was not achieved, spots were analysed by liquid chromatography and tandem MS (LC–MS/MS). In this case, 5 µL of every sample was loaded onto a trap column (NanoLC Column, 3μ C18-CL, 350 um × 0.5 mm; Eksigent) and desalted with 0.1% trifluoroacetic acid at 3μL per min during 5 min. The peptides were then loaded onto an analytical column (LC Column, 3 µ C18-CL, 75 um × 12 cm, Nikkyo) equilibrated in 5% acetonitrile and 0.1% formic acid. Elution was carried out with a linear 5–45% gradient of solvent B (95% acetonitrile, 0.1% formic acid) at a flow rate of 300 nL per min. Peptides were analysed in a mass spectrometer nanoESI-Q-TOF (5600 TripleTOF, ABSciex). The tripleTOF was operated in information-dependent acquisition mode, in which a 0.25-s TOF MS scan from 350–1250 m z−1, was performed, followed by 0.05-s product ion scans from 100 to 1500 m z−1 on the 50 most intense 2–5 charged ions. ProteinPilot default parameters were used to generate peak list directly from 5600 TripleTOF wiff files. The Paragon algorithm of ProteinPilot was used to search NCBI protein database with the following parameters: trypsin specificity, iodoacetamide cys-alkylation and the search effort set to rapid. To avoid using the same spectral evidence in more than one protein, the identified proteins are grouped based on MS/MS spectra by the Protein-Pilot Progroup algorithm. Thus, proteins sharing MS/MS spectra are grouped, regardless of the peptide sequence assigned. The protein within each group that can explain more spectral data with confidence is shown as the primary protein of the group. Only the proteins of the group for which there is individual evidence (unique peptides with enough confidence) are also listed, usually toward the end of the protein list. Finally, the mascot generic files obtained for every spot by Protein Pilot were sent to MASCOT. Database search was performed on the NCBI TREMATODA-ESTs database. The molecular function and biological processes of the identified proteins were assigned according to the gene ontology database (http://www.geneontology.org) and the Swiss-Prot/UniProt database (http://beta.uniprot.org).

Statistical analysis

The results from the plasminogen-binding assay and plasminogen activation assay were analysed with the Student's t-test. The results were expressed as the mean ± s.e.m. of at least three independent experiments. In all experiments, a significant difference was defined as a P value of <0.05 for a confidence level of 95%.

Results

Proteins of FhES extract bind plasminogen

The binding level of plasminogen to FhES extract was assessed by ELISA (Fig. 1). Analyses showed that proteins of FhES extract bind plasminogen and that this binding is directly proportional to the amount of plasminogen. The negative control consisting of wells coated only with BSA showed some non-specific binding activity, but always with values significantly lower than those obtained by FhES (P < 0.05). To determine whether or not lysine residues are involved in binding, a competition experiment including 50 mm εACA was carried out. In this case, the binding was inhibited about 60% resulting in slightly higher optical densities than the negative control (Fig. 1).

Fig. 1. Plasminogen binding to 1 µg of FhES extract measured over a range of plasminogen amounts using a microtitre plate method: (■) Incubation with increasing amounts of plasminogen, 0–3 µg. (●) Competition assay with 50 mm εACA included during plasminogen incubation. (▲) Negative control consisted of wells coated only with BSA. Each point is the mean of three replicates ± s.d. The asterisk (*) designates significant (P < 0.05) differences.

Proteins of FhES extract enhance the activation of plasminogen by tPA

In order to assess the ability of FhES extract to activate plasminogen and generate plasmin on their own, the amidolytic activity of plasmin generated in the presence or absence of tPA was measured. Negative controls replacing FhES for BSA or tPA were also used. Figure 2 shows the capacity of proteins of FhES to stimulate plasmin generation by tPA obtaining optical densities significantly higher than the negative controls (P < 0.05). Furthermore, this effect is inhibited by 50 mm εACA, indicating the involvement of lysine residues in the process. Plasminogen-activation did not occur in the absence of tPA.

Fig. 2. Plasminogen activation and plasmin generation by FhES extract of Fasciola hepatica: (□) 15 ng of t-PA was added to mixtures containing 2 µg of human plasminogen, 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride (Sigma) and 1 µg of FhES extract (or BSA as negative control) in the presence or absence of 50 mm of εACA in a test volume of 100 µL. (■) No t-PA was added to reaction mixtures. Each point is the mean of three replicates ± s.d. The asterisk (*) designates significant (P < 0.05) differences.

Separation of proteins of FhES extract by two-dimensional electrophoresis

To obtain an overall view of all the proteins of the FhES extract, it was first electrofocused using 3–10 linear immobilized pH gradient strips. Silver nitrate staining of these 2-D gels revealed 341 spots in the excretome of F. hepatica with pIs between 5 and 9.7, and a broad range of MWs (10–150 kDa). Only 33 spots were observed with pI <5 (Fig. 3A).

Fig. 3. Two-dimensional electrophoresis of the FhES and ligand blotting with plasminogen: (A) Representative 2-DE of 60 µg of the FhES extract from adult F. hepatica flukes. The gels were in the 3–10 pH range, 12% polyacrylamide and silver-stained. (B) Ligand blotting assay to determine which proteins of FhES extract bind plasminogen. The plasminogen-binding spots revealed are circled and numbered. Reference molecular masses are indicated on the left.

In order to improve spot resolution and detection, once the spot MW and pI ranges were determined, the FhES extract was electrofocused in 5–8 and 7–10 IPG strips. With these new conditions, silver staining revealed only 168 spots, most of them (119) located between pH 5 and 8 (not shown). We proceeded to use linear pH ranges of 3–10 for IEF of the FhES extract in the following experiments, due to the loss of resolution by using narrower pH ranges.

Identification of plasminogen-binding proteins

To identify plasminogen-binding proteins, a ligand blotting with plasminogen of 2D gels of 3–10 pH was performed. As shown in Fig. 3B, a total of 78 plasminogen-binding spots were revealed (22.87% for total spots revealed in the excretome of F. hepatica). All of them were resolved in a range of MWs and pIs between 20 and 90 kDa, and 4.8 and 8.7, respectively. In the control blots, in which plasminogen incubation was omitted, the anti-plasminogen antibody did not reveal any spots (not shown).

The matching of spots revealed by ligand-blotting with their homologous in the silver-stained 2-D gels allowed us to select 47 representative plasminogen-binding spots of F. hepatica, which were manually excised from 2-D gels and submitted to analysis by MS.

Table 1 shows the identity of these proteins, the number of access to similar information available in the NCBI database and their MWs and pIs (theoretical and experimental). All of the spots were identified (n = 47) and corresponded to 16 different proteins. Between 1 and 6 isoforms of each protein were identified. Most of the identified spots corresponded to proteins of F. hepatica deposited in databases (38 of 47 spots). The 9 remaining spots corresponded to proteins from other trematodes (Schistosoma haematobium, Schistosoma japonicum, Clonorchis sinensis and Opistorchis viverrini). In addition, Table 1 shows the molecular function and biological processes in which the 16 proteins identified are involved. Among these, most were related to energy generation and metabolic pathways (6 proteins/16 spots), cell redox homeostasis or detoxification (5 proteins/13 spots) and to proteolysis (3 proteins/15 spots).

Table 1. Plasminogen-binding spots of the FhES extract identified by MALDI-TOF/TOF or LC–MS/MS

Exp, experimental; theo, theoretical.

Quantitative analysis of proteins of FhES extract by Sypro Ruby fluorescent dye

Two-dimensional electrophoresis gels were stained with Sypro Ruby fluorescent dye in order to perform a quantitative analysis using the PDQuest Software v.8.0.1 (Bio-Rad) and to select the most abundant plasminogen-binding spots in the excretome of F. hepatica. The most abundant proteins were resolved in a narrow range of MWs and pIs (between 22 and 68 KDa, and 4.7 and 6, respectively) (Fig. 4). The ten plasminogen-binding spots with the higher optical density after Sypro Ruby staining and, therefore, relatively more abundant in the FhES extract were spots number 23, 40, 42, 45, 60–64 and 67 and corresponded to different isoforms of cathepsin L, cathepsin L1D, enolase y glutathione S-transferase (see Table 1).

Fig. 4. Quantitative analyses of the FhES extract by Sypro Ruby florescent dye: Representative 2-DE of 60 µg of the FhES extract from adult F. hepatica flukes. The gels were in the 3–10 pH range, 12% polyacrylamide and Sypro Ruby-stained. Note density of proteins as the appearance of clear spots on a dark background, which is directly proportional to the amount of each protein into the gel. The plasminogen-binding spots revealed on the ligand blotting assay are circled and numbered.

Discussion

Plasminogen-binding proteins in the Fasciola hepatica secretome/excretome

Plasmin produced by the activation of plasminogen is a broad-spectrum enzyme whose proteolytic activity, in addition to degrading the fibrin clot, has been linked to the lysis of extracytoplasmic matrices (Vassalli et al., Reference Vassalli, Sappino and Belin1991). This fact has allowed to relate the secretion of plasminogen receptors carried out by different groups of parasites with their dissemination, establishment and survival in host tissues (González-Miguel et al., Reference González-Miguel, Siles-Lucas, Kartashev, Morchón and Simón2016). Regarding trematodes, their interaction with the fibrinolytic system of their host has been widely studied in schistosomes (Mebius et al., Reference Mebius, van Genderen, Urbanus, Tielens, de Groot and van Hellemond2013), while several proteins with plasminogen-binding capacity, among them an enolase in the excretory/secretory products of F. hepatica (Bernal et al., Reference Bernal, de la Rubia, Carrasco-Abad, Toledo, Mas-Coma and Marcilla2004), have been identified in S. mansoni, S. japonicum, S. bovis, Clonorchis sinensis or Echinostoma caproni (Marcilla et al., Reference Marcilla, Pérez-García, Espert, Bernal, Muñoz-Antolí, Esteban and Toledo2007; Ramajo-Hernández et al., Reference Ramajo-Hernández, Pérez-Sánchez, Ramajo-Martín and Oleaga2007; de la Torre-Escudero et al., Reference de la Torre-Escudero, Manzano-Román, Pérez-Sánchez, Siles-Lucas and Oleaga2010, Reference de la Torre-Escudero, Manzano-Román, Siles-Lucas, Pérez-Sánchez, Moyano, Barrera and Oleaga2012; Yang et al., Reference Yang, Qiu, Xia, Yao, Fu, Yuan, Feng and Lin2010; Wang et al., Reference Wang, Chen, Hu, Deng, Zhou, Lv, Fan, Men, Huang, Sun, Hu, Chen, Yang, Liang, Zheng, Hu, Xu, Wu and Yu2011; He et al., Reference He, Ren, Chen, Wang, Li, Lin, Liang, Liang, Hu, Lei, Bian, Huang, Wu, Li and Yu2014; Hu et al., Reference Hu, Zhang, Huang, Li, Liang, Wang, Xu, Huang and Yu2014; Figueiredo et al., Reference Figueiredo, Da'dara, Oliveira and Skelly2015).

In the present work, we demonstrate that the FhES extract binds plasminogen and is capable of stimulating the generation of plasmin in an in vitro system. Activation of plasminogen only occurs in the presence of the fibrinolytic activator tPA, which is consistent with previously obtained results with antigens secreted by other parasites (Figuera et al., Reference Figuera, Gómez-Arreaza and Avilán2013). In addition, competition assays performed with ε-ACA acid reveal the involvement of the lysine residues of the FhES proteins in the process. This has particular relevance since the involvement of this type of residues has been described in the interaction with plasminogen (Rijken and Lijnen, Reference Rijken and Lijnen2009).

The proteomic analysis of the FhES extract together with the mass spectrometry allowed the identification of 47 plasminogen-binding spots, which corresponded to 16 different proteins. The large percentage of identification was made possible by the existence of available information on F. hepatica proteins in the databases, as a result of the recent publication of the parasite genome (Cwiklinski et al., Reference Cwiklinski, Dalton, Dufresne, La Course, Williams, Hodgkinson and Paterson2015). Among them, the glycolytic enzymes enolase, glyceraldehyde phosphate-dehydrogenase and fructose bisphosphate-aldolase have been extensively studied for their role as plasminogen-binding proteins in a large number of pathogenic species of fungi, bacteria and parasites (Stie et al., Reference Stie, Bruni and Fox2009; de la Paz Santangelo et al., Reference de la Paz Santangelo, Gest, Guerin, Coinçon, Pham, Ryan, Puckett, Spencer, Gonzalez-Juarrero, Daher, Lenaerts, Schnappinger, Therisod, Ehrt, Sygusch and Jackson2011; Bhattacharya et al., Reference Bhattacharya, Ploplis and Castellino2012; Funk et al., Reference Funk, Schaarschmidt, Slesiona, Hallström, Horn and Brock2016; González-Miguel et al., Reference González-Miguel, Siles-Lucas, Kartashev, Morchón and Simón2016). Among the remaining metabolic proteins identified, triose phosphate isomerase had previously been linked to the fibrinolytic pathway (Marcilla et al., Reference Marcilla, Pérez-García, Espert, Bernal, Muñoz-Antolí, Esteban and Toledo2007; Furuya and Ikeda, Reference Furuya and Ikeda2011), while others have been identified as plasminogen-binding proteins for the first time: glutamate dehydrogenase and hypothetical protein T265_01567.

Cathepsins are also described for the first time as plasminogen receptors in pathogens (15 isoforms identified). Cathepsins are a large family of cysteine endopeptidases abundantly expressed in the secretions of F. hepatica (McVeigh et al., Reference McVeigh, Maule, Dalton and Robinson2012). Their important role in such important processes as host haemoglobin digestion for nutrition, invasion or migration justify that they have been used as therapeutic targets in vaccine trials (Meemon and Sobhon, Reference Meemon and Sobhon2015). In addition, the expression associated with Fasciola development of these proteases has been correlated with the passage of the parasite through host tissues (Stack et al., Reference Stack, Dalton and Robinson2011).

Another widely represented group (13 isoforms, 5 proteins) and whose interaction with plasminogen is also documented here for the first time in parasites are proteins related to redox or detoxifying homeostasis (see Table 1). These molecules have been linked to mechanisms of immune evasion and to the protection of F. hepatica from the harmful reactive oxygen species released by host immune cells. In addition, the secretion of a wide variety of antioxidants depending on the migration of Fasciola through host tissues has been documented (Robinson et al., Reference Robinson, Menon, Donnelly, Dalton and Ranganathan2009).

The results of this work demonstrate that F. hepatica has a large repertoire of plasminogen-binding proteins, which could be translated as a redundant system that reveals the importance of plasminogen recruitment for this parasite. These antigens are also among the most abundant within the excretoma of F. hepatica as revealed by the quantitative analysis (see Fig. 4). The proteins identified have important functions related to energy generation or detoxification processes but may perform other important functions such as plasminogen binding when they are at the host/parasite interface. In this sense, recent studies have begun to study these moonlighting functions of parasitic glycolytic enzymes (such as enolase or GAPDH) when they are found in extracellular localization related to invasion or establishment in the host (Gómez-Arreaza et al., Reference Gómez-Arreaza, Acosta, Quiñones, Concepción, Michels and Avilán2014).

The plasminogen-binding proteins identified in the present work are part of an extract of F. hepatica adult worms. However, many of them, such as enolase, GAPDH, FBAL, cathepsins L and L1, GST, thrioredoxin or Fh15, remain active from the immature stage of the parasite (Halton, Reference Halton1997; McVeigh et al., Reference McVeigh, Maule, Dalton and Robinson2012) so that the obtained results could allow to relate the fibrinolytic activation with the migration carried out by the juvenile forms of the parasite. Recruitment of plasminogen by the adult phases of F. hepatica and, therefore, the use of plasmin could be linked with its survival in bile ducts, as has already been related in other trematodes such as C. sinensis (Wang et al., Reference Wang, Chen, Hu, Deng, Zhou, Lv, Fan, Men, Huang, Sun, Hu, Chen, Yang, Liang, Zheng, Hu, Xu, Wu and Yu2011; He et al., Reference He, Ren, Chen, Wang, Li, Lin, Liang, Liang, Hu, Lei, Bian, Huang, Wu, Li and Yu2014), or with the clot-prevention necessary for blood nutrition described in F. hepatica (Halton, Reference Halton1997).

Baseline of neurological, meningeal and ocular disorders in fascioliasis patients

In patients showing infection by Fasciola in the liver, serious neurological, meningeal and ocular symptoms have been reported, such as limb and facial paralysis including hemiplegia, paresis up to even tetraparesis, impressive walking problems and movement disorders, paraesthesia, speech disorders, loss of senses, convulsions, epilepsia and coma, confusion, disorientation and amnesia, visual hallucinations, extremely intense nystagmus, or permanent blindness, among many others (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b). Such patients should not be confused with others presenting similar disorders due to neurofascioliasis or intracranial infection and ophthalmofascioliasis or direct affection of the eye by migrating ectopic flukes. The latter two ectopic infections appear to be rare (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b).

A baseline obtained when comparing the clinical pictures in these neurological patients showing infection by Fasciola in the liver offers crucial information. Such disorders have been reported in patients of both sexes, all age groups and whether at the acute phase (sometimes even very early within the first symptoms) or in the chronic or advanced chronic phase (even several years after infection). Moreover, such neurological manifestations have been described in patients infected by both species of Fasciola and from throughout the world. This indicates that there is no relationship with Fasciola species nor with geographical strains of the liver flukes (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b).

The capacity of flukes in the liver to cause the aforementioned disorders at distance means their excretory/secretory products to be able to alter the blood-brain barrier. Surprisingly, in the patients affected by such neurological disorders in whom the fluke burden could be assessed, infection was by a very low number of flukes in the liver, namely between 1 and 12 flukes per subject suffering major neurological symptoms, with an exception of a neurological fatal case in whom 26 flukes were found (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b). Consequently, a priori it does not seem to be a direct relationship between burden and capacity to give rise to neurological disorders (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b), suggesting that individual host susceptibility may perhaps underlie the appearance of such disorders in given patients. Markers have already proved to be useful in human fascioliasis (Valero et al., Reference Valero, Bargues, Khoubbane, Artigas, Quesada, Berinde, Ubeira, Mezo, Hernandez, Agramunt and Mas-Coma2016) and the availability of indicators allowing for the detection of patient susceptibility to suffer from such neurological disorders would, therefore, be welcome.

Blood-brain barrier leakage in Fasciola infection

A study in the laboratory rat model demonstrated that (i) F. hepatica infection in the liver was able to impact on remote tissues, (ii) an altered neural nucleotide signature was one of the strongest metabolic responses in infected rats and (iii) the perturbed immunological function suggested by the altered nucleotide levels in the brains of F. hepatica-infected animals proved the neural effects were exclusive of Fasciola when compared with other trematodes as S. mansoni and E. caproni (Saric et al., Reference Saric, Li, Utzinger, Wang, Keiser, Dirnhofer, Beckonert, Sharabiani, Fonville, Nicholson and Holmes2010). Significant perturbations of the nucleotide balance in the brain, together with an increase of an important direct negative regulator of inflammatory cytokines in macrophages such as plasma IL-13, were found. This suggested a shift toward modulation of immune reactions by secretion of nucleotide-degrading enzymes to minimize inflammatory response, which may favour the co-existence of the parasite in the host. The pattern of differentiating metabolites in the neurochemical profiles was composed of a significant increase in the relative levels of inosine, tyrosine and phenylalanine. Conversely, the relative tissue concentrations of GPC, succinate, inosine mono-, di- and triphosphate, adenosine and adenosine mono-, di- and triphosphate were lower in the brains of infected laboratory rats (Saric et al., Reference Saric, Li, Utzinger, Wang, Keiser, Dirnhofer, Beckonert, Sharabiani, Fonville, Nicholson and Holmes2010).

In individual case reports, many mechanisms have been observed to accompany or suggested to be linked to the neurological disorders described in given patients presenting infection by Fasciola in the liver (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b): inflammation, vasculitis, arteritis, thrombosis; local and systemic responses; circulating immune complexes and complement system activation; autoimmune responses; hypersensivities, type III hypersensivity, Arthus reaction; impairment of the blood-brain barrier; hepatic encephalopathy and relationships with ammonia, sodium, manganese and others; hypereosinophilia-induced encephalopathy; proinflammatory cytokines, oxidative/nitrative stress; neurotoxins; substances derived from the damage, malfunction and failure of the liver; coinfections and sepsis. This suggests a large complexity and inter-relationships of immuno-allergic and physiopathogenic processes (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b). Interestingly, a neurological picture caused by Fasciola has also been found associated to a condition such as the antiphospholipid syndrome (Frances et al., Reference Frances, Piette, Saada, Papo, Wechsler, Chosidow and Godeau1994), whereas this in its turn was later also related to plasmin and the fibrinolytic system (Yang et al., Reference Yang, Hwang, Yan, Gallagher, FitzGerald, Grossman, Hahn and Chen2004).

We here demonstrate that the excretory/secretory products of Fasciola may trigger a plasmin-dependent mechanism or pathway which increases blood-brain barrier permeability and cause neurological disorders.

Fasciola hepatica is able to interact with the fibrinolytic system of its host by secreting a large number of proteins with the ability to bind plasminogen and enhance the generation of plasmin. Worth mentioning is that most of these proteins (38 out of 47 spots) appear to be exclusive for F. hepatica so far, including a large number of cathepsin isoforms. The potential use of some of these antigens as novel targets for anthelmintic compounds and potential specific multi-antigenic vaccine candidates should be explored in the future.

The remaining few of the plasminogen-binding proteins identified in the present work are common to those that have been studied in other pathogens with very different host invasion strategies. This may be probably related to a process of evolutionary convergence, which determines the appearance of similar mechanisms to solve common problems.

Fluke behaviour in the acute and chronic phases

Fasciola metacercariae infect humans after ingestion together with edible freshwater vegetables, drinking natural contaminated water, or terrestrial plants, fruits and tubercles washed with contaminated water (Mas-Coma et al., Reference Mas-Coma, Bargues and Valero2018). Metacercariae excyst in the small intestine within an hour after ingestion. A proportion of metacercariae die in the gastrointestinal tract and relatively few eventually penetrate the host's intestine wall, thus starting their active migration.

This acute phase, also called invasive or migratory phase, comprises intraorganic migration until final penetration into the bile ducts where they become sexually mature (Fig. 5A). Fecundation, egg production and shedding by the mature worms, passive transport of the eggs with the bile up to the intestinal lumen and appearance of eggs in stools take at least 3–4 days. The pre-patent period includes from the ingestion of metacercariae to the first appearance of eggs in feces and takes at least 3–4 months in humans. This time may, therefore, be extrapolated to the duration of the migratory phase (shorter than the prepatent period in only 3–4 days) (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b). This phase is 1–2 weeks longer when in F. gigantica infection (Valero et al., Reference Valero, Bargues, Khoubbane, Artigas, Quesada, Berinde, Ubeira, Mezo, Hernandez, Agramunt and Mas-Coma2016).

Fig. 5. Schematic representation of the interaction between infecting Fasciola behaviour, fibrinolysis system alterations triggering subsequent blood-brain barrier leakages and contact system alterations inducing systemic vasculitis: (A) In the acute phase of the disease in cases of many simultaneously migrating, small-sized juvenile flukes after ingestion of numerous metacercariae. (B) In the chronic phase of the disease after the release of large amounts of accumulated excretory/secretory products following the breakage of encapsulating formations triggered by single worm tracks at different times. (C) Blood-brain barrier leakages subsequently occurring due to fibrinolytic system-dependent mechanism involving plasmin-dependent generation of bradykinin and subsequent activation of bradykinin B2 receptors, according to different plasminogen-binding protein agglomeration waves. (D) Inflammation and dilation of blood vessels due to contact system-dependent generation of the proinflammatory peptide bradykinin. Schema design and drawing by S. Mas-Coma.

The initial penetration of the juvenile worms through the duodenum or jejunum walls may give rise to focal haemorrhages and inflammation. Juvenile worms appear in the abdominal cavity by about 2 h after ingestion. Afterwards, they migrate inside the peritoneum until contacting the liver. During this migration (Fig. 5A), the juvenile worms cause the extensive mechanical destruction of abdominal peritoneum and liver tissue causing intensive haemorrhagic lesions and immunological and inflammatory reactions, with localized or generalized toxic and allergic processes. Most reach the liver within 6 days after excystment. Juvenile flukes migrate through the liver parenchyma for 5–6 weeks, preferentially feeding directly on liver tissue. They finally penetrate into the bile ducts where they become sexually mature. Migratory parasites become sometimes trapped in the liver parenchyma and die without reaching the bile ducts, leaving cavities filled with necrotic debris and considerable liver areas may subsequently be replaced by scar tissue (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b). It has also been speculated that immature flukes may enter the blood stream and be carried to various parts of the body, or may reach the liver by travelling up the bile duct. In the case of failure to reach the biliary system of the liver, the immature flukes may die in the abdominal cavity and other parts of the body (Chen and Mott, Reference Chen and Mott1990).

This chronic phase, also called biliary or obstructive phase (Fig. 5B), starts from the moment the flukes penetrate in the bile ducts, that is, 3–4 days before the first appearance of eggs in stools. Consequently, the presence of eggs in stools indicates the patient to be in that chronic phase. This phase may last for a very long time according to the adult worm life span of up to 13.5 years in humans (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b). The term ‘advanced chronic’ phase is used for long-lasting infections, when ingestion of metacercariae took place a long time ago, e.g. 1 or more years before patient diagnosis.

Once in their final hepatic microhabitat, the endocrine system of the host provides reliable signals that are part of the perceptual world of the blood-feeding Fasciola adult (Sukhdeo et al., Reference Sukhdeo, Sangster and Mettrick1988; Sukhdeo and Sukhdeo, Reference Sukhdeo and Sukhdeo1989). Adult flukes in the bile ducts cause inflammation, hyperplasia of the epithelium and thickening and dilatation of duct and gall bladder walls.

Mature worms living inside the biliary canals may re-enter the liver parenchyma in different moments of the chronic phase (Han et al., Reference Han, Jang, Choi, Kim, Kim, Won, Kim and Cho1999), return back to a biliary canal after a more or less short transit, or give rise to an encapsulating eosinophilic granuloma if staying in the parenchyma for a longer period (Fig. 5B). This may explain the findings of eggs of both F. hepatica and F. gigantica inside eosinophilic granulomas in the liver parenchyma (Arjona et al., Reference Arjona, Riancho, Aguado, Salesa and Gonzalez-Macías1995) or hepatic masses (Grange et al., Reference Grange, Dhumeaux, Couzineau, Bismuth and Bader1974), although the possibility cannot be discarded that such worms were migrating flukes which never achieved a biliary canal at the end of their initial migration as juveniles and finished to mature inside the parenchyma. The finding of high numbers of Fasciola eggs in eosinophilic granulomas from peritoneum and intestinal wall suggest juvenile worms becoming encapsulated in granulomas in extrahepatic sites at the initial steps of their migratory pathway and subsequent capacity to mature and produce and shed eggs after selfing ‘in situ’ (Mohammadi Ghalehbin et al., Reference Mohammadi Ghalehbin, Chinifroush Asl and Ramzi2010).

Interactions between fluke behaviour and the fibrinolysis system

This capacity of Fasciola to secrete a large number of proteins with the ability to bind plasminogen and enhance the generation of plasmin may also underlie the leakage of the blood-brain barrier and subsequent induction of neurological disorders. There appear to be multifactorial and probably plasminogen-dependent effects of tPA on the blood-brain barrier (Niego et al., Reference Niego, Freeman, Puschmann, Turnley and Medcalf2012; Niego and Medcalf, Reference Niego and Medcalf2014). Chronic tPA overexpression has been seen to increase the permeability of the intact blood-brain barrier by a plasminogen-dependent mechanism involving plasmin-dependent generation of the proinflammatory peptide bradykinin and subsequent activation of bradykinin B2 receptors. It is possible that chronic vs acute increases in tPA levels in the circulation act by different mechanisms. It has been speculated that the initial blood-brain barrier leakage induced by bradykinin allows circulating tPA to enter the brain parenchyma, where it can exert further deleterious effects on blood-brain barrier integrity and glutamatergic transmission (Marcos-Contreras et al., Reference Marcos-Contreras, Martinez de Lizarrondo, Bardou, Orset, Pruvost, Anfray, Frigout, Hommet, Lebouvier, Montaner, Denis Vivien and Gauberti2016).

In patients in the invasive acute phase, presenting with neurological symptoms sometimes appearing suddenly and very early, the induction of neurological disorders is unavoidably caused by juvenile flukes migrating through different tissues. The rarity of these situations may be due to the infection by concomitant ingestion of numerous metacercariae giving rise to many juvenile flukes migrating simultaneously. An E/S product release sufficient for a blood-bain barrier leakage may happen in such unusual cases of a high number of small-sized, simultaneously migrating juvenile worms of around 250 µm in length (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b). This may explain the early appearance of neurological disorders even within the first disease symptoms (Fig. 5A).

Indeed, the very precocious and high activity of the migrating juveniles has already been assessed from the immunological point of view. By the time the newly excysted juveniles have penetrated the intestinal wall and entered the peritoneum, they have already initiated the immune events that will dominate throughout infection. So, these early-stage parasites secrete immunomodulatory molecules that influence the function of innate cells in the intestinal wall and peritoneal cavity. A systemic antigen-specific Th2 response is firmly established already at 7 days postinfection (Dalton et al., Reference Dalton, Robinson, Mulcahy, O'Neill and Donnelly2013).

In patients in the biliary chronic phase, even including neurological pictures appearing in the long term (many years after the infection), the induction of neurological disorders should be the consequence of other causes. In these patients, such a sufficient E/S product release may not depend on a massive Fasciola infection but on a very few, or perhaps single, large-sized adult worms. In the biliary canals, they have been reported to attain up to a length of 29 mm in F. hepatica and 52 mm in F. gigantica in cattle (Periago et al., Reference Periago, Valero, Panova and Mas-Coma2006). In ectopic worms found in patients presenting with neurological manifestations, a maximum length of up to 8 mm has been reported (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b). The tracks followed by the worms and the subsequent physiopathological changes suffered by these tracks may allow for the accumulation of excretory/secretory products inside. These tracks give rise to numerous small holes and cavities and cause inflammation, nodular lesions, abscesses, cystic cavities, haemorrhages, necrosis, granulomas, fibrosis and formations described as liver mass. The breakage of such formations in the liver or ectopically in other neighbouring organs in the patient at different times in the chronic and advanced chronic phases may give rise to a simultaneous release of large amounts of accumulated excretory/secretory products (Fig. 5B).

Whole worms have been sometimes found inside these different types of lesions (Ghawabi et al., Reference Ghawabi, Salem and Azab1978; Ragab and Farag, Reference Ragab and Farag1978; Acosta-Ferreira et al., Reference Acosta-Ferreira, Vercelli-Retta and Falconi1979; Park et al., Reference Park, Ro, Kim and Gutierrez1984; Aroonroch et al., Reference Aroonroch, Worawichawong, Nitiyanant, Kanchanapitak and Bunyaratvej2006; Kim et al., Reference Kim, Choi, Choi, Shin, Park, Kim, Choi, Han, Kim, Chu and Park2015). However, worm absence inside such formations is the most current observation reported from patients, indicating that these intratissular worms are able to escape from such host reactions. Mechanical tissue damage by the worms themselves during their tunnel boring, any accidental traumatism, or even perhaps their diffusion by permeability after tissue regeneration, may be at the origin of a quick, timely concentrated release of such E/S products and their access to the circulation.

Hyperfibrinolysis is the consequence of an imbalance between profibrinolytic factors, as tPA and uPA and antifibrinolytic factors, such as plasminogen activator inhibitors, a2-antiplasmin, or thrombin activatable fibrinolysis inhibitor (Shakur et al., Reference Shakur, Roberts, Bautista, Caballero, Coats, Dewan, El-Sayed, Goigichaishvili, Gupta, Herrera, Hunt, Iribhogbe, Izurieta, Khamis, Komolafe, Marrero, Mejia-Mantilla, Mirana, Morales, Olaomi, Olldashi, Perel, Peto, Romana, Ravi and Yutthakasemsunt2010). The most evident pathological effect of hyperfibrinolysis is an increased bleeding (Roullet et al., Reference Roullet, Freyburger, Labrouche, Norisse, Stecken, Quinart, Laurent and Sztark2015). The hyperfibrinolytic state was found to be the result of an increased level of profibrinolytic factors that is not (or not fully) compensated by an increased level of antifibrinolytic factors (Gando, Reference Gando2015). This imbalance triggers the plasmin generation and leads to a severe bleeding tendency.

In fascioliasis, among the highly severe complications described in patients, there are reports on haemorrhages and haematomas (Lortat-Jacob et al., Reference Lortat-Jacob, Maillard, Negevand and Camus1960; Piquet et al., Reference Piquet, Elhadad, Slama and Brassier1986; Sauvage et al., Reference Sauvage, Dhers, Sauzedde, Bakhti and Filippi1996; Martinez et al., Reference Martinez, Rodriguez, Pila-Perez, Pila-Pelaez and Tamakloe2002; Loja et al., Reference Loja, Alvizuri, Vilca, Aviles and Sanchez2003; Rosas et al., Reference Rosas, Fiestas, Torres, Aita and Lozada2008; Morales et al., Reference Morales, Arriagada, Salas, Morales, Fuentes and Santana2009), as well as bleeding (Goodman et al., Reference Goodman, Henderson and Cullity1973), several of them fatal cases reported from children (Bannerman and Manzur, Reference Bannerman and Manzur1986; Acuña-Soto and Braun-Roth, Reference Acuña-Soto and Braun-Roth1987; Almendras-Jaramillo et al., Reference Almendras-Jaramillo, Rivera-Medina, Seijas-Mogrovejo and Almendras-Jaramillo1997). In none of these cases were neurological manifestations reported.

However, in fascioliasis patients presenting with neurological disorders, bleeding has been only described in patients suffering from neurofascioliasis or intracranial infection by migrating ectopic flukes (Ying et al., Reference Ying, Xiaosu and Bin2007; Zhou et al., Reference Zhou, Luo, You, Wang, Xu, Liao, Hui and Cai2008), but in no case of patients presenting with neurological, meningeal, psychiatric or neuropsychic manifestations and/or ocular disorders caused at distance by flukes infecting the liver (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b). This appears to be similar to observations in a cohort of patients with ischemic stroke, among whom none presented hemorrhagic transformations (Marcos-Contreras et al., Reference Marcos-Contreras, Martinez de Lizarrondo, Bardou, Orset, Pruvost, Anfray, Frigout, Hommet, Lebouvier, Montaner, Denis Vivien and Gauberti2016).

This suggests that, in such fascioliasis patients, the balance between profibrinolytic and antifibrinolytic factors may not be altered or sufficiently modified at a systemic level. This does not mean, however, that a circulating plasmin agglomeration may be able to induce timely punctual leakages on the blood-brain barrier (Fig. 5C). Subsequent blood stream passes of such a (or other) plasmin agglomerations may be responsible for blood-brain barrier leakage on different sites, thus underlying triggering of different neurological manifestations. This would, moreover, allow to explain the puzzling polymorphisms and disconcerting multifocality of the neurological manifestations throughout time (Mas-Coma et al., Reference Mas-Coma, Agramunt and Valero2014b).

Inflammation and vasculitis in fascioliasis patients

Recent studies have linked an excess of plasmin result of a long-term pro-fibrinolytic parasite activity with pathogenic mechanisms that include cell proliferation and migration, inflammation and degradation of the extracellular matrix (González-Miguel et al., Reference González-Miguel, Morchón, Carretón, Montoya-Alonso and Simón2015, Reference González-Miguel, Siles-Lucas, Kartashev, Morchón and Simón2016). Consequently, the excess of plasmin generated, a priori beneficial to F. hepatica, could also be related to the typical inflammatory processes of fascioliasis.

tPA is a molecule with dual functions: (i) a serine protease, playing a pivotal role in the homeostasis of blood coagulation/fibrinolysis and extracellular matrix regulation; (ii) a cytokine, executing multiple actions by binding to its membrane receptors and triggering profound intracellular signaling events (Lin and Hu, Reference Lin and Hu2014). tPA appears to have broad implication in the modulation of infiltration and inflammation in diverse organs. The increased inflammatory infiltration in most disease models is accompanied by the concomitant induction of tPA, suggesting that tPA may be a common endogenous factor that modulates inflammatory infiltration and response in multiple organ systems (Lin and Hu, Reference Lin and Hu2014).

Of particular interest appear to be the role of tPA in cerebral inflammation (Wang et al., Reference Wang, Tsirka, Strickland, Stieg, Soriano and Lipton1998; Zhang et al., Reference Zhang, Polavarapu, She, Mao and Yepes2007, Reference Zhang, An, Strickland and Yepes2009) and in liver inflammation (Higazi et al., Reference Higazi, El-Haj, Melhem, Horani, Pappo, Alvarez, Muhanna, Friedman and Safadi2008). The role of tPA has also been investigated in infection by pathogens. Results indicate that tPA may also play an important role in the modulation of innate immunity, which is fundamental in the host defense against pathogens (Lin and Hu, Reference Lin and Hu2014).

In fascioliasis patients presenting with neurological, meningeal and/or ophthalmic manifestations, inflammatory cell reactions in the liver have been reported several times (Paraf et al., Reference Paraf, Rousset, Trad and Benchettrit1967; Lesecq et al., Reference Lesecq, Gnamey, Dubois, Farriaux, Vernes, Capron and Fontaine1972; Aguirre Errasti et al., Reference Aguirre Errasti, Merino Angulo, Flores Torres and De los Ríos1981; Berenger, Reference Berenger1984; Prociv et al., Reference Prociv, Walker and Whitby1992). Moreover, in most recent patient reports the inflammation has been described to involve blood vessels giving rise to situations of systemic vasculitis (Oujamaa et al., Reference Oujamaa, Sibon, Vital and Menegon2003; Llanos et al., Reference Llanos, Soto, Sabugo, Gallegos, Valenzuela, Verdaguer and Cuchacovich2006; Málaga et al., Reference Málaga, Taco-Palma, Cáceres-Pizarro, Lazo, de los, Castaneda-Guarderas and Ticse2012).

Fasciola may use the bile salts (phospholipids, cholesterol and bilirubin) to encapsulate its excretome/secretome in a similar way as micelles are formed. As it is a haematophagous worm, Fasciola might then release these vesicles to the circulatory system. These bile salt micelles act as a transport medium to carry the excretome/secretome to the surface of the cells of the blood-brain barrier. Here, in combination with inflammation-activated kallikrein, bradykinin cascade effects are triggered, including capillary leakage. Bradykinin effects only last for a very short time, which suggests that F. hepatica may release several micelles containing its excretome/secretome to the circulatory system.

The plasma kallikrein-kinin system, also known as contact system (Fig. 5D), is a group of plasma proteins that responds to the presence of pathophysiological materials and invasive pathogens, by means of a plasma protease cascade initiated by factor XII (FXII) that activates the proinflammatory kallikrein-kinin system and the procoagulant intrinsic coagulation pathway. The plasma contact system drives proinflammatory and procoagulant pathways and is composed of plasma proteases, substrates and inhibitors produced and secreted by the liver. It consists of coagulation factors XII (FXII) and XI (FXI), plasma prekallikrein (PPK), the non-enzymatic cofactor high molecular weight kininogen (HMWK) and C1 esterase inhibitor (C1INH). FXII becomes activated when it comes into contact with negatively charged surfaces and undergoes a conformational change, which generates small amounts of activated FXII (FXIIa). FXIIa cleaves PPK to form plasma kallikrein (PK), which reciprocally activates FXII and generates a positive feedback loop of FXII activation. PK subsequently digests HMWK to release the vasoactive peptide bradykinin (BK), a potent proinflammatory peptide and the end product of the kallikrein-kinin pathway. BK activates signaling pathways resulting in increased vasodilation in arteries and veins and vascular permeability (Long et al., Reference Long, Kenne, Jung, Fuchs and Renne2016).

Contact system–dependent inflammation has been observed to be activated by multiple different pathogens, parasites included. Contact system activation is beneficial in clearing pathogens, but excessive BK formation may also have detrimental effects to the host (Long et al., Reference Long, Kenne, Jung, Fuchs and Renne2016). In patients, abundant production of BK may induce vascular leak, exerting disadvantage effects and contributing to multiple organ failure. In severe sepsis patients, it has been observed that the kinin system is activated as exemplified by elevated plasma bradykinin and consumption of prekallikrein and FXII (Wu, Reference Wu2015). In hantavirus infection, vascular leakage is caused by alterations of the endothelial barrier. Incubation of plasma proteins with hantavirus-infected endothelial cells results in HMWK cleavage, increase in enzymatic activities of FXIIa and kallikrein and liberation of bradykinin, leading to dramatic increases in endothelial cell permeability (Taylor et al., Reference Taylor, Wahl-Jensen, Copeland, Jahrling and Schmaljohn2013).

There is experimental and clinical evidence that the fibrinolytic system triggers the activation of the contact system. In vitro, it has been demonstrated that plasmin can induce FXII activation. The importance of plasmin as a natural FXII activator is also supported by observations in patients in whom neurological affection is presumably mediated via plasmin-dependent bradykinin generation (Hofman et al., Reference Hofman, de Maat, Hack and Maas2016).

The consideration that the contact system and fibrinolytic system are functionally linked (Fig. 5C and D) opens a field for the search of biomarkers useful to prevent neurological affection risk in fascioliasis patients. Contact system activation products in plasma may be valuable biomarkers as they may reflect recent bradykinin production, as it may be considered that bradykinin detection is complicated because it is only present in the circulation for a few seconds after it is released from HMWK, due to rapid degradation by kininases. Fibrinolytic biomarkers may also be helpful in identifying bradykinin-mediated neurological disorders (Hofman et al., Reference Hofman, de Maat, Hack and Maas2016).

Concluding remarks

Our results suggest that tPA overexpression due to the numerous plasminogen-binding proteins of the Fasciola excretome/secretome increases the permeability of the intact blood-brain barrier by a plasminogen-dependent mechanism. Blood stream passes of circulating plasmin agglomerations triggered by numerous concomitantly migrating juvenile flukes or by released inside-lesions concentrated E/S products shed by single or few adult worms may not only explain blood-brain barrier leakages and subsequent neurological manifestations in the acute and chronic phases, respectively, but also the puzzling polymorphisms and disconcerting multifocality of the neurological manifestations along the progressively changing clinical pictures in those patients (Fig. 5A–C).

This is, therefore, the first time that potential interactions between diverse mechanical parasitic situations and punctual non-imbalancing alterations of the fibrinolysis system in human infections are proposed that explain the large complexity, heterogeneity and timely variations of the neurological disorders in fascioliasis patients. Worth mentioning is that the explaining mechanisms proposed do not relate to the triggering of neurological disorders to individual subject susceptibility, neither in the acute nor in the chronic phase.

Reports of inflammation and vasculitis in fascioliasis patients presenting with neurological, meningeal and/or ophthalmic manifestations suggests that the fibrinolytic system triggers the activation of the contact system (Fig. 5A–D). The aforementioned proposal opens the door for the search of biomarkers for the distinguishing of fascioliasis patients with a risk to develop neurological affection, among both fibrinolysis system and contact system activation products in plasma. Moreover, antifibrinolytic treatments or B2 receptor antagonists should be investigated in laboratory animal models in the search of tools for preventing blood-brain barrier leakage which would potentially give rise to neurological manifestations in fascioliasis patients.

The blood-brain barrier leakage pathways linked to the fibrinolytic system and the contact system, triggered by Fasciola in neurologically affected patients, do not suggest a particular strategy by the liver fluke but merely a consequence of (i) infection by a high number of metacercariae when disorders appearing in the acute phase or by (ii) an uncommon liver parenchymal behaviour of the adult stage in the chronic phase. This appears to be different from other parasites also able to cause neurological disorders and in which matrix metalloproteinases (MMPs) have been found to be affected, modified or involved. These are species in which blood represents the final microhabitat or a crucial migration road and most of them having an evident tropism for the central nervous system. Examples are nematodiasis such as angiostrongyliasis or eosinophilic meningoencephalitis (Chiu and Lai, Reference Chiu and Lai2014) and several protozooses such as cerebral malaria (Polimeni and Prato, Reference Polimeni and Prato2014), trypanosomiasis including sleeping sickness and Chagas disease, leishmaniasis and toxoplasmic encephalitis in immunocompromised hosts (Geurts et al., Reference Geurts, Opdenakker and Van den Steen2011). A mechanism by which parasite-derived products alter host expression of MMP and endogenous MMP inhibitors has only been described for hemozoin in malaria (Prato et al., Reference Prato, D'Alessandro, Van den Steen, Opdenakker, Arese, Taramelli and Basilico2011). Although a potential role of MMPs in blood-brain barrier leakage cannot a priori be disregarded, none of the characteristics of the host-parasite inter-relationships of a biliary trematode as Fasciola suggests that way.

Financial support

Financial support for Valencia team obtained by Project No. 2017/ACDE/001583 de Innovación para el Desarrollo of the Agencia Española de Cooperación Internacional para el Desarrollo (AECID), Ministry of Foreign Affairs and Cooperation, Madrid, Spain; by Health Research Project No. PI16/00520, Subprograma Estatal de Generación de Conocimiento de la Acción Estratégica en Salud (AES), Plan Estatal de Investigación Científica y Técnica y de Innovación, ISCIII-MINECO, Madrid, Spain; by the Red de Investigación de Centros de Enfermedades Tropicales – RICET (Project No. RD16/0027/0023 of the PN de I + D + I, ISCIII-Subdirección General de Redes y Centros de Investigación Cooperativa RETICS), Ministry of Health and Consumption, Madrid; by Project No. 2016/099 of the PROMETEO Program, Programa of Ayudas para Grupos de Investigación de Excelencia, Generalitat Valenciana, Valencia, Spain; and by Project No. 2017/01 of the V Convocatoria de Proyectos de Cooperación al Desarrollo de la Universidad de Valencia de 2016, Valencia, Spain.

Financial support for Salamanca team obtained by Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad (Project AGL2015-67023-C2-2-R), Ministry of Economy, Industry and Competitiveness of the State General Administration cofinanced with FEDER Funds, Madrid and by the Red de Investigación de Centros de Enfermedades Tropicales – RICET (Project No. RD16/0027/0018 of the PN de I + D + I, ISCIII-Subdirección General de Redes y Centros de Investigación Cooperativa RETICS), Ministry of Health and Consumption, Madrid, Spain.

M. Reguera-Gomez was supported by a fellowship of the Programa de Ayudas de Formación de Profesorado Universitario 2015 (fellowship No. FPU15/03626), Ministerio de Educación, Cultura y Deporte, Madrid, Spain.

Conflict of interest

None.

Ethical standards

All animal research was performed with the approval of the Committee for the Evaluation of Projects concerning Animal Research at University of Valencia (‘Organo Habilitado para la Evaluación de Proyectos de Experimentación Animal de la Universidad de Valencia’) (2015/VSC/PEA/00001 tipo 2), strictly following the institution's guidelines based on Directive 2010/63/EU.

References

Acosta-Ferreira, W, Vercelli-Retta, J and Falconi, LM (1979) Fasciola hepatica human infection. Histopathological study of sixteen cases. Virchows Archiv, A (Pathological Anatomy and Histology) 383, 319327.Google Scholar
Acuña-Soto, R and Braun-Roth, G (1987) Bleeding ulcer in the common bile duct due to Fasciola hepatica. American Journal of Gastroenterology 82, 560562.Google Scholar
Aguirre Errasti, C, Merino Angulo, J, Flores Torres, M and De los Ríos, A (1981) Formas aberrantes de Fasciola hepatica. Estudio de dos casos. Medicina Clínica (Barcelona) 76, 125128.Google Scholar
Almendras-Jaramillo, M, Rivera-Medina, J, Seijas-Mogrovejo, J and Almendras-Jaramillo, K (1997) Fasciolasis hepática en ninos: manifestaciones clínicas poco frecuentes. Arquivos de Gastroenterología 34, 241246.Google Scholar
Arjona, R, Riancho, JA, Aguado, JM, Salesa, R and Gonzalez-Macías, J (1995) Fascioliasis in developed countries: a review of classic and aberrant forms of the disease. Medicine (Baltimore) 74, 1323.Google Scholar
Aroonroch, R, Worawichawong, S, Nitiyanant, P, Kanchanapitak, A and Bunyaratvej, S (2006) Hepatic fascioliasis due to Fasciola hepatica: a two-case report. Journal of the Medical Association of Thailand 89, 17701774.Google Scholar
Bannerman, C and Manzur, AY (1986) Fluctuating jaundice and intestinal bleeding in a 6-year-old girl with fascioliasis. Tropical and Geographical Medicine 38, 429431.Google Scholar
Berenger, F (1984) Les complications neurologiques de la distomatose à Fasciola hepatica. A propos de deux nouveaux cas (Thèse en Médecine). U.E.R. Faculté de Médeine Lyon-Nord, Université Claude Bernard – Lyon I, No. 150, 62 pp.Google Scholar
Bernal, D, de la Rubia, JE, Carrasco-Abad, AM, Toledo, R, Mas-Coma, S and Marcilla, A (2004) Identification of enolase as a plasminogen-binding protein in excretory-secretory products of Fasciola hepatica. FEBS Letters 563, 203206.Google Scholar
Bhattacharya, S, Ploplis, VA and Castellino, FJ (2012) Bacterial plasminogen receptors utilize host plasminogen system for effective invasion and dissemination. Journal of Biomedicine and Biotechnology 2012, 482096.Google Scholar
Castellino, FJ and Ploplis, VA (2005) Structure and function of the plasminogen/plasmin system. Thrombosis and Haemostasis 93, 647654.Google Scholar
Cesarman-Maus, G and Hajjar, KA (2005) Molecular mechanisms of fibrinolysis. British Journal of Haematology 129, 307321.Google Scholar
Chapin, JC and Hajjar, KA (2015) Fibrinolysis and the control of blood coagulation. Blood Reviews 29, 1724.Google Scholar
Chen, MG and Mott, KE (1990) Progress in assessment of morbidity due to Fasciola hepatica infection: a review of recent literature. Tropical Diseases Bulletion 87, R1R38.Google Scholar
Chiu, PS and Lai, SC (2014) Matrix metalloproteinase-9 leads to blood-brain barrier leakage in mice with eosinophilic meningoencephalitis caused by Angiostrongylus cantonensis. Acta Tropica 140, 141150.Google Scholar
Cwiklinski, K, Dalton, JP, Dufresne, PJ, La Course, J, Williams, DJ, Hodgkinson, J and Paterson, S (2015) The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution. Genome Biology 16, 71.Google Scholar
Dalton, JP, Robinson, MW, Mulcahy, G, O'Neill, SM and Donnelly, S (2013) Immunomodulatory molecules of Fasciola hepatica: candidates for both vaccine and immunotherapeutic development. Veterinary Parasitology 195, 272285.Google Scholar
de la Paz Santangelo, M, Gest, PM, Guerin, ME, Coinçon, M, Pham, H, Ryan, G, Puckett, SE, Spencer, JS, Gonzalez-Juarrero, M, Daher, R, Lenaerts, AJ, Schnappinger, D, Therisod, M, Ehrt, S, Sygusch, J and Jackson, M (2011) Glycolytic and non-glycolytic functions of Mycobacterium tuberculosis fructose-1,6-bisphosphate aldolase, an essential enzyme produced by replicating and nonreplicating bacilli. Journal of Biological Chemistry 286, 4021940231.Google Scholar
de la Torre-Escudero, E, Manzano-Román, R, Pérez-Sánchez, R, Siles-Lucas, M and Oleaga, A (2010) Cloning and characterization of a plasminogen-binding surface-associated enolase from Schistosoma bovis. Veterinary Parasitology 173, 7684.Google Scholar
de la Torre-Escudero, E, Manzano-Román, R, Siles-Lucas, M, Pérez-Sánchez, R, Moyano, JC, Barrera, I and Oleaga, A (2012) Molecular and functional characterization of a Schistosoma bovis annexin: fibrinolytic and anticoagulant activity. Veterinary Parasitology 184, 2536.Google Scholar
Dong, MX, Hu, QC, Shen, P, Pan, JX, Wei, YD, Liu, YY, Ren, YF, Liang, ZH, Wang, HY, Zhao, LB and Xie, P (2016) Recombinant tissue plasminogen activator induces neurological side effects independent on thrombolysis in mechanical animal models of focal cerebral infarction: a systematic review and meta-analysis. PLoS ONE 11, e0158848.Google Scholar
Fanne, RA, Nassar, T, Yarovoi, S, Rayan, A, Lamensdorf, I, Karakoveski, M, Vadim, P, Jammal, M, Cines, DB and Higazi, AAR (2010) Blood-brain barrier permeability and tPA–mediated neurotoxicity. Neuropharmacology 58, 972980.Google Scholar
Figueiredo, BC, Da'dara, AA, Oliveira, SC and Skelly, PJ (2015) Schistosomes enhance plasminogen activation: the role of tegumental enolase. PLoS Pathogens 11, e1005335.Google Scholar
Figuera, L, Gómez-Arreaza, A and Avilán, L (2013) Parasitism in optima forma: exploiting the host fibrinolytic system for invasion. Acta Tropica 128, 116123.Google Scholar
Frances, C, Piette, JC, Saada, V, Papo, T, Wechsler, B, Chosidow, O and Godeau, P (1994) Multiple subungual splinter hemorrhages in the antiphospholipid syndrome: a report of five cases and review of the literature. Lupus 3, 123128.Google Scholar
Funk, J, Schaarschmidt, B, Slesiona, S, Hallström, T, Horn, U and Brock, M (2016) The glycolytic enzyme enolase represents a plasminogen-binding protein on the surface of a wide variety of medically important fungal species. International Journal of Medical Microbiology 306, 5968.Google Scholar
Furuya, H and Ikeda, R (2011) Interaction of triosephosphate isomerase from Staphylococcus aureus with plasminogen. Microbiology and Immunology 55, 855862.Google Scholar
Gando, S (2015) Hemostasis and thrombosis in trauma patients. Seminars in Thrombosis and Hemostasis 41, 2634.Google Scholar
Geurts, N, Opdenakker, G and Van den Steen, PE (2011) Matrix metalloproteinases as therapeutic targets in protozoan parasitic infections. Pharmacology and Therapeutics 133, 257279.Google Scholar
Ghawabi, MH, Salem, SA and Azab, ME (1978) A case of ectopic fascioliasis. Journal of the Egyptian Society of Parasitology 8, 141146.Google Scholar
Girones, N, Valero, MA, Garcia-Bodelon, MA, Chico-Calero, MI, Punzon, C, Fresno, M and Mas-Coma, S (2007) Immune supression in advanced chronic fascioliasis: an experimental study in a rat model. Journal of Infectious Diseases 195, 15041512.Google Scholar
Gómez-Arreaza, A, Acosta, H, Quiñones, W, Concepción, JL, Michels, PA and Avilán, L (2014) Extracellular functions of glycolytic enzymes of parasites: unpredicted use of ancient proteins. Molecular and Biochemical Parasitology 193, 7581.Google Scholar
González-Miguel, J, Morchón, R, Mellado, I, Carretón, E, Montoya-Alonso, JA and Simón, F (2012) Excretory/secretory antigens from Dirofilaria immitis adult worms interact with the host ibrinolytic system involving the vascular endothelium. Molecular and Biochemical Parasitology 181, 134140.Google Scholar
González-Miguel, J, Morchón, R, Gussoni, S, Bossetti, E, Hormaeche, M, Kramer, LH and Simón, F (2014) Mmunoproteomic approach for identification of Ascaris suum proteins recognized by pigs with porcine ascariasis. Veterinary Parasitology 203, 343348.Google Scholar
González-Miguel, J, Morchón, R, Carretón, E, Montoya-Alonso, JA and Simón, F (2015) Can the activation of plasminogen/plasmin system of the host by metabolic products of Dirofilaria immitis participate in heartworm disease endarteritis? Parasite & Vectors 8, 194.Google Scholar
González-Miguel, J, Siles-Lucas, M, Kartashev, V, Morchón, R and Simón, F (2016) Plasmin in parasitic chronic infections: friend or foe? Trends in Parasitology 32, 325335.Google Scholar
Goodman, MA, Henderson, JI and Cullity, GJ (1973) Fascioliasis causing jaundice and intestinal bleeding. The Medical Journal of Australia 2, 547550.Google Scholar
Grange, D, Dhumeaux, D, Couzineau, P, Bismuth, H and Bader, JP (1974) Hepatic calcification due to Fasciola gigantica. Archives of Surgery 108, 113115.Google Scholar
Gur-Wahnon, D, Mizrachi, T, Maaravi-Pinto, FY, Lourbopoulos, A, Grigoriadis, N, Higazi, AAR and Brenner, T (2013) The plasminogen activator system: involvement in central nervous system inflammation and a potential site for therapeutic intervention. Journal of Neuroinflammation 10, 124.Google Scholar
Halton, DW (1997) Nutritional adaptations to parasitism within the platyhelminthes. International Journal for Parasitology 27, 693704.Google Scholar
Han, JK, Jang, HJ, Choi, BI, Kim, SH, Kim, TK, Won, HJ, Kim, YI and Cho, SY (1999) Experimental hepatobiliary fascioliasis in rabbits: a radiology-pathology correlation. Investigative Radiology 34, 99108.Google Scholar
He, L, Ren, M, Chen, X, Wang, X, Li, S, Lin, J, Liang, C, Liang, P, Hu, Y, Lei, H, Bian, M, Huang, Y, Wu, Z, Li, X and Yu, X (2014) Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products. Parasitology Research 113, 27432755.Google Scholar
Higazi, AA, El-Haj, M, Melhem, A, Horani, A, Pappo, O, Alvarez, CE, Muhanna, N, Friedman, SL and Safadi, R (2008) Immunomodulatory effects of plasminogen activators on hepatic fibrogenesis. Clinical and Experimental Immunology 152, 163173.Google Scholar
Hofman, Z, de Maat, S, Hack, CE and Maas, C (2016) Bradykinin: inflammatory product of the coagulation system. Clinical Reviews in Allergy and Immunology 51, 152161.Google Scholar
Hu, Y, Zhang, E, Huang, L, Li, W, Liang, P, Wang, X, Xu, J, Huang, Y and Yu, X (2014) Expression profiles of glyceraldehyde-3-phosphate dehydrogenase from Clonorchis sinensis: a glycolytic enzyme with plasminogen binding capacity. Parasitology Research 113, 45434553.Google Scholar
Jin, X, Liu, J and Liu, W (2014) Early ischemic blood brain barrier damage: a potential indicator for hemorrhagic transformation following tissue plasminogen activator (tPA) thrombolysis? Current Neurovascular Research 11, 254262.Google Scholar
Kim, AJ, Choi, CH, Choi, SK, Shin, YW, Park, YK, Kim, L, Choi, SJ, Han, JY, Kim, JM, Chu, YC and Park, IS (2015) Ectopic human Fasciola hepatica infection by an adult worm in the Mesocolon. Korean Journal of Parasitology 53, 725730.Google Scholar
Lesecq, R, Gnamey, D, Dubois, B, Farriaux, JP, Vernes, A, Capron, A and Fontaine, G (1972) Une forme neurologique exceptionnelle de distomatose à F. hepatica chez l'enfant. Annales de Pédiatrie, Paris 19, 885888.Google Scholar
Li, J (2005) Multifunctional Roles of Plasmin in Inflammation. Umeå, Sweden: Department of Medical Biochemistry and Biophysics, Umeå University, New Series No. 936. pp. 180.Google Scholar
Lin, L and Hu, K (2014) Tissue plasminogen activator and inflammation: from phenotype to signaling mechanisms. American Journal of Clinical and Experimental Immunology 3, 3036.Google Scholar
Llanos, C, Soto, l, Sabugo, F, Gallegos, I, Valenzuela, O, Verdaguer, J and Cuchacovich, M (2006) Systemic vasculitis associated with Fasciola hepatica infection. Scandinavian Journal of Rheumatology 35, 143146.Google Scholar
Loja, D, Alvizuri, J, Vilca, M, Aviles, R and Sanchez, M (2003) Hematoma hepático subcapsular por Fasciola. Revista de Gastoenterología del Perú 23, 142148.Google Scholar
Long, AT, Kenne, E, Jung, R, Fuchs, TA and Renne, T (2016) Contact system revisited: an interface between inflammation, coagulation, and innate immunity. Journal of Thrombosis and Haemostasis 14, 427437.Google Scholar
Lortat-Jacob, JL, Maillard, JN, Negevand, R and Camus, JP (1960) Hepactectomie droite élargie reglée d'urgence pour hemorragie souscapsulaire du foie. Un cas rare de distomatose hépatique. Mémoires de l'Académie de Chirurgie 86, 7781.Google Scholar
Málaga, G, Taco-Palma, R, Cáceres-Pizarro, J, Lazo, M de los, A, Castaneda-Guarderas, A and Ticse, R (2012) Vasculitis secundaria a infección por Fasciola hepatica. Revista Peruana de Medicina Experimental y Salud Publica 29, 386389.Google Scholar
Marcilla, A, Pérez-García, A, Espert, A, Bernal, D, Muñoz-Antolí, C, Esteban, JG and Toledo, R (2007) Echinostoma caproni: identification of enolase in excretory/secretory products, molecular cloning, and functional expression. Experimental Parasitology 117, 5764.Google Scholar
Marcos-Contreras, OA, Martinez de Lizarrondo, S, Bardou, I, Orset, C, Pruvost, M, Anfray, A, Frigout, Y, Hommet, Y, Lebouvier, L, Montaner, J, Denis Vivien, D and Gauberti, M (2016) Hyperfibrinolysis increases blood–brain barrier permeability by a plasmin- and bradykinin-dependent mechanism. Blood 128, 24232434.Google Scholar
Martinez, K, Rodriguez, R, Pila-Perez, R, Pila-Pelaez, R and Tamakloe, K (2002) Hematoma hepático por fascioliasis. Gaceta Médica de México 138, 271274.Google Scholar
Mas-Coma, S (2005) Epidemiology of fascioliasis in human endemic areas. Journal of Helminthology 79, 207216.Google Scholar
Mas-Coma, S, Funatsu, IR and Bargues, MD (2001) Fasciola hepatica and lymnaeid snails occurring at very high altitude in South America. Parasitology 123, S115S127.Google Scholar
Mas-Coma, S, Valero, MA and Bargues, MD (2009) Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Advances in Parasitology 69, 41146.Google Scholar
Mas-Coma, S, Bargues, MD and Valero, MA (2014 a) Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario. Parasitology 141(Special Issue), 19181946.Google Scholar
Mas-Coma, S, Agramunt, VH and Valero, MA (2014 b) Neurological and ocular fascioliasis in humans. Advances in Parasitology 84, 27149.Google Scholar
Mas-Coma, S, Bargues, MD and Valero, MA (2018) Human fascioliasis infection sources, their diversity, incidence factors, analytical methods and prevention measures. Parasitology (Special Issue), 1–35. https://doi.org/10.1017/S0031182018000914.Google Scholar
McVeigh, P, Maule, AG, Dalton, JP and Robinson, MW (2012) Fasciola hepatica virulence-associated cysteine peptidases: a systems biology perspective. Microbes and Infection 14, 301310.Google Scholar
Mebius, MM, van Genderen, PJ, Urbanus, RT, Tielens, AG, de Groot, PG and van Hellemond, JJ (2013) Interference with the host haemostatic system by schistosomes. PLoS Pathogens 9, e1003781.Google Scholar
Meemon, K and Sobhon, P (2015) Juvenile-specific cathepsin proteases in Fasciola spp.: their characteristics and vaccine efficacies. Parasitology Research 114, 28072813.Google Scholar
Michalski, D, Hobohm, C, Weise, C, Pelz, J, Heindl, M, Kamprad, M, Kacza, J and Härtig, W (2012) Interrelations between blood-brain barrier permeability and matrix metalloproteinases are differently affected by tissue plasminogen activator and hyperoxia in a rat model of embolic stroke. Medical Gas Research 2, 2.Google Scholar
Mohammadi Ghalehbin, B, Chinifroush Asl, MM and Ramzi, F (2010) Ectopic fascioliasis or peritoneal malignancy tumors? (Case report). Iranian Journal of Parasitology 5 (suppl. issue S1), 117.Google Scholar
Morales, JL, Arriagada, R, Salas, L, Morales, C, Fuentes, F and Santana, R (2009) Hematoma subcapsular por fasciolasis. Revista Chilena de Cirugía 61, 7377.Google Scholar
Niego, B and Medcalf, RL (2014) Plasmin-dependent modulation of the blood–brain barrier: a major consideration during tPA-induced thrombolysis? Journal of Cerebral Blood Flow & Metabolism 34, 12831296.Google Scholar
Niego, B, Freeman, R, Puschmann, TB, Turnley, AM and Medcalf, RL (2012) t-PA–specific modulation of a human blood-brain barrier model involves plasmin-mediated activation of the Rho kinase pathway in astrocytes. Blood 119, 47524761.Google Scholar
Oleaga, A, Pérez-Sánchez, R, Pagés, E, Marcos-Atxutegi, C and Simón, F (2009) Identification of mmunoreactive proteins from the dog heartworm (Dirofilaria immitis) differentially recognized by the sera from dogs with patent or occult infections. Moleculaer and Biochemical Parasitology 166, 134141.Google Scholar
Oujamaa, L, Sibon, I, Vital, A and Menegon, P (2003) Vasculite cérébrale secondaire à une co-infestation par Toxocara canis et Fasciola hepatica. Revue Neurologique (Paris) 159, 447450.Google Scholar
Paraf, A, Rousset, JJ, Trad, J and Benchettrit, (1967) Distomatose hépatique à Fasciola gigantica (douve tropicale). Importance des troubles psychiques. Bulletins et Mémoires de la Sociérté Médicale des Hôpitaux de Paris 118, 13131320.Google Scholar
Park, CI, Ro, JY, Kim, H and Gutierrez, Y (1984) Human ectopic fascioliasis in the cecum. American Journál of Surgical Pathology 8, 7377.Google Scholar
Periago, MV, Valero, MA, Panova, M and Mas-Coma, S (2006) Phenotypic comparison of allopatric populations of Fasciola hepatica and Fasciola gigantica from European and African bovines using a computer image analysis system (CIAS). Parasitology Research 99, 368378.Google Scholar
Piquet, F, Elhadad, A, Slama, JL and Brassier, D (1986) Subcapsular hematoma of the liver complicating hepatic distomatosis: a case report. Value of diagnostic imaging methods and their therapeutic implications. Annales de Chirurgie 40, 653656.Google Scholar
Polimeni, M and Prato, M (2014) Host matrix metalloproteinases in cerebral malaria: new kids on the block against blood–brain barrier integrity? Fluids and Barriers of the CNS 11, 1.Google Scholar
Prato, M, D'Alessandro, S, Van den Steen, PE, Opdenakker, G, Arese, P, Taramelli, D and Basilico, N (2011) Natural haemozoin modulates matrix metalloproteinases and induces morphological changes in human microvascular endothelium. Cellular Microbiology 13, 12751285.Google Scholar
Prociv, P, Walker, JC and Whitby, M (1992) Human ectopic fascioliasis in Australia: first case reports. Medical Journal of Australia 156, 349351.Google Scholar
Ragab, M and Farag, HF (1978) On human fascioliasis in Egypt. Journal of the Egyptian Medical Association 61, 773780.Google Scholar
Ramajo-Hernández, A, Pérez-Sánchez, R, Ramajo-Martín, V and Oleaga, A (2007) Schistosoma bovis: plasminogen binding in adults and the identification of plasminogen-binding proteins from the worm tegument. Experimental Parasitology 115, 8391.Google Scholar
Rijken, DC and Lijnen, HR (2009) New insights into the molecular mechanisms of the fibrinolytic system. Journal of Thrombosis and Haemostasis 7, 413.Google Scholar
Robinson, MW, Menon, R, Donnelly, SM, Dalton, JP and Ranganathan, S (2009) An integrated transcriptomics and proteomics analysis of the secretome of the helminth pathogen Fasciola hepatica: proteins associated with invasion and infection of the mammalian host. Molecular and Cellular Proteomics 8, 18911907.Google Scholar
Rosas, H, Fiestas, C, Torres, R, Aita, G and Lozada, D (2008) Hematoma subcapsular hepático por fascioliasis. Revista Chilena de Radiología 14, 2024.Google Scholar
Roullet, S, Freyburger, G, Labrouche, S, Norisse, E, Stecken, L, Quinart, A, Laurent, C and Sztark, F (2015) Hyperfibrinolysis during liver transplantation is associated with bleeding. Thrombosis and Haemostasis 113, 11451148.Google Scholar
Saric, J, Li, JV, Utzinger, J, Wang, Y, Keiser, J, Dirnhofer, S, Beckonert, O, Sharabiani, MTA, Fonville, JM, Nicholson, JK and Holmes, E (2010) Systems parasitology: effects of Fasciola hepatica on the neurochemical profile in the rat brain. Molecular Systems Biology 6, 396.Google Scholar
Sauvage, PJ, Dhers, A, Sauzedde, JM, Bakhti, L and Filippi, PA (1996) Subcapsular hematoma of the liver complicating hepatic fascioliasis. Journal de Radiologie 77, 583584.Google Scholar
Shakur, H, Roberts, I, Bautista, R, Caballero, J, Coats, T, Dewan, Y, El-Sayed, H, Goigichaishvili, T, Gupta, S, Herrera, J, Hunt, B, Iribhogbe, P, Izurieta, M, Khamis, H, Komolafe, E, Marrero, MA, Mejia-Mantilla, J, Mirana, J, Morales, C, Olaomi, O, Olldashi, F, Perel, P, Peto, R, Romana, PV, Ravi, RR and Yutthakasemsunt, S (2010) Effects of tranexamic acid on death, vascular occlusive events, and blood transfusion in trauma patients with significant haemorrhage (CRASH-2): a randomised, placebo-controlled trial. Lancet 376, 2332.Google Scholar
Shevchenko, A, Jensen, ON, Podtelejnikov, AV, Sagliocco, F, Wilm, M, Vorm, O, Mortensen, P, Boucherie, H and Mann, M (1996) Linking genome and proteome by mass spectrometry: large-scale dentification of yeast proteins from two dimensional gels. Proceedings of the National Academy of Sciences USA 93, 1444014445.Google Scholar
Stack, C, Dalton, JP and Robinson, MW (2011) The phylogeny, structure and function of trematode cysteine proteases, with particular emphasis on the Fasciola hepatica cathepsin L family. Advances in Experimental Medicine and Biology 712, 116135.Google Scholar
Stie, J, Bruni, G and Fox, D (2009) Surface-associated plasminogen binding of Cryptococcus neoformans promotes extracellular matrix invasion. PLoS ONE 4, e5780.Google Scholar
Sukhdeo, MVK and Sukhdeo, SC (1989) Gastrointestinal hormones: environmental cues for Fasciola hepatica. Parasitology 98, 239243.Google Scholar
Sukhdeo, MVK, Sangster, NC and Mettrick, DF (1988) Permanent feeding sites for adult Fasciola hepatica in rabbits? International Journal for Parasitology 18, 509512.Google Scholar
Suzuki, Y, Nagai, N and Umemura, K (2016) A review of the mechanisms of blood-brain barrier permeability by tissue-type plasminogen activator treatment for cerebral ischemia. Frontiers in Cellular Neuroscience 10, 2 (eCollection: doi: 10.3389/fncel.2016.00002).Google Scholar
Syrovets, T, Lunov, O and Simmet, T (2012) Plasmin as a proinflammatory cell activator. Journal of Leukocyte Biology 92, 509519.Google Scholar
Taylor, SL, Wahl-Jensen, V, Copeland, AM, Jahrling, PB and Schmaljohn, CS (2013) Endothelial cell permeability during hantavirus infection involves factor XII-dependent increased activation of the kallikrein-kinin system. PLoS Pathogens 9, e1003470.Google Scholar
Torgerson, P and Claxton, J (1999) Epidemiology and control. In Dalton, JP (ed), Fasciolosis. Wallingford, United Kingdom: CAB International Publishing, pp. 113149.Google Scholar
Valero, MA, Panova, M, Comes, AM, Fons, R and Mas-Coma, S (2002) Patterns in size and shedding of Fasciola hepatica eggs by naturally and experimentally infected murid rodents. Journal of Parasitology 88, 308313.Google Scholar
Valero, MA, Santana, M, Morales, M, Hernandez, JL and Mas-Coma, S (2003) Risk of gallstone disease in advanced chronic phase of fascioliasis: an experimental study in a rat model. Journal of Infectious Diseases 188, 787793.Google Scholar
Valero, MA, Navarro, M, Garcia-Bodelon, MA, Marcilla, A, Morales, M, Garcia, JE, Hernandez, JL and Mas-Coma, S (2006) High risk of bacterobilia in advanced experimental chronic fasciolosis. Acta Tropica 100, 1723.Google Scholar
Valero, MA, Girones, N, Garcia-Bodelon, MA, Periago, MV, Chico-Calero, I, Khoubbane, M, Fresno, M and Mas-Coma, S (2008) Anaemia in advanced chronic fasciolosis. Acta Tropica 108, 3543.Google Scholar
Valero, MA, Bargues, MD, Khoubbane, M, Artigas, P, Quesada, C, Berinde, L, Ubeira, FM, Mezo, M, Hernandez, JL, Agramunt, VH and Mas-Coma, S (2016) Higher physiopathogenicity by Fasciola gigantica than by the genetically close F. hepatica: experimental long-term follow-up of biochemical markers. Transactions of the Royal Society of Tropical Medicine and Hygiene 110(Special Issue), 5566.Google Scholar
Valero, MA, Perez-Crerspo, I, Chillon-Marinas, C, Khoubbane, M, Quesada, C, Reguera-Gomez, M, Mas.-Coma, S, Frersno, M and Girones, N (2017) Fasciola hepatica reinfection potentiates a mixed Th1/Th2/Th17/Treg response and correlates with the clinical phenotypes of anemia. PLoS ONE 12, e0173456.Google Scholar
Vassalli, JD, Sappino, AP and Belin, D (1991) The plasminogen activator/plasmin system. Journal of Clinical Investigation 88, 10671072.Google Scholar
Wang, YF, Tsirka, SE, Strickland, S, Stieg, PE, Soriano, SG and Lipton, SA (1998) Tissue plasminogen activator (tPA) increases neuronal damage after focal cerebral ischemia in wild-type and tPA-deficient mice. Nature Medicine 4, 228231.Google Scholar
Wang, X, Chen, W, Hu, F, Deng, C, Zhou, C, Lv, X, Fan, Y, Men, J, Huang, Y, Sun, J, Hu, D, Chen, J, Yang, Y, Liang, C, Zheng, H, Hu, X, Xu, J, Wu, Z and Yu, X (2011) Clonorchis sinensis enolase: identification and biochemical characterization of a glycolytic enzyme from excretory/secretory products. Molecular and Biochemical Parasitology 177, 135142.Google Scholar
Wu, Y (2015) Contact pathway of coagulation and inflammation. Thrombosis Journal 13, 17.Google Scholar
Yang, CD, Hwang, KK, Yan, W, Gallagher, K, FitzGerald, J, Grossman, JM, Hahn, BH and Chen, PP (2004) Identification of anti-plasmin antibodies in the antiphospholipid syndrome that inhibit degradation of fibrin. Journal of Immunology 172, 57655773.Google Scholar
Yang, J, Qiu, C, Xia, Y, Yao, L, Fu, Z, Yuan, C, Feng, X and Lin, J (2010) Molecular cloning and functional characterization of Schistosoma japonicum enolase which is highly expressed at the schistosomulum stage. Parasitology Research 107, 667677.Google Scholar
Yepes, M, Sandkvist, M, Moore, EG, Bugge, TH, Strickland, DK and Lawrence, DA (2003) Tissue-type plasminogen activator induces opening of the blood-brain barrier via the LDL receptor–related protein. Journal of Clinical Investigation 112, 15331540.Google Scholar
Ying, M, Xiaosu, H and Bin, W (2007) A case of ectopic parasitism: Fasciola hepatica larvae burrow through a human brain and mimic cerebral aneurysm. Transactions of the Royal Society of Tropical Medicine and Hygiene 101, 10511052.Google Scholar
Zhang, X, Polavarapu, R, She, H, Mao, Z and Yepes, M (2007) Tissue-type plasminogen activator and the low-density lipoprotein receptor-related protein mediate cerebral ischemia-induced nuclear factor-kappaB pathway activation. American Journal of Pathology 171, 12811290.Google Scholar
Zhang, C, An, J, Strickland, DK and Yepes, M (2009) The low-density lipoprotein receptor-related protein 1 mediates tissue-type plasminogen activator-induced microglial activation in the ischemic brain. American Journal of Pathology 174, 586594.Google Scholar
Zhou, L, Luo, L, You, C, Wang, B, Xu, J, Liao, L, Hui, X and Cai, B (2008) Multiple brain hemorrhages and hematomas associated with ectopic fascioliasis in brain and eye. Surgical Neurology 69, 516521.Google Scholar
Figure 0

Fig. 1. Plasminogen binding to 1 µg of FhES extract measured over a range of plasminogen amounts using a microtitre plate method: (■) Incubation with increasing amounts of plasminogen, 0–3 µg. (●) Competition assay with 50 mm εACA included during plasminogen incubation. (▲) Negative control consisted of wells coated only with BSA. Each point is the mean of three replicates ± s.d. The asterisk (*) designates significant (P < 0.05) differences.

Figure 1

Fig. 2. Plasminogen activation and plasmin generation by FhES extract of Fasciola hepatica: (□) 15 ng of t-PA was added to mixtures containing 2 µg of human plasminogen, 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride (Sigma) and 1 µg of FhES extract (or BSA as negative control) in the presence or absence of 50 mm of εACA in a test volume of 100 µL. (■) No t-PA was added to reaction mixtures. Each point is the mean of three replicates ± s.d. The asterisk (*) designates significant (P < 0.05) differences.

Figure 2

Fig. 3. Two-dimensional electrophoresis of the FhES and ligand blotting with plasminogen: (A) Representative 2-DE of 60 µg of the FhES extract from adult F. hepatica flukes. The gels were in the 3–10 pH range, 12% polyacrylamide and silver-stained. (B) Ligand blotting assay to determine which proteins of FhES extract bind plasminogen. The plasminogen-binding spots revealed are circled and numbered. Reference molecular masses are indicated on the left.

Figure 3

Table 1. Plasminogen-binding spots of the FhES extract identified by MALDI-TOF/TOF or LC–MS/MS

Figure 4

Fig. 4. Quantitative analyses of the FhES extract by Sypro Ruby florescent dye: Representative 2-DE of 60 µg of the FhES extract from adult F. hepatica flukes. The gels were in the 3–10 pH range, 12% polyacrylamide and Sypro Ruby-stained. Note density of proteins as the appearance of clear spots on a dark background, which is directly proportional to the amount of each protein into the gel. The plasminogen-binding spots revealed on the ligand blotting assay are circled and numbered.

Figure 5

Fig. 5. Schematic representation of the interaction between infecting Fasciola behaviour, fibrinolysis system alterations triggering subsequent blood-brain barrier leakages and contact system alterations inducing systemic vasculitis: (A) In the acute phase of the disease in cases of many simultaneously migrating, small-sized juvenile flukes after ingestion of numerous metacercariae. (B) In the chronic phase of the disease after the release of large amounts of accumulated excretory/secretory products following the breakage of encapsulating formations triggered by single worm tracks at different times. (C) Blood-brain barrier leakages subsequently occurring due to fibrinolytic system-dependent mechanism involving plasmin-dependent generation of bradykinin and subsequent activation of bradykinin B2 receptors, according to different plasminogen-binding protein agglomeration waves. (D) Inflammation and dilation of blood vessels due to contact system-dependent generation of the proinflammatory peptide bradykinin. Schema design and drawing by S. Mas-Coma.