Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-29T10:41:16.149Z Has data issue: false hasContentIssue false
  • English
  • Français

Individual differences in the distribution of sperm acrosome-associated 1 proteins among male patients of infertile couples; their possible impact on outcomes of conventional in vitro fertilization

Published online by Cambridge University Press:  17 May 2016

K. Kishida ,
H. Harayama ,
F. Kimura  and
T. Murakami
K. Kishida*
Affiliation:
Laboratory of Reproductive Biology, Division of Animal Science, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1 Rokko-dai, Nada, Kobe 657–8501, Japan. Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520–2192, Japan.
H. Harayama*
Affiliation:
Laboratory of Reproductive Biology, Division of Animal Science, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1 Rokko-dai, Nada, Kobe 657–8501, Japan.
F. Kimura
Affiliation:
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520–2192, Japan.
T. Murakami
Affiliation:
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520–2192, Japan.
*
All correspondence to: Kazumi Kishida or Hiroshi Harayama. Laboratory of Reproductive Biology, Division of Animal Science, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1 Rokko-dai, Nada, Kobe 657–8501, Japan. Tel: +81 78 803 6552. Fax: +81 78 803 5807. E-mail: [email protected] (KK) or [email protected] (HH)
All correspondence to: Kazumi Kishida or Hiroshi Harayama. Laboratory of Reproductive Biology, Division of Animal Science, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1 Rokko-dai, Nada, Kobe 657–8501, Japan. Tel: +81 78 803 6552. Fax: +81 78 803 5807. E-mail: [email protected] (KK) or [email protected] (HH)
Get access Rights & Permissions [Opens in a new window]

Summary

The aims of this study were to show the existence of individual differences in the distribution of sperm acrosome-associated 1 (SPACA1) among male patients of infertile couples and to examine their possible impact on the outcomes of conventional in vitro fertilization (IVF). The spermatozoa were collected from male patients of infertile couples, washed by centrifugation, collected by the swim-up method, and then used for clinical treatments of conventional IVF. The surplus sperm samples were fixed and stained with an anti-SPACA1 polyclonal antibody for the immunocytochemistry. In the clinical IVF treatments, fertilization rates and blastocyst development rates were evaluated. The immunocytochemical observations revealed that SPACA1 were localized definitely in the acrosomal equatorial segment and variedly in the acrosomal principal segment. Specifically, the detection patterns of SPACA1 in the acrosomal principal segment could be classified into three categories: (A) strong, (B) intermediate or faint, and (C) almost no immunofluorescence. The SPACA1 indexes were largely different among male patients with the wide range from 13 to 199 points. The SPACA1 indexes were significantly correlated with developmental rates of embryos to blastocysts (r = 0.829, P = 0.00162), although they were barely associated with fertilization rates at 19 h after insemination (r = 0.289, P = 0.389). These results suggest that the distribution of SPACA1 in sperm affects the outcomes of conventional IVF. In conclusion, this study provides initial data to promote large-scale clinical investigation to demonstrate that the SPACA1 indexes are valid as molecular biomarkers that can predict the effectiveness of conventional IVF of infertile couples.

Keywords

AcrosomeBiomarkerBlastocystInfertilityIVFSAMP32SPACA1
Type
Research Article
Information
Zygote , Volume 24 , Issue 5 , October 2016 , pp. 654 - 661
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aitken, R.J. & Nixon, B. (2013). Sperm capacitation: a distant landscape glimpsed but unexplored. Mol. Hum. Reprod. 19, 785–93.Google Scholar
Aitken, R.J., Bronson, R., Smith, T.B. & De Iuliis, G.N. (2013). The source and significance of DNA damage in human spermatozoa; a commentary on diagnostic strategies and straw man fallacies. Mol. Hum. Reprod. 19, 475–85.CrossRefGoogle ScholarPubMed
Eddy, E.M. (2006). The spermatozoon. In Knobil and Neill's Physiology of Reproduction, 3rd edn (ed. Neill, J.D.), pp. 3–54. St. Louis: Elsevier Academic Press.Google Scholar
Fujihara, Y., Satouh, Y., Inoue, N., Isotani, A., Ikawa, M. & Okabe, M. (2012). SPACA1-deficient male mice are infertile with abnormally shaped sperm heads reminiscent of globozoospermia. Development 139, 3583–9.Google Scholar
Gardner, D.K. & Schoolcraft, W.B. (1999). In vitro culture of human blastocysts. In Towards Reproductive Certainty: Infertility and Genetics Beyond (eds Jansen, R. & Mortimer, D.), pp. 377–388. Carnforth: Parthenon Press.Google Scholar
Hao, Z., Wolkowicz, M.J., Shetty, J., Klotz, K., Bolling, L., Sen, B., Westbrook, V.A., Coonrod, S., Flickinger, C.J. & Herr, J.C. (2002). SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein. Biol. Reprod. 66, 735–44.Google Scholar
Harayama, H. (2003). Viability and protein phosphorylation patterns of boar spermatozoa agglutinated by treatment with a cell-permeable cyclic adenosine 3′,5′-monophosphate analog. J. Androl. 24, 831–42.Google Scholar
Harayama, H., Nishijima, K., Murase, T., Sakase, M. & Fukushima, M. (2010). Relationship of protein tyrosine phosphorylation state with tolerance to frozen storage and the potential to undergo cyclic AMP-dependent hyperactivation in the spermatozoa of Japanese Black bulls. Mol. Reprod. Dev. 77, 910–21.Google Scholar
Holt, W.V. & Fazeli, A. (2010). The oviduct as a complex mediator of mammalian sperm function and selection. Mol. Reprod. Dev. 77, 934–43.Google Scholar
Holt, W.V. & Fazeli, A. (2015). Do sperm possess a molecular passport? Mechanistic insights into sperm selection in the female reproductive tract. Mol. Hum. Reprod. 21, 491–501.Google Scholar
Kawano, N., Kang, W., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado, K. & Baba, T. (2010). Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the mutant sperm are infertile in vitro . Biol. Reprod. 83, 359–69.Google Scholar
Kawano, N., Araki, N., Yoshida, K., Hibino, T., Ohnami, N., Makino, M., Kanai, S., Hasuwa, H., Yoshida, M., Miyado, K. & Umezawa, A. (2014). Seminal vesicle protein SVS2 is required for sperm survival in the uterus. Proc. Natl. Acad. Sci. USA 111, 4145–50.Google Scholar
Kishida, K., Sakase, M., Minami, K., Arai, M.M., Syoji, R., Kohama, N., Akiyama, T., Oka, A., Harayama, H. & Fukushima, M. (2015). Effects of acrosomal conditions of frozen-thawed spermatozoa on the results of artificial insemination in the Japanese Black cattle. J. Reprod. Dev. 61, 519–24.Google Scholar
McDonald, J.H. (2014). Handbook of Biological Statistics, 3rd edn. Baltimore: Sparky House Publishing.Google Scholar
Moska, N., Murray, E., Wakefield, P. & Matson, P. (2011). The staining pattern of human sperm with DiffQuik: relationship with sperm head morphology and a sperm chromatin structure assay (SCSA). Reprod. Biol. 11, 55–9.Google Scholar
Niakan, K.K., Han, J., Pedersen, R.A., Simon, C. & Pera, RA. (2012). Human pre-implantation embryo development. Development 139, 829–41.Google Scholar
Okabe, M. (2013). The cell biology of mammalian fertilization. Development 140, 4471–9.CrossRefGoogle ScholarPubMed
Simon, L., Liu, L., Murphy, K., Ge, S., Hotaling, J., Aston, K.I., Emery, B. & Carrell, DT. (2014). Comparative analysis of three sperm DNA damage assays and sperm nuclear protein content in couples undergoing assisted reproduction treatment. Hum. Reprod. 29, 904–17.Google Scholar
Steptoe, P.C. & Edwards, R.G. (1978). Birth after the reimplantation of a human embryo. Lancet 312, 366.Google Scholar
Swann, K. & Lai, F.A. (2013). PLCζ and the initiation of Ca2+ oscillations in fertilizing mammalian eggs. Cell Calcium 53, 55–62.Google Scholar
Tandara, M., Bajić, A., Tandara, L., Bilić-Zulle, L., Šunj, M., Kozina, V., Goluža, T. & Jukić, M. (2014). Sperm DNA integrity testing: big halo is a good predictor of embryo quality and pregnancy after conventional IVF. Andrology 2, 678–86.Google Scholar
Tavares, R.S., Silva, A.F., Lourenço, B., Almeida-Santos, T., Sousa, A.P. & Ramalho-Santos, J. (2013). Evaluation of human sperm chromatin status after selection using a modified Diff-Quik stain indicates embryo quality and pregnancy outcomes following in vitro fertilization. Andrology 1, 830–77.CrossRefGoogle ScholarPubMed
World Health Organization (2010). WHO Laboratory Manual for the Examination and Processing of Human Semen 5th edn. Geneva: WHO Press.Google Scholar
Yanagimachi, R. (1994). Mammalian fertilization. In The Physiology of Reproduction 2nd edn (eds Knobil, E. & Neill, J.D.), pp. 189–317. New York: Raven Press.Google Scholar
Yanagimachi, R. (2012). Fertilization studies and assisted fertilization in mammals: their development and future. J. Reprod. Dev. 58, 25–32.Google Scholar
Supplementary material: File

Kishida supplementary material

Figures S1-S3

Download Kishida supplementary material(File)
File 513.3 KB
Supplementary material: File

Kishida supplementary material

Tables S1-S4

Download Kishida supplementary material(File)
File 15.2 KB