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In vitro development of reconstructed bovine embryos and fate of donor mitochondria following nuclear injection of cumulus cells

Published online by Cambridge University Press:  06 August 2001

Jeong Tae Do ,
Kwon Ho Hong ,
Bo Yon Lee ,
Seung Bo Kim ,
Nam-Hyung Kim ,
Hoon Taek Lee  and
Kil Saeng Chung
Jeong Tae Do
Affiliation:
Animal Resources Research Center, Konkuk University, Kwangjin-gu, Seoul, Korea. Infertility Clinic, Department of Obstetrics and Gynaecology, Kyung Hee University Medical Center, Dongdaemungu, Seoul, Korea.
Kwon Ho Hong
Affiliation:
Animal Resources Research Center, Konkuk University, Kwangjin-gu, Seoul, Korea.
Bo Yon Lee
Affiliation:
Infertility Clinic, Department of Obstetrics and Gynaecology, Kyung Hee University Medical Center, Dongdaemungu, Seoul, Korea.
Seung Bo Kim
Affiliation:
Infertility Clinic, Department of Obstetrics and Gynaecology, Kyung Hee University Medical Center, Dongdaemungu, Seoul, Korea.
Nam-Hyung Kim
Affiliation:
Department of Animal Science, Chungbuk National University, Cheongju, Korea.
Hoon Taek Lee
Affiliation:
Animal Resources Research Center, Konkuk University, Kwangjin-gu, Seoul, Korea.
Kil Saeng Chung
Affiliation:
Animal Resources Research Center, Konkuk University, Kwangjin-gu, Seoul, Korea.
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Abstract

In this study we examined the developmental potential of reconstructed embryos and the fate of donor mitochondria during preimplantation development after nuclear transfer in cattle. Isolated cumulus cells were used as donor cells in nuclear transfer. Cumulus cells labelled with MitoTracker Green FM fluorochrome were injected into enucleated bovine MII oocytes and cultured in vitro. MitoTracker labelling on donor cells did not have a detrimental effect on blastocyst formation following nuclear transfer. Cleavage rate was about 69% (56/81) and blastocyst formation rate was 6.2% (5/81) at 7 days after nuclear transfer. The labelled mitochondria dispersed to the cytoplasm and became distributed between blastomeres and could be identified up to the 8- to 15-cell stage. Small patches of mitochondria were detected in some 8- to 15-cell stage embryos (5/20). However, donor mitochondria were not detected in embryos at the 16-cell stage and subsequent developmental stages. In the control group, mitochondria could be identified in arrested 1-cell embryos up to 7 days after nuclear transfer. These results suggest that disappearance of the labelled donor mitochondria in nuclear transfer bovine embryos is not due to fading of the fluorochrome marker, but is rather an as yet undefined cytoplasmic event.

Keywords

Bovine embryosCumulus cellMitochondriaNuclear transfer
Type
Research Article
Information
Zygote , Volume 9 , Issue 3 , August 2001 , pp. 211 - 218
Copyright
© 2001 Cambridge University Press

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