Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-28T11:08:14.630Z Has data issue: false hasContentIssue false
  • English
  • Français

Finding of bands of higher molecular weight than expected in three proteins in bovine preimplantation embryos

Published online by Cambridge University Press:  11 June 2019

Veronika Kinterova Open the ORCID record for Veronika Kinterova [Opens in a new window] ,
Veronika Petruskova ,
Jiri Kanka  and
Tereza Toralova
Veronika Kinterova*
Affiliation:
Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v.v.i., Libechov, Czech Republic Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic
Veronika Petruskova
Affiliation:
Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v.v.i., Libechov, Czech Republic Faculty of Science, Charles University in Prague, Prague, Czech Republic
Jiri Kanka
Affiliation:
Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v.v.i., Libechov, Czech Republic
Tereza Toralova
Affiliation:
Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v.v.i., Libechov, Czech Republic
*
*Address for correspondence: Veronika Kinterova. Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v. v. i., Rumburska 89, 277 21 Libechov, Czech Republic. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

We report here the existence of bands of higher molecular weight after western blot analysis in three proteins – Skp1, p27 and IκBα in bovine preimplantation embryos. This finding is specific to preimplantation embryos (from the 2-cell stage to the blastocyst stage) and not differentiated fibroblast cells in which these bands were of expected molecular weight. We suggest that these bands of higher molecular weight represent a complex of proteins that are characteristic of preimplantation embryos.

Keywords

IκBαMolecular weightp27Preimplantation embryoSkp1
Type
Short Communication
Information
Zygote , Volume 27 , Issue 3 , June 2019 , pp. 187 - 189
Copyright
© Cambridge University Press 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bai, C, Sen, P, Hofmann, K, Ma, L, Goebl, M, Harper, JW and Elledge, SJ (1996) SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86, 263–274.Google Scholar
Benesova, V, Kinterova, V, Kanka, J and Toralova, T (2016) Characterization of SCF complex during bovine preimplantation development. PLoS One 11, e0147096.Google Scholar
Duesbery, NS, Choi, T, Brown, KD, Wood, KW, Resau, J, Fukasawa, K, Cleveland, DW and Vande Woude, GF (1997) CENP-E is an essential kinetochore motor in maturing oocytes and is masked during Mos-dependent, cell cycle arrest at metaphase II. Proc Natl Acad Sci USA 94, 9165–9170.Google Scholar
Lei, L, Xukun, L and Jurrien, D (2013) The maternal to zygotic transition in mammals. Mol Aspects Med 34, 919–938.Google Scholar
Li, L, Baibakov, B and Dean, J (2008) A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev Cell 15, 416–425.Google Scholar
Ohsugi, M, Zheng, P, Baibakov, B, Li, L and Dean, J (2008) Maternally derived FILIA-MATER complex localizes asymmetrically in cleavage-stage mouse embryos. Development 135, 259–269.Google Scholar
Piva, R, Liu, J, Chiarle, R, Podda, A, Pagano, M and Inghirami, G (2002) In vivo interference with Skp1 function leads to genetic instability and neoplastic transformation. Mol Cell Biol 22, 8375–8387.Google Scholar
Slingerland, J and Pagano, M (2000) Regulation of the cdk inhibitor p27 and its deregulation in cancer. J Cell Physiol 183, 10–17.Google Scholar
Svarcova, O, Laurincik, J, Avery, B, Mlyncek, M, Niemann, H and Maddox-Hyttel, P (2007) Nucleolar development and allocation of key nucleolar proteins require de novo transcription in bovine embryos. Mol Reprod Dev 74, 1428–1435.Google Scholar
Tay, TL, Lin, Q, Seow, TK, Tan, KH, Hew, CL and Gong, Z (2006) Proteomic analysis of protein profiles during early development of the zebrafish, Danio rerio. Proteomics 6, 3176–3188.Google Scholar
Toralova, T, Benesova, V, Vodickova Kepkova, K, Vodicka, P, Susor, A and Kanka, J (2012) Bovine preimplantation embryos with silenced nucleophosmin mRNA are able to develop until the blastocyst stage. Reprod Camb Engl 144, 349–359.Google Scholar
Zheng, JY, Yang, XM, Harrell, JM, Ryzhikov, S, Shim, EH, Lykke-Andersen, K, Wei, N, Sun, H, Kobayashi, R and Zhang, H (2002) CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex. Mol Cell 10, 1519–1526.Google Scholar