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Expression of IGF2 and IGF receptor mRNA in bovine nuclear transferred embryos

Published online by Cambridge University Press:  27 November 2003

Dong-Wook Han ,
Sang-Jin Song ,
Sang Jun Uhum ,
Jeong-Tae Do ,
Nam-Hyung Kim ,
Kil-Saeng Chung  and
Hoon Taek Lee
Dong-Wook Han
Affiliation:
Department of Animal Science, ARRC, Bio/Molecular Informatics Center, Konkuk University, Seoul, Korea
Sang-Jin Song
Affiliation:
Department of Animal Science, ARRC, Bio/Molecular Informatics Center, Konkuk University, Seoul, Korea Samsung Cheil Hospital, Reproductive Biology and Infertility Lab, Seoul, Korea
Sang Jun Uhum
Affiliation:
Department of Animal Science, ARRC, Bio/Molecular Informatics Center, Konkuk University, Seoul, Korea
Jeong-Tae Do
Affiliation:
Department of Animal Science, ARRC, Bio/Molecular Informatics Center, Konkuk University, Seoul, Korea
Nam-Hyung Kim
Affiliation:
Department of Animal Science, Research Center for Bioresource and Health, Chungbuk National University, Chungju, Korea
Kil-Saeng Chung
Affiliation:
Department of Animal Science, ARRC, Bio/Molecular Informatics Center, Konkuk University, Seoul, Korea
Hoon Taek Lee
Affiliation:
Department of Animal Science, ARRC, Bio/Molecular Informatics Center, Konkuk University, Seoul, Korea
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Abstract

Incomplete reprogramming of the donor cell nucleus after nuclear transfer (NT) probably leads to the abnormal expression of developmentally important genes. This may be responsible for the low efficiency of cloned animal production. Insulin-like growth factor 2 (IGF2) and IGF2 receptor (IGF2R) are imprinted genes that play important roles in preimplantation development. To obtain an insight into abnormal gene expression after nuclear transfer, we assessed the transcription patterns of IGF2-IGF2R in single in vitro fertilised and cloned embryos by reverse-transcription polymerase chain reaction (RT-PCR). IGF2R expression did not differ significantly but IGF2 was more highly expressed in cloned embryos than in IVF embryos (p < 0.05). This was confirmed by a quantitative RT-PCR method. Thus, incomplete reprogramming may induce abnormal transcription of IGF2 in cloned embryos.

Keywords

Gene expressionIGF2-IGF2RImprintingNTReprogramming
Type
Research Article
Information
Zygote , Volume 11 , Issue 3 , August 2003 , pp. 245 - 252
Copyright
2003 Cambridge University Press

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