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Co-culture of buffalo (Bubalus bubalis) preantral follicles with antral follicles: a comparative study of developmental competence of oocytes derived from in vivo developed and in vitro cultured antral follicles

Published online by Cambridge University Press:  18 January 2012

G. Taru Sharma ,
Pawan K. Dubey ,
Amar Nath  and
G. Saikumar
G. Taru Sharma*
Affiliation:
Reproductive Physiology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar-243122, India.
Pawan K. Dubey
Affiliation:
Reproductive Physiology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar-243122, India.
Amar Nath
Affiliation:
Reproductive Physiology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar-243122, India.
G. Saikumar
Affiliation:
Reproductive Physiology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar-243122, India. Cytopathology Laboratory, Division of Pathology, Indian Veterinary Research Institute, Izatnagar-243122, India.
*
All correspondence to: G. Taru Sharma. Reproductive Physiology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar-243122, India Tel: +91 941 2603840 (M). Telefax: +91 581 2301327 (O). e-mail: [email protected]
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Summary

The present study was undertaken to examine whether the presence of antral follicles (AFs) affects the survival, growth and steroidogenesis of preantral follicles (PFs) and compare the maturation and developmental competence of buffalo oocytes derived from in vivo developed and in vitro cultured AFs. Two experiments were carried out. In experiment I, PFs (200–250 μm) were isolated and cultured with or without AFs (3–5 mm) in TCM-199 medium that contained 10% fetal bovine serum (FBS), 1% insulin transferin selenium (ITS), 20 ng/ml epidermal growth factor (EGF), 0.5 μg/ml follicle-stimulating hormone (FSH) and 100 ng/ml insulin-like growth factor (IGF)-I. In experiment II, in vitro developmental competence was compared for the cumulus–oocyte complexes (COCs) recovered from in vivo developed and in vitro cultured AFs. Survival, growth, development of antrum, accumulation of estradiol and progesterone was (P < 0.05) higher when PFs were co-cultured with AFs. Developmental competence of both types of follicular oocytes did not differ significantly in terms of maturation and cleavage rate, but morula and blastocyst production rate were (P < 0.05) higher with in vivo developed AFs as compared with the in vitro cultured antral follicular oocytes. In conclusion, co-culture of PFs with AFs supports long-term survival and growth of buffalo PFs and this co-culture system plays a dual role for in vitro production of embryos as well as understanding the relationship between developing PFs and AFs.

Keywords

Antral folliclesBuffaloEmbryoOocytesPreantral follicles
Type
Research Article
Information
Zygote , Volume 21 , Issue 3 , August 2013 , pp. 286 - 294
Copyright
Copyright © Cambridge University Press 2012 

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