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N-Acetyl cysteine reduces the levels of reactive oxygen species and improves in vitro maturation of oocytes from medium-sized bovine antral follicles

Published online by Cambridge University Press:  23 September 2022

Laryssa G. Barrozo ,
Bianca R. Silva ,
Laís R.F.M. Paulino ,
Efigênia C. Barbalho ,
Danisvânia R. Nascimento ,
Francisco C. Costa ,
Ana L.P.S. Batista ,
Everton P.F. Lopes ,
Ana P.R. Rodrigues  and
José R.V. Silva Open the ORCID record for José R.V. Silva [Opens in a new window]
Laryssa G. Barrozo
Affiliation:
Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Av. Maurocélio Rocha Ponte 100, 62041–040, Sobral, CE, Brazil
Bianca R. Silva
Affiliation:
Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Av. Maurocélio Rocha Ponte 100, 62041–040, Sobral, CE, Brazil
Laís R.F.M. Paulino
Affiliation:
Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Av. Maurocélio Rocha Ponte 100, 62041–040, Sobral, CE, Brazil
Efigênia C. Barbalho
Affiliation:
Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Av. Maurocélio Rocha Ponte 100, 62041–040, Sobral, CE, Brazil
Danisvânia R. Nascimento
Affiliation:
Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Av. Maurocélio Rocha Ponte 100, 62041–040, Sobral, CE, Brazil
Francisco C. Costa
Affiliation:
Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Av. Maurocélio Rocha Ponte 100, 62041–040, Sobral, CE, Brazil
Ana L.P.S. Batista
Affiliation:
Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Av. Maurocélio Rocha Ponte 100, 62041–040, Sobral, CE, Brazil
Everton P.F. Lopes
Affiliation:
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil
Ana P.R. Rodrigues
Affiliation:
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil
José R.V. Silva*
Affiliation:
Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Av. Maurocélio Rocha Ponte 100, 62041–040, Sobral, CE, Brazil
*
Author for correspondence: José R.V. Silva. Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Av. Maurocélio Rocha Ponte 100, 62041–040, Sobral, CE, Brazil. E-mail: [email protected]
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Summary

This study aims to evaluate the effects of N-acetylcysteine (NAC) on bovine oocyte maturation, mitochondrial activity and transzonal projections (TZP), as well as on the levels of reactive oxygen species (ROS) and messenger RNA (mRNA) for catalase (CAT) superoxide dismutase (SOD), periredoxin-6 (Prdx6), glutathione peroxidase (GPx), growth and differentiation factor-9 (GDF9), histone H1Foo, cyclin B1 (CCNB1) and c-Mos. Bovine cumulus–oocyte complexes (COC) of medium-sized antral follicles (3.0–6.0 mm) were prematured in TCM-199 for 8 h at 38.5°C in 5% CO2. After prematuration in the presence of forskolin and C-type natriuretic peptide, COCs were matured in TCM-199 alone or with 0.1, 0.5 or 2.5 mM NAC. Then, oocytes were classified according to the stage of chromatin. Furthermore, mitochondrial activity and intracellular levels of ROS and TZP were also evaluated. The levels of mRNAs for CAT, SOD, Prdx6, GPx, GDF9, H1Foo, CCNB1 and c-Mos were evaluated using real-time polymerase chain reaction (RT-PCR). The results showed that NAC significantly increased the percentages of oocytes with resumption of meiosis when compared with those oocytes matured in control medium. Oocytes had homogeneous mitochondrial distribution, and those cultured with 0.1 and 0.5 mM NAC had lower levels of ROS when compared with the control. In addition, 0.5 mM NAC reduced TZP and the levels of mRNA for CCNB1. In contrast, NAC did not influence the expression of CAT, GPx, Prdx6, SOD, GDF9, H1Foo, and c-Mos. In conclusion, 0.5 mM NAC reduced the levels of ROS, TZP and mRNA for CCNB1, and improved in vitro resumption of meiosis in oocytes from medium-sized bovine antral follicles.

Keywords

AntioxidantsmRNAOocyte maturationROS
Type
Research Article
Information
Zygote , Volume 30 , Issue 6 , December 2022 , pp. 882 - 890
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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