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Aldehyde dehydrogenase in fresh primordial germ cells as a marker of cell ‘stemness’

Published online by Cambridge University Press:  01 February 2019

Andrea Svoradová Open the ORCID record for Andrea Svoradová [Opens in a new window] ,
Jaromír Vašíček ,
Alexander Ostró  and
Peter Chrenek
Andrea Svoradová*
Affiliation:
Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Zoology and Anthropology, Tr. A. Hlinku 1, 949 74 Nitra, Slovak Republic
Jaromír Vašíček
Affiliation:
Research Institute for Animal Production in Nitra, NPPC, Lužianky, Slovak Republic Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Science, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic
Alexander Ostró
Affiliation:
Pavol Jozef Šafárik University in Košice, Faculty of Medicine, Trieda SNP 1, 040 11, Košice, Slovak Republic
Peter Chrenek
Affiliation:
Research Institute for Animal Production in Nitra, NPPC, Lužianky, Slovak Republic Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Science, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic University of Science and Technology, Department of Animal Biochemistry and Biotechnology, Al. prof. S. Kaliskiego 7, 85-796, Bydgoszcz, Poland
*
*Address for correspondence: A. Svoradová et al. (2018) Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Zoology and Anthropology, Tr. A. Hlinku 1, 949 74 Nitra, Slovak Republic. Tel: +421 37 640 8720. E-mail: [email protected]
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Summary

Chicken primordial germ cells (PGCs) are the primary pluripotent stem cell types that will differentiate towards germ cells. High aldehyde dehydrogenase (ALDH) activity is considered as a functional marker for the detection of cell ‘stemness’. In our study the ALDEFLUOR™ kit was used for determination of ALDH activity in PGCs. PGCs were co-stained with diethylaminobenzaldehyde (DEAB) and ALDH and analyzed by flow cytometry. Our results showed a small cell population (8.0 ± 3.3%) upon preincubation of the cells with the specific inhibitor DEAB, however cells without inhibitor staining showed a fluorescence shift as an ALDH-positive population (70.5 ± 1.6%). These findings indicate higher expression of ALDH in PGCs and ALDH activity can therefore be used as a new functional marker for the detection of cell ‘stemness’ in chicken PGCs. These results may have importance for characterization of PGCs as a potential genetic resource in poultry. Further research is necessary to elucidate the role of this functional marker in these cells.

Keywords

ALDEFLUORALDHChickenFlow cytometryPrimordial germ cells
Type
Short Communication
Information
Zygote , Volume 27 , Issue 1 , February 2019 , pp. 46 - 48
Copyright
© Cambridge University Press 2019 

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References

Armstrong, L, Stojkovic, M, Dimmick, I, Ahmad, S, Stojkovic, P, Hole, N and Lako, M (2004) Phenotypic characterization of murine primitive hematopoietic progenitor cells isolated on basis of aldehyde dehydrogenase activity. Stem Cells 22, 1142–1151.Google Scholar
Burger, PE, Gupta, R, Xiong, X, Ontiveros, CS, Salm, SN, Moscatelli, D and Wilson, EL (2009) High aldehyde dehydrogenase activity: a novel functional marker of murine prostate stem/progenitor cells. Stem Cells 27, 2220–2228.Google Scholar
Cai, J, Weiss, ML and Rao, MS (2004) In search of ‘stemness’. Expl Hematol 32, 585–598.Google Scholar
Corti, S, Locatelli, F, Papadimitriou, D, Donadoni, C, Salani, S, Del Bo, R, Strazzer, S, Bresolin, N and Comi, GP (2006) Identification of a primitive brain–derived neural stem cell population based on aldehyde dehydrogenase activity. Stem Cells 24, 975–985.Google Scholar
Douville, J, Beaulieu, R and Balicki, D (2009) ALDH1 as a functional marker of cancer stem and progenitor cells. Stem Cell Dev 18, 17–26.Google Scholar
Gentry, T, Foster, S, Winstead, L, Deibert, E, Fiordalisi, M and Balber, A (2007) Simultaneous isolation of human BM hematopoietic, endothelial and mesenchymal progenitor cells by flow sorting based on aldehyde dehydrogenase activity: implications for cell therapy. Cytotherapy 9, 259–274.Google Scholar
Ginestier, C, Hur, MH, Charafe-Jauffret, E, Monville, F, Dutcher, J, Brown, M and Schott, A (2007) ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome. Cell Stem Cell 1, 555–567.Google Scholar
Huang, EH, Hynes, MJ, Zhang, T, Ginestier, C, Dontu, G, Appelman, H, Fields, JZ Wicha, MS and Boman, BM (2009) Aldehyde dehydrogenase 1 is a marker for normal and malignant human colonic stem cells (SC) and tracks SC overpopulation during colon tumorigenesis. Cancer Res 69, 3382–3389.Google Scholar
Jean, E, Laoudj‐Chenivesse, D, Notarnicola, C, Rouger, K, Serratrice, N, Bonnieu, A, Gay, S, Bacou, F, Duret, C and Carnac, G (2011) Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells. J Cell Mol Med 15, 119–133.Google Scholar
Ji, M, Guan, WJ, Gao, YH, Li, L, Bai, CY, Ma, YH and Li, XC (2016) Cultivation and biological characterization of chicken primordial germ cells. Braz Arch Biol Techn 59, e16150374.Google Scholar
Mozdziak, PE, Angerman-Stewart, J, Rushton, B, Pardue, SL and Petitte, JN (2005) Isolation of chicken primordial germ cells using fluorescence-activated cell sorting. Poult Sci 84, 594–600.Google Scholar
Naito, M, Tajima, A, Tagami, T, Yasuda, Y and Kuwana, T (1994) Preservation of chick primordial germ cells in liquid nitrogen and subsequent production of viable offspring. J Reprod Fertil 102, 321–325.Google Scholar
Nakamura, Y, Usui, F, Ono, T, Takeda, K, Nirasawa, K, Kagami, H and Tagami, T (2010) Germline replacement by transfer of primordial germ cells into partially sterilized embryos in the chicken. Biol Reprod 83, 130–137.Google Scholar
Nakamura, Y, Usui, F, Miyahara, D, Mori, T, Ono, T, Kagami, H, Takeda, K, Nirasawa, K and Tagami, T (2012) X-irradiation removes endogenous primordial germ cells (PGCs) and increases germline transmission of donor PGCs in chimeric chickens. J Reprod Dev 58, 432–437.Google Scholar
Obermair, FJ, Fiorelli, R, Schroeter, A, Beyeler, S, Blatti, C, Zoerner, B and Thallmair, M (2010) A novel classification of quiescent and transit amplifying adult neural stem cells by surface and metabolic markers permits a defined simultaneous isolation. Stem Cell Res 5, 131–143.Google Scholar
Song, Y, D’Costa, S, Pardue, SL and Petitte, JN (2005) Production of germline chimeric chickens following the administration of a busulfan emulsion. Mol Reprod Dev 70, 438–444.Google Scholar
Starvaggi Cucuzza, L, Motta, M, Miretti, S, Macchi, E, Martignani, E, Accornero, P and Baratta, M (2010) Positive effect of silymarin on cell growth and differentiation in bovine and murine mammary cells. J Anim Physiol Anim Nutr 94, 111–117.Google Scholar
Tomita, H, Tanaka, K, Tanaka, T and Hara, A (2016) Aldehyde dehydrogenase 1A1 in stem cells and cancer. Oncotarget 7, 11018.Google Scholar
Vauchez, K, Marolleau, JP, Schmid, M, Khattar, P, Chapel, A, Catelain, C, Lecourt, S Larghero, J, Fiszman, M and Vilquin, JT (2009) Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities. Mol Ther 17, 1948–1958.Google Scholar
Wang, L, Chen, MJ, Chen, DY, Peng, SF, Zhou, XL, Liao, YY and Lu, KH (2016) Derivation and characterization of primordial germ cells from Guangxi yellow-feather chickens. Poult Sci 96, 1419–1425.Google Scholar