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Revisiting auxin transport inhibition as a mode of action for herbicides

Published online by Cambridge University Press:  12 June 2017

Mani V. Subramanian ,
Sandra A. Brunn ,
Paul Bernasconi ,
Bhavesh C. Patel  and
Jeff D. Reagan
Mani V. Subramanian*
Affiliation:
Novartis Crop Protection, Research Division, 975 California Avenue, Palo Alto, CA 94304
Sandra A. Brunn
Affiliation:
Novartis Crop Protection, Research Division, Palo Alto, CA 94304
Paul Bernasconi
Affiliation:
Novartis Crop Protection, Research Division, Palo Alto, CA 94304
Bhavesh C. Patel
Affiliation:
Novartis Crop Protection, Research Division, Palo Alto, CA 94304
Jeff D. Reagan
Affiliation:
Novartis Crop Protection, Research Division, Palo Alto, CA 94304
*
[email protected].
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Abstract

SCB-1, a semicarbazone, is a phytotoxin with both preemergence and postemergence activity against broadleaves and grasses. It effectively displaced [3H]naptalam bound to a solubilized plasma membrane fraction, with the concentration required for 50% dissociation being 3.5 nM. The intrinsic dissociation constant (Kd) for binding of [3H]naptalam to zucchini plasma membranes was estimated to be 9.7 nM. Binding of [3H]naptalam to the solubilized protein fraction from the plasma membrane was rapid, time dependent, saturable, and reversible. The Kd for binding was estimated at 4 nM. Based on the maximum binding of [3H]naptalam, concentration of the binding protein was calculated to be 23.6 and 10.8 pmol mg−1 protein in the plasma membrane and solubilized fraction, respectively. SCB-1 also blocked the efflux of [14C]IAA, resulting in more than 800% accumulation of radiolabel in zucchini squash hypocotyl segments at 5 μM. This accumulation was quenched by excess cold IAA and 2,4-D. Collectively, these results suggest that SCB-1 is a potent inhibitor of auxin efflux and that it competes with naptalam for binding to the plasma membrane protein.

Keywords

Naptalam, 2-[(1-naphthalenylamino)carbonyl]benzoic acidSCB-1IAA2,4-DMode of actionauxin transportauxin transport proteinefflux carrier
Type
Symposium
Information
Weed Science , Volume 45 , Issue 5 , October 1997 , pp. 621 - 627
Copyright
Copyright © 1997 by the Weed Science Society of America 

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