Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-15T13:25:15.976Z Has data issue: false hasContentIssue false
  • English
  • Français

Mechanisms of Resistance to Pyroxsulam and ACCase Inhibitors in Japanese Foxtail (Alopecurus japonicus)

Published online by Cambridge University Press:  20 January 2017

Yujuan Feng ,
Yuan Gao ,
Yong Zhang ,
Liyao Dong  and
Jun Li
Yujuan Feng
Affiliation:
College of Plant Protection, Key Laboratory of Integrated Pest Management on Crops in East China, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China
Yuan Gao
Affiliation:
College of Plant Protection, Key Laboratory of Integrated Pest Management on Crops in East China, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China
Yong Zhang
Affiliation:
Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Liyao Dong
Affiliation:
College of Plant Protection, Key Laboratory of Integrated Pest Management on Crops in East China, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China
Jun Li*
Affiliation:
College of Plant Protection, Key Laboratory of Integrated Pest Management on Crops in East China, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China
*
Corresponding author's E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Japanese foxtail is a predominant tetraploid grass weed in wheat and oilseed rape fields in eastern China. In China, pyroxsulam is mainly used to manage annual grass weeds, especially those resistant to acetyl coenzyme A carboxylase (ACCase)-inhibiting herbicides. Using dose–response studies, a pyroxsulam-resistant population, ACTC-1, was identified with a resistance index value of 58. Additionally, ACTC-1 was cross-resistant to sulfonylureas, imidazolinones, triazolopyrimidines, pyrimidinyl-benzoates, and sulfonylaminocarbonyl-triazolinones and multiresistant to ACCase and photosystem II inhibitors. Sequence analysis revealed four gene fragments encoding acetolactate synthase (ALS) from ACTC-1, and three from JNXW-1, a pyroxsulam-sensitive population. An Asp-376-Glu substitution was found in ALS1;2 and an Ile-2041-Asn in Acc1;1, which may be responsible for its resistance to pyroxsulam and ACCase inhibitors, respectively. In vitro assays of ALS activity revealed that in ACTC-1, the sensitivity of ALS to pyroxsulam was lower, and the basal ALS activity was twofold higher than that of sensitive population JNXW-1. Additionally, the combined application of pyroxsulam with malathion or piperonyl butoxide increased the sensitivity of ACTC-1 to pyroxsulam, although it could not completely overcome the resistance. It was inferred that both target-site-based resistance and nontarget-site-based resistance may be involved in the resistance to pyroxsulam.

Keywords

Malathionpiperonyl butoxide (PBO)pyroxsulamJapanese foxtail, Alopecurus japonicas Steudbirdsrape mustard, Brassica campestris L.wheat, Triticum aestivum L.ACCaseALSnontarget-site-based resistancetarget-site-based resistance
Type
Weed Management
Information
Weed Science , Volume 64 , Issue 4 , December 2016 , pp. 695 - 704
Copyright
Copyright © 2016 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Associate Editor for this paper: Patrick J. Tranel, University of Illinois.

References

Literature Cited

Alarcon-Reverte, R, Garcia, A, Watson, SB, Abdallah, I, Sabate, S, Hernandez, MJ, Dayan, FE, Fischer, AJ (2015) Concerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California Pest Manag Sci 71:996–1007 Google Scholar
Ashigh, J, Corbett, C-AL, Smith, PJ, Laplante, J, Tardif, FJ (2009) Characterization and diagnostic tests of resistance to acetohydroxyacid synthase inhibitors due to an Asp(376)Glu substitution in Amaranthus powellii Pestic Biochem Physiol 95:38–46 Google Scholar
Beckie, HJ, Tardif, FJ (2012) Herbicide cross resistance in weeds Crop Prot 35:15–28 Google Scholar
Bi, Y, Liu, W, Guo, W, Li, L, Yuan, G, Du, L, Wang, J (2015) Molecular basis of multiple resistance to ACCase- and ALS-inhibiting herbicides in Alopecurus japonicus from China Pestic Biochem Physiol 126:22–27 Google Scholar
Bi, Y-L, Liu, W-T, Li, L-X, Yuan, G-H, Jin, T, Wang, J-X (2013) Molecular basis of resistance to mesosulfuron-methyl in Japanese foxtail, Alopecurus japonicus J Pestic Sci 38:74–77 Google Scholar
Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72:248–54Google Scholar
Chen, J, Yuan, J, Liu, J, Fu, Q, Wu, J (2005) Mechanism of action of the novel herbicide ZJ0273 Acta Phytophy Sin 32:48–52 Google Scholar
Christopher, JT, Preston, C, Powles, SB (1994) Malathion antagonizes metabolism-based chlorsulfuron resistance in Lolium rigidum Pestic Biochem Physiol 49:172–182 Google Scholar
Cocker, KM, Northcroft, DS, Coleman, JOD, Moss, SR (2001) Resistance to ACCase-inhibiting herbicides and isoproturon in UK populations of Lolium multiflorum: mechanisms of resistance and implications for control Pest Manag Sci 57:587–597 Google Scholar
Delye, C (2013) Unravelling the genetic bases of non-target-site-based resistance (NTSR) to herbicides: a major challenge for weed science in the forthcoming decade Pest Manag Sci 69:176–187 Google Scholar
Delye, C, Gardin, JAC, Boucansaud, K, Chauvel, B, Petit, C (2011) Non-target-site-based resistance should be the centre of attention for herbicide resistance research: Alopecurus myosuroides as an illustration Weed Res 51:433–437 Google Scholar
Delye, C, Michel, S, Berard, A, Chauvel, B, Brunel, D, Guillemin, JP, Dessaint, F, Le Corre, V (2010) Geographical variation in resistance to acetyl-coenzyme A carboxylase-inhibiting herbicides across the range of the arable weed Alopecurus myosuroides (black-grass) New Phytol 186:1005–1017 Google Scholar
Gressel, J (1990) Synergizing herbicides Rev Weed Sci 5:49–82 Google Scholar
Han, H, Yu, Q, Purba, E, Li, M, Walsh, M, Friesen, S, Powles, SB (2012) A novel amino acid substitution Ala-122-Tyr in ALS confers high-level and broad resistance across ALS-inhibiting herbicides Pest Manag Sci 68:1164–1170 Google Scholar
Imaizumi, T, Wang, GX, Ohsako, T, Tominaga, T (2008) Genetic diversity of sulfonylurea-resistant and -susceptible Monochoria vaginalis populations in Japan Weed Res 48:187–196 Google Scholar
Iwakami, S, Uchino, A, Watanabe, H, Yamasue, Y, Inamura, T (2012) Isolation and expression of genes for acetolactate synthase and acetyl-CoA carboxylase in Echinochloa phyllopogon, a polyploid weed species Pest Manag Sci 68:1098–1106 Google Scholar
Kwon, CS, Penner, D (1995) Response of a chlorsulfuron-resistant biotype of Kochia scoparia to ALS inhibiting herbicides and piperonyl butoxide Weed Sci 43:561–565 Google Scholar
Li, M, Yu, Q, Han, H, Vila-Aiub, M, Powles, SB (2013) ALS herbicide resistance mutations in Raphanus raphanistrum: evaluation of pleiotropic effects on vegetative growth and ALS activity Pest Manag Sci 69:689–695 Google Scholar
Massa, D, Krenz, B, Gerhards, R (2011) Target-site resistance to ALS-inhibiting herbicides in Apera spica-venti populations is conferred by documented and previously unknown mutations Weed Res 51:294–303 Google Scholar
Panozzo, S, Scarabel, L, Tranel, PJ, Sattin, M (2013) Target-site resistance to ALS inhibitors in the polyploid species Echinochloa crus-galli Pestic Biochem Physiol 105:93–101 Google Scholar
Wang, H, Li, J, Lv, B, Lou, Y, Dong, L (2013) The role of cytochrome P450 monooxygenase in the different responses to fenoxaprop-P-ethyl in annual bluegrass (Poa annua L.) and short awned foxtail (Alopecurus aequalis Sobol.) Pestic Biochem Physiol 107:334–342 Google Scholar
Warwick, SI, Xu, R, Sauder, C, Beckie, HJ (2008) Acetolactate synthase target-site mutations and single nucleotide polymorphism genotyping in ALS-resistant kochia (Kochia scoparia). Weed Sci 56:797–806 Google Scholar
Wells, GS (2008) Pyroxsulam for broad-spectrum weed control in wheat. Pages 297–299 in Proceedings of the 16th Australian Weeds Conference. Queensland: Australian Weeds Conference Google Scholar
Whaley, CM, Wilson, HP, Westwood, JH (2007) A new mutation in plant ALS confers resistance to five classes of ALS-inhibiting herbicides Weed Sci 55:83–90 Google Scholar
Xu, H, Li, J, Zhang, D, Cheng, Y, Jiang, Y, Dong, L (2014a) Mutations at codon position 1999 of acetyl-CoA carboxylase confer resistance to ACCase-inhibiting herbicides in Japanese foxtail (Alopecurus japonicus). Pest Manag Sci 70:1894–1901 Google Scholar
Xu, H, Zhang, W, Zhang, T, Li, J, Wu, X, Dong, L (2014b) Determination of ploidy level and isolation of genes encoding acetyl-CoA carboxylase in Japanese foxtail (Alopecurus japonicus). Plos one 9:e114712 Google Scholar
Xu, H, Zhu, X, Wang, H, Li, J, Dong, L (2013) Mechanism of resistance to fenoxaprop in Japanese foxtail (Alopecurus japonicus) from China Pestic Biochem Physiol 107:25–31 Google Scholar
Yu, Q, Abdallah, I, Han, H, Owen, M, Powles, S (2009) Distinct non-target site mechanisms endow resistance to glyphosate, ACCase and ALS-inhibiting herbicides in multiple herbicide-resistant Lolium rigidum Planta 230:713–723 Google Scholar
Yu, Q, Ahmad-Hamdani, MS, Han, H, Christoffers, MJ, Powles, SB (2013) Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): insights into resistance evolution in a hexaploid species Heredity 110:220–231 Google Scholar
Yu, Q, Han, H, Vila-Aiub, MM, Powles, SB (2010) AHAS herbicide resistance endowing mutations: effect on AHAS functionality and plant growth J Exp Bot 61:3925–3934 Google Scholar
Yu, Q, Powles, SB (2014) Resistance to AHAS inhibitor herbicides: current understanding Pest Manag Sci 70:1340–1350 Google Scholar
Yu, Q, Shane Friesen, LJ, Zhang, XQ, Powles, SB (2004) Tolerance to acetolactate synthase and acetyl-coenzyme A carboxylase inhibiting herbicides in Vulpia bromoides is conferred by two co-existing resistance mechanisms Pestic Biochem Physiol 78:21–30 Google Scholar
Yuan, JS, Tranel, PJ, Stewart, CN Jr (2007) Non-target-site herbicide resistance: a family business Trends Plant Sci 12:6–13 Google Scholar
Zheng, D, Kruger, GR, Singh, S, Davis, VM, Tranel, PJ, Weller, SC, Johnson, WG (2011) Cross-resistance of horseweed (Conyza canadensis) populations with three different ALS mutations Pest Manag Sci 67:1486–14 92Google Scholar