Hostname: page-component-77c89778f8-rkxrd Total loading time: 0 Render date: 2024-07-21T20:28:47.932Z Has data issue: false hasContentIssue false
  • English
  • Français

Light and Prometryne Effects on Leucine Uptake and Incorporation

Published online by Cambridge University Press:  12 June 2017

Bryan Truelove ,
Larry R. Jones  and
Donald E. Davis
Bryan Truelove
Affiliation:
Auburn Univ. Agr. Exp. Sta., Dep. of Bot. and Microbiol., Auburn, Alabama 36830
Larry R. Jones
Affiliation:
Auburn Univ. Agr. Exp. Sta., Dep. of Bot. and Microbiol., Auburn, Alabama 36830
Donald E. Davis
Affiliation:
Auburn Univ. Agr. Exp. Sta., Dep. of Bot. and Microbiol., Auburn, Alabama 36830
Get access Rights & Permissions [Opens in a new window]

Abstract

The effects on leucine uptake and its incorporation into protein by cucumber (Cucumis sativus L. ‘Ashley’) cotyledon tissue following treatments with 2,4-bis(isopropylamino)-6-(methylthio)-s-triazine (prometryne) were determined. Prometryne decreased 14C-leucine uptake both in the light and in the dark. Prometryne decreased leucine incorporation into protein only when the discs were also exposed to over 4 hr of light. Percent of absorbed leucine incorporated into protein was increased by a 4 hr exposure in the light to 10−4M prometryne following a 24-hr dark exposure to this same concentration. Similar exposure to 10~5M prometryne had no effect. Percent of leucine incorporated into protein was decreased by 10-5M prometryne following a 24 hr exposure to this same concentration in the light. However, under these conditions 10−4M prometryne had no effect. It is speculated that the increased percentage of leucine incorporated into protein may be related to increased availability of the absorbed leucine for use in protein synthesis rather than an effect of the herbicide on the rate of protein synthesis

Type
Research Article
Information
Weed Science , Volume 21 , Issue 1 , January 1973 , pp. 24 - 27
Copyright
Copyright © 1973 Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Ashton, F. M., Gifford, E. M. Jr., and Bisalputra, T. 1963. Structural changes in Phaseolus vulgaris induced by atrazine I. Histological changes. Bot. Gaz. 124:329–335.CrossRefGoogle Scholar
2. Ashton, F. M., Gifford, E. M. Jr., and Bisalputra, T. 1963. Structural changes in Phaseolus vulgaris induced by atrazine II. Effects on fine structure of chloroplasts. Bot. Gaz. 124:336–343.CrossRefGoogle Scholar
3. Copping, L. G. and Davis, D. E. 1972. Effects of atrazine on chlorophyll retention in corn leaf discs. Weed Sci. 20:86–89.Google Scholar
4. Copping, L. G., Davis, D. E., and Pillai, C. G. P. 1972. Growth regulator-like activity of atrazine and ametryne. Weed Sci. 20:274–277.Google Scholar
5. Gysin, H. and Knusli, E. 1960. Chemistry and herbicidal properties of triazine derivatives. Advan. Pest Contr. Res. 3:289–358.Google Scholar
6. Key, J. L. 1964. Ribonucleic acid and protein synthesis as essential processes for cell elongation. Plant Physiol. 39:365–370.Google Scholar
7. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.Google Scholar
8. Mann, J. D., Jordan, L. S., and Day, B. E. 1965. A survey of herbicides for their effect upon protein synthesis. Plant Physiol. 40:840–843.Google Scholar
9. McDaniel, L. and Frans, R. E. 1969. Soybean mitochondrial response to prometryne and fluometuron. Weed Sci. 17:192–196.Google Scholar
10. Moreland, D. E. and Hill, K. L. 1962. Interference of herbicides with the Hill reaction of isolated chloroplasts. Weeds 10:229–236.CrossRefGoogle Scholar
11. Moreland, D. E., Malhotra, S. S., Gruenhagen, R. D., and Shokraii, E. H. 1969. Effects of herbicides on RNA and protein synthesis. Weed Sci. 17:556–563.Google Scholar
12. Oaks, Ann, and Bidwell, R. G. S. 1970. Compartmentation of intermediary metabolites. Ann. Rev. Plant Physiol. 21:43–66.CrossRefGoogle Scholar
13. Ries, S. K., Chmiel, H., Dilley, D. R., and Filner, P. 1967. The increase in nitrate reductase activity and protein content of plants treated with simazine. Proc. Nat. Acad. Sci. 58: 526–532.Google Scholar
14. Ries, S. K. and Gast, A. 1965. The effect of simazine on nitrogenous components of corn. Weeds 13:272–274.Google Scholar
15. Shimabukuro, R. H. and Swanson, H. R. 1968. Atrazine metabolism, selectivity, and mode of action. Abstr. Pap. 155th Natn. Meet. Amer. Chem. Soc. A 24.Google Scholar
16. Shimabukuro, R. H. and Swanson, H. R. 1969. Atrazine: metabolism, selectivity and mode of action. J. Agr. Food Chem. 17:199–205.Google Scholar
17. Thompson, Olivia C., Truelove, B., and Davis, D. E. 1969. Effect of the herbicide prometryne 2,4-bis(isopropylamino)-6-(methylthio)-s-triazine on mitochondria. J. Agr. Food Chem. 17:997–999.Google Scholar
18. Venis, M. A. 1967. Effects of antibiotics on protein and ribonucleic acid synthesis in relation to the control of bacterial growth in excised tissues. Phytochemistry 6:799–806.Google Scholar